scholarly journals Hepatoprotective Effect of Aqueous Extract from the Seeds of Orychophragmus violaceus against Liver Injury in Mice and HepG2 Cells

2017 ◽  
Vol 18 (6) ◽  
pp. 1197 ◽  
Author(s):  
Xiaowei Huo ◽  
Chenqi Liu ◽  
Li Gao ◽  
Xudong Xu ◽  
Nailiang Zhu ◽  
...  
Keyword(s):  
Liver Injury ◽  
Aqueous Extract ◽  
Hepg2 Cells ◽  
Hepatoprotective Effect ◽  
Orychophragmus Violaceus

scholarly journals Bamboo Stems (Phyllostachys nigra variety henosis) Containing Polyphenol Mixtures Activate Nrf2 and Attenuate Phenylhydrazine-Induced Oxidative Stress and Liver Injury

Nutrients ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 114 ◽  
Author(s):  
Ji Hye Yang ◽  
Moon-Hee Choi ◽  
Chang-Su Na ◽  
Sam Seok Cho ◽  
Jae Hoon Kim ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Liver Injury ◽  
Iron Overload ◽  
Liver Damage ◽  
Hepg2 Cells ◽  
Hepatoprotective Effect ◽  
Glutathione Depletion ◽  
Related Factor ◽  
Nrf2 Target Gene ◽  
Phyllostachys Nigra

This study was designed to investigate the hepatoprotective effect of bamboo stems using in vitro and in vivo experimental liver damage models. Ethyl acetate fraction of 80% ethanol extract of Phyllostachys nigra stem (PN3) containing polyphenols had a higher NQO1-ARE reporter gene activity as monitored by the activity of the NF-E2-related factor (Nrf2) antioxidant pathway in cells in comparison to extracts from other species and under other conditions. The Nrf2 was translocated from the cytosol to the nucleus in response to PN3, followed by induction of the Nrf2 target gene expression, including HO-1, GCL, and NQO-1 in HepG2 cells. Phosphorylation of Nrf2 in HepG2 cells was enhanced in PN3, which was mediated by PKCδ, ERK, and p38 MAPK. Consequently, PN3 inhibited arachidonic acid (AA) + iron-induced reactive oxygen species generation and glutathione depletion, and, thus, highlighted their role in cytotoxicity. Treatment with major polyphenols of PN3, including catechin, chlorogenic acid, caffeic acid, and p-coumaric acid, also improved AA + iron-mediated oxidative stress and, thus, improved cell viability. Treatment with phenylhydrazine in mice, i.e., the iron overload liver injury model, increased plasma alanine aminotransferase and aspartate aminotransferase levels and changed histological features in mice—a response that was almost completely blocked by PN3 administration. Moreover, PN3 extract mitigated phenylhydrazine-induced oxidative stress and inflammatory responses. Conclusively, PN3 can exert a hepatoprotective effect against iron overload-induced acute liver damage due to its antioxidant properties.

scholarly journals Hepatoprotective effect of aqueous extract of Melothria perpusilla against carbon tetrachloride induced liver injury in albino rats

2017 ◽  
Vol 5 (3) ◽  
pp. 806 ◽  
Author(s):  
Nokul S. Yengkhom ◽  
Ngangom Gunindro ◽  
Sania M. Kholi ◽  
Rakesh S. Moirangthem ◽  
Bharati D. Rajkumari
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Aqueous Extract ◽  
Liquid Paraffin ◽  
Herbal Medicines ◽  
Modern Medicine ◽  
Hepatoprotective Effect ◽  
Albino Rats ◽  
Group Iii ◽  
Group I

Background: In absence of reliable hepatoprotective drug in modern medicine, the traditional herbal medicines have been emphasized. Present study was designed to assess hepatoprotective effect of aqueous extract of Melothria perpusilla (AEMP) against carbon tetrachloride (CCl4) induced liver injury.Methods: Five groups of animals with 6 rats in each were treated for 7 days.  Group I received 1% gum acacia in distilled water (1 ml/200 g p.o.) daily.  Group II, III, IV and V received CCl4 in liquid paraffin (1 ml/kg s.c.) on day 2, 4 and 6. Group III, IV and V were treated respectively with silymarin (100 mg/kg p.o.), AEMP- 200 and 400 mg/kg p.o. daily. On day 8, liver injury was assessed by measuring serum ALT, AST, ALP and bilirubin.Results: ALT, AST, ALP and bilirubin were significantly reduced in groups receiving both CCl4 and AEMP when compared with CCl4 treated group.Conclusions: AEMP produced hepatoprotective effect against CCl4 induced liver injury.

Hepatoprotective effect of esculetin on ethanol-induced liver injury in human HepG2 cells and C57BL/6J mice

2018 ◽  
Vol 40 ◽  
pp. 536-543 ◽  
Author(s):  
Jaemin Lee ◽  
Jinwoo Yang ◽  
Jiyoung Jeon ◽  
Heon Sang Jeong ◽  
Junsoo Lee ◽  
...  
Keyword(s):  
Liver Injury ◽  
Hepg2 Cells ◽  
Hepatoprotective Effect

scholarly journals Lactobacillus acidophilus LA5 improves saturated fat-induced obesity mouse model through the enhanced intestinal Akkermansia muciniphila

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Thunnicha Ondee ◽  
Krit Pongpirul ◽  
Peerapat Visitchanakun ◽  
Wilasinee Saisorn ◽  
Suthicha Kanacharoen ◽  
...  
Keyword(s):  
Liver Injury ◽  
Lactobacillus Acidophilus ◽  
Hepg2 Cells ◽  
Saturated Fat ◽  
Liver Weight ◽  
Gram Negative ◽  
Fecal Microbiome ◽  
Akkermansia Muciniphila ◽  
Anti Inflammatory ◽  
Inflammatory Molecules

AbstractObesity, a major healthcare problem worldwide, induces metabolic endotoxemia through the gut translocation of lipopolysaccharides (LPS), a major cell wall component of Gram-negative bacteria, causing a chronic inflammatory state. A combination of several probiotics including Lactobacillus acidophilus 5 (LA5), a potent lactic acid-producing bacterium, has previously been shown to attenuate obesity. However, data on the correlation between a single administration of LA5 versus microbiota alteration might be helpful for the probiotic adjustment. LA5 was administered daily together with a high-fat diet (HFD) for 8 weeks in mice. Furthermore, the condition media of LA5 was also tested in a hepatocyte cell-line (HepG2 cells). Accordingly, LA5 attenuated obesity in mice as demonstrated by weight reduction, regional fat accumulation, lipidemia, liver injury (liver weight, lipid compositions, and liver enzyme), gut permeability defect, endotoxemia, and serum cytokines. Unsurprisingly, LA5 improved these parameters and acidified fecal pH leads to the attenuation of fecal dysbiosis. The fecal microbiome analysis in obese mice with or without LA5 indicated; (i) decreased Bacteroidetes (Gram-negative anaerobes that predominate in non-healthy conditions), (ii) reduced total fecal Gram-negative bacterial burdens (the sources of gut LPS), (iii) enhanced Firmicutes (Gram-positive bacteria with potential benefits) and (iv) increased Verrucomycobia, especially Akkermansia muciniphila, a bacterium with the anti-obesity property. With LA5 administration, A. muciniphila in the colon were more than 2,000 folds higher than the regular diet mice as determined by 16S rRNA. Besides, LA5 produced anti-inflammatory molecules with a similar molecular weight to LPS that reduced cytokine production in LPS-activated HepG2 cells. In conclusion, LA5 attenuated obesity through (i) gut dysbiosis attenuation, partly through the promotion of A. muciniphila (probiotics with the difficulty in preparation processes), (ii) reduced endotoxemia, and (iii) possibly decreased liver injury by producing the anti-inflammatory molecules.

Hepatoprotective effect of selected isoandrographolide derivatives on steatotic HepG2 cells and High Fat Diet fed rats

2021 ◽  
Vol 899 ◽  
pp. 174056
Author(s):  
Erenius Toppo ◽  
Naif Abdullah Al-Dhabi ◽  
Chinnakulandai Sankar ◽  
Sanmugapriya Nagul Kumar ◽  
Kanniappan Buvanesvaragurunathan ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Hepg2 Cells ◽  
Hepatoprotective Effect ◽  
High Fat

Hepatoprotective effect of a curcumin/absinthium compound in experimental severe liver injury

2003 ◽  
Vol 4 (3) ◽  
pp. 122-127 ◽  
Author(s):  
F MAROTTA ◽  
YR SHIELD ◽  
T BAMBA ◽  
Y NAITO ◽  
E MINELLI ◽  
...  
Keyword(s):  
Liver Injury ◽  
Hepatoprotective Effect ◽  
Severe Liver

Hepatoprotective effect of coffee pulp aqueous extract combined with simvastatin against hepatic steatosis in high-fat diet-induced obese rats

2019 ◽  
Vol 54 ◽  
pp. 568-577 ◽  
Author(s):  
Atcharaporn Ontawong ◽  
Oranit Boonphang ◽  
Tipthida Pasachan ◽  
Acharaporn Duangjai ◽  
Anchalee Pongchaidecha ◽  
...  
Keyword(s):  
Hepatic Steatosis ◽  
Aqueous Extract ◽  
High Fat Diet ◽  
Hepatoprotective Effect ◽  
High Fat ◽  
Coffee Pulp ◽  
Obese Rats
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