scholarly journals Hyaluronic Acid/Bone Substitute Complex Implanted on Chick Embryo Chorioallantoic Membrane Induces Osteoblastic Differentiation and Angiogenesis, but not Inflammation

2018 ◽  
Vol 19 (12) ◽  
pp. 4119 ◽  
Author(s):  
Laura Cirligeriu ◽  
Anca Cimpean ◽  
Horia Calniceanu ◽  
Mircea Vladau ◽  
Simona Sarb ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Chorioallantoic Membrane ◽  
Bone Substitutes ◽  
Osteoblastic Differentiation ◽  
Mesenchymal Cells ◽  
Alveolar Ridge ◽  
Alveolar Ridge Augmentation ◽  
Ridge Augmentation ◽  
Molecular Events ◽  
Chick Embryo Chorioallantoic Membrane

Microscopic and molecular events related to alveolar ridge augmentation are less known because of the lack of experimental models and limited molecular markers used to evaluate this process. We propose here the chick embryo chorioallantoic membrane (CAM) as an in vivo model to study the interaction between CAM and bone substitutes (B) combined with hyaluronic acid (BH), saline solution (BHS and BS, respectively), or both, aiming to point out the microscopic and molecular events assessed by Runt-related transcription factor 2 (RUNX 2), osteonectin (SPARC), and Bone Morphogenic Protein 4 (BMP4). The BH complex induced osteoprogenitor and osteoblastic differentiation of CAM mesenchymal cells, certified by the RUNX2 +, BMP4 +, and SPARC + phenotypes capable of bone matrix synthesis and mineralization. A strong angiogenic response without inflammation was detected on microscopic specimens of the BH combination compared with an inflammatory induced angiogenesis for the BS and BHS combinations. A multilayered organization of the BH complex grafted on CAM was detected with a differential expression of RUNX2, BMP4, and SPARC. The BH complex induced CAM mesenchymal cells differentiation through osteoblastic lineage with a sustained angiogenic response not related with inflammation. Thus, bone granules resuspended in hyaluronic acid seem to be the best combination for a proper non-inflammatory response in alveolar ridge augmentation. The CAM model allows us to assess the early events of the bone substitutes–mesenchymal cells interaction related to osteoblastic differentiation, an important step in alveolar ridge augmentation.

scholarly journals Candidates Cell Sources to Regenerate Alveolar Bone from Oral Tissue

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Masahiro Nishimura ◽  
Kazuma Takase ◽  
Fumio Suehiro ◽  
Hiroshi Murata
Keyword(s):  
Bone Regeneration ◽  
Alveolar Bone ◽  
Mesenchymal Cells ◽  
Gingival Tissue ◽  
Rational Approach ◽  
Alveolar Ridge ◽  
Alveolar Ridge Augmentation ◽  
Differentiation Potential ◽  
Ridge Augmentation ◽  
Oral Site

Most of the cases of dental implant surgery, especially the bone defect extensively, are essential for alveolar ridge augmentation. As known as cell therapy exerts valuable effects on bone regeneration, numerous reports using various cells from body to regenerate bone have been published, including clinical reports. Mesenchymal cells that have osteogenic activity and have potential to be harvested from intra oral site might be a candidate cells to regenerate alveolar bone, even dentists have not been harvested the cells outside of mouth. This paper presents a summary of somatic cells in edentulous tissues which could subserve alveolar bone regeneration. The candidate tissues that might have differentiation potential as mesenchymal cells for bone regeneration are alveolar bone chip, bone marrow from alveolar bone, periosteal tissue, and gingival tissue. Understanding their phenotype consecutively will provide a rational approach for alveolar ridge augmentation.

Scanning electron microscopy of composite hydroxylapatite/plaster implants for bone reconstruction

1986 ◽  
Vol 44 ◽  
pp. 326-327
Author(s):  
J. S. Hanker ◽  
B. L. Giammara
Keyword(s):  
Tooth Extraction ◽  
Alveolar Ridge ◽  
Small Particles ◽  
Alveolar Ridge Augmentation ◽  
Sintered Ceramic ◽  
Ridge Augmentation ◽  
Fibrovascular Tissue ◽  
Infrabony Defects ◽  
Edentulous Patients ◽  
Scanning Electron

Nonresorbable sintered ceramic hydroxylapatite (HA) is widely employed for filling defects in jaw bone. The small particles used for alveolar ridge augmentation in edentulous patients or for infrabony defects due to periodontal disease tend to scatter when implanted using water or saline as the vehicle. Larger blocks of this material used for filling sockets after tooth extraction don't fit well. Studies in our laboratory where we compared bovine serum albumin, collagen and plaster of Paris as binders to prevent particle scatter during implantation suggested that plaster was most useful for this purpose. In addition to preventing scatter of the particles, plaster enables the formation of implants of any size and.shape either prior to or during surgery. Studies with the PATS reaction have indicated that plaster acts as a scaffold for the incorporation of HA particles into bone in areas where the implant contacts either host bone or periosteum. The shape and integrity of the implant is maintained by the plaster component until it is replaced over a period of days by fibrovascular tissue.

Alveolar ridge augmentation by osteoinductive materials in goats

1992 ◽  
Vol 100 (6) ◽  
pp. 361-365
Author(s):  
Else Marie Pinholt ◽  
Hans Reidar Haanaes ◽  
Magnus Roervik ◽  
Karl Donath ◽  
Gisle Bang
Keyword(s):  
Alveolar Ridge ◽  
Alveolar Ridge Augmentation ◽  
Ridge Augmentation ◽  
Osteoinductive Materials
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