scholarly journals How to Study Biofilms after Microbial Colonization of Materials Used in Orthopaedic Implants

2016 ◽  
Vol 17 (3) ◽  
pp. 293 ◽  
Author(s):  
Lorenzo Drago ◽  
Serse Agrappi ◽  
Monica Bortolin ◽  
Marco Toscano ◽  
Carlo Romanò ◽  
...  
Keyword(s):  
Microbial Colonization ◽  
Orthopaedic Implants ◽  
Materials Used

Microbial colonization of different support materials used to enhance the methanogenic process

1994 ◽  
Vol 41 (4) ◽  
pp. 480-486
Author(s):  
J. M. Sanchez ◽  
S. Arijo ◽  
M. A. Mu�oz ◽  
M. A. Mori�igo ◽  
J. J. Borrego
Keyword(s):  
Microbial Colonization ◽  
Support Materials ◽  
Materials Used ◽  
Methanogenic Process

Microbial colonization of different support materials used to enhance the methanogenic process

1994 ◽  
Vol 41 (4) ◽  
pp. 480-486 ◽  
Author(s):  
J. M. Sanchez ◽  
S. Arijo ◽  
M. A. Mu�oz ◽  
M. A. Mori�igo ◽  
J. J. Borrego
Keyword(s):  
Microbial Colonization ◽  
Support Materials ◽  
Materials Used ◽  
Methanogenic Process

Wear analysis of materials used as orthopaedic implants

Wear ◽  
2006 ◽  
Vol 261 (9) ◽  
pp. 971-979 ◽  
Author(s):  
Jean Geringer ◽  
Bernard Forest ◽  
Pierre Combrade
Keyword(s):  
Orthopaedic Implants ◽  
Wear Analysis ◽  
Materials Used

scholarly journals Fretting-corrosion of materials used as orthopaedic implants

Wear ◽  
2005 ◽  
Vol 259 (7-12) ◽  
pp. 943-951 ◽  
Author(s):  
Jean Geringer ◽  
Bernard Forest ◽  
Pierre Combrade
Keyword(s):  
Fretting Corrosion ◽  
Orthopaedic Implants ◽  
Materials Used

Microbial colonization of different support materials used to enhance the methanogenic process

1994 ◽  
Vol 41 (4) ◽  
pp. 480-486 ◽  
Author(s):  
J. M. Sanchez ◽  
S. Arijo ◽  
M. A. Mu�oz ◽  
M. A. Mori�igo ◽  
J. J. Borrego
Keyword(s):  
Microbial Colonization ◽  
Support Materials ◽  
Materials Used ◽  
Methanogenic Process

scholarly journals Microbial colonization and persistence in deep fractured shales is guided by metabolic exchanges and viral predation

Microbiome ◽  
2022 ◽  
Vol 10 (1) ◽  
Author(s):  
Kaela K. Amundson ◽  
Mikayla A. Borton ◽  
Rebecca A. Daly ◽  
David W. Hoyt ◽  
Allison Wong ◽  
...  
Keyword(s):  
Microbial Community ◽  
Hydraulic Fracturing ◽  
Microbial Colonization ◽  
Organic Substrates ◽  
Community Interactions ◽  
Deep Subsurface ◽  
Shale Formations ◽  
Temporal Sampling ◽  
Fracturing Process ◽  
Materials Used

Abstract Background Microbial colonization of subsurface shales following hydraulic fracturing offers the opportunity to study coupled biotic and abiotic factors that impact microbial persistence in engineered deep subsurface ecosystems. Shale formations underly much of the continental USA and display geographically distinct gradients in temperature and salinity. Complementing studies performed in eastern USA shales that contain brine-like fluids, here we coupled metagenomic and metabolomic approaches to develop the first genome-level insights into ecosystem colonization and microbial community interactions in a lower-salinity, but high-temperature western USA shale formation. Results We collected materials used during the hydraulic fracturing process (i.e., chemicals, drill muds) paired with temporal sampling of water produced from three different hydraulically fractured wells in the STACK (Sooner Trend Anadarko Basin, Canadian and Kingfisher) shale play in OK, USA. Relative to other shale formations, our metagenomic and metabolomic analyses revealed an expanded taxonomic and metabolic diversity of microorganisms that colonize and persist in fractured shales. Importantly, temporal sampling across all three hydraulic fracturing wells traced the degradation of complex polymers from the hydraulic fracturing process to the production and consumption of organic acids that support sulfate- and thiosulfate-reducing bacteria. Furthermore, we identified 5587 viral genomes and linked many of these to the dominant, colonizing microorganisms, demonstrating the key role that viral predation plays in community dynamics within this closed, engineered system. Lastly, top-side audit sampling of different source materials enabled genome-resolved source tracking, revealing the likely sources of many key colonizing and persisting taxa in these ecosystems. Conclusions These findings highlight the importance of resource utilization and resistance to viral predation as key traits that enable specific microbial taxa to persist across fractured shale ecosystems. We also demonstrate the importance of materials used in the hydraulic fracturing process as both a source of persisting shale microorganisms and organic substrates that likely aid in sustaining the microbial community. Moreover, we showed that different physicochemical conditions (i.e., salinity, temperature) can influence the composition and functional potential of persisting microbial communities in shale ecosystems. Together, these results expand our knowledge of microbial life in deep subsurface shales and have important ramifications for management and treatment of microbial biomass in hydraulically fractured wells.

scholarly journals Feasibility of Using H3PO4/H2O2 in the Synthesis of Antimicrobial TiO2 Nanoporous Surfaces

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Benjamín Valdez-Salas ◽  
Ernesto Beltrán-Partida
Keyword(s):  
Antimicrobial Activity ◽  
Microbial Colonization ◽  
Control Material ◽  
Industrial Transfer ◽  
Stable Temperature ◽  
Mixture Preparation ◽  
Materials Used ◽  
Primary Materials ◽  
Nanoporous Surfaces ◽  
Cellular Lysis

Ti6Al4V alloys are the primary materials used for clinical bone regeneration and restoration; however, they are substantially susceptible to biomaterial-related infections. Therefore, in the present work, we applied a controllable and stable oxidative nanopatterning strategy by applying H3PO4, a weaker dissociating acid, as a substitute for H2SO4 in the classical piranha reaction. The results suggest that our method acted as a concomitant platform to develop reproducible diameter-controlled TiO2 nanopores (NPs). Interestingly, our procedure illustrated stable temperature reactions without exothermic responses since the addition of mixture preparation to the nanopatterning reactions. The reactions were carried out for 30 min (NP14), 1 h (NP7), and 2 h (NP36), suggesting the formation of a thin nanopore layer as observed by Raman spectroscopy. Moreover, the antimicrobial activity revealed that NP7 could disrupt active microbial colonization for 2 h and 6 h. The phenotype configuration strikingly showed that NP7 does not alter the cell morphology, thus proposing a disruptive adhesion pathway instead of cellular lysis. Furthermore, preliminary assays suggested an early promoted osteoblasts viability in comparison to the control material. Our work opens a new path for the rationale design of nanobiomaterials with “intelligent surfaces” capable of decreasing microbial adhesion, increasing osteoblast viability, and being scalable for industrial transfer.

Deformation Replication

1970 ◽  
Vol 28 ◽  
pp. 280-281
Author(s):  
J. Temple Black
Keyword(s):  
Cutting Speed ◽  
Machining Process ◽  
Depth Of Cut ◽  
Rake Face ◽  
Orthogonal Machining ◽  
Aluminum Chips ◽  
Before And After ◽  
Materials Used ◽  
Glass Knife ◽  
Very High

Tool materials used in ultramicrotomy are glass, developed by Latta and Hartmann (1) and diamond, introduced by Fernandez-Moran (2). While diamonds produce more good sections per knife edge than glass, they are expensive; require careful mounting and handling; and are time consuming to clean before and after usage, purchase from vendors (3-6 months waiting time), and regrind. Glass offers an easily accessible, inexpensive material ($0.04 per knife) with very high compressive strength (3) that can be employed in microtomy of metals (4) as well as biological materials. When the orthogonal machining process is being studied, glass offers additional advantages. Sections of metal or plastic can be dried down on the rake face, coated with Au-Pd, and examined directly in the SEM with no additional handling (5). Figure 1 shows aluminum chips microtomed with a 75° glass knife at a cutting speed of 1 mm/sec with a depth of cut of 1000 Å lying on the rake face of the knife.

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