scholarly journals Biocompatibility and Biological Efficiency of Inorganic Calcium Filled Bacterial Cellulose Based Hydrogel Scaffolds for Bone Bioengineering

2018 ◽  
Vol 19 (12) ◽  
pp. 3980 ◽  
Author(s):  
Probal Basu ◽  
Nabanita Saha ◽  
Radostina Alexandrova ◽  
Boyka Andonova-Lilova ◽  
Milena Georgieva ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bacterial Cellulose ◽  
Bone Tissue ◽  
Human Fibroblasts ◽  
Biological Efficiency ◽  
Hydrogel Scaffold ◽  
Hydrogel Scaffolds ◽  
Depth Analysis

The principal focus of this work is the in-depth analysis of the biological efficiency of inorganic calcium-filled bacterial cellulose (BC) based hydrogel scaffolds for their future use in bone tissue engineering/bioengineering. Inorganic calcium was filled in the form of calcium phosphate (β-tri calcium phosphate (β-TCP) and hydroxyapatite (HA)) and calcium carbonate (CaCO3). The additional calcium, CaCO3 was incorporated following in vitro bio-mineralization. Cell viability study was performed with the extracts of BC based hydrogel scaffolds: BC-PVP, BC-CMC; BC-PVP-β-TCP/HA, BC-CMC-β-TCP/HA and BC-PVP-β-TCP/HA-CaCO3, BC-CMC-β-TCP/HA-CaCO3; respectively. The biocompatibility study was performed with two different cell lines, i.e., human fibroblasts, Lep-3 and mouse bone explant cells. Each hydrogel scaffold has facilitated notable growth and proliferation in presence of these two cell types. Nevertheless, the percentage of DNA strand breaks was higher when cells were treated with BC-CMC based scaffolds i.e., BC-CMC-β-TCP/HA and BC-CMC-β-TCP/HA-CaCO3. On the other hand, the apoptosis of human fibroblasts, Lep-3 was insignificant in BC-PVP-β-TCP/HA. The scanning electron microscopy confirmed the efficient adhesion and growth of Lep-3 cells throughout the surface of BC-PVP and BC-PVP-β-TCP/HA. Hence, among all inorganic calcium filled hydrogel scaffolds, ‘BC-PVP-β-TCP/HA’ was recommended as an efficient tissue engineering scaffold which could facilitate the musculoskeletal (i.e., bone tissue) engineering/bioengineering.

scholarly journals Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering

2021 ◽  
Vol 12 ◽  
pp. 204173142110056
Author(s):  
Nupur Kohli ◽  
Vaibhav Sharma ◽  
Alodia Orera ◽  
Prasad Sawadkar ◽  
Nazanin Owji ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Chorioallantoic Membrane ◽  
Cam Assay ◽  
Clinical Model ◽  
Chick Chorioallantoic Membrane ◽  
Biomimetic Method ◽  
Alginate Scaffold

Due to the limitations of bone autografts, we aimed to develop new composite biomaterials with pro-angiogenic and osteogenic properties to be used as scaffolds in bone tissue engineering applications. We used a porous, cross-linked and slowly biodegradable fibrin/alginate scaffold originally developed in our laboratory for wound healing, throughout which deposits of calcium phosphate (CaP) were evenly incorporated using an established biomimetic method. Material characterisation revealed the porous nature and confirmed the deposition of CaP precursor phases throughout the scaffolds. MC3T3-E1 cells adhered to the scaffolds, proliferated, migrated and differentiated down the osteogenic pathway during the culture period. Chick chorioallantoic membrane (CAM) assay results showed that the scaffolds were pro-angiogenic and biocompatible. The work presented here gave useful insights into the potential of these pro-angiogenic and osteogenic scaffolds for bone tissue engineering and merits further research in a pre-clinical model prior to its clinical translation.

Preparation and characterization of nano biphasic calcium phosphate/poly-L-lactide composite scaffold

2016 ◽  
Vol 23 (1) ◽  
pp. 37-44 ◽  
Author(s):  
Weizhong Yang ◽  
Yong Yi ◽  
Yuan Ma ◽  
Li Zhang ◽  
Jianwen Gu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Cell Viability ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biphasic Calcium Phosphate ◽  
Composite Scaffold ◽  
Pore Wall ◽  
Tissue Engineering Scaffold ◽  
Plla Scaffold

AbstractNano biphasic calcium phosphate (BCP) particles were synthesized using the sol-gel method. As-prepared BCP particles were combined with poly-L-lactide (PLLA) to fabricate nano-BCP/PLLA composite scaffold through a series of processing steps containing solvent self-diffusion, hot-pressing, and particulate leaching. The composite had a suitable porous structure for bone tissue engineering scaffold. In comparison, micro-BCP/PLLA scaffold was studied as well. Nano-BCP particles were distributed homogeneously in the PLLA matrix, and much more tiny crystallites exposed on the surface of the pore wall. Due to the finer inorganic particle distribution in the PLLA phase and the larger area of the bioactive phase exposed in the pore wall surface, nano-BCP/PLLA scaffold had enhanced compressive strength, good bioactivity, and superior cell viability. A nonstoichiometric apatite layer could be rapidly formed on the surface of nano- BCP/PLLA when soaked in simulated body fluid. The MG-63 cell viability of nano-BCP/PLLA scaffold is significantly higher than that of micro-BCP/PLLA scaffold. Therefore, nano-BCP/PLLA composite may be a suitable alternative for bone tissue engineering scaffold.

Calcium Phosphate Ceramics for Bone Tissue Engineering

2007 ◽  
pp. 9-1-9-18
Author(s):  
Paul Spauwen ◽  
John Jansen ◽  
P QuintenRuhé ◽  
Joop Wolke
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Calcium Phosphate Ceramics

scholarly journals 3D Printed Polycaprolactone/Gelatin/Bacterial Cellulose/Hydroxyapatite Composite Scaffold for Bone Tissue Engineering

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 1962 ◽  
Author(s):  
Abdullah M. Cakmak ◽  
Semra Unal ◽  
Ali Sahin ◽  
Faik N. Oktar ◽  
Mustafa Sengor ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bacterial Cellulose ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Bone Tissue Regeneration ◽  
Engineering Applications ◽  
Bioactive Materials ◽  
Degradation Rates

Three-dimensional (3D) printing application is a promising method for bone tissue engineering. For enhanced bone tissue regeneration, it is essential to have printable composite materials with appealing properties such as construct porous, mechanical strength, thermal properties, controlled degradation rates, and the presence of bioactive materials. In this study, polycaprolactone (PCL), gelatin (GEL), bacterial cellulose (BC), and different hydroxyapatite (HA) concentrations were used to fabricate a novel PCL/GEL/BC/HA composite scaffold using 3D printing method for bone tissue engineering applications. Pore structure, mechanical, thermal, and chemical analyses were evaluated. 3D scaffolds with an ideal pore size (~300 µm) for use in bone tissue engineering were generated. The addition of both bacterial cellulose (BC) and hydroxyapatite (HA) into PCL/GEL scaffold increased cell proliferation and attachment. PCL/GEL/BC/HA composite scaffolds provide a potential for bone tissue engineering applications.

Effect of zirconia-mullite incorporated biphasic calcium phosphate/biopolymer composite scaffolds for bone tissue engineering

2020 ◽  
Vol 6 (5) ◽  
pp. 055004
Author(s):  
Tanawut Rittidach ◽  
Tanatsaparn Tithito ◽  
Panan Suntornsaratoon ◽  
Narattaphol Charoenphandhu ◽  
Jirawan Thongbunchoo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Biphasic Calcium Phosphate ◽  
Composite Scaffolds
Sign in / Sign up

Export Citation Format

Share Document