scholarly journals Influence of Cigarette Smoke Inhalation on an Autogenous Onlay Bone Graft Area in Rats with Estrogen Deficiency: A Histomorphometric and Immunohistochemistry Study

2019 ◽  
Vol 20 (8) ◽  
pp. 1854 ◽  
Author(s):  
Camilla Magnoni Moretto Nunes ◽  
Daniella Vicensotto Bernardo ◽  
Camila Lopes Ferreira ◽  
Mônica Fernandes Gomes ◽  
Andrea Carvalho De Marco ◽  
...  
Keyword(s):  
Bone Graft ◽  
Cigarette Smoke ◽  
Bone Grafting ◽  
Test Group ◽  
Parietal Bone ◽  
Female Rats ◽  
Smoke Inhalation ◽  
Autogenous Bone ◽  
Collagen Membrane ◽  
Graft Area

Purpose: The present study aimed to evaluate the influence of cigarette smoke inhalation on an autogenous onlay bone graft area, either covered with a collagen membrane or not, in healthy and estrogen-deficient rats through histomorphometry and immunohistochemistry. Materials and Methods: Sixty female rats (Wistar), weighing 250–300 g, were randomly divided and allocated into groups (either exposed to cigarette smoke inhalation or not, ovariectomized and SHAM). After 15 days, the test group underwent cigarette smoke inhalation. Sixty days after exposition, autogenous bone grafting was only performed on all right hemimandibles, and the left ones underwent autogenous onlay bone grafting with the collagen membrane (BioGide®). The graft was harvested from the parietal bone and attached to the animals’ jaws (right and left). They were euthanized at 21, 45, and 60 days after grafting. Histological measurements and immunohistochemical analyses were performed, and results were submitted to a statistical analysis. Results: The addition of a collagen membrane to the bone graft proved more efficient in preserving graft area if compared to the graft area without a collagen membrane and the one associated with cigarette smoke inhalation at 21 (p = 0.0381) and 60 days (p = 0.0192), respectively. Cigarette smoke inhalation combined with ovariectomy promoted a significant reduction of the autogenous graft area at 21 and 60 days. At 45 days, no statistically significant results were observed. In the immunohistochemical analysis, the ovariectomized and smoking subgroups, combined or not with collagen membrane, received moderate and intense immunolabeling at 21 days for Receptor Activator of Nuclear Factor Kappa-B Ligand (RANKL) (p = 0.0017 and p = 0.0381, respectively). For Osteoprotegerin (OPG), intense immunolabeling was observed in most subgroups under analysis at 60 days. Conclusion: Smoking inhalation promoted resorption on the autogenous onlay bone graft, mainly when associated with ovariectomy. Furthermore, when associated with the collagen membrane, a lower resorption rate was observed if compared to the absence of the membrane.

scholarly journals Comparison of Calcium Sulfate and Bovine Collagen Barriers for Alveolar Ridge Augmentation

2014 ◽  
Vol 4 (3) ◽  
pp. 129-138
Author(s):  
Robert Spears ◽  
Ibtisam Al-Hashimi ◽  
Eric S Solomon ◽  
David G Kerns ◽  
William W Hallmon ◽  
...  
Keyword(s):  
Bone Graft ◽  
Calcium Sulfate ◽  
Autogenous Bone ◽  
Collagen Membrane ◽  
Alveolar Ridge ◽  
Bone Augmentation ◽  
Alveolar Ridge Augmentation ◽  
Ridge Augmentation ◽  
Bovine Collagen ◽  
Bone Gain

ABSTRACT Calcium sulfate is a biologically compatible osteoconductive graft material that binds underlying bone graft and provide space maintenance. The purpose of this study was to evaluate calcium sulfate as a barrier compared to a collagen membrane for augmentation of a standardized surgically created ridge defect. For this purpose, bilateral extraction of mandibular premolars was performed on six foxhounds (Canis familiaris). Eight weeks later, a standardized osseous ridge defects (24 total) were created using a 6 mm trephine. The study was approved by the Institutional Animal Care and Use Committee (IACUC) at Baylor College of Dentistry. The osseous defects were allocated into three groups (8 defects each): group 1 received autogenous bone graft covered with collagen membrane (CM); group 2 received autogenous bone graft covered with calcium sulfate barrier (CS), and group 3 was used as control and did not receive bone augmentation and was used as control. The animals were sacrificed after 12 weeks following bone augmentation and sites were evaluated histologically for total ridge width, percentage of bone gain and cortical bone thickness. Results All sites exhibited bony fill within the defect. Analysis of variance did not reveal statistically significant difference in the mean total bone gain among CM, CS, and control groups (12.2,11.6, and 11.9) mm2, respectively, p = 0.875. Conclusion Calcium sulfate does not appear to improve bone regeneration in an osseous defect. How to cite this article Heaton ML, Kerns DG, Hallmon WW, Kessler HP, Spears R, Solomon ES, Al-Hashimi I. Comparison of Calcium Sulfate and Bovine Collagen Barriers for Alveolar Ridge Augmentation. J Contemp Dent 2014;4(3):129-138.

scholarly journals Biphasic Calcium Phosphate Sphere Graft Combined with a Double-Layer Non-Crosslinked Collagen Membrane Technique for Ridge Preservation: A Randomized Controlled Animal Study

Materials ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 18 ◽  
Author(s):  
Jungwon Lee ◽  
Young-Jun Lim ◽  
Bongju Kim ◽  
Ki-Tae Koo ◽  
Yong-Moo Lee
Keyword(s):  
Bone Graft ◽  
Double Layer ◽  
Single Layer ◽  
Test Group ◽  
Control Group ◽  
Collagen Membrane ◽  
Ridge Preservation ◽  
Graft Material ◽  
Membrane Technique ◽  
Layer Membrane

The purpose of this study was to compare the histologic and radiologic differences between single- and double-layer collagen membrane techniques in flapless ridge preservation. The mandibular fourth premolar and first molar of four beagle dogs were used in the experiment. Mesial roots of the teeth were extracted and root canal treatment was performed at the distal roots. Ridge preservation was performed at the extraction sites using synthetic bone graft material. A single layer (control group) or double layer (test group) of non-crosslinked collagen membrane was applied following bone graft application. Three months later, the animals were sacrificed and micro-computed tomography (micro-CT) and histomorphometric analyses were conducted. Nonparametric Mann–Whitney test was performed to compare between the control and test groups. The vertical difference between buccal and lingual crests of control and test groups was 1.28 ± 0.41 and 0.53 ± 0.37 mm, respectively (p = 0.026). The mineralized bone area in control and test groups was 31.48% ± 7.41% and 42.25% ± 9.73%, respectively (p = 0.041). Within the limit of this study, ridge preservation using the double-layer membrane technique showed a reduced buccal bone resorption and improved new-bone formation in the ridge compared to that using the single-layer membrane technique.

Effects of melatonin and vitamin C on cigarette smoke–induced damage in the kidney

2007 ◽  
Vol 23 (8) ◽  
pp. 479-485 ◽  
Author(s):  
E Ozan ◽  
MF Sonmez ◽  
S Ozan ◽  
N Colakoglu ◽  
S Yilmaz ◽  
...  
Keyword(s):  
Vitamin C ◽  
Cigarette Smoke ◽  
Catalase Activity ◽  
Structural Changes ◽  
Kidney Tissue ◽  
Female Rats ◽  
Smoke Inhalation ◽  
Protective Effects ◽  
Group I ◽  
Group Ii

This study was carried out to investigate smoke-induced structural and biochemical changes and protective effects of co-administered melatonin and vitamin C in the kidney. Twenty-four Wistar adult female rats were used in this study. Animals were divided into four groups. The first group rats were used as control. The second group of rats inhaled cigarette smoke. Smile smoke inhaling third and fourth group rats received melatonin and vitamin C, respectively. At the end of experimental study, kidney tissues and blood samples were taken under ether anesthesia. Tissues were prepared and examined by light microscopy. Malondialdehyde and glutathione levels and catalase activity were determined. By light microscopic observation, a decrease of Bowman space of some renal corpuscles, foamy-like tubules, dilatation and congestion of the peritubuler vessels, and atrophy of the some renal corpuscles were observed in group II. In groups III and IV melatonin and vitamin C relatively protected the kidney tissue against smoke intoxication. Biochemical examination showed that malondialdehyde and glutathione levels and catalase activity in group II were higher than in group I. Melatonin and vitamin C injection to group III and IV caused a decrease in malondialdehyde and glutathione levels. Catalase activity did not change in these groups. We have shown that cigarette smoke inhalation caused structural changes in the kidney. However, melatonin and vitamin C administration produced in some degree protection against smoke-induced damage.

scholarly journals Cigarette smoke inhalation influences bone healing of post-extraction tooth socket: a histometric study in rats

2012 ◽  
Vol 23 (3) ◽  
pp. 228-234 ◽  
Author(s):  
Ana Paula Oliveira Giorgetti ◽  
João Batista César Neto ◽  
Márcio Zaffalon Casati ◽  
Enílson Antonio Sallum ◽  
Francisco Humberto Nociti Júnior
Keyword(s):  
Bone Formation ◽  
Cigarette Smoke ◽  
Bone Healing ◽  
Healing Process ◽  
Test Group ◽  
Smoke Inhalation ◽  
Control Group ◽  
Mineralized Tissue ◽  
Male Wistar Rats ◽  
Socket Healing

The aim of this study was to evaluate, histometrically, the bone healing of the molar extraction socket just after cigarette smoke inhalation (CSI). Forty male Wistar rats were randomly assigned to a test group (animals exposed to CSI, starting 3 days before teeth extraction and maintained until sacrifice; n=20) and a control group (animals never exposed to CSI; n=20). Second mandibular molars were bilaterally extracted and the animals (n=5/group/period) were sacrificed at 3, 7, 10 and 14 days after surgery. Digital images were analyzed according to the following histometric parameters: osteoid tissue (OT), remaining area (RA), mineralized tissue (MT) and non-mineralized tissue (NMT) in the molar socket. Intergroup analysis showed no significant differences at day 3 (p>0.05) for all parameters. On the 7th day, CSI affected negatively (p<0.05) bone formation with respect to NMT and RA (MT: 36%, NMT: 53%, RA: 12%; and MT: 39%, NMT: 29%, RA: 32%, for the control and test groups, respectively). In contrast, no statistically significant differences (p>0.05) were found at days 10 and 14. It may be concluded that CSI may affect socket healing from the early events involved in the healing process, which may be critical for the amount and quality of new-bone formation in smokers.

Bioactive Glass Granules as Extender of Autogenous Bone Grafting in Cementless Intercalary Implant of the Canine Femur

2007 ◽  
Vol 96 (3) ◽  
pp. 243-251 ◽  
Author(s):  
P. Keränen ◽  
A. Itälä ◽  
J. Koort ◽  
I. Kohonen ◽  
M. Dalstra ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Bone Graft ◽  
Bone Grafting ◽  
Paired Comparison ◽  
Autogenous Bone Graft ◽  
Autogenous Bone ◽  
Allogeneic Bone ◽  
Implant Surface ◽  
Autogenous Bone Grafting ◽  
Torsional Testing

Background and Aims: Ceramic bone graft substitutes have a potential to be used as replacement of allogeneic bone grafting and, under optimal distribution of particle size, they may even provide mechanical support. The current study examined the efficacy of bioactive glass granules as an extender of autogenous bone grafting in a segmental bone replacement model of the canine femur. Material and Method: A 16 mm long segment of the femur shaft was bilaterally replaced with an intercalary titanium implant in eight animals. The implant had cementless grooved proximal and distal stems. In one leg, the peri-implant space was packed with composite graft consisting of a mixture of bioactive glass granules and autogenous bone graft in proportion of 50:50. In the opposite leg, the peri-implant space was treated with autogenous bone graft alone. After surgery, unlimited functional loading was allowed. The outcome was evaluated at three months. Results: Eight out of sixteen autografted implants and seven out of sixteen composite-grafted implants were radiographically incorporated and clinically stable at three months. In the paired comparison, the proximal components of composite-grafted implants showed lower maximum load under torsional testing (p=0.068), less new bone in the longitudinal grooves of the stems (p=0.036) and lower affinity of new bone to implant surface (p=0.046). The distal components of the two sides showed a similar trend for less new bone in the grooves and lower bone affinity of new bone in the distal composite-grafted components. Conclusions: The current study suggests that supplementation of periprosthetic bone graft with bioactive ceramic particles may not help to promote healing of cementless implants under high dynamic loading conditions.

scholarly journals Conservative Technique Using Oral Dressing Material for Wound Dehiscence after Ridge Augmentation: A Technical Report with a Case Series

2021 ◽  
Vol 11 (13) ◽  
pp. 6115
Author(s):  
Jeong-Kui Ku ◽  
Yeong Kon Jeong ◽  
Yong-Suk Choi ◽  
Taeyeong Kim ◽  
In-Woo Cho ◽  
...  
Keyword(s):  
Conservative Treatment ◽  
Bone Graft ◽  
Case Series ◽  
Wound Dehiscence ◽  
Autogenous Bone ◽  
Collagen Membrane ◽  
Graft Material ◽  
Titanium Mesh ◽  
Ridge Augmentation ◽  
Secondary Healing

Wound dehiscence is the most frequent complication after ridge augmentation and causes postoperative infection, inadequate bone healing, or graft failure. In the oral cavity, conservative treatment for dehiscence is difficult to maintain until secondary healing occurs because of its normal flora, dynamic masticatory muscle movement, and humid environment. This paper reports an effective conservative method using an oral wound dressing material with an omnivec splint and presents three wound dehiscence cases: (1) autogenous tooth bone graft material with a collagen membrane, with dehiscence occurring at postoperative 5 days. (2) Autogenous bone graft covering titanium mesh, with dehiscence occurring at postoperative three weeks. The mesh was removed after 10 weeks with histologic analysis. (3) Autogenous bone and autogenous tooth bone graft covering a titanium mesh, with dehiscence occurring at postoperative 1 week. The exposed titanium mesh was maintained for 6 months after the graft. All cases achieved secondary healing and acceptable outcomes for a dental implant by conservative treatment without infection after the dehiscence after ridge augmentation.

Efficacy of Guided Bone Regeneration Using Composite Bone Graft and Resorbable Collagen Membrane in Seibert's Class I Ridge Defects: Radiological Evaluation

2013 ◽  
Vol 39 (4) ◽  
pp. 455-462 ◽  
Author(s):  
Saravanan Pushparajan ◽  
Ramakrishnan Thiagarajan ◽  
Ambalavanan Namasi ◽  
Pamela Emmadi ◽  
Harshini Saravanan
Keyword(s):  
Bone Graft ◽  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
Class I ◽  
Autogenous Bone ◽  
Collagen Membrane ◽  
Bovine Bone ◽  
Radiological Evaluation ◽  
Bone Width ◽  
Composite Bone

The purpose of the study was to evaluate radiologically the efficacy of guided bone regeneration using composite bone graft (autogenous bone graft and anorganic bovine bone graft [Bio-Oss]) along with resorbable collagen membrane (BioMend Extend) in the augmentation of Seibert's class I ridge defects in maxilla. Bone width was evaluated using computerized tomography at day 0 and at day 180 at 2 mm, 4 mm, and 6 mm from the crest. There was a statistically significant increase in bone width between day 0 and day 180 at 2 mm, 4 mm, and 6 mm from the crest. The results of the study demonstrated an increase in bone width of Seibert's class I ridge defects in the maxilla of the study patients.

Articular Talar Injuries in Athletes

2007 ◽  
Vol 35 (10) ◽  
pp. 1680-1687 ◽  
Author(s):  
Amol Saxena ◽  
Colin Eakin
Keyword(s):  
Bone Graft ◽  
Bone Grafting ◽  
Treatment Plan ◽  
Case Series ◽  
Autogenous Bone ◽  
Osteochondral Lesions ◽  
Return To Sports ◽  
Talar Dome ◽  
High Demand ◽  
Return To Activity

Background The treatment options of talar osteochondral lesions are numerous. Although studies show these treatments have been used with varying success, the ability to return to activity (RTA), including sports after treatment of talar dome injuries, have not been well documented. Hypothesis A treatment plan that uses microfracture for Hepple stage 2 through 4 lesions and autogenous bone grafting for Hepple stage 5 lesions for athletes with articular lesions of the talus will produce a high rate of return to athletic activity. Study Design Case series; Level of evidence, 4. Methods Preoperative and postoperative American Orthopaedic Foot and Ankle Society (AOFAS) scores and RTA were assessed prospectively 2 to 8 years after surgery in high-demand (athletic) patients with articular injuries to the talar dome treated according to the above protocol over a 6-year period. Results There were 26 microfracture procedures and 20 bone grafts to the talus. The AOFAS scores for both microfracture (preoperative, 54.6; postoperative, 94.4) and bone graft (preoperative, 46.1; postoperative, 93.4) patients improved significantly. The RTA for the entire group was 17.0 ± 5.3 weeks; for those undergoing microfracture, RTA was 15.1 ± 4.0 weeks; and for bone grafting, it was 19.6 ± 5.9 weeks. The RTA for the bone graft group was significantly slower than that of the microfracture group. Anterolateral lesions had significantly faster RTA and higher postoperative scores compared with other lesion locations. Arthroscopically treated lesions had similar postoperative AOFAS scores to those who had arthrotomy and did not have significantly faster RTA. Forty-four (96%) “excellent/good” AOFAS scores were achieved overall for talar lesions, with the same percentage of return to sport. Conclusions Talar bone grafting required a longer time to return to activity than microfracture in high-demand patients, but both groups had similar postoperative AOFAS scores. When applied to appropriate lesions, both techniques allow athletic patients to return to sports.

Sinus Augmentation With Platelet-Rich Fibrin in Combination With Bovine Bone Graft Versus Bovine Bone Graft in Combination With Collagen Membrane

2015 ◽  
Vol 41 (5) ◽  
pp. 586-595 ◽  
Author(s):  
Nilufer Bolukbasi ◽  
Selim Ersanlı ◽  
Nurullah Keklikoglu ◽  
Cansu Basegmez ◽  
Tayfun Ozdemir
Keyword(s):  
Bone Graft ◽  
Test Group ◽  
Control Group ◽  
Collagen Membrane ◽  
Bovine Bone ◽  
Graft Material ◽  
Sinus Augmentation ◽  
Platelet Rich Fibrin ◽  
Bone Graft Material ◽  
Sinus Floor

The purpose of this study was to compare the efficacy between the use of bovine bone graft material and platelet-rich fibrin (PRF) mixture (test group) and bovine bone graft material and collagen membrane combination (control group) in 2-stage maxillary sinus augmentation. According to specific inclusion/exclusion criteria, patients treated between 2008 and 2012 were selected. Panoramic radiographs were used for radiologic assessments. To evaluate the relationship between sinus-graft height and each implant, the bone level (BL) was divided by implant length (IL). To evaluate the change in the height of grafted sinus, the grafted sinus floor above the lowest part of the original sinus height (GSH) was divided by the original sinus height (OSH). Samples taken during implant surgery were used for histologic and histomorphometric analyses. Twenty-five patients, 32 augmentation surgeries, and 66 one-stage implants were included in the study. No implant loss or complication was observed in either group. There were no statistical differences according to new bone formation (P = .61) and biomaterial remnant (P = .87). During the evaluation period, the test group showed statistically less change in the BL/IL ratio (P = .022). The difference of GSH/OSH ratio was found to be insignificant between groups (P = .093). It was observed that the grafted sinus covering the implant apex and sinus floor was above the original sinus height in both groups. It may be concluded from this study that both combinations can be successfully used for sinus augmentation. Further studies evaluating different graft materials and PRF combinations in the early phases of healing would be beneficial.

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