scholarly journals Molecular Aspects of Drug-Induced Gingival Overgrowth: An In Vitro Study on Amlodipine and Gingival Fibroblasts

2019 ◽  
Vol 20 (8) ◽  
pp. 2047 ◽  
Author(s):  
Dorina Lauritano ◽  
Alberta Lucchese ◽  
Dario Di Stasio ◽  
Fedora Della Vella ◽  
Francesca Cura ◽  
...  
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
In Vitro Study ◽  
Gingival Fibroblast ◽  
Gingival Hyperplasia ◽  
Drug Induced ◽  
Gingival Overgrowth ◽  
Molecular Aspects ◽  
Gene Expression Levels

Gingival overgrowth is a serious side effect that accompanies the use of amlodipine. Several conflicting theories have been proposed to explain the fibroblast’s function in gingival overgrowth. To determine whether amlodipine alters the fibrotic response, we investigated its effects on treated gingival fibroblast gene expression as compared with untreated cells. Materials and Methods: Fibroblasts from ATCC® Cell Lines were incubated with amlodipine. The gene expression levels of 12 genes belonging to the “Extracellular Matrix and Adhesion Molecules” pathway was investigated in treated fibroblasts cell culture, as compared with untreated cells, by real time PCR. Results: Most of the significant genes were up-regulated. (CTNND2, COL4A1, ITGA2, ITGA7, MMP10, MMP11, MMP12, MMP26) except for COL7A1, LAMB1, MMP8, and MMP16, which were down-regulated. Conclusion: These results seem to demonstrate that amlodipine has an effect on the extracellular matrix of gingival fibroblast. In the future, it would be interesting to understand the possible effect of the drug on fibroblasts of patients with amlodipine-induced gingival hyperplasia.

scholarly journals Drug-induced gingival hyperplasia: An in vitro study using amlodipine and human gingival fibroblasts

2019 ◽  
Vol 33 ◽  
pp. 205873841982774 ◽  
Author(s):  
Dorina Lauritano ◽  
Marcella Martinelli ◽  
Alessandro Baj ◽  
Giada Beltramini ◽  
Valentina Candotto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inflammatory Responses ◽  
Human Gingival Fibroblasts ◽  
Gingival Tissue ◽  
Gingival Fibroblast ◽  
Gingival Hyperplasia ◽  
Gingival Fibroblasts ◽  
Drug Induced ◽  
Gingival Overgrowth

Gingival overgrowth is a serious side effect that accompanies the use of amlodipine. Several conflicting theories have been proposed to explain the fibroblast’s function in gingival overgrowth. To determine whether amlodipine alters the inflammatory responses, we investigated its effects on gingival fibroblast gene expression as compared with untreated cells. Fragments of gingival tissue of healthy volunteers (11 years old boy, 68 years old woman, and 20 years old men) were collected during operation. Gene expression of 29 genes was investigated in gingival fibroblast cell culture treated with amlodipine, compared with untreated cells. Among the studied genes, only 15 ( CCL1, CCL2D, CCL5, CCL8, CXCL5, CXCL10, CCR1, CCR10, IL1A, IL1B, IL5, IL7, IL8, SPP1, and TNFSF10) were significantly deregulated. In particular, the most evident overexpressed genes in treated cells were CCR10 and IL1A. These results seem to indicate a possible role of amlodipine in the inflammatory response of treated human gingival fibroblasts.

scholarly journals Connective Tissue Metabolism and Gingival Overgrowth

2004 ◽  
Vol 15 (3) ◽  
pp. 165-175 ◽  
Author(s):  
P. C. Trackman ◽  
A. Kantarci
Keyword(s):  
Extracellular Matrix ◽  
Connective Tissue ◽  
Oral Hygiene ◽  
Tissue Specificity ◽  
Gingival Fibroblast ◽  
Drug Induced ◽  
Gingival Overgrowth ◽  
Connective Tissue Metabolism ◽  
Matrix Metabolism ◽  
Systemic Health

Gingival overgrowth occurs mainly as a result of certain anti-seizure, immunosuppressive, or antihypertensive drug therapies. Excess gingival tissues impede oral function and are disfiguring. Effective oral hygiene is compromised in the presence of gingival overgrowth, and it is now recognized that this may have negative implications for the systemic health of affected patients. Recent studies indicate that cytokine balances are abnormal in drug-induced forms of gingival overgrowth. Data supporting molecular and cellular characteristics that distinguish different forms of gingival overgrowth are summarized, and aspects of gingival fibroblast extracellular matrix metabolism that are unique to gingival tissues and cells are reviewed. Abnormal cytokine balances derived principally from lymphocytes and macrophages, and unique aspects of gingival extracellular matrix metabolism, are elements of a working model presented to facilitate our gaining a better understanding of mechanisms and of the tissue specificity of gingival overgrowth.

scholarly journals Drug-Induced Gingival Overgrowth: A Pilot Study on the Effect of Diphenylhydantoin and Gabapentin on Human Gingival Fibroblasts

2020 ◽  
Vol 17 (21) ◽  
pp. 8229
Author(s):  
Dorina Lauritano ◽  
Giulia Moreo ◽  
Luisa Limongelli ◽  
Elena Tregambi ◽  
Annalisa Palmieri ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Direct Action ◽  
Human Fibroblasts ◽  
Matrix Metalloproteases ◽  
Channel Blockers ◽  
Gingival Hyperplasia ◽  
Drug Induced ◽  
Gingival Overgrowth ◽  
Matrix Deposition ◽  
Extracellular Matrix Deposition

Introduction. The administration of several classes of drugs can lead to the onset of gingival overgrowth: anticonvulsants, immunosuppressants, and calcium channel blockers. Among the anticonvulsants, the main drug associated with gingival overgrowth is diphenylhydantoin. Materials and Methods. In this study, we compared the effects of diphenylhydantoin and gabapentin on 57 genes belonging to the “Extracellular Matrix and Adhesion Molecule” pathway, present in human fibroblasts of healthy volunteers. Results. Both molecules induce the same gene expression profile in fibroblasts as well as a significant upregulation of genes involved in extracellular matrix deposition like COL4A1, ITGA7, and LAMB3. The two treatments also induced a significant downregulation of genes involved in the expression of extracellular matrix metalloproteases like MMP11, MMP15, MMP16, MMP24, and transmembrane receptor ITGB4. Conclusions. Data recorded in our study confirmed the hypothesis of a direct action of these drugs at the periodontium level, inducing an increase in matrix production, a reduction in its degradation, and consequently resulting in gingival hyperplasia.

scholarly journals Biology of Drug-Induced Gingival Hyperplasia: In Vitro Study of the Effect of Nifedipine on Human Fibroblasts

2021 ◽  
Vol 11 (7) ◽  
pp. 3287
Author(s):  
Dorina Lauritano ◽  
Giulia Moreo ◽  
Fedora Della Vella ◽  
Annalisa Palmieri ◽  
Francesco Carinci ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Real Time ◽  
Human Fibroblasts ◽  
Control Group ◽  
Gingival Hyperplasia ◽  
Drug Induced ◽  
Gingival Overgrowth ◽  
Drug Concentrations ◽  
Gingival Enlargement ◽  
E Cadherin

Background: It has been proven that the antihypertensive agent nifedipine can cause gingival overgrowth as a side effect. The aim of this study was to analyze the effects of pharmacological treatment with nifedipine on human gingival fibroblasts activity, investigating the possible pathogenetic mechanisms that lead to the onset of gingival enlargement. Methods: The expression profile of 57 genes belonging to the “Extracellular Matrix and Adhesion Molecules” pathway, fibroblasts’ viability at different drug concentrations, and E-cadherin levels in treated fibroblasts were assessed using real-time Polymerase Chain Reaction, PrestoBlue™ cell viability test, and an enzyme-linked immunoassay (ELISA), respectively. Results: Metalloproteinase 24 and 8 (MMP24, MMP8) showed significant upregulation in treated cells with respect to the control group, and cell adhesion gene CDH1 (E-cadherin) levels were recorded as increased in treated fibroblasts using both real-time PCR and ELISA. Downregulation was observed for transmembrane receptors ITGA6 and ITGB4, the basement membrane constituent LAMA1 and LAMB1, and the extracellular matrix protease MMP11, MMP16, and MMP26. Conclusions: The obtained data suggested that the pathogenesis of nifedipine-induced gingival overgrowth is characterized by an excessive accumulation of collagen due to the inhibition of collagen intracellular and extracellular degradation pathways.

scholarly journals VARIOUS MANIFESTATIONS OF HYPERPLASIA OF PERIODONTAL TISSUES IN CHILDREN

2013 ◽  
Vol 9 (3) ◽  
pp. 56-62
Author(s):  
Т. Закиров ◽  
T. Zakirov ◽  
Е. Бимбас ◽  
E. Bimbas ◽  
Т. Стати ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Oral Hygiene ◽  
Conservative Therapy ◽  
Genetic Disorder ◽  
Gingival Hyperplasia ◽  
Complex Treatment ◽  
Connective Tissues ◽  
Drug Induced ◽  
Gingival Overgrowth ◽  
Periodontal Tissues

There are many reasons for gingival hyperplasia in  children. Mostly, proper oral hygiene is sufficient to achieve normal healthy gingiva. In some situations, however, gingival hyperplasia is drug induced or can be a manifestation of a genetic disorder. In the latter, it may exist as an isolated abnormality or as part of a syndrome. Gingival overgrowth is characterized by the accumulation of extracellular matrix in gingival connective  tissues, particularly collagenous components with various degrees of inflammation. The complex treatment of gingival overgrowth can include conservative therapy and traditional or laser gingivectomy

Effect of cyclosporin A on human gingival fibroblast collagen turnover in relation to the development of gingival overgrowth: an in vitro study

2004 ◽  
Vol 58 (4) ◽  
pp. 231-238 ◽  
Author(s):  
Nicoletta Gagliano ◽  
Claudia Moscheni ◽  
Claudia Dellavia ◽  
Carlo Torri ◽  
Giordano Stabellini ◽  
...  
Keyword(s):  
Cyclosporin A ◽  
In Vitro Study ◽  
Vitro Study ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Collagen Turnover ◽  
Gingival Overgrowth

scholarly journals Role of Cyclosporine in Gingival Hyperplasia: An In Vitro Study on Gingival Fibroblasts

2020 ◽  
Vol 21 (2) ◽  
pp. 595 ◽  
Author(s):  
Dorina Lauritano ◽  
Annalisa Palmieri ◽  
Alberta Lucchese ◽  
Dario Di Stasio ◽  
Giulia Moreo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Extracellular Matrix ◽  
Cyclosporine A ◽  
Matrix Metalloproteases ◽  
Gingival Hyperplasia ◽  
Gingival Fibroblasts ◽  
Down Regulation ◽  
Gingival Overgrowth ◽  
Expression Levels

Background: Gingival hyperplasia could occur after the administration of cyclosporine A. Up to 90% of the patients submitted to immunosuppressant drugs have been reported to suffer from this side effect. The role of fibroblasts in gingival hyperplasia has been widely discussed by literature, showing contrasting results. In order to demonstrate the effect of cyclosporine A on the extracellular matrix component of fibroblasts, we investigated the gene expression profile of human fibroblasts after cyclosporine A administration. Materials and methods: Primary gingival fibroblasts were stimulated with 1000 ng/mL cyclosporine A solution for 16 h. Gene expression levels of 57 genes belonging to the “Extracellular Matrix and Adhesion Molecules” pathway were analyzed using real-time PCR in treated cells, compared to untreated cells used as control. Results: Expression levels of different genes were significantly de-regulated. The gene CDH1, which codes for the cell adhesion protein E-cadherin, showed up-regulation. Almost all the extracellular matrix metalloproteases showed down-regulation (MMP8, MMP11, MMP15, MMP16, MMP24, MMP26). The administration of cyclosporine A was followed by down-regulation of other genes: COL7A1, the transmembrane receptors ITGB2 and ITGB4, and the basement membrane constituents LAMA2 and LAMB1. Conclusion: Data collected demonstrate that cyclosporine inhibits the secretion of matrix proteases, contributing to the accumulation of extracellular matrix components in the gingival connective tissue, causing gingival overgrowth. Patients affected by gingival overgrowth caused by cyclosporine A need to be further investigated in order to determine the role of this drug on fibroblasts.

Inhibition of Cyclosporin A-Induced Gingival Overgrowth by Azithromycin Through Phagocytosis: An In Vivo and In Vitro Study

2004 ◽  
Vol 75 (3) ◽  
pp. 380-387 ◽  
Author(s):  
Jeong-Won Paik ◽  
Chang-Sung Kim ◽  
Kyoo-Sung Cho ◽  
Jung-Kiu Chai ◽  
Chong-Kwan Kim ◽  
...  
Keyword(s):  
Cyclosporin A ◽  
In Vitro Study ◽  
Vitro Study ◽  
Gingival Overgrowth

An in-vitro study investigating the effect of air-abrasion bioactive glasses on dental adhesion, cytotoxicity and odontogenic gene expression

2021 ◽  
Author(s):  
Gianrico Spagnuolo ◽  
Paula Maciel Pires ◽  
Anna Calarco ◽  
Gianfranco Peluso ◽  
Avijit Banerjee ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Vitro Study ◽  
Vitro Study ◽  
Air Abrasion ◽  
Bioactive Glasses
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