scholarly journals Molecular Cloning and Expression of MnGST-1 and MnGST-2 from Oriental River Prawn, Macrobrachium nipponense, in Response to Hypoxia and Reoxygenation

2018 ◽  
Vol 19 (10) ◽  
pp. 3102 ◽  
Author(s):  
Lei Xu ◽  
Ming Yang ◽  
Hongtuo Fu ◽  
Shengming Sun ◽  
Hui Qiao ◽  
...  
Keyword(s):  
Intracellular Transport ◽  
Expression Profiles ◽  
Acute Hypoxia ◽  
Cloning And Expression ◽  
Glutathione S Transferase ◽  
Specific Expression ◽  
Macrobrachium Nipponense ◽  
Hypoxia Stress ◽  
Oriental River Prawn ◽  
Specific Expression Pattern

The glutathione-S-transferase (GST) superfamily includes seven classes, and different classes have different functions. GST superfamily members function in various processes including detoxification of xenobiotics, protection against oxidative damage, and intracellular transport of hormones, endogenous metabolites, and exogenous chemicals. Herein, to elucidate the tissue-specific expression pattern of GSTs in response to hypoxia stress, which induces cell death, we investigated the expression of GSTs in response to hypoxia and reoxygenation in oriental river prawn, Macrobrachium nipponense. Full-length cDNAs of two δ class GSTs were cloned from the hepatopancreas, and named MnGST-1 and MnGST-2 based on the established GST nomenclature system. Expression profiles of both GSTs in various tissues were different under acute and chronic experimental hypoxia stress conditions, suggesting that both respond strongly to hypoxia-induced oxidative stress. However, the intensity of responses to hypoxia and reoxygenation were different in different tissues. During acute hypoxia stress, MnGST-1 responds earlier than MnGST-2 in the hepatopancreas and gill, but more slowly in muscle. By contrast, during chronic hypoxia stress, MnGST-2 plays a more important role in the hepatopancreas and gill than MnGST-1.

Effect of bisphenol A exposure on the expressions of five ovary development related genes in the oriental river prawn, Macrobrachium nipponense (Decapoda, Palaemonidae)

Crustaceana ◽  
2018 ◽  
Vol 91 (3) ◽  
pp. 321-334
Author(s):  
Xiu Li ◽  
Fengying Zhang ◽  
Keji Jiang ◽  
Weihong Zhao ◽  
Ming Zhao ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Fresh Water ◽  
Manufacturing Industry ◽  
Expression Profiles ◽  
Ovary Development ◽  
Control Group ◽  
Production Volume ◽  
Macrobrachium Nipponense ◽  
Severe Problem ◽  
Oriental River Prawn

The oriental river prawn,Macrobrachium nipponense, is one of the important commercial shrimp species. Recently a severe problem of precocious maturation has greatly hampered this shrimp aquaculture industry. Bisphenol A (BPA), a high-production-volume chemical substance used in the plastic manufacturing industry, has been thought of as an endocrine disruptor on the developmental processes of animals. In this study, we investigated the effect of BPA exposure on the expressions of five ovary development related genes such as Mago nashi, Tsunagi, Gustavus, Ubc9, and Von Willebrand factor D-Kazal inM. nipponense. Five concentration gradients (5.01, 7.76, 12.06, 18.62 and 28.84 mg/l) of BPA were set and the ovaries of exposed prawns were collected at different time points for expression analysis. Compared with the control group at day 19, BPA had a two-phase effect: a stimulating effect under low concentrations from 5.01 to 12.06 mg/l, whereas a negative effect was noted at high concentrations from 12.06 to 28.84 mg/l. The expression profiles under different BPA concentrations significantly changed along with the extension of exposure time. The medium concentration of BPA (12.06 mg/l) had a persistent influence on the expressions of the transcripts, while the effect was transient under the lowest concentration (5.01 mg/l). It is suggested that the concentration under 5.01 mg/l might be safe for the development ofM. nipponense, but exceeding 12.06 mg/l may be harmful. When exposed to clean fresh water without BPA, the gene expressions rebounded a little. This may indicate that the biological damage of BPA was partly reversible after the prawns had been placed in the fresh water without BPA. So the BPA pollutant concentration should be controlled at secure levels in order to ensure safety in aquaculture, in this respect. This study provides fundamental data for the relationship between BPA and precocious maturation of the prawn, and will most probably contribute to the understanding of the sexual maturation process in crustaceans.

scholarly journals Comprehensive Annotation and Functional Exploration of MicroRNAs in Lettuce

2021 ◽  
Vol 12 ◽  
Author(s):  
Yang Deng ◽  
Yajuan Qin ◽  
Pan Yang ◽  
Jianjun Du ◽  
Zheng Kuang ◽  
...  
Keyword(s):  
Regulatory Network ◽  
Regulatory Networks ◽  
Target Genes ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Computational Prediction ◽  
Regulatory Relationship ◽  
Specific Expression ◽  
Economic Significance ◽  
Specific Expression Pattern

MicroRNA (miRNA) is an important endogenous post-transcriptional regulator, while lettuce (Lactuca sativa) is a leafy vegetable of global economic significance. However, there are few studies on miRNAs in lettuce, and research on miRNA regulatory network in lettuce is absent. In this study, through deep sequencing of small RNAs in different tissues, together with a reference genome, 157 high-confidence miRNA loci in lettuce were comprehensively identified, and their expression patterns were determined. Using a combination of computational prediction and high-throughput experimental verification, a set of reliable lettuce miRNA targets were obtained. Furthermore, through RNA-Seq, the expression profiles of these targets and a comprehensive view of the negative regulatory relationship between miRNAs and their targets was acquired based on a correlation analysis. To further understand miRNA functions, a miRNA regulatory network was constructed, with miRNAs at the core and combining transcription factors and miRNA target genes. This regulatory network, mainly composed of feed forward loop motifs, greatly increases understanding of the potential functions of miRNAs, and many unknown potential regulatory links were discovered. Finally, considering its specific expression pattern, Lsa-MIR408 as a hub gene was employed to illustrate the function of the regulatory network, and genetic experiments revealed its ability to increase the fresh weight and achene size of lettuce. In short, this work lays a solid foundation for the study of miRNA functions and regulatory networks in lettuce.

Molecular cloning and expression analysis of two type II crustin genes in the oriental river prawn, Macrobrachium nipponense

2020 ◽  
Vol 98 ◽  
pp. 446-456 ◽  
Author(s):  
Xiaoling Dai ◽  
Xin Huang ◽  
Zhuoxing Zhang ◽  
Ruidong Zhang ◽  
Xueying Cao ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Expression Analysis ◽  
Cloning And Expression ◽  
Type Ii ◽  
Macrobrachium Nipponense ◽  
Oriental River Prawn
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