scholarly journals A Comparative Study of Non-Viral Gene Delivery Techniques to Human Adipose-Derived Mesenchymal Stem Cell

2014 ◽  
Vol 15 (9) ◽  
pp. 15044-15060 ◽  
Author(s):  
Nur Halim ◽  
Kamal Fakiruddin ◽  
Syed Ali ◽  
Badrul Yahaya
Keyword(s):  
Stem Cell ◽  
Gene Delivery ◽  
Mesenchymal Stem Cell ◽  
Comparative Study ◽  
Viral Gene ◽  
Viral Gene Delivery ◽  
Delivery Techniques

scholarly journals Dual non-viral gene delivery from microparticles within 3D high-density stem cell constructs for enhanced bone tissue engineering

Biomaterials ◽  
2018 ◽  
Vol 161 ◽  
pp. 240-255 ◽  
Author(s):  
Alexandra McMillan ◽  
Minh Khanh Nguyen ◽  
Tomas Gonzalez-Fernandez ◽  
Peilin Ge ◽  
Xiaohua Yu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Gene Delivery ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
High Density ◽  
Viral Gene ◽  
Viral Gene Delivery

Viral and non-viral gene delivery and its role in pluripotent stem cell engineering

2008 ◽  
Vol 5 (4) ◽  
pp. e107-e115 ◽  
Author(s):  
Nicole M. Kane ◽  
Scott McRae ◽  
Chris Denning ◽  
Andrew H. Baker
Keyword(s):  
Stem Cell ◽  
Gene Delivery ◽  
Pluripotent Stem Cell ◽  
Cell Engineering ◽  
Viral Gene ◽  
Stem Cell Engineering ◽  
Viral Gene Delivery

Innovative Collagen Nano-Hydroxyapatite Scaffolds Offer a Highly Efficient Non-Viral Gene Delivery Platform for Stem Cell-Mediated Bone Formation

2012 ◽  
Vol 24 (6) ◽  
pp. 749-754 ◽  
Author(s):  
Caroline M. Curtin ◽  
Gráinne M. Cunniffe ◽  
Frank G. Lyons ◽  
Kazuhisa Bessho ◽  
Glenn R. Dickson ◽  
...  
Keyword(s):  
Stem Cell ◽  
Gene Delivery ◽  
Bone Formation ◽  
Viral Gene ◽  
Highly Efficient ◽  
Delivery Platform ◽  
Viral Gene Delivery

Recent advances in the analysis full/empty capsid ratio and genome integrity of adeno-associated virus (AAV) gene delivery vectors

2020 ◽  
Vol 20 ◽  
Author(s):  
L. Hajba ◽  
A. Guttman
Keyword(s):  
Gene Delivery ◽  
High Efficiency ◽  
Analytical Techniques ◽  
Viral Gene ◽  
Adeno Associated Virus ◽  
Gene Delivery Vectors ◽  
Empty Capsid ◽  
Purification Methods ◽  
Viral Gene Delivery

: Adeno-associated virus (AAV) is one of the most promising viral gene delivery vectors with long-term gene expression and disease correction featuring high efficiency and excellent safety in human clinical trials. During the production of AAV vectors,there are several quality control (QC)parameters that should be rigorously monitored to comply with clini-cal safety and efficacy. This review gives a short summary of the most frequently used AVV production and purification methods,focusing on the analytical techniques applied to determine the full/empty capsid ratio and the integrity of the encapsidated therapeutic DNA of the products.

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