scholarly journals A Co-Culture Model of Fibroblasts and Adipose Tissue-Derived Stem Cells Reveals New Insights into Impaired Wound Healing After Radiotherapy

2015 ◽  
Vol 16 (11) ◽  
pp. 25947-25958 ◽  
Author(s):  
Frank Haubner ◽  
Dominique Muschter ◽  
Fabian Pohl ◽  
Stephan Schreml ◽  
Lukas Prantl ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Adipose Tissue ◽  
Impaired Wound Healing ◽  
Culture Model

scholarly journals Adipose Tissue: A Source of Stem Cells with Potential for Regenerative Therapies for Wound Healing

2020 ◽  
Vol 9 (7) ◽  
pp. 2161 ◽  
Author(s):  
Lucy V Trevor ◽  
Kirsten Riches-Suman ◽  
Ajay L Mahajan ◽  
M Julie Thornton
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Adipose Tissue ◽  
Cell Types ◽  
Anatomical Location ◽  
Impaired Wound Healing ◽  
Cell Lineages ◽  
Different Types ◽  
No Gold Standard ◽  
Regenerative Therapies

Interest in adipose tissue is fast becoming a focus of research after many years of being considered as a simple connective tissue. It is becoming increasingly apparent that adipose tissue contains a number of diverse cell types, including adipose-derived stem cells (ASCs) with the potential to differentiate into a number of cell lineages, and thus has significant potential for developing therapies for regenerative medicine. Currently, there is no gold standard treatment for scars and impaired wound healing continues to be a challenge faced by clinicians worldwide. This review describes the current understanding of the origin, different types, anatomical location, and genetics of adipose tissue before discussing the properties of ASCs and their promising applications for tissue engineering, scarring, and wound healing.

Comparison of the Effect of Adipocyte-derived Stem Cells and Curcumin Nanoliposomes with Phenytoin on Open Cutaneous Wound Healing in Rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Mohammadreza Ebrahimzade ◽  
Mohammad Mirdoraghi ◽  
Ameneh Alikarami ◽  
Sahar Heidari ◽  
Tayebeh Rastegar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Adipose Tissue ◽  
Healing Time ◽  
Daily Work ◽  
Expression Of Genes ◽  
Skin Ulcers ◽  
Immobility Time ◽  
Liposomal Nanoparticles ◽  
Chromosome Staining

Background: Reducing the healing time of wounds can decrease the patient`s immobility time and their medical costs,leading a faster return of the patients to daily work. Objective: To compare the effect of adipose-derived stem cells and curcumin-containing liposomal nanoparticles with phenytoin on wound healing. Method: After anesthesia of the rats, open skin ulcers were made by a bistoury blade.Subsequently,stem cells were re-moved from the adipose tissue of theupper border of the epididymis. Then,the originality of stem cells was confirmed by the flow cytometry. The fusion method was used to prepare the liposome;and also nanoliposomal particles wereconfirmedby using the DLS microscope.The percentage of recovery and the cell count was measured with IMAGEJ.The expression of genes was assessed by PCR. The number of fibro blasts was counted by immuno histo chemistry techniques.The amount of collagen was determined by Tri-chromosome staining and the number of capillaries was enumerated byH & E staining. Results: The expression of TGF-β1 gene, vascular number, wound healing rate and the numberof fibroblasts increased significantly in adipose tissue-derived stem cells and curcumin nanoliposome groups(p<0.05);the wound surface was also decreased significantly(p<0.05). Conclusion: Based on the results of our research, adipose tissue-derived stem cells and curcumin nanoliposomescan heal wounds efficiently.

Microvesicles enhance the mobility of human diabetic adipose tissue-derived mesenchymal stem cells in vitro and improve wound healing in vivo

2016 ◽  
Vol 473 (4) ◽  
pp. 1111-1118 ◽  
Author(s):  
Nhu Thuy Trinh ◽  
Toshiharu Yamashita ◽  
Tran Cam Tu ◽  
Toshiki Kato ◽  
Kinuko Ohneda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Improve Wound Healing

220 Exploring the use of mesenchymal stem cells for treatment of mastitis and metritis in cattle

2020 ◽  
Vol 32 (2) ◽  
pp. 238
Author(s):  
R. Singh ◽  
S. Saini ◽  
S. Ansari ◽  
S. Jamwal ◽  
D. Malakar
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Real Time ◽  
Real Time Pcr ◽  
Local Group ◽  
Control Group ◽  
Time Intervals ◽  
And Control

The present study was carried out to isolate mesenchymal stem cells (MSCs) from adipose tissue of cattle (Bos indicus), characterise them, and apply them for the treatment of mastitis and metritis in the cow. Cattle MSCs were isolated from adipose tissue near the loin region of cow. Isolated adipose tissue was subjected to enzymatic digestion using 2% collagenase with agitation at regular intervals. The cells obtained after digestion were resuspended in cell culture flasks containing growth enriched medium and cultured under standard culture conditions. Alkaline phosphatase staining was used as one of the parameters to confirm cultured putative MSCs. Bovine Ad-MSCs were further characterised using real time-PCR by amplification of MSC-specific markers: CD73, CD90, and CD105 as positive markers and CD34, CD45, and CD79a as negative markers. Immunocytochemistry showed the presence of CD73, CD90, and CD105 on the cell surface. Three groups-control (C), local (L), and intravenous (IV)-with 6 cows suffering from mastitis were taken in each group and subjected to MSC transplantation through local and intravenous routes. Control group animals were subjected to antibiotic treatment only. Similarly, another three groups were taken with 6 cows in each group suffering from metritis. Post-transplantation wound healing, tissue repair, and reduction in inflammation were monitored for 26 days, at different time intervals; that is, after Days 1, 3, 7, and 15. Blood samples were also collected from animals at the same time intervals for real time-PCR. A similar examination was also done in metritis groups along with the analysis of the reduction in turbidity of cervical fluid at the abovementioned time intervals. Real time-PCR was performed to determine relative expression of genes for proliferative factors, anti-inflammatory cytokines, and antimicrobial peptides on cells isolated from blood collected at different time intervals. Gene expression in the local group of mastitis subjected to MSC injection was significantly higher than that of the IV and control group. The somatic cell count declined in both local and IV groups compared with the control group. Whereas the expression of the same genes in the IV group of metritis was significantly higher than that of the local and control groups of cows. The turbidity of cervical fluid and mucus was reduced in the IV group compared with the local group. In conclusion, we demonstrated the healing potential of MSCs in a cow model via MSC injection. Promising results were obtained in curing mastitis in both local and IV groups, whereas healing in the case of metritis was significantly higher in the IV group compared with both the control and local groups of cows. The study indicates the potential use of MSc for treatment of mastitis and metritis in cattle through wound healing and decreasing microbial infection.

scholarly journals Stem cells from adipose tissue improve the time of wound healing in rats

2016 ◽  
Vol 31 (12) ◽  
pp. 821-825 ◽  
Author(s):  
Camila Melo Ohashi ◽  
◽  
Fabio Alves Morikawa Caldeira ◽  
Denilson José Silva Feitosa-Junior ◽  
André Lopes Valente ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Adipose Tissue

Amniotic Membrane Loading Epidermal Stem Cells Accelerate Impaired Wound Healing in Diabetic Rats

2011 ◽  
Vol 214 ◽  
pp. 455-460
Author(s):  
Qing Ling Zhong ◽  
De Wu Liu ◽  
Fan Rong Liu
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Amniotic Membrane ◽  
Healing Rate ◽  
Diabetic Rats ◽  
Epidermal Stem Cells ◽  
Impaired Wound Healing ◽  
Wound Model ◽  
Wound Margin

Epidermal stem cells are essential in wound healing, but their amount and activity are decreased in diabetes which contributes to the impaired healing. This study evaluated the efficacy of amniotic membrane loading epidermal stem cells in the management of impaired wound in diabetes. Epidermal stem cells derived from SD rats were isolated, cultured, identified and labled with BrdU in vitro. The wound model of diabetic rats were established, then amniotic membrane loading labled BrdU epidermal stem cells were implanted to impaired wound. The results showed that wound healing rate in amniotic membrane loading epidermal stem cells was significantly higher than that in control groups 14 days after treatment. BrdU-positive cells in the wounds and newborn epidermis were visible. This indicated that amniotic membrane loading epidermal stem cells accelerates epidermal migration of wound margin and wound epithelialization in diabetic rats.

Sign in / Sign up

Export Citation Format

Share Document