scholarly journals Expansion and Functional Diversification of SKP1-Like Genes in Wheat (Triticum aestivum L.)

2019 ◽  
Vol 20 (13) ◽  
pp. 3295
Author(s):  
Imen HajSalah El Beji ◽  
Said Mouzeyar ◽  
Mohammed-Fouad Bouzidi ◽  
Jane Roche
Keyword(s):  
Triticum Aestivum ◽  
Physcomitrella Patens ◽  
Expression Patterns ◽  
Protein S ◽  
Triticum Aestivum L ◽  
Wheat Genome ◽  
Genome Database ◽  
E3 Ligases ◽  
Expression Platform ◽  
Public Data

The ubiquitin proteasome 26S system (UPS), involving monomeric and multimeric E3 ligases is one of the most important signaling pathways in many organisms, including plants. The SCF (SKP1/Cullin/F-box) multimeric complex is particularly involved in response to development and stress signaling. The SKP1 protein (S-phase kinase-associated protein 1) is the core subunit of this complex. In this work, we firstly identified 92 and 87 non-redundant Triticum aestivum SKP1-like (TaSKP) genes that were retrieved from the latest release of the wheat genome database (International Wheat Genome Sequencing Consortium (IWGSC) RefSeq v1.0) and the genome annotation of the TGAC v1 respectively. We then investigated the structure, phylogeny, duplication events and expression patterns of the SKP1-like gene family in various tissues and environmental conditions using a wheat expression platform containing public data. TaSKP1-like genes were expressed differentially in response to stress conditions, displaying large genomic variations or short insertions/deletions which suggests functional specialization within TaSKP1-like genes. Finally, interactions between selected wheat FBX (F-box) proteins and putative ancestral TaSKP1-like proteins were tested using the yeast two-hybrid (Y2H) system to examine the molecular interactions. These observations suggested that six Ta-SKP1 genes are likely to be ancestral genes, having similar functions as ASK1 and ASK2 in Arabidopsis, OSK1 and OSK20 in rice and PpSKP1 and PpSKP2 in Physcomitrella patens.

scholarly journals Genome Wide Identification, Characterization, and Expression Analysis of YABBY-Gene Family in WHEAT (Triticum aestivum L.)

Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1189
Author(s):  
Zeeshan Ali Buttar ◽  
Yuan Yang ◽  
Rahat Sharif ◽  
Sheng Nan Wu ◽  
Yanzhou Xie ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Gene Family ◽  
Phosphorus Deficiency ◽  
Expression Patterns ◽  
Resistance Mechanism ◽  
Triticum Aestivum L ◽  
Wheat Genome ◽  
Septoria Tritici ◽  
Genome Database ◽  
Yabby Gene

The small YABBY plant-specific transcription factor has a prominent role in regulating plant growth and developmental activities. However, little information is available about YABBY gene family in Triticum aestivum L. Herein, we identified 21 TaYABBY genes in the Wheat genome database. Then, we performed the conserved motif and domain analysis of TaYABBY proteins. The phylogeny of the TaYABBY was further sub-divided into 6 subfamilies (YABBY1/YABBY3, YABB2, YABBY5, CRC and INO) based on the structural similarities and functional diversities. The GO (Gene ontology) analysis of TaYABBY proteins showed that they are involved in numerous developmental processes and showed response against environmental stresses. The analysis of all identified genes in RNA-seq data showed that they are expressed in different tissues of wheat. Differential expression patterns were observed in not only control samples but also in stressed samples such as biotic stress (i.e., Fusarium graminearum (F.g), septoria tritici (STB), Stripe rust (Sr) and Powdery mildew (Pm), and abiotic stress (i.e., drought, heat, combined drought and heat and phosphorus deficiency), especially at different grain development stages. All identified TaYABBY-genes were localized in the nucleus which implies their participation in the regulatory mechanisms of various biological and cellular processes. In light of the above-mentioned outcomes, it has been deduced that TaYABBY-genes in the wheat genome play an important role in mediating various development, growth, and resistance mechanism, which could provide significant clues for future functional studies.

Phenotype characterisation and analysis of expression patterns of genes related mainly to carbohydrate metabolism and sporopollenin in male-sterile anthers induced by high temperature in wheat (Triticum aestivum)

2018 ◽  
Vol 69 (5) ◽  
pp. 469 ◽  
Author(s):  
Hongzhan Liu ◽  
Junsheng Wang ◽  
Chaoqiong Li ◽  
Lin Qiao ◽  
Xueqin Wang ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
High Temperature ◽  
Male Sterility ◽  
Carbohydrate Metabolism ◽  
Higher Plants ◽  
Expression Patterns ◽  
Triticum Aestivum L ◽  
Male Sterile ◽  
Tetrad Stage ◽  
Expression Levels

Male reproductive development in higher plants is highly sensitive to various stressors, including high temperature (HT). In this study, physiological male-sterile plants of wheat (Triticum aestivum L.) were established using HT induction. The physiological changes and expression levels of genes mainly related to carbohydrate metabolism and sporopollenin in male-sterile processes were studied by using biological techniques, including iodine–potassium iodide staining, paraffin sectioning, scanning electron microscopy (SEM) and fluorescent quantitative analysis. Results of paraffin sectioning and SEM revealed that parts of HT male-sterile anthers, including the epidermis and tapetum, were remarkably different from those of normal anthers. The expression levels of TaSUT1, TaSUT2, IVR1 and IVR5 were significantly lower than of normal anthers at the early microspore and trinucleate stages. The RAFTIN1 and TaMS26 genes may contribute to biosynthesis and proper ‘fixation’ of sporopollenin in the development of pollen wall; however, their expression levels were significantly higher at the early tetrad stage and early microspore stage in HT sterile anthers. The recently cloned MS1 gene was expressed at the early tetrad and early microspore stages but not at the trinucleate stage. Moreover, this gene showed extremely significant, high expression in HT sterile anthers compared with normal anthers. These results demonstrate that HT induction of wheat male sterility is probably related to the expression of genes related to carbohydrate metabolism and sporopollenin metabolism. This provides a theoretical basis and technological approach for further studies on the mechanisms of HT induction of male sterility.

scholarly journals Genome-Wide Identification and Expression Profile of OSCA Gene Family Members in Triticum aestivum L.

2021 ◽  
Vol 23 (1) ◽  
pp. 469
Author(s):  
Kai Tong ◽  
Xinyang Wu ◽  
Long He ◽  
Shiyou Qiu ◽  
Shuang Liu ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Gene Family ◽  
Expression Profiles ◽  
Stomatal Closure ◽  
Expression Patterns ◽  
Family Members ◽  
Triticum Aestivum L ◽  
Free Calcium ◽  
Cis Acting ◽  
Gene Structures

Hyperosmolality and various other stimuli can trigger an increase in cytoplasmic-free calcium concentration ([Ca2+]cyt). Members of the Arabidopsis thaliana (L.) reduced hyperosmolality-gated calcium-permeable channels (OSCA) gene family are reported to be involved in sensing extracellular changes to trigger hyperosmolality-induced [Ca2+]cyt increases and controlling stomatal closure during immune signaling. Wheat (Triticum aestivum L.) is a very important food crop, but there are few studies of its OSCA gene family members. In this study, 42 OSCA members were identified in the wheat genome, and phylogenetic analysis can divide them into four clades. The members of each clade have similar gene structures, conserved motifs, and domains. TaOSCA genes were predicted to be regulated by cis-acting elements such as STRE, MBS, DRE1, ABRE, etc. Quantitative PCR results showed that they have different expression patterns in different tissues. The expression profiles of 15 selected TaOSCAs were examined after PEG (polyethylene glycol), NaCl, and ABA (abscisic acid) treatment. All 15 TaOSCA members responded to PEG treatment, while TaOSCA12/-39 responded simultaneously to PEG and ABA. This study informs research into the biological function and evolution of TaOSCA and lays the foundation for the breeding and genetic improvement of wheat.

scholarly journals Genome-wide identification and expression analysis of ADP-ribosylation factors associated with biotic and abiotic stress in wheat (Triticum aestivum L.)

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10963 ◽  
Author(s):  
Yaqian Li ◽  
Jinghan Song ◽  
Guang Zhu ◽  
Zehao Hou ◽  
Lin Wang ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Abiotic Stress ◽  
Intracellular Transport ◽  
Expression Patterns ◽  
Functional Characterization ◽  
Triticum Aestivum L ◽  
Pcr Analysis ◽  
Biotic And Abiotic Stress ◽  
Specific Expression ◽  
Wheat Varieties

The ARF gene family plays important roles in intracellular transport in eukaryotes and is involved in conferring tolerance to biotic and abiotic stresses in plants. To explore the role of these genes in the development of wheat (Triticum aestivum L.), 74 wheat ARF genes (TaARFs; including 18 alternate transcripts) were identified and clustered into seven sub-groups. Phylogenetic analysis revealed that TaARFA1 sub-group genes were strongly conserved. Numerous cis-elements functionally associated with the stress response and hormones were identified in the TaARFA1 sub-group, implying that these TaARFs are induced in response to abiotic and biotic stresses in wheat. According to available transcriptome data and qRT-PCR analysis, the TaARFA1 genes displayed tissue-specific expression patterns and were regulated by biotic stress (powdery mildew and stripe rust) and abiotic stress (cold, heat, ABA, drought and NaCl). Protein interaction network analysis further indicated that TaARFA1 proteins may interact with protein phosphatase 2C (PP2C), which is a key protein in the ABA signaling pathway. This comprehensive analysis will be useful for further functional characterization of TaARF genes and the development of high-quality wheat varieties.

scholarly journals Regulation of Glycosylphosphatidylinositol-Anchored Protein (GPI-AP) Expression by F-Box/LRR-Repeat (FBXL) Protein in Wheat (Triticum aestivum L.)

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1606
Author(s):  
Min Jeong Hong ◽  
Jin-Baek Kim ◽  
Yong Weon Seo ◽  
Dae Yeon Kim
Keyword(s):  
Triticum Aestivum ◽  
Plasma Membrane ◽  
Fluorescent Protein ◽  
Expression Patterns ◽  
Triticum Aestivum L ◽  
26S Proteasome ◽  
Bimolecular Fluorescence Complementation ◽  
Yeast Two Hybrid ◽  
Scf Complex ◽  
Two Hybrid

F-box proteins are substrate recognition components of the Skp1-Cullin-F-box (SCF) complex, which performs many important biological functions including the degradation of numerous proteins via the ubiquitin–26S proteasome system. In this study, we isolated the gene encoding the F-box/LRR-repeat (FBXL) protein from wheat (Triticum aestivum L.) seedlings and validated that the TaFBXL protein is a component of the SCF complex. Yeast two-hybrid assays revealed that TaFBXL interacts with the wheat glycosylphosphatidylinositol-anchored protein (TaGPI-AP). The green fluorescent protein (GFP) fusion protein of TaFBXL was detected in the nucleus and plasma membrane, whereas that of TaGPI-AP was observed in the cytosol and probably also plasma membrane. yeast two-hybrid and bimolecular fluorescence complementation (BiFC) assays revealed that TaFBXL specifically interacts with TaGPI-AP in the nucleus and plasma membrane, and TaGPI-AP is targeted by TaFBXL for degradation via the 26S proteasome system. In addition, TaFBXL and TaGPI-AP showed antagonistic expression patterns upon treatment with indole-3-acetic acid (IAA), and the level of TaGPI-AP was higher in tobacco leaves treated with both MG132 (proteasome inhibitor) and IAA than in leaves treated with either MG132 or IAA. Taken together, our data suggest that TaFBXL regulates the TaGPI-AP protein level in response to exogenous auxin application.

Structure of Aegilops ventricosa chromosome 6Nv, the donor of wheat genes Yr17, Lr37, Sr38, and Cre5

Genome ◽  
2005 ◽  
Vol 48 (3) ◽  
pp. 541-546 ◽  
Author(s):  
Anne-Marie Tanguy ◽  
Olivier Coriton ◽  
Paulette Abélard ◽  
Françoise Dedryver ◽  
Joseph Jahier
Keyword(s):  
Triticum Aestivum ◽  
Resistance Genes ◽  
Biochemical Markers ◽  
Distal Part ◽  
Triticum Aestivum L ◽  
Wheat Genome ◽  
Addition Line ◽  
Wheat Line ◽  
Aegilops Ventricosa ◽  
Aegilops Uniaristata

An Aegilops ventricosa Tausch (2n = 28, DvDvNvNv) subtelocentric chromosome added to wheat (Triticum aestivum L.) in a disomic addition line was found to carry the genes for resistance Yr17, Lr37, Sr38, and Cre5 already transferred onto chromosome 2AS of the wheat line VPM1. Previous works demonstrated that this Ae. ventricosa chromosome is translocated with respect to the standard wheat genome. The present investigations showed that this chromosome pre-existed in Ae. ventricosa and contains only chromatin specific to the N genome. Using biochemical markers and suitable cytogenetic materials including the monoisosomic addition line for the translocated long arm (6NvL–2NvS), its structure was defined as being 6NvSdel.6NvL–2NvS. It consists of a segment of the short arm 2Nv, containing the resistance genes, attached to a group 6 chromosome lacking a distal part of its short arm. The 2 re arrangements could already be present in Aegilops uniaristata Vis. (2n = 14, NN), the source of the Nv genome of Ae. ventricosa.Key words: wheat, Aegilops ventricosa, GISH, translocation, isochromosome.

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