scholarly journals Heme Oxygenase-1 Inhibition Sensitizes Human Prostate Cancer Cells towards Glucose Deprivation and Metformin-Mediated Cell Death

2019 ◽  
Vol 20 (10) ◽  
pp. 2593 ◽  
Author(s):  
Marco Raffaele ◽  
Valeria Pittalà ◽  
Veronica Zingales ◽  
Ignazio Barbagallo ◽  
Loredana Salerno ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Death ◽  
Cell Viability ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Heme Oxygenase ◽  
Glucose Concentration ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells

High levels of heme oxygenase (HO)-1 have been frequently reported in different human cancers, playing a major role in drug resistance and regulation of cancer cell redox homeostasis. Metformin (MET), a drug widely used for type 2 diabetes, has recently gained interest for treating several cancers. Recent studies indicated that the anti-proliferative effects of metformin in cancer cells are highly dependent on glucose concentration. The present work was directed to determine whether use of a specific inhibitor of HO-1 activity, alone or in combination with metformin, affected metastatic prostate cancer cell viability under different concentrations of glucose. MTT assay and the xCELLigence system were used to evaluate cell viability and cell proliferation in DU145 human prostate cancer cells. Cell apoptosis and reactive oxygen species were analyzed by flow cytometry. The activity of HO-1 was inhibited using a selective imidazole-based inhibitor; genes associated with antioxidant systems and cell death were evaluated by qRT-PCR. Our study demonstrates that metformin suppressed prostate cancer growth in vitro and increased oxidative stress. Disrupting the antioxidant HO-1 activity, especially under low glucose concentration, could be an attractive approach to potentiate metformin antineoplastic effects and could provide a biochemical basis for developing HO-1-targeting drugs against solid tumors.

scholarly journals NF-κB activation enhances cell death by antimitotic drugs in human prostate cancer cells

2010 ◽  
Vol 9 (1) ◽  
pp. 182 ◽  
Author(s):  
Ricardo Parrondo ◽  
Alicia Pozas ◽  
Teresita Reiner ◽  
Priyamvada Rai ◽  
Carlos Perez-Stable
Keyword(s):  
Prostate Cancer ◽  
Cell Death ◽  
Cancer Cells ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Antimitotic Drugs

scholarly journals Endoplasmic reticulum stress, autophagic and apoptotic cell death, and immune activation by a natural triterpenoid in human prostate cancer cells

2018 ◽  
Vol 120 (4) ◽  
pp. 6264-6276 ◽  
Author(s):  
Benjamin M. Johnson ◽  
Faisal F. Y. Radwan ◽  
Azim Hossain ◽  
Bently P. Doonan ◽  
Jessica D. Hathaway‐Schrader ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Endoplasmic Reticulum ◽  
Cell Death ◽  
Endoplasmic Reticulum Stress ◽  
Cancer Cells ◽  
Immune Activation ◽  
Apoptotic Cell ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells

Apoptosis and Clonogenic Cell Death in PC3 Human Prostate Cancer Cells after Treatment with Gamma Radiation and Suramin

1997 ◽  
Vol 148 (2) ◽  
pp. 105 ◽  
Author(s):  
Sanjeewani T. Palayoor ◽  
Edward A. Bump ◽  
Beverly A. Teicher ◽  
C. Norman Coleman
Keyword(s):  
Prostate Cancer ◽  
Cell Death ◽  
Gamma Radiation ◽  
Cancer Cells ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Clonogenic Cell

The mechanism of protriptyline-induced Ca2+movement and non-Ca2+-triggered cell death in PC3 human prostate cancer cells

2015 ◽  
Vol 35 (5) ◽  
pp. 429-434 ◽  
Author(s):  
Hong-Tai Chang ◽  
Chiang-Ting Chou ◽  
Chia-Cheng Yu ◽  
Jeng-Yu Tsai ◽  
Te-Kung Sun ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Death ◽  
Cancer Cells ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells

scholarly journals URG11 Regulates Prostate Cancer Cell Proliferation, Migration, and Invasion

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Bin Pan ◽  
Yunlin Ye ◽  
Haiping Liu ◽  
Jianli Zhen ◽  
Hongmei Zhou ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Prostate Cancer Cell ◽  
Human Prostate ◽  
Migration And Invasion ◽  
Epithelial Cell Line ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Colon Cancers

Upregulated gene 11 (URG11), a new gene upregulated by hepatitis B virus X protein, is involved in the development and progression of several tumors, including liver, stomach, lung, and colon cancers. However, the role of URG11 in prostate cancer remains yet to be elucidated. By determined expression in human prostate cancer tissues, URG11 was found significantly upregulated and positively correlated with the severity of prostate cancer, compared with that in benign prostatic hyperplasia tissues. Further, the mRNA and protein levels of URG11 were significantly upregulated in human prostate cancer cell lines (DU145, PC3, and LNCaP), compared with human prostate epithelial cell line (RWPE-1). Moreover, by the application of siRNA against URG11, the proliferation, migration, and invasion of prostate cancer cells were markedly inhibited. Genetic knockdown of URG11 also induced cell cycle arrest at G1/S phase, induced apoptosis, and decreased the expression level of β-catenin in prostate cancer cells. Overexpression of URG11 promoted the expression of β-catenin, the growth, the migration, and invasion ability of prostate cancer cells. Taken together, this study reveals that URG11 is critical for the proliferation, migration, and invasion in prostate cancer cells, providing the evidence of URG11 to be a novel potential therapeutic target of prostate cancer.

Cytotoxicity of obacunone and obacunone glucoside in human prostate cancer cells involves Akt-mediated programmed cell death

Toxicology ◽  
2015 ◽  
Vol 329 ◽  
pp. 88-97 ◽  
Author(s):  
Kotamballi N. Chidambara Murthy ◽  
Guddadarangavvanahally K. Jayaprakasha ◽  
Bhimanagouda S Patil
Keyword(s):  
Prostate Cancer ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Cancer Cells ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells

Neutrophil-Secreted Proteinase 3 Mediates Metastasis of Prostate Cancer Cells Expressing RAGE to the Bone Marrow

Blood ◽  
2016 ◽  
Vol 128 (22) ◽  
pp. 1025-1025 ◽  
Author(s):  
Mikhail Kolonin ◽  
Anna Sergeeva ◽  
Daniela Staquicini ◽  
Jeffrey J. Molldrem ◽  
Renata Pasqualini ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Bone Marrow ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Prostate Cancer Cell ◽  
Human Prostate ◽  
Proteinase 3 ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells ◽  
Cell Surfaces

Abstract Human prostate cancer non-randomly metastasizes to the bone marrow, but the biological basis for such site-specific tropism remains largely unresolved. Differential expression of molecules in the bone marrow microenvironment "niche" has recently been proposed to play a role. In previous work, combinatorial selection of random peptide libraries in end-of-life cancer patients has revealed an unexpected interaction between the receptor for advanced glycation end products (RAGE), a molecule expressed on malignant cell surfaces in advanced prostate cancer, and proteinase 3 (PR3), a serine protease abundantly present on neutrophils and promyelocytes within the bone marrow microenvironment. Because RAGE is selectively overexpressed in prostate cancer bone metastases, we hypothesize here that its specific binding to PR3 might mediate homing of prostate cancer cells to the bone marrow. We demonstrate that PR3 non-proteolytically binds to RAGE on prostate cancer cell surfaces and thereby promotes tumor cell activation and motility. We also show that the downstream signal transduction cascade triggered by RAGE/PR3 binding involves p44/42 (Erk1/2) and JNK1 phosphorylation. Finally, we use a mouse model of experimental metastasis to demonstrate that RAGE protein expression on human prostate cancer cells promotes their homing to bone marrow within a short time frame. These results validate a functional protein interaction between RAGE and PR3 and uncover a mechanism through which neutrophils mediate prostate cancer cell metastasis to the skeleton. Disclosures Sergeeva: Astellas: Patents & Royalties. Molldrem:Astellas Pharma: Patents & Royalties.

Phytoestrogen modulation of bone-related cytokines and its impact on cell viability in human prostate cancer cells

Life Sciences ◽  
2009 ◽  
Vol 85 (11-12) ◽  
pp. 421-430 ◽  
Author(s):  
V. Alonso ◽  
F.C. Pérez-Martínez ◽  
F.J. Calahorra ◽  
P. Esbrit
Keyword(s):  
Prostate Cancer ◽  
Cell Viability ◽  
Cancer Cells ◽  
Human Prostate ◽  
Human Prostate Cancer ◽  
Prostate Cancer Cells
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