scholarly journals Microarray Analysis of Transcriptional Responses to Abscisic Acid and Salt Stress in Arabidopsis thaliana

2013 ◽  
Vol 14 (5) ◽  
pp. 9979-9998 ◽  
Author(s):  
Yujia Liu ◽  
Xiaoyu Ji ◽  
Lei Zheng ◽  
Xianguang Nie ◽  
Yucheng Wang
Keyword(s):  
Arabidopsis Thaliana ◽  
Abscisic Acid ◽  
Salt Stress ◽  
Microarray Analysis ◽  
Transcriptional Responses

scholarly journals The SAUR41 subfamily of SMALL AUXIN UP RNA genes is abscisic acid inducible to modulate cell expansion and salt tolerance in Arabidopsis thaliana seedlings

2019 ◽  
Vol 125 (5) ◽  
pp. 805-819 ◽  
Author(s):  
Ting Qiu ◽  
Mengyuan Qi ◽  
Xiaohui Ding ◽  
Yanyan Zheng ◽  
Tianjiao Zhou ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Abscisic Acid ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Cell Expansion ◽  
Iron Homeostasis ◽  
Ultrastructural Observation ◽  
Genetic Redundancy ◽  
Transcriptional Responses ◽  
Rna Genes

Abstract Background and Aims Most primary auxin response genes are classified into three families: AUX/IAA, GH3 and SAUR genes. Few studies have been conducted on Arabidopsis thaliana SAUR genes, possibly due to genetic redundancy among different subfamily members. Data mining on arabidopsis transcriptional profiles indicates that the SAUR41 subfamily members of SMALL AUXIN UP RNA genes are, strikingly, induced by an inhibitory phytohormone, abscisic acid (ABA). We aimed to reveal the physiological roles of arabidopsis SAUR41 subfamily genes containing SAUR40, SAUR41, SAUR71 and SAUR72. Methods Transcriptional responses of arabidopsis SAUR41 genes to phytohormones were determined by quantitative real-time PCR. Knock out of SAUR41 genes was carried out with the CRISPR/Cas9 (clustered regulatory interspaced short palindromic repeats/CRISPR-associated protein 9) genome editing technique. The saur41/40/71/72 quadruple mutants, SAUR41 overexpression lines and the wild type were subjected to ultrastructural observation, transcriptome analysis and physiological characterization. Key Results Transcription of arabidopsis SAUR41 subfamily genes is activated by ABA but not by gibberellic acids and brassinosteroids. Quadruple mutations in saur41/40/71/72 led to reduced cell expansion/elongation in cotyledons and hypocotyls, opposite to the overexpression of SAUR41; however, an irregular arrangement of cell size and shape was observed in both cases. The quadruple mutants had increased transcription of calcium homeostasis/signalling genes in seedling shoots, and the SAUR41 overexpression lines had decreased transcription of iron homeostasis genes in roots and increased ABA biosynthesis in shoots. Notably, both the quadruple mutants and the SAUR41 overexpression lines were hypersensitive to salt stress during seedling establishment, whereas specific expression of SAUR41 under the ABA-responsive RD29A (Responsive to Desiccation 29A) promoter in the quadruple mutants rescued the inhibitory effect of salt stress. Conclusions The SAUR41 subfamily genes of arabidopsis are ABA inducible to modulate cell expansion, ion homeostasis and salt tolerance. Our work may provide new candidate genes for improvement of plant abiotic stress tolerance.

Arabidopsis thaliana lipid phosphate phosphatase 2 is involved in abscisic acid signalling in leaves

2011 ◽  
Vol 49 (3) ◽  
pp. 357-362 ◽  
Author(s):  
Sophie Paradis ◽  
Ana Laura Villasuso ◽  
Susana Saez Aguayo ◽  
Régis Maldiney ◽  
Yvette Habricot ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Abscisic Acid ◽  
Lipid Phosphate
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