scholarly journals In vitro Antioxidant of a Water-Soluble Polysaccharide from Dendrobium fimhriatum Hook.var.oculatum Hook

2011 ◽  
Vol 12 (6) ◽  
pp. 4068-4079 ◽  
Author(s):  
Aoxue Luo ◽  
Yijun Fan
Keyword(s):  
Water Soluble ◽  
Soluble Polysaccharide

scholarly journals Characterization and Prebiotic Potential of Longan Juice Obtained by Enzymatic Conversion of Constituent Sucrose into Fructo-Oligosaccharides

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2596 ◽  
Author(s):  
Yongxia Cheng ◽  
Haibo Lan ◽  
Lei Zhao ◽  
Kai Wang ◽  
Zhuoyan Hu
Keyword(s):  
Soluble Sugar ◽  
Monosaccharide Composition ◽  
Water Soluble ◽  
Lactobacillus Delbrueckii ◽  
Soluble Solids ◽  
Enzyme Treatment ◽  
Bacteria Growth ◽  
Before And After ◽  
Soluble Polysaccharide

The prebiotic potential of longan juice obtained by a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide was investigated. The physicochemical properties and carbohydrate composition of the longan juice was evaluated before and after enzymatic treatment. The stimulation effects of the treated longan juice on probiotic bacteria growth were also studied in vitro. The results showed that total soluble solids, yield and clarity of longan juice were all significantly improved after enzyme treatment. The water-soluble polysaccharide content, including pectin, was significantly increased. Compared with the natural longan pulp, the enzyme treated juice showed a significant decrease in sucrose content. Substantial fructo-oligosaccharides including 1-kestose and nystose were synthesized after enzyme treatment. The molecular weight distribution and the monosaccharide composition of the water-soluble polysaccharide were significantly changed by enzyme treatment. The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.

scholarly journals The Effect of Water-Soluble Polysaccharide from Jackfruit (Artocarpus heterophyllus Lam.) on Human Colon Carcinoma Cells Cultured In Vitro

Plants ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 103
Author(s):  
Adrian Wiater ◽  
Roman Paduch ◽  
Sylwia Trojnar ◽  
Adam Choma ◽  
Małgorzata Pleszczyńska ◽  
...  
Keyword(s):  
Biological Activity ◽  
Tumor Cells ◽  
Human Colon ◽  
Carcinoma Cells ◽  
Water Soluble ◽  
Artocarpus Heterophyllus ◽  
Colon Carcinoma Cells ◽  
Human Colon Carcinoma ◽  
Soluble Polysaccharide

Various phytochemical studies have revealed that jackfruit (Artocarpus heterophyllus Lam.) is rich in bioactive compounds, including carotenoids, flavonoids, volatile acids, tannins, and lectins. The aim of the study was to analyze the biological activity of water-soluble polysaccharide (WSP) isolated from jackfruit and to assess its immunomodulatory, cytotoxic, and anti-oxidative effects on human colon carcinoma cells in vitro. The neutral red (NR) uptake assay revealed no toxic influence of the polymer on the viability of tumor cells (HT29 and SW620). After 24 h and 48 h of incubation, the cellular viability was not lower than 94%. The metabolic activity of the cells (MTT) at the compound concentration of 250 µg/mL was higher than 92% in comparison to the control. WSP (250 µg/mL) exerted no significant effect on the morphology of the cells was determined by May-Grünwald-Giemsa staining. WSP changed nitric oxide (NOx) production by the tumor cells depending on the time of incubation and prior 2-h stimulation of the cells with E. coli 0111:B4 LPS. It significantly stimulated IL-1β production by the tumor cells. The IL-6 level increased but that of IL-10 decreased by a WSP concentration-dependent manner. No such effect was detected in SW620. The WSP had antioxidant properties. In conclusion, water-soluble polysaccharide isolated from A. heterophyllus exhibits significant biological activity towards many types of both normal and cancerous cells. Therefore, it may be considered as a useful agent in the protection of human health or in functional and dietary nutrition.

scholarly journals In Vitro and In Vivo Antioxidant Activity of a Water-Soluble Polysaccharide from Dendrobium denneanum

Molecules ◽  
2011 ◽  
Vol 16 (2) ◽  
pp. 1579-1592 ◽  
Author(s):  
Aoxue Luo ◽  
Zhongfu Ge ◽  
Yijun Fan ◽  
Aoshuang Luo ◽  
Ze Chun ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Water Soluble ◽  
Soluble Polysaccharide

scholarly journals Effects of Yeast Mannan Which Promotes Beneficial Bacteroides on the Intestinal Environment and Skin Condition: A Randomized, Double-Blind, Placebo-Controlled Study

Nutrients ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3673
Author(s):  
Reiko Tanihiro ◽  
Katsuhisa Sakano ◽  
Shunsuke Oba ◽  
Chikako Nakamura ◽  
Kohji Ohki ◽  
...  
Keyword(s):  
Skin Condition ◽  
Water Soluble ◽  
Controlled Study ◽  
Double Blind ◽  
Double Blind Placebo ◽  
Intestinal Environment ◽  
Healthy Female ◽  
Soluble Polysaccharide ◽  
Bacteroides Ovatus

Yeast mannan (YM) is an indigestible water-soluble polysaccharide of the yeast cell wall. In vitro fecal fermentation studies showed that YM could exhibit a notable prebiotic effect. The aim of this randomized, double-blind, placebo-controlled study was to assess the efficacy of YM intake on the intestinal environment and skin condition. One hundred and ten healthy female subjects aged 30–49 years were supplemented with YM or placebo for eight weeks. Skin dryness was set as the primary endpoint. No side effects were observed during the study. Microbiota analyses revealed that YM intake selectively increased the relative abundance of Bacteroides thetaiotaomicron and Bacteroides ovatus compared to that by placebo. Feces and urine analyses showed that YM intake lowered the concentration of fecal p-cresol, indole, and skatole, and elevated urinal equol levels compared to those in placebo. Furthermore, YM supplementation ameliorated subjective skin dryness. This study suggests that YM intake could promote beneficial Bacteroides and improve the intestinal environment and skin condition.

Low Molecular Weight Branched Polyethylenimine-Graft-Lentinan as a Novel Synthetic Gene Carrier

2013 ◽  
Vol 815 ◽  
pp. 293-298
Author(s):  
Li Jing Min ◽  
Jing Fen Li
Keyword(s):  
Molecular Weight ◽  
Gel Electrophoresis ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Water Soluble ◽  
Low Molecular Weight ◽  
Dna Migration ◽  
Branched Polyethylenimine ◽  
Soluble Polysaccharide

LMW Branched Polyethylenimine-graft-lentinan was synthesized by performing crosslinker CDI in the presence of water-soluble Polysaccharide (Mw 70,000) and polyethylenimine (PEI, Mw 600). The chemistry of the PEI-g-lentinan obtained were characterized. The results indicated that the amines of lentinan were grafted with PEI. Gel electrophoresis showed that DNA migration was retarded completely at a N/P ratio of 10/1, indicating good DNA condensation capability of PEI-g-lentinan. The cytotoxicity of PEI-g-lentinan was evaluated, and the results reflected that PEI-g-lentinan had a lower cytotoxicity than PEI (25 K). Gene transfection efficiency of PEI-g-chitosan in cos-7 cells was determined. Remarkably,PEI-g-lentinan showed a higher transfection efficiency than that of PEI (25 K) in vitro.

In vivo and in vitro anti-inflammatory effects of water-soluble polysaccharide from Arctium lappa

2019 ◽  
Vol 135 ◽  
pp. 717-724 ◽  
Author(s):  
Nianfeng Zhang ◽  
Yao Wang ◽  
Juan Kan ◽  
Xiaonan Wu ◽  
Xin Zhang ◽  
...  
Keyword(s):  
Water Soluble ◽  
Arctium Lappa ◽  
Soluble Polysaccharide ◽  
Anti Inflammatory

scholarly journals Prebiotic effects of yeast mannan, which selectively promotes Bacteroides thetaiotaomicron and Bacteroides ovatus in a human colonic microbiota model

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shunsuke Oba ◽  
Tadahiro Sunagawa ◽  
Reiko Tanihiro ◽  
Kyoko Awashima ◽  
Hiroshi Sugiyama ◽  
...  
Keyword(s):  
Short Chain Fatty Acids ◽  
Water Soluble ◽  
Pcr Analysis ◽  
Rrna Gene ◽  
Bacteroides Thetaiotaomicron ◽  
Colonic Microbiota ◽  
Gene Sequence Analysis ◽  
Soluble Polysaccharide ◽  
Bacteroides Ovatus

Abstract Yeast mannan (YM) is an indigestible water-soluble polysaccharide of the yeast cell wall, with a notable prebiotic effect on the intestinal microbiota. We previously reported that YM increased Bacteroides thetaiotaomicron abundance in in vitro rat faeces fermentation, concluding that its effects on human colonic microbiota should be investigated. In this study, we show the effects of YM on human colonic microbiota and its metabolites using an in vitro human faeces fermentation system. Bacterial 16S rRNA gene sequence analysis showed that YM administration did not change the microbial diversity or composition. Quantitative real-time PCR analysis revealed that YM administration significantly increased the relative abundance of Bacteroides ovatus and B. thetaiotaomicron. Moreover, a positive correlation was observed between the relative ratio (with or without YM administration) of B. thetaiotaomicron and B. ovatus (r = 0.92), suggesting that these bacteria utilise YM in a coordinated manner. In addition, YM administration increased the production of acetate, propionate, and total short-chain fatty acids. These results demonstrate the potential of YM as a novel prebiotic that selectively increases B. thetaiotaomicron and B. ovatus and improves the intestinal environment. The findings also provide insights that might be useful for the development of novel functional foods.

Structural characterization of novel comb-like branched α-d-glucan from Arca inflata and its immunoregulatory activities in vitro and in vivo

2019 ◽  
Vol 10 (10) ◽  
pp. 6589-6603
Author(s):  
Chunlei Li ◽  
Dan Peng ◽  
Weijuan Huang ◽  
Xiaozheng Ou ◽  
Liyan Song ◽  
...  
Keyword(s):  
Structural Characterization ◽  
Water Soluble ◽  
Soluble Polysaccharide

In the current study, we identified and characterized a novel water-soluble polysaccharide (JNY2PW) with significant immunoregulatory effects and no apparent overall toxicity.

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