scholarly journals Sequence Analysis and Potentials of the Native RbcS Promoter in the Development of an Alternative Eukaryotic Expression System Using Green Microalga Ankistrodesmus convolutus

2012 ◽  
Vol 13 (3) ◽  
pp. 2676-2691 ◽  
Author(s):  
Tran Thanh ◽  
Vu Thi Quynh Chi ◽  
Hishamuddin Omar ◽  
Mohd Puad Abdullah ◽  
Suhaimi Napis
Keyword(s):  
Sequence Analysis ◽  
Expression System ◽  
Eukaryotic Expression ◽  
Eukaryotic Expression System ◽  
Rbcs Promoter ◽  
Ankistrodesmus Convolutus ◽  
Green Microalga

Cloning and expression of l-asparaginase from E. coli in eukaryotic expression system

2015 ◽  
Vol 102 ◽  
pp. 14-17 ◽  
Author(s):  
Syed Sajitha ◽  
Jalaja Vidya ◽  
karunakaran Varsha ◽  
Parameswaran Binod ◽  
Ashok Pandey
Keyword(s):  
Expression System ◽  
Eukaryotic Expression ◽  
Cloning And Expression ◽  
E Coli ◽  
Eukaryotic Expression System

scholarly journals CRISPR/Cas9-based Antibody Production in Plants

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Bareera Zahoor ◽  
Ummara Waheed ◽  
Shan Saeed ◽  
Fatima Gulzar ◽  
Hira Tasleem ◽  
...  
Keyword(s):  
Transient Expression ◽  
Antibody Production ◽  
Expression System ◽  
Eukaryotic Expression ◽  
Production Plant ◽  
Post Translational Modification ◽  
Eukaryotic Expression System ◽  
Transient Expression System ◽  
Different Types ◽  
Physical And Chemical

Nowadays demand of antibody production is increased to cure different diseases including diabetes, hepatitis and cancer. For that different types of systems are used for the expression of antibody production. But these were not improved the antibody production. Plant cells have several benefits in comparison with other eukaryotic cells if it is considered as eukaryotic expression system. As compared to the human cell or other microorganisms, the plant cell is safe and decrease the contamination of antibody production. In addition, plants perform proper post-translational modification as a eukaryotic expression system. But recently, transient expression system is used due to the safe and improve the quality and quantity of antibody production. In transient expression system agroinfiltration method are mostly used. The main issue in antibody production is purification. Because in downstream process antibody is degraded due to the physical and chemical stresses. These issues can be solved with the help of CRISPR/Cas9. Plant antibody can be tagged with the help of CRISPR/Cas9. This review encompasses the applications of CRISPR technology for producing plant-based antibodies.

Analysis of Synonymous Codon Usage in the US2 Gene of Duck Plague Virus

2013 ◽  
Vol 647 ◽  
pp. 220-226
Author(s):  
Jie Gao ◽  
An Chun Cheng ◽  
Ming Shu Wang
Keyword(s):  
Comparative Analysis ◽  
Codon Usage ◽  
Codon Usage Bias ◽  
Synonymous Codon ◽  
Expression System ◽  
Synonymous Codon Usage ◽  
Eukaryotic Expression ◽  
Eukaryotic Expression System ◽  
Duck Plague Virus

The codon usage bias of DPV-CHv US2 gene (GenBank Accession No. EU195086) will be analysed in this study, and a comparative analysis of DPV-CHv US2 gene and those of other 15 alphaherpesvirus US2 genes was performed. We also analysed the RSCU, ENC, GC3s value of those US2 genes in herpesvirus, some data were analysed specifically in the article. Consequently, we found that the eukaryotic expression system——the yeast is more suitable for the expression of DPV US2 gene.

scholarly journals Analysis of Capsid Formation of Human Polyomavirus JC (Tokyo-1 Strain) by a Eukaryotic Expression System: Splicing of Late RNAs, Translation and Nuclear Transport of Major Capsid Protein VP1, and Capsid Assembly

2000 ◽  
Vol 74 (4) ◽  
pp. 1840-1853 ◽  
Author(s):  
Yukiko Shishido-Hara ◽  
Yoshinobu Hara ◽  
Theresa Larson ◽  
Kotaro Yasui ◽  
Kazuo Nagashima ◽  
...  
Keyword(s):  
Nuclear Transport ◽  
Expression System ◽  
Eukaryotic Expression ◽  
Capsid Proteins ◽  
Human Polyomavirus ◽  
Reading Frame ◽  
Eukaryotic Expression System ◽  
Capsid Formation ◽  
Polyomavirus Jc ◽  
Capsid Protein Vp1

ABSTRACT Human polyomavirus JC (JCV) can encode the three capsid proteins VP1, VP2, and VP3, downstream of the agnoprotein in the late region. JCV virions are identified in the nucleus of infected cells. In this study, we have elucidated unique features of JCV capsid formation by using a eukaryotic expression system. Structures of JCV polycistronic late RNAs (M1 to M4 and possibly M5 and M6) generated by alternative splicing were determined. VP1 would be synthesized from M2 RNA, and VP2 and VP3 would be synthesized from M1 RNA. The presence of the open reading frame of the agnoprotein or the leader sequence (nucleotides 275 to 409) can decrease the expression level of VP1. VP1 was efficiently transported to the nucleus in the presence of VP2 and VP3 but distributed both in the cytoplasm and in the nucleus in their absence. Mutation analysis indicated that inefficiency in nuclear transport of VP1 is due to the unique structure in the N-terminal sequence, KRKGERK. Within the nucleus, VP1 was localized discretely and identified as speckles in the presence of VP2 and VP3 but distributed diffusely in their absence. These results suggest that VP1 was efficiently transported to the nucleus and localized in the discrete subnuclear regions, possibly with VP2 and VP3. By electron microscopy, recombinant virus particles were identified in the nucleus, and their intranuclear distribution was consistent with distribution of speckles. This system provides a useful model with which to understand JCV capsid formation and the structures and functions of the JCV capsid proteins.

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