scholarly journals An In Silico Identification of Common Putative Vaccine Candidates against Treponema pallidum: A Reverse Vaccinology and Subtractive Genomics Based Approach

2017 ◽  
Vol 18 (2) ◽  
pp. 402 ◽  
Author(s):  
Arun Kumar Jaiswal ◽  
Sandeep Tiwari ◽  
Syed Jamal ◽  
Debmalya Barh ◽  
Vasco Azevedo ◽  
...  
Keyword(s):  
In Silico ◽  
Treponema Pallidum ◽  
Reverse Vaccinology ◽  
Vaccine Candidates ◽  
Subtractive Genomics ◽  
In Silico Identification

In Silico Identification of Probable Drug and Vaccine Candidates Against Antibiotic-Resistant Acinetobacter baumannii

2020 ◽  
Vol 26 (5) ◽  
pp. 456-467
Author(s):  
Elaheh Zadeh Hosseingholi ◽  
Gholamreza Zarrini ◽  
Marayam Pashazadeh ◽  
Seyed Mohammad Gheibi Hayat ◽  
Ghader Molavi
Keyword(s):  
Acinetobacter Baumannii ◽  
In Silico ◽  
Antibiotic Resistant ◽  
Vaccine Candidates ◽  
In Silico Identification

scholarly journals Identification of vaccine and drug targets in Shigella dysenteriae sd197 using reverse vaccinology approach

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Khurshid Jalal ◽  
Tareq Abu-Izneid ◽  
Kanwal Khan ◽  
Muhammad Abbas ◽  
Ajmal Hayat ◽  
...  
Keyword(s):  
Drug Targets ◽  
Diarrheal Disease ◽  
Vaccine Candidate ◽  
Shigella Dysenteriae ◽  
Reverse Vaccinology ◽  
World Health ◽  
Vaccine Candidates ◽  
Subtractive Genomics ◽  
Health Organization ◽  
Chimeric Vaccine

AbstractShigellosis is characterized as diarrheal disease that causes a high mortality rate especially in children, elderly and immunocompromised patients. More recently, the World Health Organization advised safe vaccine designing against shigellosis due to the emergence of Shigella dysenteriae resistant strains. Therefore, the aim of this study is to identify novel drug targets as well as the design of the potential vaccine candidates and chimeric vaccine models against Shigella dysenteriae. A computational based Reverse Vaccinology along with subtractive genomics analysis is one of the robust approaches used for the prioritization of drug targets and vaccine candidates through direct screening of genome sequence assemblies. Herein, a successfully designed peptide-based novel highly antigenic chimeric vaccine candidate against Shigella dysenteriae sd197 strain is proposed. The study resulted in six epitopes from outer membrane WP_000188255.1 (Fe (3+) dicitrate transport protein FecA) that ultimately leads to the construction of twelve vaccine models. Moreover, V9 construct was found to be highly immunogenic, non-toxic, non-allergenic, highly antigenic, and most stable in terms of molecular docking and simulation studies against six HLAs and TLRS/MD complex. So far, this protein and multiepitope have never been characterized as vaccine targets against Shigella dysenteriae. The current study proposed that V9 could be a significant vaccine candidate against shigellosis and to ascertain that further experiments may be applied by the scientific community focused on shigellosis.

In silico identification of epitope-based vaccine candidates against HTLV-1

2021 ◽  
pp. 1-18
Author(s):  
Hamid Reza Jahantigh ◽  
Angela Stufano ◽  
Piero Lovreglio ◽  
Seyed Abdolrahim Rezaee ◽  
Khadijeh Ahmadi
Keyword(s):  
In Silico ◽  
Vaccine Candidates ◽  
In Silico Identification

scholarly journals In Silico Prediction of a Multitope Vaccine against Moraxella catarrhalis: Reverse Vaccinology and Immunoinformatics

Vaccines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 669
Author(s):  
Mohamed A. Soltan ◽  
Nada Elbassiouny ◽  
Helmy Gamal ◽  
Eslam B. Elkaeed ◽  
Refaat A. Eid ◽  
...  
Keyword(s):  
Immune Response ◽  
Outer Membrane ◽  
T Lymphocyte ◽  
In Silico ◽  
Moraxella Catarrhalis ◽  
In Silico Analysis ◽  
Reverse Vaccinology ◽  
Effective Vaccine ◽  
Vaccine Candidates ◽  
Tract Infections

Moraxella catarrhalis (M. catarrhalis) is a Gram-negative bacterium that can cause serious respiratory tract infections and middle ear infections in children and adults. M. catarrhalis has demonstrated an increasing rate of antibiotic resistance in the last few years, thus development of an effective vaccine is a major health priority. We report here a novel designed multitope vaccine based on the mapped epitopes of the vaccine candidates filtered out of the whole proteome of M. catarrhalis. After analysis of 1615 proteins using a reverse vaccinology approach, only two proteins (outer membrane protein assembly factor BamA and LPS assembly protein LptD) were nominated as potential vaccine candidates. These proteins were found to be essential, outer membrane, virulent and non-human homologs with appropriate molecular weight and high antigenicity score. For each protein, cytotoxic T lymphocyte (CTL), helper T lymphocyte (HTL) and B cell lymphocyte (BCL) epitopes were predicted and confirmed to be highly antigenic and cover conserved regions of the proteins. The mapped epitopes constituted the base of the designed multitope vaccine where suitable linkers were added to conjugate them. Additionally, beta defensin adjuvant and pan-HLA DR-binding epitope (PADRE) peptide were also incorporated into the construct to improve the stimulated immune response. The constructed multitope vaccine was analyzed for its physicochemical, structural and immunological characteristics and it was found to be antigenic, soluble, stable, non-allergenic and have a high affinity to its target receptor. Although the in silico analysis of the current study revealed that the designed multitope vaccine has the ability to trigger a specific immune response against M. catarrhalis, additional translational research is required to confirm the effectiveness of the designed vaccine.

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