scholarly journals Enhanced Cell Growth of Adipocyte-Derived Mesenchymal Stem Cells Using Chemically-Defined Serum-Free Media

2017 ◽  
Vol 18 (8) ◽  
pp. 1779 ◽  
Author(s):  
Myung-Suk Lee ◽  
Christine Youn ◽  
Jeong Kim ◽  
Byoung Park ◽  
Jongchan Ahn ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Growth ◽  
Serum Free ◽  
Free Media

scholarly journals Ex VivoExpansion of Human Mesenchymal Stem Cells in Defined Serum-Free Media

2012 ◽  
Vol 2012 ◽  
pp. 1-21 ◽  
Author(s):  
Sunghoon Jung ◽  
Krishna M. Panchalingam ◽  
Lawrence Rosenberg ◽  
Leo A. Behie
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Therapeutic Potential ◽  
Culture Media ◽  
Human Mesenchymal Stem Cells ◽  
Efficient Production ◽  
Serum Free ◽  
Medium Development ◽  
Current Cell ◽  
Free Media

Human mesenchymal stem cells (hMSCs) are presently being evaluated for their therapeutic potential in clinical studies to treat various diseases, disorders, and injuries. To date, early-phase studies have indicated that the use of both autologous and allogeneic hMSCs appear to be safe; however, efficacy has not been demonstrated in recent late-stage clinical trials. Optimized cell bioprocessing protocols may enhance the efficacy as well as safety of hMSC therapeutics. Classical media used for generating hMSCs are typically supplemented with ill-defined supplements such as fetal bovine serum (FBS) or human-sourced alternatives. Ideally, culture media are desired to have well-defined serum-free formulations that support the efficient production of hMSCs while maintaining their therapeutic and differentiation capacity. Towards this objective, we review here current cell culture media for hMSCs and discuss medium development strategies.

Isolation and large-scale expansion of bone marrow-derived mesenchymal stem cells with serum-free media under GMP-compliance

Cytotherapy ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. S111 ◽  
Author(s):  
S.H. Mei ◽  
M. Salkhordeh ◽  
F. Xue ◽  
J. Zhang ◽  
I. Watpool ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Large Scale ◽  
Serum Free ◽  
Scale Expansion ◽  
Free Media

scholarly journals Platelet lysate as a novel serum-free media supplement for the culture of equine bone marrow-derived mesenchymal stem cells

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Maria C. Naskou ◽  
Scarlett M. Sumner ◽  
Anna Chocallo ◽  
Hannah Kemelmakher ◽  
Merrilee Thoresen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Platelet Lysate ◽  
Serum Free ◽  
Free Media ◽  
Equine Bone

Long-term culture of human umbilical cord mesenchymal stem cells in defined serum free media

Cytotherapy ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. S70
Author(s):  
Z. Han ◽  
Y. Wang ◽  
Y. Chi ◽  
S. Yan ◽  
A. Mao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Human Umbilical Cord ◽  
Serum Free ◽  
Long Term Culture ◽  
Free Media

scholarly journals Evaluation of characteristic of human turbinate derived mesenchymal stem cells cultured in the serum free media

PLoS ONE ◽  
2017 ◽  
Vol 12 (10) ◽  
pp. e0186249 ◽  
Author(s):  
Se Hwan Hwang ◽  
WeonSun Lee ◽  
Sang Hi Park ◽  
Hee Jin Lee ◽  
Sun Hwa Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Serum Free ◽  
Free Media ◽  
Cells Cultured

scholarly journals Canine and Equine Mesenchymal Stem Cells Grown in Serum Free Media Have Altered Immunophenotype

2015 ◽  
Vol 12 (2) ◽  
pp. 245-256 ◽  
Author(s):  
Kaitlin C. Clark ◽  
Amir Kol ◽  
Salpi Shahbenderian ◽  
Jennifer L. Granick ◽  
Naomi J. Walker ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Serum Free ◽  
Free Media

scholarly journals Comparative Analysis of Mesenchymal Stem Cells Cultivated in Serum Free Media

2022 ◽  
Author(s):  
Joo Youn Lee ◽  
Min Hee Kang ◽  
Ji Eun Jang ◽  
Jeong Eon Lee ◽  
Yuyeong Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Therapy ◽  
Mass Production ◽  
Differential Expression Analysis ◽  
Population Doubling ◽  
Animal Origin ◽  
Population Doubling Time ◽  
Serum Free ◽  
Free Media

Abstract Stem cells are attractive candidates for the regeneration of tissue and organ. Mesenchymal stem cells (MSCs) have been extensively investigated for their potential applications in regenerative medicine and cell therapy. For developing effective stem cell therapy, the mass production of consistent quality cells is required. The cell culture medium is the most critical aspect of the mass production of qualified stem cells. Classically, fetal bovine serum (FBS) has been used as a culture supplement for MSCs. Due to the undefined and heterologous composition of animal origin components in FBS, efforts to replace animal-derived components with non-animal-derived substances led to safe serum free media (SFM). Adipose derived mesenchymal stem cells (ADSCs) cultivated in SFM provided a more stable population doubling time (PDT) to later passage and more cells in a shorter time compared to FBS containing media. ADSCs cultivated in SFM had lower cellular senescence, lower immunogenicity, and higher genetic stability than ADSCs cultivated in FBS containing media. Differential expression analysis of mRNAs and proteins showed that the expression of genes related with apoptosis, immune response, and inflammatory response were significantly up-regulated in ADSCs cultivated in FBS containing media. ADSCs cultivated in SFM showed similar therapeutic efficacy in an acute pancreatitis mouse model to ADSCs cultivated in FBS containing media. Consideration of clinical trials, not only pre-clinical trial, suggests that cultivation of MSCs using SFM might offer more safe cell therapeutics as well as repeated administration due to low immunogenicity.

scholarly journals Serum-free media for mesenchymal stem cells expansion on microcarriers

2015 ◽  
Vol 9 (Suppl 9) ◽  
pp. P70 ◽  
Author(s):  
Céline Martin ◽  
Alexandre Piccini ◽  
Isabelle Chevalot ◽  
Eric Olmos ◽  
Emmanuel Guedon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Serum Free ◽  
Free Media

Defined serum-free media for in vitro expansion of adipose-derived mesenchymal stem cells

Cytotherapy ◽  
2014 ◽  
Vol 16 (7) ◽  
pp. 915-926 ◽  
Author(s):  
Shahla Hamza Al-Saqi ◽  
Mohammed Saliem ◽  
Suvi Asikainen ◽  
Hernan Concha Quezada ◽  
Åsa Ekblad ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Serum Free ◽  
In Vitro Expansion ◽  
Free Media
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