scholarly journals Identification of Self-Incompatibility Alleles by Specific PCR Analysis and S-RNase Sequencing in Apricot

2018 ◽  
Vol 19 (11) ◽  
pp. 3612 ◽  
Author(s):  
Sara Herrera ◽  
Javier Rodrigo ◽  
José Hormaza ◽  
Jorge Lora
Keyword(s):  
Fruit Production ◽  
Prunus Armeniaca ◽  
Pcr Analysis ◽  
Self Incompatibility ◽  
Breeding Programs ◽  
Specific Pcr ◽  
Incompatibility Alleles ◽  
S Allele ◽  
First Time ◽  
Efficient Mechanisms

Self-incompatibility (SI) is one of the most efficient mechanisms to promote out-crossing in plants. However, SI could be a problem for fruit production. An example is apricot (Prunus armeniaca), in which, as in other species of the Rosaceae, SI is determined by an S-RNase-based-Gametophytic Self-Incompatibility (GSI) system. Incompatibility relationships between cultivars can be established by an S-allele genotyping PCR strategy. Until recently, most of the traditional European apricot cultivars were self-compatible but several breeding programs have introduced an increasing number of new cultivars whose pollination requirements are unknown. To fill this gap, we have identified the S-allele of 44 apricot genotypes, of which 43 are reported here for the first time. The identification of Sc in 15 genotypes suggests that those cultivars are self-compatible. In five genotypes, self-(in)compatibility was established by the observation of pollen tube growth in self-pollinated flowers, since PCR analysis could not allowed distinguishing between the Sc and S8 alleles. Self-incompatible genotypes were assigned to their corresponding self-incompatibility groups. The knowledge of incompatibility relationships between apricot cultivars can be a highly valuable tool for the development of future breeding programs by selecting the appropriate parents and for efficient orchard design by planting self-compatible and inter-compatible cultivars.

scholarly journals Identification of Self-incompatibility Genotypes in Apple and Crabapple Cultivars by S-allele Specific PCR Analysis

2014 ◽  
Vol 46 (4) ◽  
pp. 364-371
Author(s):  
Kang Hee Cho ◽  
Jeong-Hee Kim2 ◽  
Jung Woo Lee ◽  
Soon-Il Kwon ◽  
Jong Taek Park ◽  
...  
Keyword(s):  
Pcr Analysis ◽  
Self Incompatibility ◽  
Specific Pcr ◽  
Allele Specific ◽  
S Allele ◽  
Allele Specific Pcr

New self-incompatibility alleles in apricot (Prunus armeniaca L.) revealed by stylar ribonuclease assay and S-PCR analysis

Euphytica ◽  
2005 ◽  
Vol 145 (1-2) ◽  
pp. 57-66 ◽  
Author(s):  
Júlia Halász ◽  
Attila Hegedüs ◽  
Rita Hermán ◽  
Éva Stefanovits-Bányai ◽  
Andrzej Pedryc
Keyword(s):  
Prunus Armeniaca ◽  
Pcr Analysis ◽  
Self Incompatibility ◽  
Prunus Armeniaca L ◽  
Incompatibility Alleles

IDENTIFICATION OF SELF-INCOMPATIBILITY ALLELES IN SOME ALMOND GENOTYPES BY PCR ANALYSIS

2011 ◽  
pp. 629-633
Author(s):  
Y. Sharafi ◽  
S.A. Mohammadi ◽  
J. Hajilou ◽  
J. Dejampour
Keyword(s):  
Pcr Analysis ◽  
Self Incompatibility ◽  
Incompatibility Alleles

PCR ANALYSIS OF SELF-INCOMPATIBILITY ALLELES IN APPLE APPLIED TO LEAVES, SEED EMBRYOS AND IN VITRO SHOOTS

1998 ◽  
pp. 403-410
Author(s):  
G.A. Janssens ◽  
A.M. Van Haute ◽  
J. Keulemans ◽  
W. Broothaerts ◽  
W.F. Broekaert
Keyword(s):  
Pcr Analysis ◽  
Self Incompatibility ◽  
In Vitro Shoots ◽  
Incompatibility Alleles

scholarly journals Self-incompatibility alleles in Esatern European and Asian almond (Prunus dulcis) genotypes: a preliminary study

2012 ◽  
Vol 18 (2) ◽  
Author(s):  
B. Szikriszt ◽  
S. Ercisli ◽  
A. Hegedűs ◽  
J. Halász
Keyword(s):  
Prunus Dulcis ◽  
Natural Occurrence ◽  
Eastern European ◽  
Self Incompatibility ◽  
S Locus ◽  
Centre Of Origin ◽  
Incompatibility Alleles ◽  
S Allele ◽  
The Village ◽  
New Alleles

Almond [Prunus dulcis (Mill.) D. A. Webb.] as one of the oldest domesticated plants is thought to have originated in central Asia. Gametophytic self-incompatibility of almond is controlled by the highly polymorphic S-locus. The S-locus encodes for an S-ribonuclease (S-RNase) protein in the pistils, which degrades RNA in self-pollen tubes and hence stops their growing. This study was carried out to detect S-RNase allelic variants in Hungarian and Eastern European almond cultivars and Turkish wild growing seedlings, and characterize their S-allele pool. Five new alleles were identifi ed, S31H, S36-S39 in Eastern European local cultivars. The village Bademli and Akdamar island are two distinct places of almond natural occurrence in Turkey. Trees growing wild around Bademli city showed greater genetic diversity than those originated on Akdamar island. Many of the previously described 45 S-RNase alleles have been also detected in these regions. Homology searches revealed that Turkish almonds carried some P. webbii alleles indicating hybridization between the two cultivars and massive introgression events. Our results supply long-awaited information on almond S-allele diversity from regions between the main cultivation centres and the centre of origin of this species; and are discussed from the aspect of methodological developments and evolution of the cultivated almond.

scholarly journals Nonself-recognition-based self-incompatibility can alternatively promote or prevent introgression

2020 ◽  
Author(s):  
Alexander Harkness ◽  
Yaniv Brandvain
Keyword(s):  
Pollen Limitation ◽  
List Type ◽  
Self Incompatibility ◽  
Core Eudicots ◽  
S Alleles ◽  
Self Fertilization ◽  
Nonself Recognition ◽  
Negative Frequency Dependent Selection ◽  
Incompatibility Alleles ◽  
S Allele

1SummaryTraditionally, we expect that self-incompatibility alleles (S-alleles), which prevent self-fertilization, should benefit from negative-frequency dependent selection and rise to high frequency when introduced to a new population through gene flow. However, the most taxonomically widespread form of self-incompatibility, the ribonuclease-based system ancestral to the core eudicots, functions through nonself-recognition, which drastically alters the process of S-allele diversification.We analyze a model of S-allele evolution in two populations connected by migration, focusing on comparisons among the fates of S-alleles originally unique to each population and those shared among populations.We find that both shared and unique S-alleles originating from the population with more unique S-alleles were usually fitter than S-alleles from the population with fewer. Resident S-alleles were often driven extinct and replaced by migrant S-alleles, though this outcome could be averted by pollen limitation or biased migration.Nonself-recognition-based self-incompatibility will usually either disfavor introgression of S-alleles or result in the whole-sale replacement of S-alleles from one population with those from another.

scholarly journals Cost-Effective and Time-Efficient Molecular Assisted Selection for Ppv Resistance in Apricot Based on ParPMC2 Allele-Specific PCR

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1292 ◽  
Author(s):  
Ángela Polo-Oltra ◽  
Carlos Romero ◽  
Inmaculada López ◽  
María Luisa Badenes ◽  
Elena Zuriaga
Keyword(s):  
Woody Species ◽  
Cost Effective ◽  
Prunus Armeniaca ◽  
Host Susceptibility ◽  
Limiting Factor ◽  
Plum Pox Virus ◽  
Breeding Programs ◽  
Specific Pcr ◽  
Allele Specific ◽  
Ppv Resistance

Plum pox virus (PPV) is the most important limiting factor for apricot (Prunus armeniaca L.) production worldwide, and development of resistant cultivars has been proven to be the best solution in the long-term. However, just like in other woody species, apricot breeding is highly time and space demanding, and this is particularly true for PPV resistance phenotyping. Therefore, marker-assisted selection (MAS) may be very helpful to speed up breeding programs. Tightly linked ParPMC1 and ParPMC2, meprin and TRAF-C homology (MATH)-domain-containing genes have been proposed as host susceptibility genes required for PPV infection. Contribution of additional genes to PPV resistance cannot be discarded, but all available studies undoubtedly show a strong correlation between ParPMC2-resistant alleles (ParPMC2res) and PPV resistance. The ParPMC2res allele was shown to carry a 5-bp deletion (ParPMC2-del) within the second exon that has been characterized as a molecular marker suitable for MAS (PMC2). Based on this finding, we propose here a method for PPV resistance selection in apricot by combining high-throughput DNA extraction of 384 samples in 2 working days and the allele-specific genotyping of PMC2 on agarose gel. Moreover, the PMC2 genotype has been determined by PCR or by using whole-genome sequences (WGS) in 175 apricot accessions. These results were complemented with phenotypic and/or genotypic data available in the literature to reach a total of 325 apricot accessions. As a whole, we conclude that this is a time-efficient, cost-effective and straightforward method for PPV resistance screening that can be highly useful for apricot breeding programs.

scholarly journals Selection and Validation of Reference Genes for Quantitative RT-PCR Analysis in Corylus heterophylla Fisch. × Corylus avellana L.

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 159
Author(s):  
Sihao Hou ◽  
Tiantian Zhao ◽  
Dan Yang ◽  
Qing Li ◽  
Lisong Liang ◽  
...  
Keyword(s):  
Reference Genes ◽  
Expression Profiles ◽  
Pcr Analysis ◽  
Self Incompatibility ◽  
Cross Pollination ◽  
Reverse Transcription Pcr ◽  
The Stability ◽  
Corylus Avellana L ◽  
Suitable Reference ◽  
First Time

(1) Background: the species of Corylus have sporophytic type of self-incompatibility. Several genes related to recognition reaction between pollen and stigma have been identified in hazelnuts. To better understand the self-incompatibility (SI) response, we screened the suitable reference genes by using quantitative real-time reverse transcription PCR (qRT-PCR) analysis in hazelnut for the first time. (2) Methods: the major cultivar “Dawei” was used as material. A total of 12 candidate genes were identified and their expression profiles were compared among different tissues and in response to various treatments (different times after self- and cross-pollination) by RT-qPCR. The expression stability of these 12 candidate reference genes was evaluated using geNorm, NormFinder, BestKeeper, Delta Ct, and RefFinder programs. (3) Results: the comprehensive ranking of RefFinder indicated that ChaActin, VvActin,ChaUBQ14, and ChaEF1-α were the most suitable reference genes. According to the stability analysis of 12 candidate reference genes for each sample group based on four software packages, ChaActin and ChaEF1-α were most stable in different times after self-pollination and 4 h after self- and cross-pollination, respectively. To further validate the suitability of the reference genes identified in this study, CavPrx, which the expression profiles in Corylus have been reported, was quantified by using ChaActin and ChaEF1-α as reference genes. (4) Conclusions: our study of reference genes selection in hazelnut shows that the two reference genes, ChaActin and ChaEF1-α, are suitable for the evaluation of gene expression, and can be used for the analysis of pollen-pistil interaction in Corylus. The results supply a reliable foundation for accurate gene quantifications in Corylus species, which will facilitate the studies related to the reproductive biology in Corylus.

scholarly journals Detecting Cross-incompatibility of Three North American Apricot Cultivars and Establishing the First Incompatibility Group in Apricot

1996 ◽  
Vol 121 (6) ◽  
pp. 1002-1005 ◽  
Author(s):  
J. Egea ◽  
L. Burgos
Keyword(s):  
North American ◽  
Fruit Set ◽  
Common Ancestor ◽  
Prunus Armeniaca ◽  
Self Incompatibility ◽  
S Locus ◽  
Incompatibility Group ◽  
Breeding Programs ◽  
Multiple Alleles ◽  
Compatible Pollinations

Laboratory and orchard tests have shown that the apricot (Prunus armeniaca L.) cultivars `Hargrand', `Goldrich', and `Lambertin-1' are cross-incompatible. All three cultivars are from North American breeding programs and have `Perfection' as a common ancestor. In orchard tests, compatible pollinations resulted in 19% to 74% fruit set, while incompatible pollinations resulted in <2% fruit set. Microscopic examination showed that, in incompatible pollinations, pollen tube growth was arrested in the style, most frequently in its third quarter, and that the ovary was never reached. It is proposed that self-incompatibility in apricot is of the gametophytic type, controlled by one S-locus with multiple alleles, and that these three cultivars are S1S2.

Sign in / Sign up

Export Citation Format

Share Document