scholarly journals Comparison of Tumor- and Bone Marrow-Derived Mesenchymal Stromal/Stem Cells from Patients with High-Grade Osteosarcoma

2018 ◽  
Vol 19 (3) ◽  
pp. 707 ◽  
Author(s):  
Louis-Romée Le Nail ◽  
Meadhbh Brennan ◽  
Philippe Rosset ◽  
Frédéric Deschaseaux ◽  
Philippe Piloquet ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
High Grade ◽  
High Grade Osteosarcoma ◽  
Stromal Stem Cells

Recent approaches for isolating and culturing mouse bone marrow-derived mesenchymal stromal stem cells

2020 ◽  
Vol 15 ◽  
Author(s):  
Basem M. Abdallah ◽  
Hany M. Khattab
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Molecular Mechanisms ◽  
Replicative Senescence ◽  
Cellular Heterogeneity ◽  
High Yield ◽  
Mouse Strains ◽  
Mouse Bone Marrow ◽  
Differentiation Potential ◽  
Stromal Stem Cells

: The isolation and culture of murine bone marrow-derived mesenchymal stromal stem cells (mBMSCs) have attracted great interest in terms of the pre-clinical applications of stem cells in tissue engineering and regenerative medicine. In addition, culturing mBMSCs is important for studying the molecular mechanisms of bone remodelling using relevant transgenic mice. Several factors have created challenges in the isolation and high-yield expansion of homogenous mBMSCs; these factors include low frequencies of bone marrow-derived mesenchymal stromal stem cells (BMSCs) in bone marrow, variation among inbred mouse strains, contamination with haematopoietic progenitor cells (HPCs), the replicative senescence phenotype and cellular heterogeneity. In this review, we provide an overview of nearly all protocols used for isolating and culturing mBMSCs with the aim of clarifying the most important guidelines for culturing highly purified mBMSC populations retaining in vitro and in vivo differentiation potential.

scholarly journals Ex vivo gene therapy in autologous bone marrow stromal stem cells for tissue-engineered maxillofacial bone regeneration

Gene Therapy ◽  
2003 ◽  
Vol 10 (24) ◽  
pp. 2013-2019 ◽  
Author(s):  
S C-N Chang ◽  
H L Chuang ◽  
Y R Chen ◽  
J K Chen ◽  
H-Y Chung ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Bone Marrow ◽  
Bone Regeneration ◽  
Ex Vivo ◽  
Autologous Bone Marrow ◽  
Ex Vivo Gene Therapy ◽  
Autologous Bone ◽  
Stromal Stem Cells

scholarly journals In utero xenotransplantation of mice bone marrow-derived stromal/stem cells into fetal rat liver: A preliminary study

2020 ◽  
Author(s):  
Maryam Kasraeian ◽  
Elahe Ghasemi ◽  
Mehdi Dianatpour ◽  
Nader Tanideh ◽  
Iman Razeghian Jahromi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Liver Tissue ◽  
Fluorescent Protein ◽  
In Utero ◽  
Mouse Bone Marrow ◽  
Rat Fetus ◽  
Adult Mice ◽  
Infant Rats ◽  
Stromal Stem Cells

Background: Animals can play an important role in preparing tissues for human through the development of xenotransplantation protocols. The most common problem with liver transplantation like any other organ transplantation is organ supply shortage. Objective: To evaluate the in utero xenotransplantation of mouse bone marrow-derived stromal/stem cells (BMSCs) to the liver of rat fetus to produce mouse liver tissue. Materials and Methods: BMSCs were isolated and confirmed from enhanced green fluorescent protein (eGFP)-genetic labeled mice. Using a microinjection protocol, mice BMSCs were injected into the liver of rat fetuses in utero on day 14 of pregnancy. After birth, livers were collected and the presence of mice eGFP-positive cells in rat livers was evaluated through polymerase chain reaction. Results: The eGFP mRNA was detected in the liver of injected infant rats. BMSCs of adult mice were capable to remain functional probably as hepatocyte-like cells in liver of infant rats after in utero xenotransplantation. Conclusion: BMSCs have the potential for intrauterine xenotransplantation for the treatment of liver dysfunction before birth. This method can also be used for xenoproduction of liver tissue for transplantation. Key words: Xenotransplantation, Liver, Bone marrow, Stromal/stem cell, Murine.

scholarly journals Obesity-Associated Hypermetabolism and Accelerated Senescence of Bone Marrow Stromal Stem Cells Suggest a Potential Mechanism for Bone Fragility

Cell Reports ◽  
2019 ◽  
Vol 27 (7) ◽  
pp. 2050-2062.e6 ◽  
Author(s):  
Michaela Tencerova ◽  
Morten Frost ◽  
Florence Figeac ◽  
Tina Kamilla Nielsen ◽  
Dalia Ali ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Fragility ◽  
Potential Mechanism ◽  
Accelerated Senescence ◽  
Stromal Stem Cells

Glucocorticoids impair bone formation of bone marrow stromal stem cells by reciprocally regulating microRNA-34a-5p

2015 ◽  
Vol 27 (4) ◽  
pp. 1493-1505 ◽  
Author(s):  
H. Kang ◽  
H. Chen ◽  
P. Huang ◽  
J. Qi ◽  
N. Qian ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Formation ◽  
Stromal Stem Cells

Resveratrol inhibits adipocyte differentiation and cellular senescence of human bone marrow stromal stem cells

Bone ◽  
2020 ◽  
Vol 133 ◽  
pp. 115252 ◽  
Author(s):  
Dalia Ali ◽  
Li Chen ◽  
Justyna M. Kowal ◽  
Meshail Okla ◽  
Muthurangan Manikandan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cellular Senescence ◽  
Adipocyte Differentiation ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Stromal Stem Cells

scholarly journals CRISPR/Cas9-mediated reversibly immortalized mouse bone marrow stromal stem cells (BMSCs) retain multipotent features of mesenchymal stem cells (MSCs)

Oncotarget ◽  
2017 ◽  
Vol 8 (67) ◽  
pp. 111847-111865 ◽  
Author(s):  
Xue Hu ◽  
Li Li ◽  
Xinyi Yu ◽  
Ruyi Zhang ◽  
Shujuan Yan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Mouse Bone Marrow ◽  
Stromal Stem Cells
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