scholarly journals Low-Luminance Blue Light-Enhanced Phototoxicity in A2E-Laden RPE Cell Cultures and Rats

2019 ◽  
Vol 20 (7) ◽  
pp. 1799 ◽  
Author(s):  
Lin ◽  
Wu ◽  
Huang ◽  
Chow ◽  
Hsiao ◽  
...  
Keyword(s):  
Retinal Degeneration ◽  
Blue Light ◽  
Synergistic Effects ◽  
Light Emitting Diode ◽  
Retinal Pigment ◽  
Light Emitting ◽  
Rpe Cells ◽  
Low Luminance ◽  
Angiogenic Genes

N-retinylidene-N-retinylethanolamine (A2E) and other bisretinoids are components of lipofuscin and accumulate in retinal pigment epithelial (RPE) cells—these adducts are recognized in the pathogenesis of retinal degeneration. Further, blue light-emitting diode (LED) light (BLL)-induced retinal toxicity plays an important role in retinal degeneration. Here, we demonstrate that low-luminance BLL enhances phototoxicity in A2E-laden RPE cells and rats. RPE cells were subjected to synthetic A2E, and the effects of BLL on activation of apoptotic biomarkers were examined by measuring the levels of cleaved caspase-3. BLL modulates the protein expression of zonula-occludens 1 (ZO-1) and paracellular permeability in A2E-laden RPE cells. Early inflammatory and angiogenic genes were also screened after short-term BLL exposure. In this study, we developed a rat model for A2E treatment with or without BLL exposure for 21 days. BLL exposure caused fundus damage, decreased total retinal thickness, and caused neuron transduction injury in the retina, which were consistent with the in vitro data. We suggest that the synergistic effects of BLL and A2E accumulation in the retina increase the risk of retinal degeneration. These outcomes help elucidate the associations between BLL/A2E and angiogenic/apoptotic mechanisms, as well as furthering therapeutic strategies.

scholarly journals In vitro and in vivo Efficacy of New Blue Light Emitting Diode Phototherapy Compared to Conventional Halogen Quartz Phototherapy for Neonatal Jaundice

2005 ◽  
Vol 20 (1) ◽  
pp. 61 ◽  
Author(s):  
Yun Sil Chang ◽  
Jong Hee Hwang ◽  
Hyuk Nam Kwon ◽  
Chang Won Choi ◽  
Sun Young Ko ◽  
...  
Keyword(s):  
Blue Light ◽  
Neonatal Jaundice ◽  
Light Emitting Diode ◽  
In Vivo Efficacy ◽  
Light Emitting

Antimicrobial Activity of an Implantable Wireless Blue Light-Emitting Diode Against Root Canal Biofilm In Vitro

2020 ◽  
Vol 38 (11) ◽  
pp. 694-702
Author(s):  
Ludan Zhang ◽  
Yamin Li ◽  
Qian Zhang ◽  
Ning Du ◽  
Xuefen Li ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Blue Light ◽  
Root Canal ◽  
Light Emitting Diode ◽  
Light Emitting

Conditioned media from blue light-emitting diode–exposed fibroblasts have an anti-inflammatory effect in vitro

2020 ◽  
Vol 36 (1) ◽  
pp. 99-109
Author(s):  
Phil-Sun Oh ◽  
Eun-Mi Kim ◽  
SeokTae Lim ◽  
Myung-Hee Sohn ◽  
Hwan-Jeong Jeong
Keyword(s):  
Blue Light ◽  
Light Emitting Diode ◽  
Conditioned Media ◽  
Light Emitting ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Tauroursodeoxycholic Acid Protects Retinal Pigment Epithelial Cells from Oxidative Injury and Endoplasmic Reticulum Stress In Vitro

Biomedicines ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 367
Author(s):  
Reem Hasaballah Alhasani ◽  
Mohammad Almarhoun ◽  
Xinzhi Zhou ◽  
James Reilly ◽  
Steven Patterson ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Er Stress ◽  
Retinal Degeneration ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Tauroursodeoxycholic Acid ◽  
Antioxidant Genes ◽  
Rpe Cells ◽  
Pigment Epithelial

Retinal degeneration is characterized by the dysfunction of retinal cells. Oxidative and endoplasmic reticulum (ER) stress play an important role in the pathogenesis and progression of retinal degeneration. Tauroursodeoxycholic acid (TUDCA) has been demonstrated to have protective effects in in vitro and in vivo retinal degeneration models. To fully understand the molecular mechanisms of TUDCA’s protection, we first treated human retinal pigment epithelial (RPE) cells, ARPE-19, with H2O2 or H2O2 plus TUDCA for 24 h. RPE cells co-exposed to TUDCA had higher cell viability and lower cell death rate compared to cells exposed to H2O2 alone. TUDCA significantly increased antioxidant capacity in H2O2-treated RPE cells by decreasing the generation of reactive oxygen species (ROS) and Malondialdehyde (MDA), upregulating the expression of antioxidant genes, and increasing the generation of glutathione (GSH). TUDCA also inhibited inflammation in H2O2-challenged RPE cells by decreasing the expression of proinflammatory cytokines. Furthermore, TUDCA suppressed thapsigargin-induced ER stress in RPE cells, as demonstrated by decreased the expression of CCAAT-enhancer-binding protein homologous protein (CHOP) and apoptosis. Our present study suggests that TUDCA can protect RPE cells against oxidative damage, inflammation, and ER stress and may benefit patients with retinal degeneration.

scholarly journals Blue Light Induces Down-Regulation of Aquaporin 1, 3, and 9 in Human Keratinocytes

Cells ◽  
2018 ◽  
Vol 7 (11) ◽  
pp. 197 ◽  
Author(s):  
Rosanna Avola ◽  
Adriana Graziano ◽  
Giovanna Pannuzzo ◽  
Venera Cardile
Keyword(s):  
Blue Light ◽  
Light Emitting Diode ◽  
Human Keratinocytes ◽  
Skin Aging ◽  
Nuclear Antigen ◽  
Down Regulation ◽  
Light Emitting ◽  
Proliferation And Differentiation ◽  
Vitro Model

The development in digital screen technology has exponentially increased in the last decades, and many of today’s electronic devices use light-emitting diode (LED) technology producing very strong blue light (BL) waves. Long-term exposure at LED-BL seems to have an implication in the dehydration of the epidermis, in the alterations of shape and number of the keratinocytes, and in the aging of the skin. Aquaporins (AQPs) are water membrane channels that permeate both water and glycerol and play an important role in the hydration of epidermis, as well as in proliferation and differentiation of keratinocytes. Thus, we have hypothesized that AQPs could be involved in the aging of the skin exposed to LED-BL. Therefore, we have examined the expression of AQPs in human keratinocytes exposed to LED-BL at dose of 45 J/cm2, used as an in vitro model to produce the general features of photo aging of the skin. The aim was to verify if LED-BL induces changes of the basal levels of AQPs. The keratinocytes exposure to LED-BL produced an increase of reactive oxygen species (ROS), an activation of 8-hydroxy-2’-deoxyguanosine (8-OHdG), an alteration of proliferating cell nuclear antigen (PCNA), and a down-regulation of AQP1, 3 and 9. These findings are preliminary evidences that may be used as starting points for further investigations about the mechanistic involvement of AQP1, 3, and 9 in LED-BL-induced skin aging.

Functional and morphological evaluations of blue light-emitting diode-induced retinal degeneration in mice

2015 ◽  
Vol 357 ◽  
pp. e180 ◽  
Author(s):  
I. Kim ◽  
G.H. Kim ◽  
S.S. Paik ◽  
H.I. Kim ◽  
M.Y. Lee ◽  
...  
Keyword(s):  
Retinal Degeneration ◽  
Blue Light ◽  
Light Emitting Diode ◽  
Light Emitting

scholarly journals In Vitro Bactericidal Effects of 625, 525, and 425 nm Wavelength (Red, Green, and Blue) Light-Emitting Diode Irradiation

2013 ◽  
Vol 31 (11) ◽  
pp. 554-562 ◽  
Author(s):  
SangWoo Kim ◽  
JiSun Kim ◽  
WonBong Lim ◽  
SangMi Jeon ◽  
OkSu Kim ◽  
...  
Keyword(s):  
Blue Light ◽  
Light Emitting Diode ◽  
Light Emitting ◽  
Bactericidal Effects
Sign in / Sign up

Export Citation Format

Share Document