scholarly journals Hypoxia Is a Critical Parameter for Chondrogenic Differentiation of Human Umbilical Cord Blood Mesenchymal Stem Cells in Type I/III Collagen Sponges

2017 ◽  
Vol 18 (9) ◽  
pp. 1933 ◽  
Author(s):  
Tangni Gómez-Leduc ◽  
Mélanie Desancé ◽  
Magalie Hervieu ◽  
Florence Legendre ◽  
David Ollitrault ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Chondrogenic Differentiation ◽  
Critical Parameter ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Type I

scholarly journals Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Contribute to Chondrogenesis in Coculture with Chondrocytes

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Xingfu Li ◽  
Li Duan ◽  
Yujie Liang ◽  
Weimin Zhu ◽  
Jianyi Xiong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Protein Expression ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Type I ◽  
Low Density

Human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) have been shown as the most potential stem cell source for articular cartilage repair. In this study, we aimed to develop a method for long-term coculture of human articular chondrocytes (hACs) and hUCB-MSCs at low density in vitro to determine if the low density of hACs could enhance the hUCB-MSC chondrogenic differentiation as well as to determine the optimal ratio of the two cell types. Also, we compared the difference between direct coculture and indirect coculture at low density. Monolayer cultures of hUCB-MSCs and hACs were investigated at different ratios, at direct cell-cell contact groups for 21 days. Compared to direct coculture, hUCB-MSCs and hACs indirect contact culture significantly increased type II collagen (COL2) and decreased type I collagen (COL1) protein expression levels. SRY-box 9 (SOX9) mRNA levels and protein expression were highest in indirect coculture. Overall, these results indicate that low density direct coculture induces fibrocartilage. However, indirect coculture in conditioned chondrocyte cell culture medium can increase expression of chondrogenic markers and induce hUCB-MSCs differentiation into mature chondrocytes. This work demonstrates that it is possible to promote chondrogenesis of hUCB-MSCs in combination with hACs, further supporting the concept of novel coculture strategies for tissue engineering.

Chondrogenic differentiation of human umbilical cord blood-derived mesenchymal stem cells in vitro

2015 ◽  
Vol 78 (8) ◽  
pp. 667-675 ◽  
Author(s):  
Azza Mostafa Ibrahim ◽  
Nesrine Mohamed Elgharabawi ◽  
Manal Mohamed Makhlouf ◽  
Omnia Yahia Ibrahim
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Chondrogenic Differentiation ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord

scholarly journals Thrombospondin-2 secreted by human umbilical cord blood-derived mesenchymal stem cells promotes chondrogenic differentiation

Stem Cells ◽  
2013 ◽  
Vol 31 (10) ◽  
pp. 2136-2148 ◽  
Author(s):  
Sang Young Jeong ◽  
Dong Hyun Kim ◽  
Jueun Ha ◽  
Hye Jin Jin ◽  
Soon-Jae Kwon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Chondrogenic Differentiation ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord
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