scholarly journals Dietary Supplementation with Trihexanoin Enhances Intestinal Function of Weaned Piglets

2018 ◽  
Vol 19 (10) ◽  
pp. 3277
Author(s):  
Tao Wu ◽  
Kang Li ◽  
Dan Yi ◽  
Lei Wang ◽  
Di Zhao ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Fat Metabolism ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Mucosal Barrier ◽  
Mrna Levels ◽  
Intestinal Function ◽  
Weaned Piglets ◽  
And Function

Trihexanoin is a short-chain triglyceride (SCT). Many studies have reported that SCTs play important roles in the maintenance of intestinal epithelial structure and function. The present work was to investigate the effects of trihexanoin on growth performance, carbohydrate and fat metabolism, as well as intestinal morphology and function in weaned piglets. Twenty weaned piglets (21 ± 2 d) were randomly allocated to one of two treatment groups: The control group (basal diet supplemented with 0.5% soya oil); the TH group (basal diet supplemented with 0.5% trihexanoin). Dietary trihexanoin supplementation significantly reduced diarrhea rate; increased the concentrations of LDL, HDL and total protein in plasma; decreased cholesterol concentrations and glutamyl transpeptidase activity in plasma; improved intestinal morphologic structure; altered the mRNA levels and abundances of proteins related to glycogen and fat metabolism, mucosal barrier function, antioxidant capacity and water transport capacity; and altered the community of intestinal microflora. These results indicate that dietary trihexanoin supplementation could reduce diarrhea, regulate carbohydrate and fat metabolism, exert beneficial effects on the intestinal mucosal barrier, protect the intestinal mucosa from injuries, improve intestinal transport and absorption, and enhance antioxidant capacity. In conclusion, dietary supplementation with 0.5% trihexanoin improves the intestinal function and health of weaned piglets.

scholarly journals PSVIII-18 Dietary supplementation with trihexanoin enhances intestinal function of weaned piglets

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 302-303
Author(s):  
Tao Wu
Keyword(s):  
Fat Metabolism ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Mucosal Barrier ◽  
Mrna Levels ◽  
Intestinal Function ◽  
Weaned Piglets ◽  
Villus Height ◽  
And Function

Abstract Trihexanoin is a short-chain triglyceride (SCT). Many studies have reported that SCTs play important roles in the maintenance of intestinal epithelial structure and function. The present work was to investigate the effects of trihexanoin on growth performance, carbohydrate and fat metabolism, as well as intestinal morphology and function in weaned piglets. Twenty weaned piglets (21 ± 2 d) were randomly allocated to one of two treatment groups: the control group (basal diet supplemented with 0.5% soya oil); the TH group (basal diet supplemented with 0.5% trihexanoin). Dietary trihexanoin supplementation significantly reduced diarrhea rate (P < 0.05); increased the concentrations of LDL, HDL and total protein, decreased cholesterol concentrations (CHOL) and glutamyl transpeptidase (GGT) activity in plasma (P < 0.05); increased villus height, surface area, and the ratio of villus height to crypt depth (P < 0.05); altered the mRNA levels and abundances of proteins related to glycogen and fat metabolism (gene LIPE, LPL, PPARG, ACACA, FASN, SLC27A2, INSR, PCK1 and ASS1), mucosal barrier function (protein claudin-1, and occludin), antioxidant capacity (protein HSP70 and gene Nrf2, NOX2 and GSTO2) and water transport capacity (protein AQP3 and gene AQP8 and AQP10) (P < 0.05); altered the gene abundance of intestinal bacteria (Enterobacteriaceae, Enterococcus, Clostridium, Lactobacillus and Bifidobacterium) (P < 0.05). In conclusion, dietary supplementation of trihexanoin improved the intestinal function and health of weaned piglets by regulating nutrient metabolism, improving intestinal function of mucosal barrier, transport, absorption and antioxidant, and altering the community of microbiota.

scholarly journals PSVI-40 Effects of dietary glucan on intestinal morphology, immunity response, barrier function and antioxidant capacity in weaning pigs

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 212-213
Author(s):  
xia xiong ◽  
Lvliang Wu ◽  
Yirui Shao ◽  
Jian zou ◽  
Yulong Yin
Keyword(s):  
Antioxidant Capacity ◽  
Growth Performance ◽  
Mrna Expression ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Control Group ◽  
Gut Health ◽  
Weaned Piglets ◽  
Weaning Piglets

Abstract Glucan has been studied as a potential alternative to antibiotics for animals in recent years. The aim of this study was to evaluate the effect of dietary glucan on growth performance and gut health of weaning piglets, which is a water-soluble extracellular ꞵ-glucan produced by Agrobacterium sp. ZX09. A total of 108 weaned piglets (21 d of age; 6.05 ± 0.36 kg) were randomly assigned (6 pens/diet; 18 piglets/pen) to 3 dietary treatments consisting of a basal diet (control group) or the basal diet supplemented with 20 ppm olaquindox or 200 ppm glucan for 14 days, respectively. The results showed that piglets fed with glucan had greater (P < 0.05) body weight and average daily gain than piglets in control group. Piglets fed with glucan or antibiotic had greater villus height to crypt depth ratio on duodenum compared with control group (P < 0.05). The mRNA expression of Claudin-1 on duodenum or ileum was higher (P < 0.05) in glucan group than that on the other groups. The mRNA expression of TLR4, MYD88 and NFκB on jejunum were lower (P < 0.05) in glucan or antibiotic group than those in control group. Dietary supplementation with glucan tended to increase the IL-10 and SIgA concentration on ileum (0.05 < P < 0.1). Dietary supplementation with glucan tended to increase the total antioxidant capacity on jejunum (P = 0.093). In conclusion, 200 ppm glucan or 20 ppm olaquindox can improve the growth performance of weaning piglets. The glucan may can accelerate the growth of weaned piglets by improving gut health. This research will provide guidance for the olaquindox alternative on growing piglets.

scholarly journals The Evaluation of the Antioxidant and Intestinal Protective Effects of Baicalin-Copper in Deoxynivalenol-Challenged Piglets

2020 ◽  
Vol 2020 ◽  
pp. 1-13 ◽  
Author(s):  
Andong Zha ◽  
Daixiu Yuan ◽  
Zhijuan Cui ◽  
Ming Qi ◽  
Simeng Liao ◽  
...  
Keyword(s):  
Protein Expression ◽  
Antioxidant Capacity ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Mrna Levels ◽  
Protective Effects ◽  
Serum Concentrations ◽  
Weaned Piglets ◽  
Sod Activity ◽  
Growth Impairment

The present study was performed to evaluate the antioxidant and intestinal protective effects of baicalin-copper on deoxynivalenol-challenged piglets. Forty weaned piglets were randomly divided into four groups and assigned to different diets: (1) basal diet (Con), (2) 4 mg/kg deoxynivalenol of basal diet (DON), (3) 5 g/kg baicalin-copper of basal diet (BCU); and (4) 4 mg/kg deoxynivalenol+5 g/kg baicalin‐copper of basal diet (DBCU). The results showed that the ADFI and ADG of piglets in the DON group were markedly lower than those in the Con group, but the ADFI and ADG of the DBCU group were not significantly different from those of the Con group. In piglets fed a DON-contaminated diet, dietary supplementation with BCU significantly decreased the mRNA levels of P70S6K, 4E-BP1, and HSP70 in the liver, the protein expression of HO-1 in the jejunum, and the expression of p-Nrf2 and p-NF-κB in the ileum but increased Mn-SOD activity in serum. Dietary supplementation with BCU increased jejunal maltase, ZIP4 and MT mRNA levels, and serum concentrations of Arg, Val, Ile, Leu, Lys, and Tyr in DON-contaminated piglets. In summary, BCU can alleviate the growth impairment induced by DON and enhance antioxidant capacity and nutrition absorption in piglets fed DON-contaminated diets.

scholarly journals Dietary Supplementation With Bacillus subtilis Promotes Growth and Gut Health of Weaned Piglets

2021 ◽  
Vol 7 ◽  
Author(s):  
Zhilong Tian ◽  
Xiaodan Wang ◽  
Yehui Duan ◽  
Yue Zhao ◽  
Wenming Zhang ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Control Group ◽  
Gut Health ◽  
Weaned Piglets ◽  
Villus Height ◽  
Expression Of Genes ◽  
Group A

This study was conducted to investigate the effects of dietary supplementation with different types of Bacillus subtilis (B. subtilis) on the growth and gut health of weaned piglets. A total of 160 piglets were randomly assigned into four groups: control group (a basal diet), BS-A group (a basal diet supplemented with B. subtilis A at 1 × 106 CFU/g feed), BS-B group (a basal diet supplemented with B. subtilis B at 1 × 106 CFU/g feed), and BS-C group (a basal diet supplemented with B. subtilis C at 1 × 106 CFU/g feed). All groups had five replicates with eight piglets per replicate. On days 7, 21, and 42 of the trial, blood plasma and intestinal tissues and digesta samples were collected to determine plasma cytokine concentrations, intestinal morphology, gut microbiota community and metabolic activity, and the expression of genes related to gut physiology and metabolism. The results showed that dietary B. subtilis supplementation improved (P < 0.05) the body weight and average daily gain (in BS-B and BS-C groups) of weaned piglets and decreased (P < 0.05) the diarrhea rates (in BS-A, BS-B, and BS-C groups). In the intestinal morphology analysis, B. subtilis supplementation improved (P < 0.05) the size of villus height and villus height to crypt depth ratio in the ileum of weaned piglets. Firmicutes, Bacteroidetes, and Tenericutes were the most dominant microflora in piglets' colon whatever the trial group and time of analysis. Dietary BS-C supplementation increased (P < 0.05) the relative abundances of Anaerovibrio and Bulleidia and decreased (P < 0.05) the relative abundances of Clostridium and Coprococcus compared with the control group. In addition, dietary B. subtilis supplementation increased (P < 0.05) the indicators of intestinal health, including plasma levels of interleukin (IL)-2 and IL-10, as well as the colonic levels of short-chain fatty acids. Furthermore, dietary B. subtilis supplementation also up-regulated (P < 0.05) the expression of genes involved in metabolic pathways related to intestinal microbiota maturation. In conclusion, these findings suggest that a diet containing BS-B or BS-C can efficiently promote growth performance, decrease diarrhea incidence, and ameliorate several indicators of intestinal health through the modulation of gut microbiota composition and metabolic activity in weaned piglets.

scholarly journals Effects of Forsythia Suspense Extract as an Antibiotics Substitute on Growth Performance, Nutrient Digestibility, Serum Antioxidant Capacity, Fecal Escherichia coli Concentration and Intestinal Morphology of Weaned Piglets

Animals ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 729 ◽  
Author(s):  
Long ◽  
Liu ◽  
Liu ◽  
Mahfuz ◽  
Piao
Keyword(s):  
Escherichia Coli ◽  
Antioxidant Capacity ◽  
Phase 1 ◽  
Average Daily Gain ◽  
Basal Diet ◽  
Nutrient Digestibility ◽  
Intestinal Morphology ◽  
Phase 2 ◽  
Weaned Piglets ◽  
E Coli

The aim of this study is to determine the efficiency of Forsythia suspense extract (FSE) as an antibiotics substitute on performance, nutrient digestibility, serum antioxidant capacity, fecal Escherichia coli concentration and intestinal morphology of weaned piglets. A total of 108 Duroc × (Landrace × Yorkshire) weaned piglets (28 days (d) weaned, average body weight of 8.68 ± 1.36 kg) were randomly assigned into three dietary treatments, six pens per treatment, three barrows and three gilts per pen. The treatments contained a corn-soybean meal basal diet (CTR), an antibiotic diet (basal diet + 75 mg/kg chlortetracycline; CTC), and an FSE diet (basal diet + 200 mg/kg FSE; FSE). The experiment included phase 1 (d 1 to 14), phase 2 (d 15 to 28) and phase 3 (d 29 to 35). Compared with CTR, piglets fed FSE show improved (p < 0.05) average daily gain (ADG) and average daily feed intake in phase 2, as well as enhanced (p < 0.05) ADG from day 15 to 35 and day 1 to 28. Piglets supplemented with CTC and FSE showed a reduced (p < 0.05) diarrhea rate in phase 1, while piglets fed FSE showed enhanced (p < 0.05) apparent total tract digestibility (ATTD) of dry matter, organic matter, crude protein and gross energy, as well as lower (p < 0.05) nitrogen output in phase 2 compared with CTR and CTC. The content in the form of Colony-Forming Units (CFUs) of fecal E. coli on day 14 and 28 was lower (p < 0.05) in piglets fed FSE in comparison with CTR. The contents of total antioxidant capacity, superoxide dismutase and catalase in serum are enhanced (p < 0.05) compared with CTR and CTC, whereas the concentration of malondialdehyde in serum was decreased (p < 0.05) for piglets fed FSE on day 28 compared with CTC. The villus height to crypt depth ratio in ileum was numerically higher (p < 0.05) in piglets fed FSE in comparison with CTR. In conclusion, dietary FSE supplementation could substitute CTC in improving antioxidant capacity, nutrients digestibility and reducing fecal E. coli content, so as to reduce nitrogen output and diarrhea rate, and eventually improve performance in weaned piglets.

scholarly journals Trilactic Glyceride Benefits Intestinal Function of Weaned Piglets

2019 ◽  
Author(s):  
Tao Wu ◽  
Kang Li ◽  
Yang Lyu ◽  
Dan Yi ◽  
Di Zhao ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Molecular Mechanisms ◽  
Basal Diet ◽  
Blood Parameters ◽  
Intestinal Morphology ◽  
Control Group ◽  
Fatty Tissue ◽  
Weaned Piglets ◽  
And Function ◽  
The Impact

Both lactic acid and short chain fatty acid (SCFA) play important roles in maintenance of intestinal epithelial structure and function. Trilactic glyceride (TLG) obtains both excellences of lactic acid and SCFA. This study was to investigate the effects of trilactic glyceride on growth performance, blood parameters, liver function, intestinal morphology and intestine function of piglets. Twelve weaned piglets (21&plusmn;2 d) were randomly allocated to two treatment groups: 1) control group, piglets fed the basal diet; 2) TLG group, piglets fed the basal diet supplemented with 0.5 % TLG. On day 21 of the trial, D-xylose (0.1 g/kg&middot;BW) was orally administrated to all piglets and blood samples were collected 1 h thereafter. Then, all the piglets were sacrificed to examine intestinal mucosal morphology and collect fatty tissue, liver and intestinal mucosa for further analysis. The results showed that: compared with the control group, TLG group decreased blood ALB and GGT on day 10 and 20, TLG group decreased blood TP and increased blood TG on day 20 of the trail (p &lt; 0.05); TLG group decreased blood D-xylose and LDL, increased blood HDL (p &lt; 0.05). These data suggested that supplementing trilactic glyceride had beneficial impacts on promoting nutrients&rsquo; metabolism, maintaining intestinal integrity, and alleviating oxidative stress and diarrhoea. Further research of molecular mechanisms showed changing expression levels of related proteins and genes, suggesting that these could be involved in the regulation of the impact. The community composition of the gut microbiota was also found to be altered in several operational taxonomic units within the genus, Prevotella (order Bacteroidales), and the order, Clostridiales.

scholarly journals Effects of Dietary Supplementation of Lactobacillus delbrueckii on Gut Microbiome and Intestinal Morphology in Weaned Piglets

2021 ◽  
Vol 8 ◽  
Author(s):  
Xiao-Long Wang ◽  
Zhu-Ying Liu ◽  
Ying-Hui Li ◽  
Ling-Yuan Yang ◽  
Jie Yin ◽  
...  
Keyword(s):  
Growth Performance ◽  
Relative Abundance ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Isobutyric Acid ◽  
Intestinal Morphology ◽  
Lactobacillus Delbrueckii ◽  
Weaned Piglets ◽  
Experimental Time ◽  
And Performance

Lactobacillus delbrueckii is a Gram-positive bacterium mostly used in the dairy industry for yogurt and cheese. The present study was designed to evaluate the effects of Lactobacillus delbrueckii on serum biochemical parameters, intestinal morphology, and performance by supplementing at a dietary level of 0.1% in diets for weaned piglets. Eighty healthy weaned piglets (initial body weight: 7.56 ± 0.2 kg) were randomly divided into two feeding groups with four replicates in each group (n = 10 animals per replicate); piglets were fed with basal diet (CON) or basal diet containing 0.1% Lactobacillus delbrueckii (LAC). The results showed that dietary supplementation of Lactobacillus delbrueckii improved growth performance and increased serum HDL and insulin levels in piglets on the 28th day of the experimental time (p &lt; 0.05). The gut microbe analysis revealed that Lactobacillus delbrueckii significantly decreased the relative abundance of the phyla Bacteroidetes, but increased the relative abundance of the phyla Firmicutes. The Lactobacillus delbrueckii also significantly increased the relative abundance of Bifidobacterium and Lactobacillus at the genus level of the bacterial community in the ileum, but decreased the relative abundance of unclassified Clostridiales. Moreover, Lactobacillus delbrueckii improved mucosal morphology by obtaining higher intestinal villus height (p &lt; 0.05), significantly increasing the concentrations of butyrate, isobutyric acid, and isovaleric acid in colonic chyme of piglets, but decreasing the intestinal pH at the duodenum and ileum on the 28th day of the experimental time. In conclusion, dietary supplementation of Lactobacillus delbrueckii in the diet of weaned piglets can improve intestinal morphology and modulate the microbiota community to promote growth performance.

scholarly journals Effects of Fermented Tea Residue on Fattening Performance, Meat Quality, Digestive Performance, Serum Antioxidant Capacity, and Intestinal Morphology in Fatteners

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 185 ◽  
Author(s):  
Xiaoqing Ding ◽  
Huaiyu Li ◽  
Zhiwei Wen ◽  
Yong Hou ◽  
Genliang Wang ◽  
...  
Keyword(s):  
Body Weight ◽  
Antioxidant Capacity ◽  
Meat Quality ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Nutrient Digestibility ◽  
Intestinal Morphology ◽  
The Other ◽  
Crypt Depth ◽  
Fattening Performance

This study investigated the dietary supplementation of tea residue fermented by Bacillus subtilis, Aspergillus niger, and Saccharomyces cerevisiae, to explore its effects on growth performance, digestion performance, meat quality, serum antioxidant capacity, and intestinal morphology in pigs bred for rapid growth, also known as fatteners. One hundred and ninety-two healthy “Duroc × Landrace × Yorkshire” ternary hybrid pigs (body weight 70 ± 1.0 kg) were randomly divided into four groups according to the feeding test requirements, with four replicates in each group, and 12 fatteners per replicate. The control group (CG) was fed the basal diet. Treatments 1 (T1), 2 (T2), and 3 (T3), comprising ratios of 10%, 15%, and 20% of tea residue were added to the basal diet. The test period was 60 days. The results showed that supplementation of FTR in fatteners’ diets increased final body weight (FBW), average daily gain (ADG), and feed conversion ratio (FCR) in the T1 and T2 groups (p < 0.05). Compared with the other groups, the lightness (L*) and pH were significantly affected in the T2 group (p < 0.05). Compared with the CG, dietary supplementation of FTR significantly increased the nutrient digestibility of crude protein (CP), ether extract (EE), calcium (Ca), and phosphorus (P), improved the lipase and trypsin activities, and reduced drip loss and the shear force of fatteners (p < 0.05). Glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) were significantly increased in the T2 and T3 groups compared with the other groups (p < 0.05). Supplementation of FTR in the jejunum significantly increased the villi height of the T2 group and the ratio of villi height to crypt depth of the FTR groups. Compared with the other two groups, the T2 and T3 groups significantly reduced the ratio of the villous height to crypt depth in the duodenum (p < 0.05). In conclusion, the tea residue after fermentation was shown to have beneficial effects on the fattening performance, digestion performance, meat quality, serum antioxidant capacity, and intestinal morphology of fatteners.

scholarly journals N-Acetylcysteine improves intestinal function and attenuates intestinal autophagy in piglets challenged with β-conglycinin

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Huiyun Wang ◽  
Chengcheng Li ◽  
Meng Peng ◽  
Lei Wang ◽  
Di Zhao ◽  
...  
Keyword(s):  
Peptide Transporter ◽  
Mucosal Barrier ◽  
Control Group ◽  
Mrna Levels ◽  
Intestinal Function ◽  
Villus Height ◽  
Human Infants ◽  
Fatty Acid Binding ◽  
Positive Effects ◽  
Mucosal Barrier Function

Abstractβ-Conglycinin (β-CG), an anti-nutritional factor, is a major allergen in soybeans to induce intestinal dysfunction and diarrhea in neonatal animals, including piglets and human infants. This study with a piglet model determined the effects of N-acetylcysteine (NAC) on intestinal function and autophagy in response to β-CG challenge. Twenty-four 12-day-old piglets (3.44 ± 0.28 kg), which had been weaned at 7 days of age and adapted for 5 days after weaning, were randomly allocated to the control, β-CG, and β-CG + NAC groups. Piglets in the control group were fed a liquid diet containing 10% casein, whereas those in the β-CG and β-CG + NAC groups were fed the basal liquid diets containing 9.5% casein and 0.5% β-CG for 2 days. Thereafter, pigs in the β-CG + NAC group were orally administrated with 50 mg (kg BW)−1 NAC for 3 days, while pigs in the other two groups were orally administrated with the same volume of sterile saline. NAC numerically reduced diarrhea incidence (− 46.2%) and the concentrations of hydrogen peroxide and malondialdehyde, but increased claudin-1 and intestinal fatty-acid binding protein (iFABP) protein abundances and activities of catalase and glutathione peroxidase in the jejunum of β-CG-challenged piglets. Although β-CG challenge decreased the villus height, villus height/crypt depth ratio, and mRNA levels of claudin-1 and occludin, no significant differences were observed in these indices between the control and β-CG + NAC groups, suggesting the positive effects of NAC supplementation on intestinal mucosal barrier function. Moreover, NAC increased the concentrations of citrulline and D-xylose in the plasma, as well as the expression of genes for aquaporin (AQP) 3, AQP4, peptide transporter 1 (PepT1), sodium/glucose co-transporter-1 (SGLT-1), potassium inwardly-rectifying channel, subfamily J, member 13 (KCNJ13), and solute carrier family 1 member 1 (SLC1A1) in the jejunum, demonstrating that NAC augmented intestinal metabolic activity and absorptive function. Remarkably, NAC decreased Atg5 protein abundance and the LC3II/LC3I ratio (an indicator of autophagy) in the jejunum of β-CG-challenged piglets. Taken together, NAC supplementation improved intestinal function and attenuated intestinal autophagy in β-CG-challenged piglets.

scholarly journals Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0245739
Author(s):  
Inês Vieira da Silva ◽  
Bárbara P. Soares ◽  
Catarina Pimpão ◽  
Rui M. A. Pinto ◽  
Teresa Costa ◽  
...  
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Amino Acid ◽  
Biochemical Parameters ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Mrna Levels ◽  
Promising Target ◽  
Dietary Treatments ◽  
Glycerol Permeability

The regulation of glycerol permeability in the gastrointestinal tract is crucial to control fat deposition, lipolysis and gluconeogenesis. Knowing that the amino acid glutamine is a physiological regulator of gluconeogenesis, whereas cystine promotes adiposity, herein we investigated the effects of dietary supplementation with glutamine and cystine on the serum biochemical parameters of piglets fed on amino acid-enriched diets, as well as on the transcriptional profile of membrane water and glycerol channels aquaporins (AQPs) in the ileum portion of the small intestine and its impact on intestinal permeability. Twenty male piglets with an initial body weight of 8.8 ± 0.89 kg were allocated to four dietary treatments (n = 5) and received, during a four week-period, a basal diet without supplementation (control) or supplemented with 8 kg/ton of glutamine (Gln), cystine (Cys) or the combination of the two amino acids in equal proportions (Gln + Cys). Most biochemical parameters were found improved in piglets fed Gln and Cys diet. mRNA levels of AQP3 were found predominant over the others. Both amino acids, individually or combined, were responsible for a consistent downregulation of AQP1, AQP7 and AQP10, without impacting on water permeability. Conversely, Cys enriched diet upregulated AQP3 enhancing basolateral membranes glycerol permeability and downregulating glycerol kinase (GK) of intestinal cells. Altogether, our data reveal that amino acids dietary supplementation can modulate intestinal AQPs expression and unveil AQP3 as a promising target for adipogenesis regulation.

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