scholarly journals Time-Course of Metabolic and Proteomic Responses to Different Nitrate/Ammonium Availabilities in Roots and Leaves of Maize

2018 ◽  
Vol 19 (8) ◽  
pp. 2202 ◽  
Author(s):  
Bhakti Prinsi ◽  
Luca Espen
Keyword(s):  
Time Course ◽  
Biomass Accumulation ◽  
Organic N ◽  
N Availability ◽  
Cell Wall Metabolism ◽  
Pep Carboxylase ◽  
Total N ◽  
Metabolic Interactions ◽  
Ammonium Nutrition ◽  
Proteomic Responses

The availability of nitrate and ammonium significantly affects plant growth. Co-provision of both nutrients is generally the best nutritional condition, due to metabolic interactions not yet fully elucidated. In this study, maize grown in hydroponics was exposed to different nitrogen (N) availabilities, consisting of nitrate, ammonium and co-provision. Roots and leaves were analyzed after 6, 30, and 54 h by biochemical evaluations and proteomics. The ammonium-fed plants showed the lowest biomass accumulation and the lowest ratio of inorganic to organic N content, suggesting a metabolic need to assimilate ammonium that was not evident in plants grown in co-provision. The N sources differently affected the root proteome, inducing changes in abundance of proteins involved in N and carbon (C) metabolisms, cell water homeostasis, and cell wall metabolism. Notable among these changes was that some root enzymes, such as asparagine synthetase, phosphoenolpyruvate (PEP) carboxylase, and formate dehydrogenase showed a relevant upsurge only under the sole ammonium nutrition. However, the leaf proteome appeared mainly influenced by total N availability, showing changes in the abundance of several proteins involved in photosynthesis and in energy metabolism. Overall, the study provides novel information about the biochemical determinants involved in plant adaptation to different N mineral forms.

AVAILABILITY OF NITROGEN FROM THREE MANURES TO CORN IN THE FIELD

1986 ◽  
Vol 66 (4) ◽  
pp. 713-720 ◽  
Author(s):  
E. G. BEAUCHAMP
Keyword(s):  
Zea Mays ◽  
Zea Mays L ◽  
Cattle Manure ◽  
N Immobilization ◽  
Organic N ◽  
Yield Response ◽  
N Availability ◽  
Net N Mineralization ◽  
Yield Data ◽  
Total N

Three manures were compared with urea as sources of nitrogen for corn (Zea mays L.) on a different field site in each of 3 yr. The manures and their average [Formula: see text]–N:total N ratios were as follows: liquid poultry manure (LPM), 0.89; liquid dairy cattle manure (LCM), 0.53; and solid beef cattle manure (SBM), 0.09. The manures were applied at rates of 100, 200 and 300 kg total N ha−1. An additional LCM treatment of 600 kg total N ha−1 was also included. For comparison with the manures as N sources, urea was applied at rates of 50, 100 and 150 kg N ha−1. The yield response data were examined on the basis of a previously suggested model which predicted that all of the [Formula: see text]–N and part (e.g., 10–20%) of the organic N in manures are available for crop growth in the field. Regression analyses of paired yield data sets of urea and LCM or urea and LPM indicated that only 75–80% of the [Formula: see text]–N fraction applied in these manures was equivalent to urea-N. Thus, it was concluded that the model did not take into account net N immobilization and possibly N losses through denitrification following application. It was concluded also that N release from the organic N fraction of SBM differed substantially from that for the other manures. This conclusion was supported by greenhouse data which indicated that net N immobilization occurred for the first crop shortly after SBM was applied but this was followed by net N mineralization for a second crop as manure decomposition continued. Soil NO3− concentrations in mid-June generally increased with the urea, LPM and LCM sources of N at the higher rates of application in the field. Lower soil NO3− concentrations with SBM reflected the lower availability of N. Key words: Corn, manure N availability, Zea mays L.

scholarly journals Use of Digestate as Organic Amendment and Source of Nitrogen to Vegetable Crops

2021 ◽  
Vol 12 (1) ◽  
pp. 248
Author(s):  
Carmo Horta ◽  
João Paulo Carneiro
Keyword(s):  
Organic Amendment ◽  
Application Rate ◽  
Green Energy ◽  
Organic N ◽  
N Availability ◽  
Vegetable Crops ◽  
Mineral N ◽  
Total N ◽  
Ni Addition ◽  
Stable Organic Matter

Anaerobic digestion is a valuable process to use livestock effluents to produce green energy and a by-product called digestate with fertilising value. This work aimed at evaluating the fertilising value of the solid fraction (SF) of a digestate as an organic amendment and as a source of nitrogen to crops replacing mineral N. A field experiment was done with two consecutive vegetable crops. The treatments were: a control without fertilisation; Ni85 mineral fertilisation with 85 kg ha−1 of mineral N; fertiliser with digestate at an increasing nitrogen application rate (kg N ha−1): DG-N85 DG-N170, DG-N170+85, DG-N170+170; fertilisation with digestate together with Ni: DG-N85+Ni60, DG-N170+Ni60, DG-N170+Ni25. The results showed a soil organic amendment effect of the SF with a beneficial effect on SOM, soil pH and exchangeable bases. The SF was able to replace part of the mineral N fertilisation. The low mineralisation of the stable organic matter together with some immobilisation of mineral N from SF caused low N availability. The fertilisation planning should consider the SF ratio between the organic N (NO) and total N (TKN). Low NO:TKN ratios (≈0.65) needed lower Ni addition to maintaining the biomass production similar to the mineral fertilisation.

Short-term nitrogen dynamics in a soil amended with anaerobic digestate

2019 ◽  
Vol 99 (2) ◽  
pp. 173-181
Author(s):  
Mehdi Sharifi ◽  
Scott Baker ◽  
Leila Hojabri ◽  
Monireh Hajiaghaei-Kamrani
Keyword(s):  
Plant Biomass ◽  
Block Design ◽  
N Uptake ◽  
Organic N ◽  
N Recovery ◽  
N Availability ◽  
Silty Clay ◽  
Total N ◽  
Greenhouse Study ◽  
Mineralizable N

The co-product of anaerobic digestion, digestate, is nitrogen (N) rich; however, the forms and accessibility of this N by the crops have not been fully explored. This study aimed to determine the mineralization parameters of digestate N and to assess its availability for annual ryegrass (Lolium multiflorum Lam.). Four digestate rates of 0 (control), 38, 75, and 150 mg N kg−1 soil (equal to 0, 90, 180, and 360 kg total N ha−1) were applied to a silty clay loam soil in a completely randomized block design with four replications in a greenhouse study. A 100 d aerobic incubation experiment was also conducted with 0 and 150 mg digestate N kg−1 rates at 25 °C. Digestate feedstock included cattle manure (28%), hay (15%), and silage corn (Zea mays L.; 57%). Total plant biomass and N uptake increased linearly with digestate application rate with average apparent N recovery of 37%. Potentially mineralizable N (N0) and mineralizable N rate constant (k) were not significantly different in digestate and control treatments; however, a flush of digestate organic N (30 mg N kg−1) released right after mixing the digestate with soil. Evidences of N immobilization with digestate application were observed in greenhouse study. Majority of plant-available digestate N was in form of NH4+-N; therefore, NH4+-N can be used for estimation of available digestate N for crops. Results need to be validated for specific feedstock and soil properties under field conditions. Further research is needed to assess how long-term build-up of digestate organic N may impact the N availability for crops.

scholarly journals Effects of Bamboo (Phyllostachys praecox) Cultivation on Soil Nitrogen Fractions and Mineralization

Forests ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 1109
Author(s):  
Zhuangzhuang Qian ◽  
Xiao Sun ◽  
Jianshuang Gao ◽  
Shunyao Zhuang
Keyword(s):  
Land Use ◽  
Land Use Change ◽  
Soil Ph ◽  
Organic Material ◽  
Dominant Factor ◽  
Organic N ◽  
N Availability ◽  
Total N ◽  
Phyllostachys Praecox ◽  
N Fractions

The mineralization of soil organic nitrogen (N) is the key process in the cycling of N in terrestrial ecosystems. Land-use change to bamboo (Phyllostachys praecox) cultivation that later entails organic material mulching combined with chemical fertilizer application will inevitably influence soil N mineralization (Nmin) and availability dynamics. However, the soil Nmin rates associated with various N fractions of P. praecox in response to land-use change and mulching are not well understood. The present study aimed to understand the effects of land-use change to P. praecox bamboo cultivation and organic material mulching on soil Nmin and availability. Soil properties and organic N fractions were measured in a P. praecox field planted on former paddy fields, a mulched P. praecox field, and a rice (Oryza sativa L.) field. Soil Nmin was determined using a batch incubation method, with mathematical models used to predict soil Nmin kinetics and potential. The conversion from a paddy field to P. praecox plantation decreased the soil pH, soil total N, and soil organic matter (SOM) content significantly (p < 0.05); the mulching method induced further soil acidification. The mulching treatment significantly augmented the SOM content by 7.08% compared with the no-mulching treatment (p < 0.05), but it decreased soil hydrolyzable N and increased the nonhydrolyzable N (NHN) content. Both the Nmin rate and cumulative mineralized N were lowest in the mulched bamboo field. The kinetics of Nmin was best described by the ‘two-pool model’ and ‘special model’. The Pearson’s correlation analysis and the Mantel test suggested soil pH was the dominant factor controlling the soil cumulative mineralized N and mineralization potential in the bamboo fields. These findings could help us better understand the N cycling and N availability under mulching conditions for shifts in land use, and provide a scientific basis for the sustainable management of bamboo plantations.

scholarly journals Management Drives Differences in Nutrient Dynamics in Conventional and Organic Four-Year Crop Rotation Systems

Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 764
Author(s):  
Sharon L. Weyers ◽  
David W. Archer ◽  
Jane M.F. Johnson ◽  
Alan R. Wilts
Keyword(s):  
Nutrient Dynamics ◽  
Cropping Systems ◽  
Triticum Aestivum L ◽  
Animal Manure ◽  
Organic N ◽  
N Availability ◽  
Mineral N ◽  
Environmental Liabilities ◽  
Total N ◽  
Organic C

Application of exogenous N fertilizers provides agronomic benefits but carries environmental liabilities. Managing benefits and liabilities of N-based fertilizers in conventional (CNV) and organic (ORG) cropping systems might be improved with better knowledge of nutrient dynamics, the generation of intrinsic N, and maintenance of soil organic matter. This study evaluated mineral N dynamics, yields, residue inputs, and change in soil organic C (SOC) and total N (TN) in strip-tilled, four-year crop rotations [corn (Zea mays L.)-soybean (Glycine max [L.] Merr.)-wheat under-seeded with alfalfa (Triticum aestivum L./Medicago sativa L.)-alfalfa] over eight years of production under CNV management using mineral-N (NO3NH4) and chemical pesticides or ORG management using organic-N (animal manure) and no chemical treatments. In ORG, N availability increased over time, but did not benefit ORG yields due to poor control of insects and weeds. Corn, soybean, and wheat grain yields were 1.9 to 2.7 times greater in CNV. In general, SOC was lost in CNV but did not change in ORG. Cumulative yield N removals exceeded cumulative fertilizer-N inputs by an average of 78% in CNV and 64% in ORG. These results indicated ORG management supported N availability by generating intrinsic N.

scholarly journals Supplementary Potassium Sustains Fruit Yield in Bell Pepper under High Ammonium Nutrition

HortScience ◽  
2013 ◽  
Vol 48 (12) ◽  
pp. 1530-1536 ◽  
Author(s):  
Elvia Hernández-Gómez ◽  
Luis A. Valdez-Aguilar ◽  
Ana M. Castillo-González ◽  
María T. Colinas-León ◽  
Donita L. Cartmill ◽  
...  
Keyword(s):  
Nutrient Solution ◽  
Biomass Accumulation ◽  
Bell Pepper ◽  
Total N ◽  
Plant Parts ◽  
Growing Medium ◽  
Ammonium Nutrition ◽  
High Concentrations ◽  
K Concentration ◽  
Pepper Plants

The uptake of nitrogen (N) in nitrate or ammonium (NH4+) form affects physiological and metabolic processes and toxicity may develop in plants receiving high concentrations of NH4+. The objective of the present study was to delineate the response of bell pepper plants to varying proportions of NH4+ combined with increasing concentrations of potassium (K) in the nutrient solution. Bell pepper plants were tolerant to moderate proportions of NH4+ (25% or less or 50% or less); however, higher proportions resulted in growth reduction. The application of higher K concentrations in the nutrient solution did not ameliorate the growth on vegetative plant parts; however, when K was increased to 9 mm, the yield was sustained even when 50% of total N was in the NH4+ form. Decreased shoot:root ratio and harvest index indicated that biomass accumulation was affected more in the shoot than in the root and in the fruit than in the shoot, respectively. There was a lower concentration of NH4+ in the roots compared with leaves, suggesting that the higher K concentration that resulted from the increased K in the nutrient solution was associated with NH4+ translocation through the xylem. A decrease in calcium and magnesium detected in leaves suggests an antagonistic relationship with NH4+ and K in the nutrient solution, which was correlated with the acidification of the growing medium. Higher yields when K was at 9 mm may be the result of the high photosynthetic rate and stomatal conductance (gS) detected in plants fertigated with 25% of total N as NH4+ and the higher leaf water potential when the proportion of NH4+ was 50%. The biochemical composition of fruits was affected because both high NH4+ and increased K resulted in higher ethylene production, lipid peroxidation, superoxide dismutase activity, and carotenoids.

scholarly journals Using SPAD-meter in Nitrogen Fertilization of Rosa chinensis Jacq. var. mutabilis

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 969D-970 ◽  
Author(s):  
Giampaolo Zanin ◽  
Paolo Sambo
Keyword(s):  
Dry Weight ◽  
Nutrient Status ◽  
Organic N ◽  
N Availability ◽  
Plant Weight ◽  
Total N ◽  
Plant Organs ◽  
Rosa Chinensis ◽  
Status Assessment ◽  
Spad Meter

Rosa chinensis Jacq. var. mutabilis plants were grown in a greenhouse to determine whether a hand-held chlorophyll meter (SPAD-meter) is suitable for the plant N status assessment. Therefore, plants were fertilized with increasing levels of N, applied through urea form as top dressing. The doses were: 0, 0.15, 0.3, 0.45, 0.6, and 0.75 g of N per liter of substrate. Periodically during the growing season, plant height and width, fresh and dry weight of different plant organs at 10, 20, and 30 weeks after planting, and their total N, plant P, and K were measured. Furthermore, six times along the growing cycle, the amount of chlorophyll in leaves was estimated using a SPAD-meter and analytically measured by chlorophyll extraction with ethanol and reading through a spectrophotometer. In the same leaves, N concentration was also determined. Treatments with 0.45–0.6 g of N per liter of substrate gave the tallest and widest plant. Plant weight and flower production were also the highest with these doses. The concentration of organic N in plant organs increased along with the N availability in the substrate, which suggests that a “luxury consumption” took place. The SPAD values showed high correlation among chlorophyll and N concentrations. Values that ranged between 35–40 seemed to mean good nutrient status. A high correlation was also found among SPAD values and some of the productive characteristics, which indicates that a SPAD-meter is a suitable tool in the dynamic fertilization of rose.

scholarly journals Nitrogen fertilizer value of animal slurries with different proportions of liquid and solid fractions: A 3-year study under field conditions

2021 ◽  
pp. 1-11
Author(s):  
Betina Nørgaard Pedersen ◽  
Bent T. Christensen ◽  
Luca Bechini ◽  
Daniele Cavalli ◽  
Jørgen Eriksen ◽  
...  
Keyword(s):  
Solid Fraction ◽  
Liquid Fraction ◽  
N Fertilizer ◽  
Organic N ◽  
First Year ◽  
Net N Mineralization ◽  
Pig Slurry ◽  
Total N ◽  
Plant Availability ◽  
Loamy Sand Soil

Abstract The plant availability of manure nitrogen (N) is influenced by manure composition in the year of application whereas some studies indicate that the legacy effect in following years is independent of the composition. The plant availability of N in pig and cattle slurries with variable contents of particulate matter was determined in a 3-year field study. We separated cattle and a pig slurry into liquid and solid fractions by centrifugation. Slurry mixtures with varying proportions of solid and liquid fraction were applied to a loamy sand soil at similar NH4+-N rates in the first year. Yields and N offtake of spring barley and undersown perennial ryegrass were compared to plots receiving mineral N fertilizer. The first year N fertilizer replacement value (NFRV) of total N in slurry mixtures decreased with increasing proportion of solid fraction. The second and third season NFRV averaged 6.5% and 3.8% of total N, respectively, for cattle slurries, and 18% and 7.5% for pig slurries and was not related to the proportion of solid fraction. The estimated net N mineralization of residual organic N increased nearly linearly with growing degree days (GDD) with a rate of 0.0058%/GDD for cattle and 0.0116%/GDD for pig slurries at 2000–5000 GDD after application. In conclusion NFRV of slurry decreased with increasing proportion of solid fraction in the first year. In the second year, NFRV of pig slurry N was significantly higher than that of cattle slurry N and unaffected by proportion between solid and liquid fraction.

scholarly journals Effects of two wood-based biochars on the fate of added fertilizer nitrogen—a 15N tracing study

2021 ◽  
Author(s):  
Subin Kalu ◽  
Gboyega Nathaniel Oyekoya ◽  
Per Ambus ◽  
Priit Tammeorg ◽  
Asko Simojoki ◽  
...  
Keyword(s):  
Plant Uptake ◽  
Plant Biomass ◽  
Application Rate ◽  
Control Treatment ◽  
Organic N ◽  
N Leaching ◽  
Soil N ◽  
Mineral N ◽  
Total N ◽  
Application Rates

AbstractA 15N tracing pot experiment was conducted using two types of wood-based biochars: a regular biochar and a Kon-Tiki-produced nutrient-enriched biochar, at two application rates (1% and 5% (w/w)), in addition to a fertilizer only and a control treatment. Ryegrass was sown in pots, all of which except controls received 15N-labelled fertilizer as either 15NH4NO3 or NH415NO3. We quantified the effect of biochar application on soil N2O emissions, as well as the fate of fertilizer-derived ammonium (NH4+) and nitrate (NO3−) in terms of their leaching from the soil, uptake into plant biomass, and recovery in the soil. We found that application of biochars reduced soil mineral N leaching and N2O emissions. Similarly, the higher biochar application rate of 5% significantly increased aboveground ryegrass biomass yield. However, no differences in N2O emissions and ryegrass biomass yields were observed between regular and nutrient-enriched biochar treatments, although mineral N leaching tended to be lower in the nutrient-enriched biochar treatment than in the regular biochar treatment. The 15N analysis revealed that biochar application increased the plant uptake of added nitrate, but reduced the plant uptake of added ammonium compared to the fertilizer only treatment. Thus, the uptake of total N derived from added NH4NO3 fertilizer was not affected by the biochar addition, and cannot explain the increase in plant biomass in biochar treatments. Instead, the increased plant biomass at the higher biochar application rate was attributed to the enhanced uptake of N derived from soil. This suggests that the interactions between biochar and native soil organic N may be important determinants of the availability of soil N to plant growth.

scholarly journals Genotypic Difference in the Responses to Nitrogen Fertilizer Form in Tibetan Wild and Cultivated Barley

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 595
Author(s):  
Shama Naz ◽  
Qiufang Shen ◽  
Jonas Lwalaba Wa Lwalaba ◽  
Guoping Zhang
Keyword(s):  
Wild Barley ◽  
Growth Parameters ◽  
Inorganic Nitrogen ◽  
N Uptake ◽  
Total Soluble Protein ◽  
Organic N ◽  
Genotypic Difference ◽  
N Availability ◽  
Tibetan Wild Barley ◽  
Cultivated Barley

Nitrogen (N) availability and form have a dramatic effect on N uptake and assimilation in plants, affecting growth and development. In the previous studies, we found great differences in low-N tolerance between Tibetan wild barley accessions and cultivated barley varieties. We hypothesized that there are different responses to N forms between the two kinds of barleys. Accordingly, this study was carried out to determine the response of four barley genotypes (two wild, XZ16 and XZ179; and two cultivated, ZD9 andHua30) under 4Nforms (NO3−, NH4+, urea and glycine). The results showed significant reduction in growth parameters such as root/shoot length and biomass, as well as photosynthesis parameters and total soluble protein content under glycine treatment relative to other N treatments, for both wild and cultivated barley, however, XZ179 was least affected. Similarly, ammonium adversely affected growth parameters in both wild and cultivated barleys, with XZ179 being severely affected. On the other hand, both wild and cultivated genotypes showed higher biomass, net photosynthetic rate, chlorophyll and protein in NO3− treatment relative to other three N treatments. It may be concluded that barley undisputedly grows well under inorganic nitrogen (NO3−), however in response to the organic N wild barley prefer glycine more than cultivated barely.

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