scholarly journals The Inescapable Effects of Ribosomes on In-Cell NMR Spectroscopy and the Implications for Regulation of Biological Activity

2019 ◽  
Vol 20 (6) ◽  
pp. 1297 ◽  
Author(s):  
David Burz ◽  
Leonard Breindel ◽  
Alexander Shekhtman
Keyword(s):  
Biological Activity ◽  
Nmr Spectroscopy ◽  
Quantum Coherence ◽  
Kinetic Properties ◽  
Multiple Quantum ◽  
Transverse Relaxation ◽  
Molecular Weights ◽  
Heteronuclear Multiple Quantum Coherence ◽  
Kinetic Activity

The effects of RNA on in-cell NMR spectroscopy and ribosomes on the kinetic activity of several metabolic enzymes are reviewed. Quinary interactions between labelled target proteins and RNA broaden in-cell NMR spectra yielding apparent megadalton molecular weights in-cell. The in-cell spectra can be resolved by using cross relaxation-induced polarization transfer (CRINEPT), heteronuclear multiple quantum coherence (HMQC), transverse relaxation-optimized, NMR spectroscopy (TROSY). The effect is reproduced in vitro by using reconstituted total cellular RNA and purified ribosome preparations. Furthermore, ribosomal binding antibiotics alter protein quinary structure through protein-ribosome and protein-mRNA-ribosome interactions. The quinary interactions of Adenylate kinase, Thymidylate synthase and Dihydrofolate reductase alter kinetic properties of the enzymes. The results demonstrate that ribosomes may specifically contribute to the regulation of biological activity.

Heteronuclear Multiple-Quantum Coherence NMR Spectroscopy of Im-cytC

1995 ◽  
Vol 28 (4) ◽  
pp. 641-650
Author(s):  
Weiping Shao ◽  
Xiaoling Huang ◽  
Gaohua Liu ◽  
Houming Wu ◽  
Wenxia Tang
Keyword(s):  
Nmr Spectroscopy ◽  
Quantum Coherence ◽  
Multiple Quantum ◽  
Heteronuclear Multiple Quantum Coherence ◽  
Multiple Quantum Coherence

scholarly journals Trypanocidal and Leishmanicidal Activities of Sesquiterpene Lactones from Ambrosia tenuifolia Sprengel (Asteraceae)

2008 ◽  
Vol 52 (7) ◽  
pp. 2415-2419 ◽  
Author(s):  
Valeria P. Sülsen ◽  
Fernanda M. Frank ◽  
Silvia I. Cazorla ◽  
Claudia A. Anesini ◽  
Emilio L. Malchiodi ◽  
...  
Keyword(s):  
Quantum Coherence ◽  
Sesquiterpene Lactones ◽  
Multiple Quantum ◽  
Heteronuclear Multiple Quantum Coherence ◽  
Bioassay Guided Fractionation ◽  
In Vivo Testing ◽  
Electron Impact Mass ◽  
First Time

ABSTRACT Bioassay-guided fractionation of the organic extract of Ambrosia tenuifolia Sprengel (Asteraceae) led to the isolation of two bioactive sesquiterpene lactones with significant trypanocidal and leishmanicidal activities. By spectroscopic methods (1H- and 13C-nuclear magnetic resonance, distortionless enhancement by polarization transfer, correlated spectroscopy, heteronuclear multiple-quantum coherence, electron impact-mass spectrometry, and infrared spectroscopy), these compounds were identified as psilostachyin and peruvin. Both compounds showed a marked in vitro trypanocidal activity against Trypanosoma cruzi epimastigotes with 50% inhibitory concentration (IC50) values of less than 2 μg/ml. Psilostachyin exerted a significant in vitro activity against the trypomastigote forms of T. cruzi (IC50, 0.76 μg/ml) and was selected for in vivo testing. Psilostachyin-treated mice had a survival of 100% and lower parasitemia values than control mice. Both compounds were also tested on Leishmania sp. promastigotes: psilostachyin (IC50, 0.12 μg/ml) and peruvin (IC50, 0.39 μg/ml) exerted significant leishmanicidal activities. This is the first time that the trypanocidal and leishmanicidal activities of these compounds have been reported. The selectivity index (SI) was employed to evaluate the cytotoxic effect of lactones on T lymphocytes. Although the SIs of both compounds were high for T. cruzi epimastigotes, psilostachyin was more selective against trypomastigotes (SI, 33.8) while peruvin showed no specificity for this parasite. Both compounds presented high selectivity for Leishmania spp. The results shown herein suggest that psilostachyin and peruvin could be considered potential candidates for the development of new antiprotozoal agents against Chagas' disease and leishmaniasis.

Use of1H−15N Heteronuclear Multiple-Quantum Coherence NMR Spectroscopy To Study the Active Site of Aspartate Aminotransferase†

Biochemistry ◽  
1997 ◽  
Vol 36 (3) ◽  
pp. 615-625 ◽  
Author(s):  
Emilia T. Mollova ◽  
David E. Metzler ◽  
Agustin Kintanar ◽  
Hiroyuki Kagamiyama ◽  
Hideyuki Hayashi ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Active Site ◽  
Aspartate Aminotransferase ◽  
Quantum Coherence ◽  
Multiple Quantum ◽  
Heteronuclear Multiple Quantum Coherence ◽  
Multiple Quantum Coherence

NMR studies on Fraser fir Abies fraseri (Pursh) Poir. lignins

Holzforschung ◽  
2005 ◽  
Vol 59 (5) ◽  
pp. 488-496 ◽  
Author(s):  
Mikhail Yu. Balakshin ◽  
Ewellyn A. Capanema ◽  
Barry Goldfarb ◽  
John Frampton ◽  
John F. Kadla
Keyword(s):  
Quantum Coherence ◽  
Lignin Content ◽  
Lower Amount ◽  
Multiple Quantum ◽  
Normal Wood ◽  
Nmr Studies ◽  
Oh Groups ◽  
Fraser Fir ◽  
Heteronuclear Multiple Quantum Coherence ◽  
Balsam Woolly Adelgid

Abstract The composition of mature, juvenile uninfested and juvenile infested Fraser fir wood (Rotholz) and the structures of lignins isolated from these woods were elucidated to verify differences between juvenile and mature wood and the effect of balsam woolly adelgid (BWA) infestation. Milled wood lignin (MWL) isolated from mature, juvenile and Rotholz wood were comprehensively characterized using heteronuclear multiple quantum coherence (HMQC) and quantitative 13C NMR techniques. The Rotholz wood was found to have ∼13% higher lignin content and more than five-fold the amount of galactans than that of the uninfested wood. Rotholz lignin possesses higher amounts of p-hydroxyphenyl units and aliphatic OH groups and a lower amount of alkyl-O-alkyl linkages and dibenzodioxocin moieties. The degree of condensation of the Rotholz lignin was rather similar to that of normal wood. Only small differences in the structure of mature and juvenile wood components were found.

scholarly journals Correction: 19F multiple-quantum coherence NMR spectroscopy for probing protein–ligand interactions

RSC Advances ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 3503-3503
Author(s):  
Anna Zawadzka-Kazimierczuk ◽  
Mate Somlyay ◽  
Hanspeter Kaehlig ◽  
George Iakobson ◽  
Petr Beier ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Quantum Coherence ◽  
Multiple Quantum ◽  
Protein Ligand Interactions ◽  
Ligand Interactions ◽  
Multiple Quantum Coherence

Correction for ‘19F multiple-quantum coherence NMR spectroscopy for probing protein–ligand interactions’ by Anna Zawadzka-Kazimierczuk et al., RSC Adv., 2018, 8, 40687–40692.

scholarly journals Spatially Selective Heteronuclear Multiple-Quantum Coherence Spectroscopy for Biomolecular NMR Studies

ChemPhysChem ◽  
2014 ◽  
Vol 15 (9) ◽  
pp. 1872-1879 ◽  
Author(s):  
Bharathwaj Sathyamoorthy ◽  
David M. Parish ◽  
Gaetano T. Montelione ◽  
Rong Xiao ◽  
Thomas Szyperski
Keyword(s):  
Quantum Coherence ◽  
Multiple Quantum ◽  
Nmr Studies ◽  
Biomolecular Nmr ◽  
Heteronuclear Multiple Quantum Coherence ◽  
Coherence Spectroscopy ◽  
Multiple Quantum Coherence

scholarly journals Dependence of in vivo glutamine synthetase activity on ammonia concentration in rat brain studied by 1H - 15N heteronuclear multiple-quantum coherence-transfer NMR

1995 ◽  
Vol 311 (2) ◽  
pp. 681-688 ◽  
Author(s):  
K Kanamori ◽  
B D Ross ◽  
E L Kuo
Keyword(s):  
Quantum Coherence ◽  
Ammonia Concentration ◽  
Multiple Quantum ◽  
Blood Ammonia ◽  
Coherence Transfer ◽  
Heteronuclear Multiple Quantum Coherence ◽  
Glutamine Synthesis ◽  
Gaba Synthesis ◽  
Multiple Quantum Coherence

The dependence of the in vivo rate of glutamine synthesis on the substrate ammonia concentration was studied in rat brain by 1H-15N heteronuclear multiple-quantum coherence-transfer NMR in combination with biochemical techniques. In vivo rates were measured at various steady-state blood and brain ammonia concentrations within the ranges 0.4-0.55 mumol/g and 0.86-0.98 mumol/g respectively, after low-rate intravenous 15NH4+ infusion (isotope chase). The rate of glutamine synthesis at steady state was determined from the change in brain [5-15N]glutamine levels during isotope chase, observed selectively through the amide proton by NMR, and 15N enrichments of brain glutamine and of blood and brain ammonia measured byN gas chromatography-MS. The in vivo rate (v) was 3.3-4.5 mumol/h per g of brain at blood ammonia concentrations (s) of 0.40-0.55 mumol/g. A linear increase of 1/v with 1/s permitted estimation of the in vivo glutamine synthetase (GS) activity at a physiological blood ammonia concentration to be 0.4-2.1 mumol/h per g. The observed ammonia-dependence strongly suggests that, under physiological conditions, in vivo GS activity is kinetically limited by sub-optimal in situ concentrations of ammonia as well as glutamate and ATP. Comparison of the observed in vivo GS activity with the reported in vivo rates of glutaminase and of gamma-aminobutyrate (GABA) synthesis suggests that, under mildly hyperammonaemic conditions, glutamine is synthesized at a sufficiently high rate to serve as a precursor of GABA, but glutaminase-catalysed hydrolysis of glutamine is too slow to be the sole provider of glutamate used for GABA synthesis.

scholarly journals t1-Noise eliminated dipolar heteronuclear multiple-quantum coherence solid-state NMR spectroscopy

2020 ◽  
Vol 22 (36) ◽  
pp. 20815-20828 ◽  
Author(s):  
Amrit Venkatesh ◽  
Xuechen Luan ◽  
Frédéric A. Perras ◽  
Ivan Hung ◽  
Wenyu Huang ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Quantum Correlation ◽  
Quantum Coherence ◽  
Pulse Sequences ◽  
Multiple Quantum ◽  
Heteronuclear Correlation ◽  
Heteronuclear Multiple Quantum Coherence ◽  
Multiple Quantum Coherence ◽  
Nuclear Magnetic Resonance Pulse

t1-Noise eliminated (TONE) heteronuclear multiple quantum correlation (HMQC) solid-state nuclear magnetic resonance pulse sequences improve the sensitivity of 2D 1H{X} heteronuclear correlation experiments with X = 17O, 25Mg, 27Al and 35Cl.

Sign in / Sign up

Export Citation Format

Share Document