scholarly journals Map-Based Cloning and Functional Analysis of YE1 in Rice, Which Is Involved in Light-Dependent Chlorophyll Biogenesis and Photoperiodic Flowering Pathway

2019 ◽  
Vol 20 (3) ◽  
pp. 758 ◽  
Author(s):  
Youlin Peng ◽  
Ting Zou ◽  
Lamei Li ◽  
Shiwen Tang ◽  
Qiao Li ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Early Flowering ◽  
Yellow Leaf ◽  
Rhythmic Expression ◽  
Rice Mutant ◽  
Photoperiodic Flowering ◽  
Cloning Method ◽  
Flowering Pathway ◽  
Photoperiod Sensitive ◽  
Chlorophyll Biogenesis

Light is one of the most important environmental factors that affect many aspects of plant growth, including chlorophyll (Chl) synthesis and flowering time. Here, we identified a rice mutant, yellow leaf and early flowering (ye1), and characterized the gene YE1 by using a map-based cloning method. YE1 encodes a heme oxygenase, which is localized to the chloroplasts. YE1 is expressed in various green tissues, especially in leaves, with a diurnal-rhythmic expression pattern, and its transcripts is also induced by light during leaf-greening. The mutant displays decreased Chl contents with less and disorderly thylakoid lamellar layers in chloroplasts, which reduced the photosynthesis rate. The early flowering phenotype of ye1 was not photoperiod-sensitive. Furthermore, the expression levels of Chl biosynthetic genes were downregulated in ye1 seedlings during de-etiolation responses to light. We also found that rhythmic expression patterns of genes involved in photoperiodic flowering were altered in the mutant. Based on these results, we infer that YE1 plays an important role in light-dependent Chl biogenesis as well as photoperiodic flowering pathway in rice.

EF8 is involved in photoperiodic flowering pathway and chlorophyll biogenesis in rice

2014 ◽  
Vol 33 (12) ◽  
pp. 2003-2014 ◽  
Author(s):  
Zhiming Feng ◽  
Long Zhang ◽  
Chunyan Yang ◽  
Tao Wu ◽  
Jia Lv ◽  
...  
Keyword(s):  
Photoperiodic Flowering ◽  
Flowering Pathway ◽  
Chlorophyll Biogenesis

scholarly journals Genome-Wide Analysis of CCT Transcript Factors to Identify Genes Contributing to Photoperiodic Flowering in Oryza rufipogon

2021 ◽  
Vol 12 ◽  
Author(s):  
Xin Peng ◽  
Win Tun ◽  
Shuang-feng Dai ◽  
Jia-yue Li ◽  
Qun-jie Zhang ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Functional Characterization ◽  
Oryza Rufipogon ◽  
Developmental Expression ◽  
Cultivated Rice ◽  
Functional Roles ◽  
Rhythmic Expression ◽  
Photoperiodic Flowering ◽  
In The Wild ◽  
Functional Alleles

Photoperiod sensitivity is a dominant determinant for the phase transition in cereal crops. CCT (CONSTANS, CO-like, and TOC1) transcription factors (TFs) are involved in many physiological functions including the regulation of the photoperiodic flowering. However, the functional roles of CCT TFs have not been elucidated in the wild progenitors of crops. In this study, we identified 41 CCT TFs, including 19 CMF, 17 COL, and five PRR TFs in Oryza rufipogon, the presumed wild ancestor of Asian cultivated rice. There are thirty-eight orthologous CCT genes in Oryza sativa, of which ten pairs of duplicated CCT TFs are shared with O. rufipogon. We investigated daily expression patterns, showing that 36 OrCCT genes exhibited circadian rhythmic expression. A total of thirteen OrCCT genes were identified as putative flowering suppressors in O. rufipogon based on rhythmic and developmental expression patterns and transgenic phenotypes. We propose that OrCCT08, OrCCT24, and OrCCT26 are the strong functional alleles of rice DTH2, Ghd7, and OsPRR37, respectively. The SD treatment at 80 DAG stimulated flowering of the LD-grown O. rufipogon plants. Our results further showed that the nine OrCCT genes were significantly downregulated under the treatment. Our findings would provide valuable information for the construction of photoperiodic flowering regulatory network and functional characterization of the CCT TFs in both O. rufipogon and O. sativa.

scholarly journals GhLUX1 and GhELF3 Are Two Components of the Circadian Clock That Regulate Flowering Time of Gossypium hirsutum

2021 ◽  
Vol 12 ◽  
Author(s):  
Pengbo Hao ◽  
Aimin Wu ◽  
Pengyun Chen ◽  
Hantao Wang ◽  
Liang Ma ◽  
...  
Keyword(s):  
Circadian Clock ◽  
Flowering Time ◽  
Clock Genes ◽  
Early Flowering ◽  
Rhythmic Expression ◽  
Photoperiodic Flowering ◽  
Early Maturing ◽  
Circadian Clock Genes ◽  
Delayed Flowering ◽  
Physicochemical And Structural Properties

Photoperiod is an important external factor that regulates flowering time, the core mechanism of which lies in the circadian clock-controlled expression of FLOWERING LOCUS T (FT) and its upstream regulators. However, the roles of the circadian clock in regulating cotton flowering time are largely unknown. In this study, we cloned two circadian clock genes in cotton, GhLUX1 and GhELF3. The physicochemical and structural properties of their putative proteins could satisfy the prerequisites for the interaction between them, which was proved by yeast two-hybrid (Y2H) and Bimolecular Fluorescent Complimentary (BiFC) assays. Phylogenetic analysis of LUXs and ELF3s indicated that the origin of LUXs was earlier than that of ELF3s, but ELF3s were more divergent and might perform more diverse functions. GhLUX1, GhELF3, GhCOL1, and GhFT exhibited rhythmic expression and were differentially expressed in the early flowering and late-flowering cotton varieties under different photoperiod conditions. Both overexpression of GhLUX1 and overexpression of GhELF3 in Arabidopsis delayed flowering probably by changing the oscillation phases and amplitudes of the key genes in the photoperiodic flowering pathway. Both silencing of GhLUX1 and silencing of GhELF3 in cotton increased the expression of GhCOL1 and GhFT and resulted in early flowering. In summary, the circadian clock genes were involved in regulating cotton flowering time and could be the candidate targets for breeding early maturing cotton varieties.

Stability of the Synechococcus elongatus PCC 7942 circadian clock under directed anti-phase expression of the kai genes

Microbiology ◽  
2005 ◽  
Vol 151 (8) ◽  
pp. 2605-2613 ◽  
Author(s):  
Jayna L. Ditty ◽  
Shannon R. Canales ◽  
Breanne E. Anderson ◽  
Stanly B. Williams ◽  
Susan S. Golden
Keyword(s):  
Circadian Clock ◽  
Expression Patterns ◽  
Phase Relationship ◽  
Synechococcus Elongatus ◽  
Cycle Period ◽  
Rhythmic Expression ◽  
Core Components ◽  
Synechococcus Elongatus Pcc 7942 ◽  
Time Required ◽  
Pcc 7942

The kaiA, kaiB and kaiC genes encode the core components of the cyanobacterial circadian clock in Synechococcus elongatus PCC 7942. Rhythmic expression patterns of kaiA and of the kaiBC operon normally peak in synchrony. In some mutants the relative timing of peaks (phase relationship) between these transcription units is altered, but circadian rhythms persist robustly. In this study, the importance of the transcriptional timing of kai genes was examined. Expressing either kaiA or kaiBC from a heterologous promoter whose peak expression occurs 12 h out of phase from the norm, and thus 12 h out of phase from the other kai locus, did not affect the time required for one cycle (period) or phase of the circadian rhythm, as measured by bioluminescence reporters. Furthermore, the data confirm that specific cis elements within the promoters of the kai genes are not necessary to sustain clock function.

Daily Rhythmic Expression Patterns ofClock1a,Bmal1, andPer1Genes in Retina and Hypothalamus of the Rainbow Trout,Oncorhynchus Mykiss

2011 ◽  
Vol 28 (5) ◽  
pp. 381-389 ◽  
Author(s):  
Marcos A. López Patiño ◽  
Arnau Rodríguez-Illamola ◽  
Marta Conde-Sieira ◽  
José L. Soengas ◽  
Jesús M. Míguez
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Expression Patterns ◽  
Rhythmic Expression ◽  
Rainbow Trout Oncorhynchus Mykiss

scholarly journals DNA methylation affects photoperiodic tuberization in potato (Solanum tuberosum L.) by mediating the expression of genes related to the photoperiod and GA pathways

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Yanjun Ai ◽  
Shenglin Jing ◽  
Zhengnan Cheng ◽  
Botao Song ◽  
Conghua Xie ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Solanum Tuberosum L ◽  
Tuber Initiation ◽  
Short Day ◽  
Photoperiodic Flowering ◽  
Differentially Methylated Genes ◽  
Wide Range ◽  
Methylation Inhibitor ◽  
Dna Methylation Inhibitor ◽  
Photoperiod Sensitive

AbstractOvercoming short-day-dependent tuberization to adapt to long-day conditions is critical for the widespread geographical success of potato. The genetic pathways of photoperiodic tuberization are similar to those of photoperiodic flowering. DNA methylation plays an important role in photoperiodic flowering. However, little is known about how DNA methylation affects photoperiodic tuberization in potato. Here, we verified the effect of a DNA methylation inhibitor on photoperiodic tuberization and compared the DNA methylation levels and differentially methylated genes (DMGs) in the photoperiodic tuberization process between photoperiod-sensitive and photoperiod-insensitive genotypes, aiming to dissect the role of DNA methylation in the photoperiodic tuberization of potato. We found that a DNA methylation inhibitor could promote tuber initiation in strict short-day genotypes. Whole-genome DNA methylation sequencing showed that the photoperiod-sensitive and photoperiod-insensitive genotypes had distinct DNA methylation modes in which few differentially methylated genes were shared. Transcriptome analysis confirmed that the DNA methylation inhibitor regulated the expression of the key genes involved in the photoperiod and GA pathways to promote tuber initiation in the photoperiod-sensitive genotype. Comparison of the DNA methylation levels and transcriptome levels identified 52 candidate genes regulated by DNA methylation that were predicted to be involved in photoperiodic tuberization. Our findings provide a new perspective for understanding the relationship between photoperiod-dependent and GA-regulated tuberization. Uncovering the epigenomic signatures of these pathways will greatly enhance potato breeding for adaptation to a wide range of environments.

Circadian clock regulates granulosa cell autophagy through NR1D1-mediated inhibition of ATG5

2021 ◽  
Author(s):  
Jing Zhang ◽  
Lijia Zhao ◽  
Yating Li ◽  
Hao Dong ◽  
Haisen Zhang ◽  
...  
Keyword(s):  
Circadian Clock ◽  
Clock Genes ◽  
Expression Patterns ◽  
Current Data ◽  
Orphan Receptor ◽  
Luciferase Reporter ◽  
Mobility Shift ◽  
Rhythmic Expression ◽  
Clock System ◽  
Circadian Clock Genes

Autophagy of granulosa cells (GCs) is involved in follicular atresia, which occurs repeatedly during the ovarian development cycle. Several circadian clock genes are rhythmically expressed in both rodent ovarian tissues and GCs. Nuclear receptor subfamily 1 group D member 1 (NR1D1), an important component of the circadian clock system, is involved in the autophagy process through the regulation of autophagy-related genes. However, there are no reports illustrating the role of the circadian clock system in mouse GC autophagy. In the present study, we found that core circadian clock genes (Bmal1, Per2, Nr1d1, and Dbp) and an autophagy-related gene (Atg5) exhibited rhythmic expression patterns across 24 h in mouse ovaries and primary GCs. Treatment with SR9009, an agonist of NR1D1, significantly reduced the expression of Bmal1, Per2, and Dbp in mouse GCs. ATG5 expression was significantly attenuated by SR9009 treatment in mouse GCs. Conversely, Nr1d1 knockdown increased ATG5 expression in mouse GCs. Decreased NR1D1 expression at both the mRNA and protein levels was detected in the ovaries of Bmal1-/- mice, along with elevated expression of ATG5. Dual-luciferase reporter assay and electrophoretic mobility shift assay showed that NR1D1 inhibited Atg5 transcription by binding to two putative retinoic acid-related orphan receptor response elements within the promoter. In addition, rapamycin-induced autophagy and ATG5 expression were partially reversed by SR9009 treatment in mouse GCs. Taken together, our current data demonstrated that the circadian clock regulates GC autophagy through NR1D1-mediated inhibition of ATG5 expression, and thus, plays a role in maintaining autophagy homeostasis in GCs.

Circadian regulation of the transcriptome in a complex polyploid crop

2021 ◽  
Author(s):  
Hannah Rees ◽  
Rachel Rusholme-Pilcher ◽  
Paul Bailey ◽  
Joshua Colmer ◽  
Benjamen White ◽  
...  
Keyword(s):  
Time Course ◽  
Expression Patterns ◽  
Wheat Breeding ◽  
Circadian Regulation ◽  
Biological Processes ◽  
Starch Metabolism ◽  
Resolution Time ◽  
Rhythmic Expression ◽  
Circadian Control ◽  
Selection For

AbstractThe circadian clock is a finely balanced time-keeping mechanism that coordinates programmes of gene expression. In polyploids, this regulation must be coordinated over multiple subgenomes. Here, we generate and analyse a high-resolution time-course dataset to investigate the circadian balance between sets of three homoeologous genes (triads) from hexaploid bread wheat. We find a large proportion of circadian triads exhibit unbalanced rhythmic expression patterns, with no specific subgenome favoured. In wheat, period lengths of rhythmic transcripts are found to be longer and have a higher level of variance than in other plant species. Biological processes under circadian control are largely conserved between wheat and Arabidopsis, however striking differences are seen in agriculturally critical processes such as starch metabolism. Together, this work highlights the ongoing selection for balance versus diversification in circadian homoeologs, and identifies clock-controlled pathways that might provide important targets for future wheat breeding.

Effects of Shading on the Senescence and Photosynthetic Physiology of the Early-Flowering Rice Mutant FTL10 at Noon

2019 ◽  
Vol 39 (2) ◽  
pp. 776-784
Author(s):  
Qilei Zhang ◽  
Junjie Zhai ◽  
Yanxia Wei ◽  
Lina Lu ◽  
Changlian Peng
Keyword(s):  
Early Flowering ◽  
Rice Mutant ◽  
Photosynthetic Physiology
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