scholarly journals Erythropoietin Modulates Autophagy Signaling in the Developing Rat Brain in an In Vivo Model of Oxygen-Toxicity

2012 ◽  
Vol 13 (12) ◽  
pp. 12939-12951 ◽  
Author(s):  
Ivo Bendix ◽  
Corina Schulze ◽  
Clarissa von Haefen ◽  
Alexandra Gellhaus ◽  
Stefanie Endesfelder ◽  
...  
Keyword(s):  
Rat Brain ◽  
Oxygen Toxicity ◽  
In Vivo Model ◽  
Developing Rat

scholarly journals Dolichols and proliferating systems.

1994 ◽  
Vol 41 (3) ◽  
pp. 339-344 ◽  
Author(s):  
A Trentalance
Keyword(s):  
Rat Liver ◽  
Total Content ◽  
The Body ◽  
Model Systems ◽  
In Vivo Model ◽  
Dolichyl Phosphate ◽  
Proliferation And Differentiation ◽  
Developing Rat

The results obtained on dolichol metabolism, in two in vivo model systems, the developing rat liver and the regenerating rat liver, which provide different timing and interplay of proliferation and differentiation processes, have been reported. The regenerating liver presents a marked increase of both synthesis and content of dolichol, a decreased cholesterol/dolichol ratio, unchanged synthesis and content of dolichyl phosphate, or dolichol-kinase and dolichyl phosphate-phosphatase activities; no significantly modified distribution of dolichol homologs, with respect to the control. Total content of dolichols is growing during perinatal development. At fetal stages only short chain dolichols are detectable, while the content of dolichyl phosphate is very low and the activity of dolichyl phosphate-phosphatase is high. The study of the role of liver in dolichol supply to the body in the partially hepatectomized rat shows an increased content of dolichol in the blood; blood dolichol is essentially provided by the release from liver and dolichol traffic in the blood is mediated by multiple carriers.

scholarly journals Fas/CD95/APO-1 Can Function as a Death Receptor for Neuronal Cells in Vitro and in Vivo and is Upregulated Following Cerebral Hypoxic-Ischemic Injury to the Developing Rat Brain

2006 ◽  
Vol 10 (1) ◽  
pp. 17-29 ◽  
Author(s):  
Ursula Felderhoff-Mueser ◽  
Deanna L. Taylor ◽  
Kirsty Greenwood ◽  
Mary Kozma ◽  
Dietger Stibenz ◽  
...  
Keyword(s):  
Rat Brain ◽  
Neuronal Cells ◽  
Death Receptor ◽  
Ischemic Injury ◽  
Developing Rat ◽  
Hypoxic Ischemic Injury

scholarly journals Effect of hyperphenylalaninaemia on lipid synthesis from ketone bodies by rat brain

1976 ◽  
Vol 154 (2) ◽  
pp. 319-325 ◽  
Author(s):  
M S. Patel ◽  
O E. Owen
Keyword(s):  
Rat Brain ◽  
Ketone Bodies ◽  
Brain Slices ◽  
Lipid Synthesis ◽  
Coa Transferase ◽  
Old Rats ◽  
And Function ◽  
Developing Rat

The effect of hyperphenylalaninaemia on the metabolism of ketone bodies in vivo and in vitro by developing rat brain was investigated. The incorporation in vivo of [14C]acetoacetate into cerebral lipids was decreased by both chronic (for 3 days) and acute (for 6h) hyperphenylalaninaemia induced by injecting phenylalanine into 1-week-old rats. In studies in vitro it was observed that the incorporation of the radioactivity from [14C]acetoacetate and 3-hydroxy[14C]butyrate into cerebral lipids was inhibited by phenyl-pyruvate, but not by phenylalanine. Phenylpyruvate also inhibited the incorporation of 3H from 3H2O into lipids by brain slices metabolizing either 3-hydroxybutyrate or acetoacetate in the presence of glucose. These findings suggest that the decrease in the incorporation in vivo of [14C]acetoacetate into cerebral lipids in hyperphenylalaninaemic rats is most likely caused by phenylpyruvate and not by phenylalanine. Phenylpyruvate as well as phenylalanine had no inhibitory effects on ketone-body-catabolizing enzymes, namely 3-hydroxybutyrate dehydrogenase, 3-oxo acid CoA-transferase and acetoacetyl-CoA thiolase, in rat brain. Phenylpyruvate but not phenylalanine inhibited the activity of the 2-oxoglutarate dehydrogenase complex from rat and human brain. These findings suggest that the metabolism of ketone bodies is impaired in brains of untreated phenylketonuric patients, and in turn may contribute to the diminution of mental development and function associated with phenylketonuria.

In vivo ethanol decreases phosphorylated MAPK and p70S6 kinase in the developing rat brain

Neuroreport ◽  
2003 ◽  
Vol 14 (10) ◽  
pp. 1395-1399 ◽  
Author(s):  
Ryozo Tsuji ◽  
Marina Guizzetti ◽  
Lucio G. Costa
Keyword(s):  
Rat Brain ◽  
P70s6 Kinase ◽  
Developing Rat

scholarly journals Carbodiimide as a tissue fixative in histamine immunohistochemistry and its application in developmental neurobiology.

1988 ◽  
Vol 36 (3) ◽  
pp. 259-269 ◽  
Author(s):  
P Panula ◽  
O Häppölä ◽  
M S Airaksinen ◽  
S Auvinen ◽  
A Virkamäki
Keyword(s):  
Rat Brain ◽  
Nerve Fibers ◽  
Immunohistochemical Method ◽  
Dot Blot ◽  
Culture Dish ◽  
Long Fibers ◽  
Developing Rat ◽  
Strong Immunoreactivity

The object of this study was to develop an immunohistochemical method that could be used to study neuronal histamine, especially in nerve fibers and terminals where most previous methods have not been applicable. Three new antisera were produced in rabbits against conjugated histamine, and the fixative used in conjugation, 1-ethyl-3(3-diamethylaminopropyl)-carbodiimide (EDCDI), was used in tissue fixation and compared to paraformaldehyde. Specificity of the antisera was established with dot-blot tests on nitrocellulose, with blocking controls and affinity-purified antibodies. EDCDI appeared to be superior to paraformaldehyde as a fixative, and histamine-immunoreactive nerve cells were visualized in developing rat brain during late fetal development from embryonal day 12. By the second postnatal week, the distribution of histamine-immunoreactive neurons in rat brain had reached the adult pattern and immunoreactive nerve fibers were seen in many areas. Posterior hypothalamic neurons from newborn rat in vitro showed strong immunoreactivity for histamine and developed long varicose fibers, which covered the culture dish by the end of the fourth week in vitro. Fixation with EDCDI also allowed detection of histamine in gastric enterochromaffin-like cells and mast cells in rat. The results suggest that the histamine-containing neuron system in rat brain develops during the late fetal and early postnatal periods, and that immunoreactive neurons develop long fibers both in vivo and in vitro.

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