scholarly journals Fully Automated On-Chip Imaging Flow Cytometry System with Disposable Contamination-Free Plastic Re-Cultivation Chip

2011 ◽  
Vol 12 (6) ◽  
pp. 3618-3634 ◽  
Author(s):  
Masahito Hayashi ◽  
Akihiro Hattori ◽  
Hyonchol Kim ◽  
Hideyuki Terazono ◽  
Tomoyuki Kaneko ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Imaging Flow Cytometry ◽  
On Chip

scholarly journals On-chip light-sheet fluorescence imaging flow cytometry at a high flow speed of 1 m/s

2018 ◽  
Vol 9 (7) ◽  
pp. 3424 ◽  
Author(s):  
Taichi Miura ◽  
Hideharu Mikami ◽  
Akihiro Isozaki ◽  
Takuro Ito ◽  
Yasuyuki Ozeki ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Fluorescence Imaging ◽  
Light Sheet ◽  
Flow Speed ◽  
High Flow ◽  
Imaging Flow Cytometry ◽  
On Chip

scholarly journals Development of On-Chip Multi-Imaging Flow Cytometry for Identification of Imaging Biomarkers of Clustered Circulating Tumor Cells

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e104372 ◽  
Author(s):  
Hyonchol Kim ◽  
Hideyuki Terazono ◽  
Yoshiyasu Nakamura ◽  
Kazuko Sakai ◽  
Akihiro Hattori ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Imaging Biomarkers ◽  
Imaging Flow Cytometry ◽  
On Chip

On-Chip Multicolor Photoacoustic Imaging Flow Cytometry

2021 ◽  
Author(s):  
Tian Jin ◽  
Chen Zhang ◽  
Fei Liu ◽  
Xingxing Chen ◽  
Guangru Liang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Photoacoustic Imaging ◽  
Imaging Flow Cytometry ◽  
On Chip

scholarly journals Semi-automated classification of colonial Microcystis by FlowCAM imaging flow cytometry in mesocosm experiment reveals high heterogeneity during seasonal bloom

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yersultan Mirasbekov ◽  
Adina Zhumakhanova ◽  
Almira Zhantuyakova ◽  
Kuanysh Sarkytbayev ◽  
Dmitry V. Malashenkov ◽  
...  
Keyword(s):  
Machine Learning ◽  
Flow Cytometry ◽  
Spatial Resolution ◽  
Mesocosm Experiment ◽  
Imaging Flow Cytometry ◽  
Leibler Divergence ◽  
Temporal And Spatial ◽  
High Level ◽  
Training Sets

AbstractA machine learning approach was employed to detect and quantify Microcystis colonial morphospecies using FlowCAM-based imaging flow cytometry. The system was trained and tested using samples from a long-term mesocosm experiment (LMWE, Central Jutland, Denmark). The statistical validation of the classification approaches was performed using Hellinger distances, Bray–Curtis dissimilarity, and Kullback–Leibler divergence. The semi-automatic classification based on well-balanced training sets from Microcystis seasonal bloom provided a high level of intergeneric accuracy (96–100%) but relatively low intrageneric accuracy (67–78%). Our results provide a proof-of-concept of how machine learning approaches can be applied to analyze the colonial microalgae. This approach allowed to evaluate Microcystis seasonal bloom in individual mesocosms with high level of temporal and spatial resolution. The observation that some Microcystis morphotypes completely disappeared and re-appeared along the mesocosm experiment timeline supports the hypothesis of the main transition pathways of colonial Microcystis morphoforms. We demonstrated that significant changes in the training sets with colonial images required for accurate classification of Microcystis spp. from time points differed by only two weeks due to Microcystis high phenotypic heterogeneity during the bloom. We conclude that automatic methods not only allow a performance level of human taxonomist, and thus be a valuable time-saving tool in the routine-like identification of colonial phytoplankton taxa, but also can be applied to increase temporal and spatial resolution of the study.

scholarly journals High-throughput multiparametric imaging flow cytometry: toward diffraction-limited sub-cellular detection and monitoring of sub-cellular processes

Cell Reports ◽  
2021 ◽  
Vol 34 (10) ◽  
pp. 108824
Author(s):  
Gregor Holzner ◽  
Bogdan Mateescu ◽  
Daniel van Leeuwen ◽  
Gea Cereghetti ◽  
Reinhard Dechant ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Multiparametric Imaging ◽  
Cellular Processes ◽  
Imaging Flow Cytometry
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