scholarly journals DNA Sequencing Diagnosis of Off-Season Spirochetemia with Low Bacterial Density in Borrelia burgdorferi and Borrelia miyamotoi Infections

2014 ◽  
Vol 15 (7) ◽  
pp. 11364-11386 ◽  
Author(s):  
Sin Lee ◽  
Jessica Vigliotti ◽  
Veronica Vigliotti ◽  
William Jones ◽  
Thomas Moorcroft ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Dna Sequencing ◽  
Bacterial Density ◽  
Borrelia Miyamotoi

scholarly journals Surveillance of Ticks and Tick-Borne Pathogens in Suburban Natural Habitats of Central Maryland

2021 ◽  
Author(s):  
Matthew T Milholland ◽  
Lars Eisen ◽  
Robyn M Nadolny ◽  
Andrias Hojgaard ◽  
Erika T Machtinger ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Spotted Fever ◽  
Babesia Microti ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Spotted Fever Group ◽  
Borrelia Miyamotoi ◽  
Natural Habitats ◽  
Questing Ticks ◽  
Tick Vectors

Abstract Lyme and other tick-borne diseases are increasing in the eastern United States and there is a lack of research on integrated strategies to control tick vectors. Here we present results of a study on tick-borne pathogens detected from tick vectors and rodent reservoirs from an ongoing 5-yr tick suppression study in the Lyme disease-endemic state of Maryland, where human-biting tick species, including Ixodes scapularis Say (Acari: Ixodidae) (the primary vector of Lyme disease spirochetes), are abundant. During the 2017 tick season, we collected 207 questing ticks and 602 ticks recovered from 327 mice (Peromyscus spp. (Rodentia: Cricetidae)), together with blood and ear tissue from the mice, at seven suburban parks in Howard County. Ticks were selectively tested for the presence of the causative agents of Lyme disease (Borrelia burgdorferi sensu lato [s.l.]), anaplasmosis (Anaplasma phagocytophilum), babesiosis (Babesia microti), ehrlichiosis (Ehrlichia ewingii, Ehrlichia chaffeensis, and ‘Panola Mountain’ Ehrlichia) and spotted fever group rickettsiosis (Rickettsia spp.). Peromyscus ear tissue and blood samples were tested for Bo. burgdorferi sensu stricto (s.s), A. phagocytophilum, Ba. microti, and Borrelia miyamotoi. We found 13.6% (15/110) of questing I. scapularis nymphs to be Bo. burgdorferi s.l. positive and 1.8% (2/110) were A. phagocytophilum positive among all sites. Borrelia burgdorferi s.s. was found in 71.1% (54/76) of I. scapularis nymphs removed from mice and 58.8% (194/330) of captured mice. Results from study on tick abundance and pathogen infection status in questing ticks, rodent reservoirs, and ticks feeding on Peromyscus spp. will aid efficacy evaluation of the integrated tick management measures being implemented.

scholarly journals Detection of Borrelia burgdorferi Sensu Lato and Relapsing Fever Borrelia in Feeding Ixodes Ticks and Rodents in Sarawak, Malaysia: New Geographical Records of Borrelia yangtzensis and Borrelia miyamotoi

Pathogens ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 846
Author(s):  
Alice C. C. Lau ◽  
Yongjin Qiu ◽  
Mohamed Abdallah Mohamed Moustafa ◽  
Ryo Nakao ◽  
Michito Shimozuru ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Primary Forest ◽  
Borrelia Miyamotoi ◽  
Relapsing Fever ◽  
Ixodes Ticks ◽  
Ixodes Granulatus ◽  
New Geographical Record ◽  
Etiological Agents ◽  
Malaysian Borneo

Members of the Borrelia burgdorferi sensu lato (Bbsl) complex are etiological agents of Lyme disease (LD), and Borrelia miyamotoi is one of the relapsing fever Borrelia (RFB). Despite the serological evidence of LD in Malaysia, there has been no report from Sarawak, Malaysian Borneo. Thus, this study aimed to detect and characterize Borrelia in rodents and Ixodes ticks from primary forests and an oil palm (OP) plantation in Sarawak. Borrelia yangtzensis (a member of the Bbsl complex) was detected in 43.8% (14/32) of Ixodes granulatus; most of the positive ticks were from the OP plantation (13/14). Out of 56 rodents, B. yangtzensis was detected in four Rattus spp. from the OP plantation and B. miyamotoi was detected in one rodent, Sundamys muelleri, from the primary forest. Further, the positive samples of B. yangtzensis were randomly selected for multilocus sequence analysis (MLSA). The MLSA results of successfully amplified tick samples revealed a clustering with the sequences isolated from Japan and China. This study is the first evidence of B. miyamotoi, a known human pathogen in Malaysia, and B. yangtzensis, which is circulating in ticks and rodents in Sarawak, Malaysian Borneo, and presenting a new geographical record of the Borrelia spp.

scholarly journals Questing Ixodes ricinus ticks (Acari, Ixodidae) as a vector of Borrelia burgdorferi sensu lato and Borrelia miyamotoi in an urban area of north-eastern Poland

2019 ◽  
Vol 78 (1) ◽  
pp. 113-126 ◽  
Author(s):  
Katarzyna Kubiak ◽  
Janina Dziekońska-Rynko ◽  
Hanna Szymańska ◽  
Dariusz Kubiak ◽  
Małgorzata Dmitryjuk ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Urban Area ◽  
Ixodes Ricinus ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Borrelia Miyamotoi ◽  
North Eastern ◽  
Ixodes Ricinus Ticks

Neues zu zeckenübertragenen Infektionen

2017 ◽  
Vol 142 (11) ◽  
pp. 805-810
Author(s):  
Johannes Borde ◽  
Gerhard Dobler ◽  
Siegbert Rieg
Keyword(s):  
Borrelia Burgdorferi ◽  
Francisella Tularensis ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Borrelia Miyamotoi ◽  
Emerging Pathogen

Was ist neu? Frühsommer-Meningoenzephalitis (FSME) In Europa nimmt die Ausbreitung von FSME in Richtung Westen zu. Erstmalig ist 2016 ein autochthoner humaner FSME-Fall in den Niederlanden dokumentiert worden. Die FSME-Erkrankungsfälle im Landkreis Reutlingen rücken die alimentäre FSME-Transmission in Deutschland wieder in das Bewusstsein. Tularämie (Hasenpest) Francisella tularensis hat ein breites Wirtsspektrum, von Kleinsäugern bis hin zu Arthropoden, wie z. B. Schildzecken. Die Transmission durch Zeqcken wurde bisher wenig beachtet. Vor dem Hintergrund neuerer epidemiologischen Erkenntnisse sollte auch nach Zeckenstichen mit entsprechenden Symptomen eine Francisella tularensis Infektion in Erwägung gezogen werden. Persistierende Beschwerden bei Borreliose (Borrelia-burgdorferi-sensu-lato-Komplex) Eine aktuelle Studie untersuchte den Effekt einer verlängerten antiinfektiven Behandlungsdauer auf persistierende Symptome, die mit einer Borrelieninfektion (Borrelia-burgdorferi-sensu-lato-Komplex) in Verbindung gebracht werden können. Die Therapie über 2 Wochen führte zu einer quantitativ gleichen und ebenso nachhaltigen Zunahme der Lebensqualität wie ein verlängertes Schema. Borrelia miyamotoi ist ein „Emerging Pathogen“ – erstmalig konnte nun auch in Deutschland ein humaner Infektionsfall nachgewiesen werden.

scholarly journals Borrelia burgdorferi and Borrelia miyamotoi seroprevalence in California blood donors

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243950
Author(s):  
Sharon I. Brummitt ◽  
Anne M. Kjemtrup ◽  
Danielle J. Harvey ◽  
Jeannine M. Petersen ◽  
Christopher Sexton ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Western Blot ◽  
Sierra Nevada ◽  
Blood Donors ◽  
Test System ◽  
Emerging Diseases ◽  
Borrelia Miyamotoi ◽  
Increased Risk ◽  
Significant Difference

The western blacklegged tick, Ixodes pacificus, an important vector in the western United States of two zoonotic spirochetes: Borrelia burgdorferi (also called Borreliella burgdorferi), causing Lyme disease, and Borrelia miyamotoi, causing a relapsing fever-type illness. Human cases of Lyme disease are well-documented in California, with increased risk in the north coastal areas and western slopes of the Sierra Nevada range. Despite the established presence of B. miyamotoi in the human-biting I. pacificus tick in California, clinical cases with this spirochete have not been well studied. To assess exposure to B. burgdorferi and B. miyamotoi in California, and to address the hypothesis that B. miyamotoi exposure in humans is similar in geographic range to B. burgdorferi, 1,700 blood donor sera from California were tested for antibodies to both pathogens. Sampling was from high endemic and low endemic counties for Lyme disease in California. All sera were screened using the C6 ELISA. All C6 positive and equivocal samples and nine randomly chosen C6 negative samples were further analyzed for B. burgdorferi antibody using IgG western blot and a modified two ELISA test system and for B. miyamotoi antibody using the GlpQ ELISA and B. miyamotoi whole cell sonicate western blot. Of the 1,700 samples tested in series, eight tested positive for antibodies to B. burgdorferi (0.47%, Exact 95% CI: 0.20, 0.93) and two tested positive for antibodies to B. miyamotoi (0.12%, Exact 95% CI: 0.01, 0.42). There was no statistically significant difference in seroprevalence for either pathogen between high and low Lyme disease endemic counties. Our results confirm a low frequency of Lyme disease and an even lower frequency of B. miyamotoi exposure among adult blood donors in California; however, our findings reinforce public health messaging that there is risk of infection by these emerging diseases in the state.

scholarly journals Prevalence of pathogens in ticks collected from humans through citizen science in Belgium

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Tinne Lernout ◽  
Nick De Regge ◽  
Katrien Tersago ◽  
Manoj Fonville ◽  
Vanessa Suin ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Citizen Science ◽  
Lyme Borreliosis ◽  
Great Majority ◽  
Dermacentor Reticulatus ◽  
Human Infection ◽  
Borrelia Miyamotoi ◽  
Tick Borne Encephalitis ◽  
Tick Borne Encephalitis Virus ◽  
First Time

Abstract Background In order to evaluate the risk of human exposure to tick-borne pathogens in Belgium, a study on the prevalence of several pathogens was conducted on feeding ticks removed from humans in 2017. Methods Using a citizen science approach based on an existing notification tool for tick bites, a sample of ticks was collected across the country. Collected ticks were screened by PCR for the presence of the following pathogens: Anaplasma phagocytophilum, Babesia spp., Borrelia burgdorferi (sensu lato), Borrelia miyamotoi, Neoehrlichia mikurensis, Rickettsia helvetica and tick-borne encephalitis virus (TBEV). Results In total, 1599 ticks were included in the sample. The great majority of ticks belonged to Ixodes ricinus (99%); other tick species were identified as Ixodes hexagonus (0.7%) and Dermacentor reticulatus (0.3%). Borrelia burgdorferi (s.l.) was detected in 14% of nymphs and adult ticks. Adult ticks (20%) were more likely to be infected than nymphs (12%). The most common genospecies were B. afzelii (52%) and B. garinii (21%). Except for TBEV, the other tick-borne pathogens studied were all detected in the tick sample, although at a lower prevalence: 1.5% for Babesia spp.; 1.8% for A. phagocytophilum; 2.4% for B. miyamotoi; 2.8% for N. mikurensis; and 6.8% for R. helvetica. Rickettsia raoultii, the causative agent of tick-borne lymphadenopathy, was identified for the first time in Belgium, in two out of five D. reticulatus ticks. Co-infections were found in 3.9% of the examined ticks. The most common co-infection was B. burgdorferi (s.l.) + N. mikurensis. Conclusions Although for most of the tick-borne diseases in Belgium, other than Lyme borreliosis, no or few cases of human infection are reported, the pathogens causing these diseases were all (except for TBEV) detected in the tick study sample. Their confirmed presence can help raise awareness among citizens and health professionals in Belgium on possible diseases other than Lyme borreliosis in patients presenting fever or other non-characteristic symptoms after a tick bite.

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