scholarly journals Deep Sequencing and Screening of Differentially Expressed MicroRNAs Related to Milk Fat Metabolism in Bovine Primary Mammary Epithelial Cells

2016 ◽  
Vol 17 (2) ◽  
pp. 200 ◽  
Author(s):  
Binglei Shen ◽  
Liying Zhang ◽  
Chuanjiang Lian ◽  
Chunyan Lu ◽  
Yonghong Zhang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Deep Sequencing ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Fat Metabolism ◽  
Mammary Epithelial ◽  
Differentially Expressed

scholarly journals The effect of short/branched chain acyl-coenzyme A dehydrogenase gene on triglyceride synthesis of bovine mammary epithelial cells

2018 ◽  
Vol 61 (1) ◽  
pp. 115-122 ◽  
Author(s):  
Ping Jiang ◽  
Xibi Fang ◽  
Zhihui Zhao ◽  
Xianzhong Yu ◽  
Boxing Sun ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Dairy Cattle ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Fat Metabolism ◽  
Mammary Epithelial ◽  
Differentially Expressed ◽  
Bovine Mammary Epithelial Cells ◽  
Chain Acyl ◽  
Branched Chain

Abstract. Short/branched chain acyl-CoA dehydrogenase (ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids. Our previous transcriptome analysis in dairy cattle showed that ACADSB was differentially expressed and was associated with milk fat metabolism. The aim of this study was to elucidate the background of this differential expression and to evaluate the role of ACADSB as a candidate for fat metabolism in dairy cattle. After analysis of ACADSB mRNA abundance by qRT-PCR and Western blot, overexpression and RNA interference (RNAi) vectors of ACADSB gene were constructed and then transfected into bovine mammary epithelial cells (bMECs) to examine the effects of ACADSB on milk fat synthesis. The results showed that the ACADSB was differentially expressed in mammary tissue of low and high milk fat dairy cattle. Overexpression of ACADSB gene could significantly increase the level of intracellular triglyceride (TG), while ACADSB gene knockdown could significantly reduce the TG synthesis in bMECs. This study suggested that the ACADSB was important in TG synthesis in bMECs, and it could be a candidate gene to regulate the metabolism of milk fat in dairy cattle.

ADIPOR1 regulates genes involved in milk fat metabolism in goat mammary epithelial cells

2021 ◽  
Author(s):  
Wangsheng Zhao ◽  
Michael Adjei ◽  
Hongmei Wang ◽  
Yueling Yangliu ◽  
Jiangjiang Zhu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Fat Metabolism ◽  
Mammary Epithelial ◽  
Goat Mammary Epithelial Cells

MicroRNA-432 inhibits milk fat synthesis by targeting SCD and LPL in ovine mammary epithelial cells

2021 ◽  
Author(s):  
Zhiyun Hao ◽  
Yuzhu Luo ◽  
Jiqing Wang ◽  
Jon Hickford ◽  
Huitong Zhou ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Epithelial Cells ◽  
Milk Production ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Differentially Expressed ◽  
Differentially Expressed Mirnas ◽  
Fat Synthesis ◽  
Milk Fat Synthesis

In our previous studies, microRNA-432 (miR-432) was found to be one of differentially expressed miRNAs in ovine mammary gland between the two breeds of lactating sheep with different milk production...

MiR-183 regulates milk fat metabolism via MST1 in goat mammary epithelial cells

Gene ◽  
2018 ◽  
Vol 646 ◽  
pp. 12-19 ◽  
Author(s):  
Zhi Chen ◽  
HuaiPing Shi ◽  
Shuang Sun ◽  
Jun Luo ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Fat Metabolism ◽  
Mammary Epithelial ◽  
Goat Mammary Epithelial Cells

scholarly journals PRL/microRNA-183/IRS1 Pathway Regulates Milk Fat Metabolism in Cow Mammary Epithelial Cells

Genes ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 196 ◽  
Author(s):  
Peixin Jiao ◽  
Yuan Yuan ◽  
Meimei Zhang ◽  
Youran Sun ◽  
Chuanzi Wei ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Epithelial Cells ◽  
Dairy Cows ◽  
Molecular Mechanism ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Cpg Island ◽  
Fat Metabolism ◽  
Milk Quality ◽  
Mammary Epithelial

The aim of the study was to understand the internal relationship between milk quality and lipid metabolism in cow mammary glands. A serial of studies was conducted to assess the molecular mechanism of PRL/microRNA-183/IRS1 (Insulin receptor substrate) pathway, which regulates milk fat metabolism in dairy cows. microRNA-183 (miR-183) was overexpressed and inhibited in cow mammary epithelial cells (CMECs), and its function was detected. The function of miR-183 in inhibiting milk fat metabolism was clarified by triglycerides (TAG), cholesterol and marker genes. There is a CpG island in the 5′-flanking promoter area of miR-183, which may inhibit the expression of miR-183 after methylation. Our results showed that prolactin (PRL) inhibited the expression of miR-183 by methylating the 5′ terminal CpG island of miR-183. The upstream regulation of PRL on miR-183 was demonstrated, and construction of the lipid metabolism regulation network of microRNA-183 and target gene IRS1 was performed. These results reveal the molecular mechanism of PRL/miR-183/IRS1 pathway regulating milk fat metabolism in dairy cows, thus providing an experimental basis for the improvement of milk quality.

MicroRNA-mRNA regulatory network related to lipid metabolism in bovine mammary epithelial cells

2020 ◽  
Author(s):  
Lixin Xia ◽  
Zhihui Zhao ◽  
Chunyan Lu ◽  
Ping Jiang ◽  
Haibin Yu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Conjoint Analysis ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Differentially Expressed ◽  
Luciferase Reporter ◽  
Fat Percentage ◽  
Triglyceride Synthesis ◽  
Bovine Mammary Epithelial Cells

Abstract Milk fat percentage is an important factor of milk quality in dairy cattle. Functional microRNAs and genes can affect lipid synthesis and metabolism through differential expression in bovine mammary epithelial cells (BMECs). It is necessary to screening the crucial candidate gene and miRNA on milk fat percentage. In this study, we extract total RNA of BMECs isolated from Chinese Holstein cows with high and low milk fat percentages for the conjoint analysis of RNA-seq and Solexa sequencing data. 190 differentially expressed genes and 33 differentially expressed microRNAs (DERs) were enriched in 488 GO terms and 12 KEGG pathways significantly (p <0.05) based on the conjoint analysis. The detection of triglyceride production in BMECs showed that bta-miR-21-3p and bta-miR-148a promote triglyceride synthesis, whereas bta-miR-124a, bta-miR-877, bta-miR-2382-5p and bta-miR-2425-5p inhibit triglyceride synthesis. Meanwhile, the target relationships between PDE4D and bta-miR-148a, PEG10 and bta-miR-877, SOD3 and bta-miR-2382-5p, and ADAMTS1 and bta-miR-2425-5p were verified using luciferase reporter assays and quantitative RT-PCR. The conjoint analysis can more accurately screen candidate regulator related to milk fat percentage at the molecular level, which provided a scientific research method screening functional miRNA and gene for the breeding of new high-quality dairy cows.

Liver X receptor α participates in LPS-induced reduction of triglyceride synthesis in bovine mammary epithelial cells

2020 ◽  
pp. 1-7
Author(s):  
Jianfa Wang ◽  
Shuai Lian ◽  
Jun Song ◽  
Hai Wang ◽  
Xu Zhang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Epithelial Cells ◽  
Dairy Cow ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Liver X Receptor ◽  
Mammary Epithelial ◽  
Triglyceride Synthesis ◽  
Milk Fat Depression ◽  
Liver X Receptor Α

Abstract Lipopolysaccharides (LPS) could induce milk fat depression via regulating the body and blood fat metabolism. However, it is not completely clear how LPS might regulate triglyceride synthesis in dairy cow mammary epithelial cells (DCMECs). DCMECs were isolated and purified from dairy cow mammary tissue and treated with LPS. The level of triglyceride synthesis, the expression and activity of the liver X receptor α (LXRα), enzymes related to de novo fatty acid synthesis, and the expression of the fatty acid transporters were investigated. We found that LPS decreased the level of triglyceride synthesis via a down-regulation of the transcription, translation, and nuclear translocation level of the LXRα. The results also indicated that the transcription level of the LXRα target genes, sterol regulatory element binding protein 1 (SREBP1), fatty acid synthetase (FAS), acetyl-CoA carboxylase-1 (ACC1), were significantly down-regulated in DCMECs after LPS treatment. Our data may provide new insight into the mechanisms of milk fat depression caused by LPS.

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