scholarly journals Conditioned Medium from Malignant Breast Cancer Cells Induces an EMT-Like Phenotype and an Altered N-Glycan Profile in Normal Epithelial MCF10A Cells

2017 ◽  
Vol 18 (8) ◽  
pp. 1528 ◽  
Author(s):  
Jia Guo ◽  
Changmei Liu ◽  
Xiaoman Zhou ◽  
Xiaoqiang Xu ◽  
Linhong Deng ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Conditioned Medium ◽  
Breast Cancer Cells ◽  
Glycan Profile ◽  
Malignant Breast ◽  
Mcf10a Cells

scholarly journals Epinephrine Infiltration of Adipose Tissue Impacts MCF7 Breast Cancer Cells and Total Lipid Content

2019 ◽  
Vol 20 (22) ◽  
pp. 5626 ◽  
Author(s):  
Pierre Avril ◽  
Luciano Vidal ◽  
Sophie Barille-Nion ◽  
Louis-Romée Le Nail ◽  
Françoise Redini ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Adipose Tissue ◽  
Tumor Progression ◽  
Cancer Cells ◽  
Conditioned Medium ◽  
Lipid Composition ◽  
Breast Cancer Cells ◽  
Quiescent State ◽  
Mcf7 Cells ◽  
Mesenchymal Transition

Background: Considering the positive or negative potential effects of adipocytes, depending on their lipid composition, on breast tumor progression, it is important to evaluate whether adipose tissue (AT) harvesting procedures, including epinephrine infiltration, may influence breast cancer progression. Methods: Culture medium conditioned with epinephrine-infiltrated adipose tissue was tested on human Michigan Cancer Foundation-7 (MCF7) breast cancer cells, cultured in monolayer or in oncospheres. Lipid composition was evaluated depending on epinephrine-infiltration for five patients. Epinephrine-infiltrated adipose tissue (EI-AT) or corresponding conditioned medium (EI-CM) were injected into orthotopic breast carcinoma induced in athymic mouse. Results: EI-CM significantly increased the proliferation rate of MCF7 cells Moreover EI-CM induced an output of the quiescent state of MCF7 cells, but it could be either an activator or inhibitor of the epithelial mesenchymal transition as indicated by gene expression changes. EI-CM presented a significantly higher lipid total weight compared with the conditioned medium obtained from non-infiltrated-AT of paired-patients. In vivo, neither the EI-CM or EI-AT injection significantly promoted MCF7-induced tumor growth. Conclusions: Even though conditioned media are widely used to mimic the secretome of cells or tissues, they may produce different effects on tumor progression, which may explain some of the discrepancy observed between in vitro, preclinical and clinical data using AT samples.

Extracellular vesicles from MDA-MB-231 breast cancer cells stimulated with linoleic acid promote an EMT-like process in MCF10A cells

2014 ◽  
Vol 91 (6) ◽  
pp. 299-310 ◽  
Author(s):  
Octavio Galindo-Hernandez ◽  
Nathalia Serna-Marquez ◽  
Rocio Castillo-Sanchez ◽  
Eduardo Perez Salazar
Keyword(s):  
Breast Cancer ◽  
Linoleic Acid ◽  
Cancer Cells ◽  
Extracellular Vesicles ◽  
Breast Cancer Cells ◽  
Mcf10a Cells

scholarly journals Osteoblast-conditioned medium promotes proliferation and sensitizes breast cancer cells to imatinib treatment

2007 ◽  
Vol 14 (1) ◽  
pp. 61-72 ◽  
Author(s):  
Marina Brama ◽  
Sabrina Basciani ◽  
Sara Cherubini ◽  
Stefania Mariani ◽  
Silvia Migliaccio ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tyrosine Kinase ◽  
Cancer Cells ◽  
Conditioned Medium ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Breast Cancer Cell Lines ◽  
Imatinib Treatment ◽  
Human Breast ◽  
Mcf 7

Inhibition of platelet-derived growth factor receptor (PDGFR) signaling restricts the growth of human breast cancer in the bone of nude mice. We hypothesized that osteoblast-secreted substances may alter the response capacity of breast cancer cells to the PDGFRs tyrosine kinase inhibitor imatinib mesylate. We found that osteoblast-conditioned medium (OCM) increases the proliferation rate of the estrogen receptor negative (ER−) MDA-MB-231 and of the ER+ MCF-7 human breast cancer cell lines and the growth-promoting effect on ER+ cells is independent from estrogen. OCM significantly improved the dose- and the time-dependent sensitivity of the tumor cells to the anti-proliferative effect of imatinib. We also found that MDA-MB-231 and MCF-7 cells express the two PDGFRs subtypes, PDGFR-α and PDGFR-β, and OCM treatment increases the expression of the PDGFRs. Furthermore, imatinib inhibited the phosphorylation rate of its target tyrosine kinase receptors. We conclude that bone microenvironment, through osteoblast-secreted substances may cause estrogen-independent proliferation of breast cancer cells by a mechanism mediated by the induction of PDGFRs expression. The enhanced sensitivity of OCM-treated breast cancer cells to imatinib would justify investigation on the efficacy of imatinib in bone breast cancer metastasis.

An IgG human monoclonal antibody reactive with a surface membrane antigen expressed on malignant breast cancer cells

1991 ◽  
Vol 2 (2) ◽  
pp. 74-83 ◽  
Author(s):  
Marshall R. Posner ◽  
Hillary S. Elboim ◽  
Marea B. Tumber ◽  
Peter M. Wiest ◽  
Lance M. Tibbetts
Keyword(s):  
Breast Cancer ◽  
Monoclonal Antibody ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Human Monoclonal Antibody ◽  
Surface Membrane ◽  
Malignant Breast

Effect of polyclonal activators on cytokine production by blood cells and by malignant breast cancer cells

2016 ◽  
Vol 466 (1) ◽  
pp. 45-47 ◽  
Author(s):  
T. A. Kunts ◽  
K. V. Karpukhina ◽  
E. S. Mikhaylova ◽  
I. O. Marinkin ◽  
N. A. Varaksin ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Blood Cells ◽  
Breast Cancer Cells ◽  
Cytokine Production ◽  
Malignant Breast
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