scholarly journals A High Redox Potential Laccase from Pycnoporus sanguineus RP15: Potential Application for Dye Decolorization

2016 ◽  
Vol 17 (5) ◽  
pp. 672 ◽  
Author(s):  
Ana Zimbardi ◽  
Priscila Camargo ◽  
Sibeli Carli ◽  
Sidney Aquino Neto ◽  
Luana Meleiro ◽  
...  
Keyword(s):  
Redox Potential ◽  
Potential Application ◽  
Dye Decolorization ◽  
Pycnoporus Sanguineus ◽  
High Redox Potential

scholarly journals Purification and Partial Characterization of a Thermostable Laccase from Pycnoporus sanguineus CS-2 with Ability to Oxidize High Redox Potential Substrates and Recalcitrant Dyes

10.5772/56374 ◽  
2013 ◽  
Author(s):  
Sergio M. Salcedo Martnez ◽  
Guadalupe Gutirrez-Soto ◽  
Carlos F. Rodriguez Garza ◽  
Tania J. Villarreal Galvan ◽  
Juan F. Contreras Cordero ◽  
...  
Keyword(s):  
Redox Potential ◽  
Partial Characterization ◽  
Pycnoporus Sanguineus ◽  
High Redox Potential

A few key residues determine the high redox potential shift in azurin mutants

2015 ◽  
Vol 13 (45) ◽  
pp. 11003-11013 ◽  
Author(s):  
Laura Zanetti-Polzi ◽  
Carlo A. Bortolotti ◽  
Isabella Daidone ◽  
Massimiliano Aschi ◽  
Andrea Amadei ◽  
...  
Keyword(s):  
Redox Potential ◽  
Potential Shift ◽  
Key Residues ◽  
High Redox Potential

The changes in the redox potential of Azurin upon mutation stem from the effects of a few key residues, including non-mutated ones, rather than being the result of a generalized rearrangement.

scholarly journals Titanium-based potassium-ion battery positive electrode with extraordinarily high redox potential

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Stanislav S. Fedotov ◽  
Nikita D. Luchinin ◽  
Dmitry A. Aksyonov ◽  
Anatoly V. Morozov ◽  
Sergey V. Ryazantsev ◽  
...  
Keyword(s):  
Redox Potential ◽  
Potassium Ion ◽  
Positive Electrode ◽  
High Redox Potential

The effect of pH and complexation on redox reactions between RS• radicals and flavins

1984 ◽  
Vol 62 (1) ◽  
pp. 171-177 ◽  
Author(s):  
Rizwan Ahmad ◽  
David A. Armstrong
Keyword(s):  
Redox Potential ◽  
Linear Function ◽  
Redox Reactions ◽  
Flavin Adenine Dinucleotide ◽  
Protonated Form ◽  
Redox Properties ◽  
The Other ◽  
Effect Of Ph ◽  
Other Hand ◽  
High Redox Potential

Elementary considerations indicate that thiol radicals, RS•, should have a high redox potential [Formula: see text][Formula: see text]However, the equilibrium [4],[Formula: see text]which is established in the presence of excess RS−, would convert RS•to [Formula: see text] which is a reducing species. Experimentally it was demonstrated that thiol radicals made by γ radiolysis of β-mercaptoethanol solutions effected two-electron oxidation of dihydroflavin FlH2 at pH 6.3 and of FlH− at pH 8. On the other hand, [Formula: see text] readily reduced Fl to FlH2 or FlH− as expected. At pH 9, photostationary states were established after a few minutes radiolysis and the ratios [FlH−]ss/[Fl]ss were a function of [Formula: see text] The main reactions occurring were:[Formula: see text]The values of k19 and k22 were both large. The ratio k19/k22 was ∼0.8 for lumiflavin and ∼0.3 for flavin adenine dinucleotide. The cyclic disulphide anions of lipoamide and dithiothreitol [Formula: see text] also effected two-electron reductions of flavins. However, the protonated form of [Formula: see text] oxidized FlH2, and the photostationary ratio [FlH−]ss/[Fl]ss was an approximate linear function of [Formula: see text]. The implications of the observed changes in redox properties of sulphur radicals on complexation with RS− and protonation were briefly considered.Des considérations élémentaires indiquent que les radicaux thiyles, RS•, doivent avoir un potentiel rédox élevé [Formula: see text][Formula: see text]

scholarly journals Enhanced Catalytic Dye Decolorization by Microencapsulation of Laccase from P. Sanguineus CS43 in Natural and Synthetic Polymers

Polymers ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1353
Author(s):  
Natalia Lopez-Barbosa ◽  
Ana Lucía Campaña ◽  
Juan C. Cruz ◽  
Nancy Ornelas-Soto ◽  
Johann F. Osma
Keyword(s):  
Catalytic Activity ◽  
Confocal Microscopy ◽  
Fluorescent Dyes ◽  
Fold Increase ◽  
Dye Decolorization ◽  
Reaction Rates ◽  
Lower Amount ◽  
Poly Vinyl Alcohol ◽  
Initial Reaction ◽  
Pycnoporus Sanguineus

Polymeric microcapsules with the fungal laccase from Pycnoporus sanguineus CS43 may represent an attractive avenue for the removal or degradation of dyes from wastewaters. Microcapsules of alginate/chitosan (9.23 ± 0.12 µm) and poly(styrenesulfonate) (PSS) (9.25 ± 0.35 µm) were synthesized and subsequently tested for catalytic activity in the decolorization of the diazo dye Congo Red. Successful encapsulation into the materials was verified via confocal microscopy of labeled enzyme molecules. Laccase activity was measured as a function of time and the initial reaction rates were recovered for each preparation, showing up to sevenfold increase with respect to free laccase. The ability of substrates to diffuse through the pores of the microcapsules was evaluated with the aid of fluorescent dyes and confocal microscopy. pH and thermal stability were also measured for encapsulates, showing catalytic activity for pH values as low as 4 and temperatures of about 80 °C. Scanning electron microscope (SEM) analyses demonstrated the ability of PSS capsules to avoid accumulation of byproducts and, therefore, superior catalytic performance. This was corroborated by the direct observation of substrates diffusing in and out of the materials. Compared with our PSS preparation, alginate/chitosan microcapsules studied by others degrade 2.6 times more dye, albeit with a 135-fold increase in units of enzyme per mg of dye. Similarly, poly(vinyl) alcohol microcapsules from degrade 1.7 times more dye, despite an eightfold increase in units of enzyme per mg of dye. This could be potentially beneficial from the economic viewpoint as a significantly lower amount of enzyme might be needed for the same decolorization level achieved with similar encapsulated systems.

Comparative analysis of gene sequences of three high-redox-potential laccases from basidiomycetes

2007 ◽  
Vol 417 (1) ◽  
pp. 348-351 ◽  
Author(s):  
E. A. Cherkashin ◽  
E. V. Stepanova ◽  
E. O. Landesman ◽  
O. V. Koroleva ◽  
V. I. Tishkov
Keyword(s):  
Comparative Analysis ◽  
Redox Potential ◽  
Gene Sequences ◽  
High Redox Potential
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