scholarly journals Comprehensive and Quantitative Proteomic Analysis of Metamorphosis-Related Proteins in the Veined Rapa Whelk, Rapana venosa

2016 ◽  
Vol 17 (6) ◽  
pp. 924 ◽  
Author(s):  
Hao Song ◽  
Hai-Yan Wang ◽  
Tao Zhang
2010 ◽  
Vol 29 (1) ◽  
pp. 174-184 ◽  
Author(s):  
Ying-Fu Liu ◽  
Yong-Heng Chen ◽  
Mao-Yu Li ◽  
Peng-Fei Zhang ◽  
Fang Peng ◽  
...  

2020 ◽  
Vol 21 (2) ◽  
pp. 571 ◽  
Author(s):  
Jiayu Qi ◽  
Xiaoyun Zhao ◽  
Zhen Li

Jasmonates (JAs) regulate the defense of biotic and abiotic stresses, growth, development, and many other important biological processes in plants. The comprehensive proteomic profiling of plants under JAs treatment provides insights into the regulation mechanism of JAs. Isobaric tags for relative and absolute quantification (iTRAQ)-based quantitative proteomic analysis was performed on the Arabidopsis wild type (Ws) and JA synthesis deficiency mutant opr3-1. The effects of exogenous MeJA treatment on the proteome of opr3-1, which lacks endogenous JAs, were investigated. A total of 3683 proteins were identified and 126 proteins were differentially regulated between different genotypes and treatment groups. The functional classification of these differentially regulated proteins showed that they were involved in metabolic processes, responses to abiotic stress or biotic stress, the defense against pathogens and wounds, photosynthesis, protein synthesis, and developmental processes. Exogenous MeJA treatment induced the up-regulation of a large number of defense-related proteins and photosynthesis-related proteins, it also induced the down-regulation of many ribosomal proteins in opr3-1. These results were further verified by a quantitative real-time PCR (qRT-PCR) analysis of 15 selected genes. Our research provides the basis for further understanding the molecular mechanism of JAs’ regulation of plant defense, photosynthesis, protein synthesis, and development.


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