scholarly journals Organ and Tissue-Specific Localisation of Selected Cell Wall Epitopes in the Zygotic Embryo of Brachypodium distachyon

2018 ◽  
Vol 19 (3) ◽  
pp. 725 ◽  
Author(s):  
Alexander Betekhtin ◽  
Anna Milewska-Hendel ◽  
Joanna Lusinska ◽  
Lukasz Chajec ◽  
Ewa Kurczynska ◽  
...  
Keyword(s):  
Cell Wall ◽  
Zygotic Embryo ◽  
Brachypodium Distachyon ◽  
Tissue Specific ◽  
Cell Wall Epitopes

scholarly journals Cell wall proteomic datasets of stems and leaves of Brachypodium distachyon

Data in Brief ◽  
2021 ◽  
Vol 35 ◽  
pp. 106818
Author(s):  
Thibaut Douché ◽  
Benoît Valot ◽  
Thierry Balliau ◽  
Hélène San Clemente ◽  
Michel Zivy ◽  
...  
Keyword(s):  
Cell Wall ◽  
Brachypodium Distachyon ◽  
Stems And Leaves

scholarly journals Response of cell-wall composition and RNA-seq transcriptome to methyl-jasmonate in Brachypodium distachyon callus

Planta ◽  
2018 ◽  
Vol 248 (5) ◽  
pp. 1213-1229 ◽  
Author(s):  
Lucy S. Hyde ◽  
Till K. Pellny ◽  
Jackie Freeman ◽  
Louise V. Michaelson ◽  
Rachael Simister ◽  
...  
Keyword(s):  
Cell Wall ◽  
Methyl Jasmonate ◽  
Brachypodium Distachyon ◽  
Cell Wall Composition ◽  
Rna Seq

scholarly journals The Role of Brachypodium distachyon Wall-Associated Kinases (WAKs) in Cell Expansion and Stress Responses

Cells ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 2478
Author(s):  
Xingwen Wu ◽  
Antony Bacic ◽  
Kim L. Johnson ◽  
John Humphries
Keyword(s):  
Cell Wall ◽  
Stress Response ◽  
Plant Cell ◽  
Stress Responses ◽  
Cell Expansion ◽  
Plant Cell Wall ◽  
Brachypodium Distachyon ◽  
Critical Role ◽  
Cell Integrity

The plant cell wall plays a critical role in signaling responses to environmental and developmental cues, acting as both the sensing interface and regulator of plant cell integrity. Wall-associated kinases (WAKs) are plant receptor-like kinases located at the wall—plasma membrane—cytoplasmic interface and implicated in cell wall integrity sensing. WAKs in Arabidopsis thaliana have been shown to bind pectins in different forms under various conditions, such as oligogalacturonides (OG)s in stress response, and native pectin during cell expansion. The mechanism(s) WAKs use for sensing in grasses, which contain relatively low amounts of pectin, remains unclear. WAK genes from the model monocot plant, Brachypodium distachyon were identified. Expression profiling during early seedling development and in response to sodium salicylate and salt treatment was undertaken to identify WAKs involved in cell expansion and response to external stimuli. The BdWAK2 gene displayed increased expression during cell expansion and stress response, in addition to playing a potential role in the hypersensitive response. In vitro binding assays with various forms of commercial polysaccharides (pectins, xylans, and mixed-linkage glucans) and wall-extracted fractions (pectic/hemicellulosic/cellulosic) from both Arabidopsis and Brachypodium leaf tissues provided new insights into the binding properties of BdWAK2 and other candidate BdWAKs in grasses. The BdWAKs displayed a specificity for the acidic pectins with similar binding characteristics to the AtWAKs.

scholarly journals Transcriptional and Metabolomic Analyses Indicate that Cell Wall Properties are Associated with Drought Tolerance in Brachypodium distachyon

2019 ◽  
Vol 20 (7) ◽  
pp. 1758 ◽  
Author(s):  
Ingo Lenk ◽  
Lorraine Fisher ◽  
Martin Vickers ◽  
Aderemi Akinyemi ◽  
Thomas Didion ◽  
...  
Keyword(s):  
Cell Wall ◽  
Drought Tolerance ◽  
Plant Cell Wall ◽  
Brachypodium Distachyon ◽  
Glycoside Hydrolases ◽  
Drought Tolerant ◽  
Pectin Acetylesterase ◽  
Induced Changes ◽  
Cell Wall Properties ◽  
Go Terms

Brachypodium distachyon is an established model for drought tolerance. We previously identified accessions exhibiting high tolerance, susceptibility and intermediate tolerance to drought; respectively, ABR8, KOZ1 and ABR4. Transcriptomics and metabolomic approaches were used to define tolerance mechanisms. Transcriptional analyses suggested relatively few drought responsive genes in ABR8 compared to KOZ1. Linking these to gene ontology (GO) terms indicated enrichment for “regulated stress response”, “plant cell wall” and “oxidative stress” associated genes. Further, tolerance correlated with pre-existing differences in cell wall-associated gene expression including glycoside hydrolases, pectin methylesterases, expansins and a pectin acetylesterase. Metabolomic assessments of the same samples also indicated few significant changes in ABR8 with drought. Instead, pre-existing differences in the cell wall-associated metabolites correlated with drought tolerance. Although other features, e.g., jasmonate signaling were suggested in our study, cell wall-focused events appeared to be predominant. Our data suggests two different modes through which the cell wall could confer drought tolerance: (i) An active response mode linked to stress induced changes in cell wall features, and (ii) an intrinsic mode where innate differences in cell wall composition and architecture are important. Both modes seem to contribute to ABR8 drought tolerance. Identification of the exact mechanisms through which the cell wall confers drought tolerance will be important in order to inform development of drought tolerant crops.

scholarly journals Tissue-Specific Expression of a Gene Encoding a Cell Wall-Localized Lipid Transfer Protein from Arabidopsis

1994 ◽  
Vol 105 (1) ◽  
pp. 35-45 ◽  
Author(s):  
S. Thoma ◽  
U. Hecht ◽  
A. Kippers ◽  
J. Botella ◽  
S. De Vries ◽  
...  
Keyword(s):  
Cell Wall ◽  
Lipid Transfer Protein ◽  
Lipid Transfer ◽  
Gene Encoding ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Transfer Protein ◽  
A Cell

scholarly journals Tissue-Specific and Developmentally Regulated Expression of a Cluster of Tandemly Arrayed Cell Wall-Associated Kinase-Like Kinase Genes in Arabidopsis

2003 ◽  
Vol 133 (4) ◽  
pp. 1732-1746 ◽  
Author(s):  
Joseph A. Verica ◽  
Lee Chae ◽  
Hongyun Tong ◽  
Peter Ingmire ◽  
Zheng-Hui He
Keyword(s):  
Cell Wall ◽  
Developmentally Regulated ◽  
Tissue Specific ◽  
Regulated Expression

scholarly journals Differential Gene Expression of Brachypodium distachyon Roots Colonized by Gluconacetobacter diazotrophicus and the Role of BdCESA8 in the Colonization

2021 ◽  
Author(s):  
Xuan Yang ◽  
Kathleen A. Hill ◽  
Ryan S. Austin ◽  
Lining Tian
Keyword(s):  
Gene Expression ◽  
Cell Wall ◽  
Rna Sequencing ◽  
Crop Production ◽  
Brachypodium Distachyon ◽  
Cellulose Synthesis ◽  
Gibberellin Biosynthesis ◽  
Cellulose Content ◽  
Gluconacetobacter Diazotrophicus ◽  
Nitrogen Fixing

Alternatives to synthetic nitrogen fertilizer are needed to reduce the costs of crop production and offset environmental damage. Nitrogen-fixing bacterium Gluconacetobacter diazotrophicus has been proposed as a possible biofertilizer for monocot crop production. However, the colonization of G. diazotrophicus in most monocot crops is limited and deep understanding of the response of host plants to G. diazotrophicus colonization is still lacking. In this study, the molecular response of the monocot plant model Brachypodium distachyon was studied during G. diazotrophicus root colonization. The gene expression profiles of B. distachyon root tissues colonized by G. diazotrophicus were generated via next-generation RNA sequencing, and investigated through gene ontology and metabolic pathway analysis. The RNA sequencing results indicated that Brachypodium is actively involved in G. diazotrophicus colonization via cell wall synthesis. Jasmonic acid, ethylene, gibberellin biosynthesis. nitrogen assimilation, and primary and secondary metabolite pathways are also modulated to accommodate and control the extent of G. diazotrophicus colonization. Cellulose synthesis is significantly downregulated during colonization. The loss of function mutant for Brachypodium cellulose synthase 8 (BdCESA8) showed decreased cellulose content in xylem and increased resistance to G. diazotrophicus colonization. This result suggested that the cellulose synthesis of the secondary cell wall is involved in G. diazotrophicus colonization. The results of this study provide insights for future research in regard to gene manipulation for efficient colonization of nitrogen-fixing bacteria in Brachypodium and monocot crops. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

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