scholarly journals Bioreducible Polymer Micelles Based on Acid-Degradable Poly(ethylene glycol)-poly(amino ketal) Enhance the Stromal Cell-Derived Factor-1α Gene Transfection Efficacy and Therapeutic Angiogenesis of Human Adipose-Derived Stem Cells

2018 ◽  
Vol 19 (2) ◽  
pp. 529 ◽  
Author(s):  
Tae-Jin Lee ◽  
Min Shim ◽  
Taekyung Yu ◽  
Kyunghee Choi ◽  
Dong-Ik Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ethylene Glycol ◽  
Stromal Cell ◽  
Gene Transfection ◽  
Therapeutic Angiogenesis ◽  
Adipose Derived Stem Cells ◽  
Poly Ethylene Glycol ◽  
Transfection Efficacy ◽  
Stromal Cell Derived Factor ◽  
Poly Ethylene

Poly (ethylene glycol) hydrogel scaffolds with multiscale porosity for culture of human adipose-derived stem cells

2019 ◽  
Vol 30 (11) ◽  
pp. 895-918 ◽  
Author(s):  
Haley H. Barnett ◽  
Abitha M. Heimbuck ◽  
India Pursell ◽  
Rachel A. Hegab ◽  
Benjamin J. Sawyer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ethylene Glycol ◽  
Adipose Derived Stem Cells ◽  
Poly Ethylene Glycol ◽  
Hydrogel Scaffolds ◽  
Poly Ethylene

Structural engineering to control density, conformation, and bioactivity of the poly(ethylene glycol)-grafted poly(urethane urea) scaffolds

2018 ◽  
Vol 34 (2) ◽  
pp. 209-223
Author(s):  
Shideh Shaneh ◽  
Fatemeh Shokrolahi ◽  
Parvin Shokrollahi ◽  
Hamid Yeganeh ◽  
Hossein Omidian
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethylene Glycol ◽  
Structural Engineering ◽  
Cell Attachment ◽  
Poly Ethylene Glycol ◽  
Salt Leaching ◽  
The Matrix ◽  
Diphenyl Tetrazolium Bromide ◽  
Poly Ethylene

Poly(urethane urea) scaffolds were fabricated through combined salt leaching and solvent casting methods. The scaffolds were then functionalized via aminolysis with poly(ethylene glycol) (PEG- g-PUU). To compare its bioactivity, gelatin was also grafted onto the aminolyzed poly(urethane urea) surface (Gel- g-PUU). Chemical changes at the surface were then monitored using quantitative/qualitative methods. Grafting with both gelatin and poly(ethylene glycol) remarkably enhanced the wettability of poly(urethane urea). Proliferation of human adipose–derived mesenchymal stem cells on poly(urethane urea) and the modified poly(urethane urea)s was evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide assay. The cell experiment results showed that both the modified poly(urethane urea)s enhanced the attachment and proliferation of human adipose–derived mesenchymal stem cells compared to pure poly(urethane urea). Based on previous reports, while a supportive role is observed at adequate poly(ethylene glycol) graft densities, cell adhesion and proliferation are inhibited at very high grafting densities. To correlate the cell data to poly(ethylene glycol) conformations, the surface tension was measured. Data on human adipose–derived mesenchymal stem cells’ attachment/proliferation and contact angle/surface free energy together showed that the grafting density of poly(ethylene glycol) was regulated by optimizing aminolysis conditions, careful selection of poly(ethylene glycol)’s molecular weight, and bulk properties of the matrix poly(urethane urea). As a result, surface overcrowding and brush conformation of the poly(ethylene glycol) chains were avoided, and human adipose–derived mesenchymal stem cell attachment and proliferation occurred on the PEG- g-PUU scaffold at a comparable level to the Gel- g-PUU.

scholarly journals Poly(3-hydroxybutyrate)/poly(ethylene glycol) scaffolds with different microstructure: the effect on growth of mesenchymal stem cells

3 Biotech ◽  
2018 ◽  
Vol 8 (8) ◽  
Author(s):  
A. P. Bonartsev ◽  
I. I. Zharkova ◽  
V. V. Voinova ◽  
E. S. Kuznetsova ◽  
V. A. Zhuikov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene ◽  
Effect On Growth

scholarly journals Osteogenic differentiation of human mesenchymal stem cells on poly(ethylene glycol)-variant biomaterials

2009 ◽  
Vol 91A (4) ◽  
pp. 975-984 ◽  
Author(s):  
Tonye Briggs ◽  
Matthew D. Treiser ◽  
Paul F. Holmes ◽  
Joachim Kohn ◽  
Prabhas V. Moghe ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Ethylene Glycol ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Label-Free Quantification Proteomics for the Identification of Mesenchymal Stromal Cell Matrisome Inside 3D Poly(Ethylene Glycol) Hydrogels

2018 ◽  
Vol 7 (21) ◽  
pp. 1800534 ◽  
Author(s):  
Yannick R. Devaud ◽  
Eva Avilla-Royo ◽  
Christian Trachsel ◽  
Jonas Grossmann ◽  
Ivan Martin ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Mesenchymal Stromal Cell ◽  
Stromal Cell ◽  
Label Free ◽  
Poly Ethylene Glycol ◽  
Label Free Quantification ◽  
Poly Ethylene ◽  
Free Quantification
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