scholarly journals Differential Proteome Analysis of a Flor Yeast Strain under Biofilm Formation

2017 ◽  
Vol 18 (4) ◽  
pp. 720 ◽  
Author(s):  
Jaime Moreno-García ◽  
Juan Mauricio ◽  
Juan Moreno ◽  
Teresa García-Martínez
Keyword(s):  
Biofilm Formation ◽  
Yeast Strain ◽  
Proteome Analysis ◽  
Flor Yeast

Stress responsive proteins of a flor yeast strain during the early stages of biofilm formation

2016 ◽  
Vol 51 (5) ◽  
pp. 578-588 ◽  
Author(s):  
Jaime Moreno-García ◽  
Juan Carlos Mauricio ◽  
Juan Moreno ◽  
Teresa García-Martínez
Keyword(s):  
Biofilm Formation ◽  
Yeast Strain ◽  
Early Stages ◽  
Flor Yeast

scholarly journals High Foam Phenotypic Diversity and Variability in Flocculant Gene Observed for Various Yeast Cell Surfaces Present as Industrial Contaminants

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 127
Author(s):  
Catarina M. de Figueiredo ◽  
Daniella H. Hock ◽  
Débora Trichez ◽  
Maria de Lourdes B. Magalhães ◽  
Mario L. Lopes ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Yeast Strain ◽  
Phenotypic Diversity ◽  
Strain Engineering ◽  
Fuel Ethanol ◽  
Invasive Growth ◽  
Foam Formation ◽  
Yeast Strains ◽  
Length Polymorphisms ◽  
Contaminant Yeast

Many contaminant yeast strains that survive inside fuel ethanol industrial vats show detrimental cell surface phenotypes. These harmful effects may include filamentation, invasive growth, flocculation, biofilm formation, and excessive foam production. Previous studies have linked some of these phenotypes to the expression of FLO genes, and the presence of gene length polymorphisms causing the expansion of FLO gene size appears to result in stronger flocculation and biofilm formation phenotypes. We performed here a molecular analysis of FLO1 and FLO11 gene polymorphisms present in contaminant strains of Saccharomyces cerevisiae from Brazilian fuel ethanol distilleries showing vigorous foaming phenotypes during fermentation. The size variability of these genes was correlated with cellular hydrophobicity, flocculation, and highly foaming phenotypes in these yeast strains. Our results also showed that deleting the primary activator of FLO genes (the FLO8 gene) from the genome of a contaminant and highly foaming industrial strain avoids complex foam formation, flocculation, invasive growth, and biofilm production by the engineered (flo8∆::BleR/flo8Δ::kanMX) yeast strain. Thus, the characterization of highly foaming yeasts and the influence of FLO8 in this phenotype open new perspectives for yeast strain engineering and optimization in the sugarcane fuel-ethanol industry.

Use of a flor yeast strain for the second fermentation of sparkling wines: Effect of endogenous CO2 over-pressure on the volatilome

2020 ◽  
Vol 308 ◽  
pp. 125555 ◽  
Author(s):  
Rafael Martínez-García ◽  
Yenifer Roldán-Romero ◽  
Juan Moreno ◽  
Anna Puig-Pujol ◽  
Juan Carlos Mauricio ◽  
...  
Keyword(s):  
Yeast Strain ◽  
Flor Yeast

scholarly journals Btn2p is involved in ethanol tolerance and biofilm formation in flor yeast

2008 ◽  
Vol 8 (7) ◽  
pp. 1127-1136 ◽  
Author(s):  
Marisa Espinazo-Romeu ◽  
Jesús M. Cantoral ◽  
Emilia Matallana ◽  
Agustín Aranda
Keyword(s):  
Biofilm Formation ◽  
Ethanol Tolerance ◽  
Flor Yeast

scholarly journals Impact of Moderate Temperature Changes on Neisseria meningitidis Adhesion Phenotypes and Proteome

2016 ◽  
Vol 84 (12) ◽  
pp. 3484-3495 ◽  
Author(s):  
Martin Lappann ◽  
Andreas Otto ◽  
Madita Brauer ◽  
Dörte Becher ◽  
Ulrich Vogel ◽  
...  
Keyword(s):  
Protein Expression ◽  
Neisseria Meningitidis ◽  
Outer Membrane ◽  
Biofilm Formation ◽  
Proteome Analysis ◽  
Ambient Air ◽  
Temperature Dependent ◽  
Content Type ◽  
Temperature Changes

Neisseria meningitidis , the meningococcus, bears the potential to cause life-threatening invasive diseases, but it usually colonizes the nasopharynx without causing any symptoms. Within the nasopharynx, Neisseria meningitidis must face temperature changes depending on the ambient air temperature. Indeed, the nasopharyngeal temperature can be substantially lower than 37°C, the temperature commonly used in experimental settings. Here, we compared the levels of meningococcal biofilm formation, autoaggregation, and cellular adherence at 32°C and 37°C and found a clear increase in all these phenotypes at 32°C suggestive of a stronger in vivo colonization capability at this temperature. A comparative proteome analysis approach revealed differential protein expression levels between 32°C and 37°C, predominantly affecting the bacterial envelope. A total of 375 proteins were detected. Use of database annotation or the PSORTb algorithm predicted 49 of those proteins to be localized in the outer membrane, 21 in either the inner or outer membrane, 35 in the periplasm, 56 in the inner membrane, and 208 in the cytosol; for 6 proteins, no annotation or prediction was available. Temperature-dependent regulation of protein expression was seen particularly in the periplasm as well as in the outer and inner membranes. N eisserial h eparin b inding a ntigen (NHBA), NMB1030, and adhesin complex protein (ACP) showed the strongest upregulation at 32°C and were partially responsible for the observed temperature-dependent phenotypes. Screening of different global regulators of Neisseria meningitidis suggested that the extracytoplasmic sigma factor σ E might be involved in temperature-dependent biofilm formation. In conclusion, subtle temperature changes trigger adaptation events promoting mucosal colonization by meningococci.

scholarly journals Cell Surface Phenotypic Diversity and Flocculation Gene Variability in Contaminant Industrial Fuel-Ethanol Yeast Strains Exhibiting Highly Foaming Phenotypes

2021 ◽  
Author(s):  
Catarina M. de Figueiredo ◽  
Daniella H. Hock ◽  
Debora Trichez ◽  
Maria de Lurdes B. Magalhães ◽  
Mario L. Lopes ◽  
...  
Keyword(s):  
Cell Surface ◽  
Biofilm Formation ◽  
Yeast Strain ◽  
Phenotypic Diversity ◽  
Strain Engineering ◽  
Fuel Ethanol ◽  
Invasive Growth ◽  
Foam Formation ◽  
Yeast Strains ◽  
Gene Variability

Many contaminant yeast strains able to survive inside fuel ethanol industrial vats show detrimental cell surface phenotypes, such as filamentation, invasive growth, flocculation, biofilm formation and excessive foam production. Previous studies have linked some of these phenotypes to the expression of FLO genes, and the presence of gene length polymorphisms causing the expansion of FLO gene size appears to result in stronger flocculation and biofilm formation phenotypes. We have performed here a molecular analysis of FLO1 and FLO11 gene polymorphisms present in contaminant strains of S. cerevisae from Brazilian fuel ethanol distilleries showing strong foaming phenotypes during fermentation. The size variability of these genes was correlated with cellular hydrophobicity, flocculation and highly foaming phenotypes in these yeast strains. Our results also show that deleting the major activator of FLO genes (the FLO8 gene) from the genome of a contaminant and highly foaming industrial strain avoids problematic foam formation, flocculation, invasive growth and biofilm production by the engineered (flo8∆::BleR / flo8Δ::kanMX) yeast strain. Thus, the characterization of highly foaming yeasts and the influence of FLO8 in this phenotype opens new perspectives for yeast strain engineering and optimization in the sugarcane fuel-ethanol industry.

Bacteria and biofilms under the influence of shear

2020 ◽  
Author(s):  
Christine Müller-Renno ◽  
Jürgen Seehase ◽  
Katharina Huttenlochner ◽  
Jonas Chodorski ◽  
Roland Ulber ◽  
...  
Keyword(s):  
Surface Energy ◽  
Biofilm Formation ◽  
Proteome Analysis ◽  
Lateral Force ◽  
Adherent Cell ◽  
Adhesion Forces ◽  
Shear Forces ◽  
Flowing Liquid ◽  
Force Microscopy ◽  
Scanning Force

<p>In our attempts to improve the biofilm formation for productive bacteria (here the gram-negative seawater bacterium Paracoccus seriniphilus), we focus on the attachment of single bacteria to a solid surface as the first step of the biofilm formation process. Beside adhesion forces and elasticity of the bacteria, we investigate the minimal detachment forces due to lateral shear forces.</p> <p>In order to investigate the influence of shear forces on already adhered bacteria in the laboratory, the Lateral Force Microscopy(LFM) was used first.The tip is moved laterally towards the adherent cell with different lateral forces until the cell detaches and thus the force required to shear the cell is determined.</p> <p>By applying LFM, we found a correlation between the applied force and the number of moved bacteria as well as between the number of detached bacteria and the surface energy of the substrate. Further, any structuring of the substrate hinders the detachment substantially [1]. In agreement with the vertical adhesion forces, the bacteria are harder to detach at pH 4 than at pH 7.</p> <p>In order to get closer to reality, the next step is to examine the (lateral) scanning force microscopic measurements under the influence of a flowing liquid and compare them with the LFM measurements. In combination with digital holography and proteome analysis, a better understanding of biofilm formation under the influence of a flowing liquid is to be achieved.</p> <p>[1] K. Huttenlochner, N. Davoudi, C. Schlegel, M. Bohley, C. Müller-Renno, J. C. Aurich, R. Ulber, and C. Ziegle "Paracoccus seriniphilus adhered on surfaces: Resistance of a seawater bacterium against shear forces under the influence of roughness, surface energy, and zeta potential of the surfaces." Biointerphases 13.5 (2018)</p>

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