scholarly journals Identification of Candidate Ergosterol-Responsive Proteins Associated with the Plasma Membrane of Arabidopsis thaliana

2019 ◽  
Vol 20 (6) ◽  
pp. 1302 ◽  
Author(s):  
Thembisile Khoza ◽  
Ian Dubery ◽  
Lizelle Piater
Keyword(s):  
Arabidopsis Thaliana ◽  
Plasma Membrane ◽  
Crop Production ◽  
Plant Responses ◽  
Receptor Kinase ◽  
Sustainable Food ◽  
Molecular Pattern ◽  
Major Sterol ◽  
Related Proteins ◽  
The Impact

The impact of fungal diseases on crop production negatively reflects on sustainable food production and overall economic health. Ergosterol is the major sterol component in fungal membranes and regarded as a general elicitor or microbe-associated molecular pattern (MAMP) molecule. Although plant responses to ergosterol have been reported, the perception mechanism is still unknown. Here, Arabidopsis thaliana protein fractions were used to identify those differentially regulated following ergosterol treatment; additionally, they were subjected to affinity-based chromatography enrichment strategies to capture and categorize ergosterol-interacting candidate proteins using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Mature plants were treated with 250 nM ergosterol over a 24 h period, and plasma membrane-associated fractions were isolated. In addition, ergosterol was immobilized on two different affinity-based systems to capture interacting proteins/complexes. This resulted in the identification of defense-related proteins such as chitin elicitor receptor kinase (CERK), non-race specific disease resistance/harpin-induced (NDR1/HIN1)-like protein, Ras-related proteins, aquaporins, remorin protein, leucine-rich repeat (LRR)- receptor like kinases (RLKs), G-type lectin S-receptor-like serine/threonine-protein kinase (GsSRK), and glycosylphosphatidylinositol (GPI)-anchored protein. Furthermore, the results elucidated unknown signaling responses to this MAMP, including endocytosis, and other similarities to those previously reported for bacterial flagellin, lipopolysaccharides, and fungal chitin.

scholarly journals The bacterial quorum sensing signal DSF hijacks Arabidopsis thaliana sterol biosynthesis to suppress plant innate immunity

2020 ◽  
Vol 3 (10) ◽  
pp. e202000720
Author(s):  
Tuan Minh Tran ◽  
Zhiming Ma ◽  
Alexander Triebl ◽  
Sangeeta Nath ◽  
Yingying Cheng ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Arabidopsis Thaliana ◽  
Plasma Membrane ◽  
Quorum Sensing ◽  
Xanthomonas Campestris ◽  
Pathogenic Bacteria ◽  
Specific Effect ◽  
Molecular Pattern ◽  
Pathogen Associated Molecular Pattern ◽  
Bacterial Quorum

Quorum sensing (QS) is a recognized phenomenon that is crucial for regulating population-related behaviors in bacteria. However, the direct specific effect of QS molecules on host biology is largely understudied. In this work, we show that the QS molecule DSF (cis-11-methyl-dodecenoic acid) produced by Xanthomonas campestris pv. campestris can suppress pathogen-associated molecular pattern–triggered immunity (PTI) in Arabidopsis thaliana, mediated by flagellin-induced activation of flagellin receptor FLS2. The DSF-mediated attenuation of innate immunity results from the alteration of FLS2 nanoclusters and endocytic internalization of plasma membrane FLS2. DSF altered the lipid profile of Arabidopsis, with a particular increase in the phytosterol species, which impairs the general endocytosis pathway mediated by clathrin and FLS2 nano-clustering on the plasma membrane. The DSF effect on receptor dynamics and host immune responses could be entirely reversed by sterol removal. Together, our results highlighted the importance of sterol homeostasis to plasma membrane organization and demonstrate a novel mechanism by which pathogenic bacteria use their communicating molecule to manipulate pathogen-associated molecular pattern–triggered host immunity.

scholarly journals LRR-extensins of vegetative tissues are a functionally conserved family of RALF1 receptors interacting with the receptor kinase FERONIA

2019 ◽  
Author(s):  
Aline Herger ◽  
Shibu Gupta ◽  
Gabor Kadler ◽  
Christina Maria Franck ◽  
Aurélien Boisson-Dernier ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Plasma Membrane ◽  
Cell Growth ◽  
Mechanical Stability ◽  
Root Hairs ◽  
Cell Wall Integrity ◽  
Receptor Kinase ◽  
Growth Processes ◽  
Evolutionary Diversification

AbstractPlant cell growth requires the coordinated expansion of the protoplast and the cell wall that confers mechanical stability to the cell. An elaborate system of cell wall integrity sensors monitors cell wall structures and conveys information on cell wall composition and growth factors to the cell. LRR-extensins (LRXs) are cell wall-attached extracellular regulators of cell wall formation and high-affinity binding sites for RALF (rapid alkalinization factor) peptide hormones that trigger diverse physiological processes related to cell growth. RALF peptides are also perceived by receptors at the plasma membrane and LRX4 of Arabidopsis thaliana has been shown to also interact with one of these receptors, FERONIA (FER). Here, we demonstrate that several LRXs, including the main LRX protein of root hairs, LRX1, interact with FER and RALF1 to coordinate growth processes. Membrane association of LRXs correlate with binding to FER, indicating that LRXs represent a physical link between intra- and extracellular compartments via interaction with membrane-localized proteins. Finally, despite evolutionary diversification of the LRR domains of various LRX proteins, many of them are functionally still overlapping, indicative of LRX proteins being central players in regulatory processes that are conserved in very different cell types.Author SummaryCell growth in plants requires the coordinated enlargement of the cell and the surrounding cell wall, which is ascertained by an elaborate system of cell wall integrity sensors, proteins involved in the exchange of information between the cell and the cell wall. In Arabidopsis thaliana, LRR-extensins (LRXs) are localized in the cell wall and are binding RALF peptides, hormones that regulate cell growth-related processes. LRX4 also binds the plasma membrane-localized receptor kinase FERONIA (FER), establishing a link between the cell and the cell wall. It is not clear, however, whether the different LRXs of Arabidopsis have similar functions and how they interact with their binding partners. Here, we demonstrate that interaction with FER and RALFs requires the LRR domain of LRXs and several but not all LRXs can bind these proteins. This explains the observation that mutations in several of the LRXs induce phenotypes comparable to a fer mutant, establishing that LRX-FER interaction is important for proper cell growth. Some LRXs, however, appear to influence cell growth processes in different ways, which remain to be identified.

scholarly journals CONCEPT AND RATIONALE OF EVOLUTIONARY BARLEY BREEDING UNDER CLIMATE CHANGE IN JORDAN

2021 ◽  
Vol 9 (8) ◽  
pp. 150-167
Author(s):  
Nawal Al‐Hajaj ◽  
Omar Kafawin
Keyword(s):  
Climate Change ◽  
Plant Breeding ◽  
Crop Production ◽  
Food System ◽  
Smallholder Farmers ◽  
Breeding Strategy ◽  
Climate Change Scenarios ◽  
Individual Selection ◽  
Sustainable Food ◽  
The Impact

In this study, we reviewed the climate changing and the impact on crop production, and evolutionary breeding as adaptation key to crop resilience. The increasing climate change impact on the agriculture system has renewed interest to the broadest possible germplasm base for a resilient and sustainable food system. Heterogeneous populations developed through evolutionary plant breeding could be the ideal solution to reduce the effects of environment variability on cereal crop planted under low-input conditions.The study assessed the genetic basis of adaptation of a barley population which evolved in different rainfed locations and years in Jordan without any human selection as suggests model of plant breeding strategy to improve food security, nutrition, income and resilience of smallholder farmers in the dryland regions in the climate change scenarios. The study suggests that the breeder can shift the undesirable traits in evolutionary populations by practicing individual selection for specific adaptations, or individual selection from populations showing wide adaptations and high stability. On the other hand, the breeder can overcome the undesirable traits by keeping the highest variations within the population by seed sieving to remove small seed and plant mowing for tallest head.

Pengaruh Konversi Lahan terhadap Strategi Pemanfaatan Modal Nafkah Rumah Tangga Buruh Tani

2020 ◽  
Vol 4 (3) ◽  
pp. 407
Author(s):  
Hesti Prastiwi ◽  
Martua Sihaloho
Keyword(s):  
Qualitative Data ◽  
Rice Fields ◽  
Livelihood Strategies ◽  
Quantitative Approach ◽  
Survey Method ◽  
Land Conversion ◽  
Literature Study ◽  
Irrigated Rice ◽  
Sustainable Food ◽  
The Impact

Lahan merupakan salah satu sumber daya agraria yang sangat penting bagi petani dikarenakan lahan merupakan salah satu sumber untuk bertahan hidup. Sesuai dengan Undang-Undang Dasar Nomor 41 Tahun 2009 tentang Perlindungan Lahan Pertanian Pangan Berkelanjutan. Pembangunan yang terjadi di Indonesia semakin masif, menimbulkan konversi lahan bersifat permanen ketika lahan sawah beririgasi berubah menjadi kawasan pemukiman atau industri. Konversi lahan yang terjadi menyebabkan hilangnya beberapa atau seluruh modal nafkah yang ada. Perubahan modal nafkah ini akan mengakibatkan perubahan strategi nafkah petani. Penelitian ini bertujuan untuk menganalisis dampak konversi lahan terhadap strategi nafkah dan pengaruh pemanfaatan  modal nafkah  terhadap strategi nafkah rumah tangga. Lokasi penelitian ini yaitu Desa Pasirgaok, Kecamatan Rancabungur, Kabupaten Bogor. Metode yang digunakan untuk menggali fakta, data, dan informasi dalam penelitian adalah pendekatan kuantitatif melalui metode survei dengan kuesioner yang didukung oleh data kualitatif melalui wawancara dan studi literatur hingga diperoleh hasil bahwa dampak konversi lahan mempengaruhi modal dan strategi nafkah.Kata Kunci: buruh tani, konversi lahan, modal nafkah, strategi nafkah=====ABSTRACTLand is one of the most important agrarian resources for farmers because land is one source of livelihood survival. According to Constitution Number 41 Year 2009 on concerning The Protection of Sustainable Food Agriculture. The development that is taking place in Indonesia is increasingly massive leading to permanent land conversion when technical irrigated rice fields change into residential or industrial areas. The land conversion that occurs causes a loss of some or all existing livelihood asset. This change in livelihood asset will result in a change in farmers livelihood strategies. This study aims to analyze the influence of the impact of land conversion towards livelihood strategies and the influence of the utilization of livelihood asset towards the livelihood strategies households. The location of this research is Pasirgaok Village, Rancabungur District, Bogor Regency. The method used to extract facts, data, and information in the research is the quantitative approach through survey method with questionnaires supported by qualitative data through interview and literature study until it is obtained that the impact of land conversion towards landless farmer household’s assets and livelihood strategies.Keywords: land conversion, landless farmer, livelihood asset, livelihood strategies

A Macroeconomic Analysis of Agricultural Sector in Nigeria

2019 ◽  
Vol 5 (1) ◽  
pp. 18-25
Author(s):  
Isah Funtua Abubakar ◽  
Umar Bambale Ibrahim
Keyword(s):  
Value Chain ◽  
Crop Production ◽  
Agricultural Sector ◽  
Time Series Data ◽  
Tourism Industry ◽  
Series Data ◽  
Size Analysis ◽  
The Real ◽  
Real Gdp ◽  
The Impact

This paper attempts to study the Nigerian agriculture industry as a panacea to growth as well as an anchor to the diversification agenda of the present government. To do this, the time series data of the four agriculture subsectors of crop production, livestock, forestry and fishery were analysed as stimulus to the Real GDP from 1981-2016 in order to explicate the individual contributions of the subsectors to the RGDP in order to guide the policy thrust on diversification. Using the Johansen approach to cointegration, all the variables were found to be cointegrated. With the exception of the forestry subsector, all the three subsectors were seen to have impacted on the real GDP at varying degrees during the time under review. The crop production subsector has the highest impact, however, taking size-by-size analysis, the livestock subsector could be of much importance due to its ability to retain its value chain and high investment returns particularly in poultry. Therefore, it is recommended that, the government should intensify efforts to retain the value chain in the crop production subsector, in order to harness its potentials optimally through the encouragement of the establishment of agriculture cottage industries. Secondly, the livestock subsector is found to be the most rapidly growing and commercialized subsector. Therefore, it should be the prime subsector to hinge the diversification agenda naturally. Lastly, the tourism industry which is a source through which the impact of the subsector is channeled to the GDP should be developed, in order to improve the impact of such channel to GDP with the sole objective to resuscitate the forestry subsector.

Effect of Nanobionics in crop production- A Review

2020 ◽  
Vol 10 ◽  
Author(s):  
Vinayak Fasake ◽  
Nita Patil ◽  
Zoya Javed ◽  
Mansi Mishra ◽  
Gyan Tripathi ◽  
...  
Keyword(s):  
Crop Production ◽  
Seed Quality ◽  
Negative Impact ◽  
Potential Solution ◽  
New Era ◽  
Mineral Toxicity ◽  
The Impact ◽  
Favourable Situation ◽  
Unique Chemical ◽  
Macro Nutrients

: Nanobionics involves the improvement of plant or plant productivity using nanomaterials. Growth of a plant from a seed encompasses various factors which are directly or indirectly dependent upon the imbibition of micro and macro nutrients and vital elements from the soil. Since most of the nutrition is physiologically unavailable to the plants, it leads to mineral deficiencies in plant and mineral toxicity in soil. Either ways, it is not a favourable situation for the microcosom. The new era of nanotechnology offers a potential solution to the availability of the nutrients to the plants due to its unique chemical and physical properties of nanoparticles. Positive and negative impact of these nanoparticles on seed quality and plant growth varies according to the specific properties of nanoparticles. The present review is an attempt to summarize the impact of nanobionics in agriculture.

scholarly journals Characterization of Interactions between the Soybean Salt-Stress Responsive Membrane-Intrinsic Proteins GmPIP1 and GmPIP2

Agronomy ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1312
Author(s):  
Jia Liu ◽  
Weicong Qi ◽  
Haiying Lu ◽  
Hongbo Shao ◽  
Dayong Zhang
Keyword(s):  
Plasma Membrane ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Gene Expression Profiles ◽  
Early Gene ◽  
Plant Responses ◽  
Constitutive Overexpression ◽  
Important Trait

Salt tolerance is an important trait in soybean cultivation and breeding. Plant responses to salt stress include physiological and biochemical changes that affect the movement of water across the plasma membrane. Plasma membrane intrinsic proteins (PIPs) localize to the plasma membrane and regulate the water and solutes flow. In this study, quantitative real-time PCR and yeast two-hybridization were engaged to analyze the early gene expression profiles and interactions of a set of soybean PIPs (GmPIPs) in response to salt stress. A total of 20 GmPIPs-encoding genes had varied expression profiles after salt stress. Among them, 13 genes exhibited a downregulated expression pattern, including GmPIP1;6, the constitutive overexpression of which could improve soybean salt tolerance, and its close homologs GmPIP1;7 and 1;5. Three genes showed upregulated patterns, including the GmPIP1;6 close homolog GmPIP1;4, when four genes with earlier increased and then decreased expression patterns. GmPIP1;5 and GmPIP1;6 could both physically interact strongly with GmPIP2;2, GmPIP2;4, GmPIP2;6, GmPIP2;8, GmPIP2;9, GmPIP2;11, and GmPIP2;13. Definite interactions between GmPIP1;6 and GmPIP1;7 were detected and GmPIP2;9 performed homo-interaction. The interactions of GmPIP1;5 with GmPIP2;11 and 2;13, GmPIP1;6 with GmPIP2;9, 2;11 and GmPIP2;13, and GmPIP2;9 with itself were strengthened upon salt stress rather than osmotic stress. Taken together, we inferred that GmPIP1 type and GmPIP2 type could associate with each other to synergistically function in the plant cell; a salt-stress environment could promote part of their interactions. This result provided new clues to further understand the soybean PIP–isoform interactions, which lead to potentially functional homo- and heterotetramers for salt tolerance.

scholarly journals Improving Drought Tolerance in Mungbean (Vigna radiata L. Wilczek): Morpho-Physiological, Biochemical and Molecular Perspectives

Agronomy ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1534
Author(s):  
Chandra Mohan Singh ◽  
Poornima Singh ◽  
Chandrakant Tiwari ◽  
Shalini Purwar ◽  
Mukul Kumar ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Crop Production ◽  
Mitigation Strategies ◽  
Theoretical Research ◽  
Whole Genome Sequence ◽  
Complex Nature ◽  
Drought Tolerant ◽  
Breeding Programmes ◽  
The Impact

Drought stress is considered a severe threat to crop production. It adversely affects the morpho-physiological, biochemical and molecular functions of the plants, especially in short duration crops like mungbean. In the past few decades, significant progress has been made towards enhancing climate resilience in legumes through classical and next-generation breeding coupled with omics approaches. Various defence mechanisms have been reported as key players in crop adaptation to drought stress. Many researchers have identified potential donors, QTLs/genes and candidate genes associated to drought tolerance-related traits. However, cloning and exploitation of these loci/gene(s) in breeding programmes are still limited. To bridge the gap between theoretical research and practical breeding, we need to reveal the omics-assisted genetic variations associated with drought tolerance in mungbean to tackle this stress. Furthermore, the use of wild relatives in breeding programmes for drought tolerance is also limited and needs to be focused. Even after six years of decoding the whole genome sequence of mungbean, the genome-wide characterization and expression of various gene families and transcriptional factors are still lacking. Due to the complex nature of drought tolerance, it also requires integrating high throughput multi-omics approaches to increase breeding efficiency and genomic selection for rapid genetic gains to develop drought-tolerant mungbean cultivars. This review highlights the impact of drought stress on mungbean and mitigation strategies for breeding high-yielding drought-tolerant mungbean varieties through classical and modern omics technologies.

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