scholarly journals In Vitro and In Vivo Evaluation of Nanostructured Biphasic Calcium Phosphate in Granules and Putty Configurations

2021 ◽  
Vol 18 (2) ◽  
pp. 533
Author(s):  
Jhonathan R. B. Nascimento ◽  
Suelen C. Sartoretto ◽  
Adriana T. N. N. Alves ◽  
Carlos F. A. B. Mourão ◽  
Victor R. Martinez-Zelaya ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Wistar Rats ◽  
Biphasic Calcium Phosphate ◽  
Biological Response ◽  
Bone Defects ◽  
Fibroblast Cells ◽  
In Vivo Evaluation ◽  
Histological Results

Synthetic biphasic calcium phosphate (BCP) granules and powder are biocompatible biomaterials with a well-known capacity for osteoconduction, presenting very satisfactory clinical and histological results. It remains unanswered if the putty configuration impacts the biological response to the material. In this study, we aimed to compare the cytocompatibility and biocompatibility of nanostructured BCP in the putty configuration (moldable nanostructured calcium phosphate, MnCaP) on the healing of critical-sized bone defects (8 mm) in rat calvaria. Cytocompatibility was determined through the viability of fibroblast cells (V-79) to the extracts of different concentrations of MnCaP. Forty-five Wistar rats were randomly divided into three groups (n = 15)—clot, MnCaP, and commercial biphasic calcium phosphate in granules configurations (Nanosynt®)—and subdivided into three experimental periods (1, 3, and 6 months). Histological, histomorphometric, and microtomographic analyses allowed the evaluation of newly formed bone, residual biomaterial, and connective tissue. The in vitro evaluation showed that MnCaP was cytocompatible. The histomorphometric results showed that the Nanosynt® group granted the highest new-formed bone values at six months (p < 0.05), although the biomaterial volume did not differ between groups. The putty configuration was easier to handle, and both configurations were biocompatible and osteoconductive, presented similar biosorption rates, and preserved the calvaria architecture.

Development of injectable chitosan/biphasic calcium phosphate bone cement and in vitro and in vivo evaluation

2020 ◽  
Vol 15 (5) ◽  
pp. 055038
Author(s):  
Sirirat T. Rattanachan ◽  
Nuan La-ong Srakaew ◽  
Paritat Thaitalay ◽  
Oranich Thongsri ◽  
Rawee Dangviriyakul ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Cement ◽  
Biphasic Calcium Phosphate ◽  
In Vivo Evaluation ◽  
Calcium Phosphate Bone Cement

scholarly journals Biocompatibility and Bone Formation of Flexible, Cotton Wool-like PLGA/Calcium Phosphate Nanocomposites in Sheep

2011 ◽  
Vol 5 (1) ◽  
pp. 63-71 ◽  
Author(s):  
Oliver D Schneider ◽  
Dirk Mohn ◽  
Roland Fuhrer ◽  
Karina Klein ◽  
Käthi Kämpf ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Antimicrobial Properties ◽  
Drill Hole ◽  
Bone Defects ◽  
Long Bone ◽  
Minimal Amount ◽  
Cotton Wool

Background: The purpose of this preliminary study was to assess the in vivo performance of synthetic, cotton wool-like nanocomposites consisting of a biodegradable poly(lactide-co-glycolide) fibrous matrix and containing either calcium phosphate nanoparticles (PLGA/CaP 60:40) or silver doped CaP nanoparticles (PLGA/Ag-CaP 60:40). Besides its extraordinary in vitro bioactivity the latter biomaterial (0.4 wt% total silver concentration) provides additional antimicrobial properties for treating bone defects exposed to microorganisms. Materials and Methods: Both flexible artificial bone substitutes were implanted into totally 16 epiphyseal and metaphyseal drill hole defects of long bone in sheep and followed for 8 weeks. Histological and histomorphological analyses were conducted to evaluate the biocompatibility and bone formation applying a score system. The influence of silver on the in vivo performance was further investigated. Results: Semi-quantitative evaluation of histology sections showed for both implant materials an excellent biocompatibility and bone healing with no resorption in the adjacent bone. No signs of inflammation were detectable, either macroscopically or microscopically, as was evident in 5 µm plastic sections by the minimal amount of inflammatory cells. The fibrous biomaterials enabled bone formation directly in the centre of the former defect. The area fraction of new bone formation as determined histomorphometrically after 8 weeks implantation was very similar with 20.5 ± 11.2 % and 22.5 ± 9.2 % for PLGA/CaP and PLGA/Ag-CaP, respectively. Conclusions: The cotton wool-like bone substitute material is easily applicable, biocompatible and might be beneficial in minimal invasive surgery for treating bone defects.

scholarly journals The possibility of healing alveolar bone defects with simvastatin thermosensitive gel: in vitro/in vivo evaluation

2018 ◽  
Vol Volume 12 ◽  
pp. 1997-2003 ◽  
Author(s):  
Hong Ruan ◽  
Youcheng Yu ◽  
Xuehua Guo ◽  
Qian Jiang ◽  
Ying Luo
Keyword(s):  
Alveolar Bone ◽  
Bone Defects ◽  
In Vivo Evaluation ◽  
Thermosensitive Gel

Biocompatibility of Surface-Modified Biphasic Calcium Phosphate/Poly-L-Lactide Biocomposite in vitro and in vivo

2010 ◽  
Vol 26 (8) ◽  
pp. 754-758 ◽  
Author(s):  
Weizhong Yang ◽  
Guangfu Yin ◽  
Dali Zhou ◽  
Jianwen Gu ◽  
Yadong Li ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Biphasic Calcium Phosphate ◽  
Surface Modified

In vivo evaluation of interactions between biphasic calcium phosphate (BCP)-niobium pentoxide (Nb2O5) nanocomposite and tissues using a rat critical-size calvarial defect model

2020 ◽  
Vol 31 (8) ◽  
Author(s):  
Helio de Jesus Kiyochi Junior ◽  
Aline Gabriela Candido ◽  
Taiana Gabriela Moretti Bonadio ◽  
José Adauto da Cruz ◽  
Mauro Luciano Baesso ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Biphasic Calcium Phosphate ◽  
Critical Size ◽  
Niobium Pentoxide ◽  
Calvarial Defect ◽  
Defect Model ◽  
In Vivo Evaluation

In vitro and in vivo evaluation of the biocompatibility of a calcium phosphate/poly(lactic-co-glycolic acid) composite

2012 ◽  
Vol 23 (7) ◽  
pp. 1785-1796 ◽  
Author(s):  
A. Gala-García ◽  
M. B. H. Carneiro ◽  
G. A. B. Silva ◽  
L. S. Ferreira ◽  
L. Q. Vieira ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Glycolic Acid ◽  
In Vivo Evaluation

Biphasic calcium phosphate to repair nasal septum: The first in vitro and in vivo study

2010 ◽  
Vol 6 (3) ◽  
pp. 909-919 ◽  
Author(s):  
Ludovic de Gabory ◽  
Reine Bareille ◽  
Dominique Stoll ◽  
Laurence Bordenave ◽  
Jean-Christophe Fricain
Keyword(s):  
Calcium Phosphate ◽  
Nasal Septum ◽  
Biphasic Calcium Phosphate ◽  
In Vivo Study

scholarly journals Surface mineralized biphasic calcium phosphate ceramics loaded with urine-derived stem cells are effective in bone regeneration

2019 ◽  
Vol 14 (1) ◽  
Author(s):  
Fei Xing ◽  
Lang Li ◽  
Jiachen Sun ◽  
Guoming Liu ◽  
Xin Duan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Calcium Phosphate ◽  
Bone Tissue ◽  
Biphasic Calcium Phosphate ◽  
Bone Defects ◽  
Biological Characteristics ◽  
Segmental Bone ◽  
Segmental Bone Defects

Abstract Background Segmental bone defects caused by trauma, tumors, or infection are a serious challenge for orthopedists in the world. Recent developments in tissue engineering have provided a new treatment for segmental bone defects. Urine-derived stem cells (USCs) can be obtained noninvasively and might be a new kind of seed cells used in bone tissue regeneration. Therefore, the first aim of the present study was to investigate the biological characteristics of USCs. The second aim of the present study was to study the osteogenic effect of surface mineralized biphasic calcium phosphate ceramics (BCPs) loaded with USCs in vitro and in vivo. Methods We isolated USCs from the urine of healthy adult donors and evaluated the biological characteristics of USCs in vitro. We mineralized the surface of BCPs by simulated body fluid (SBF). Cell adhesion and proliferation of USCs on the surface mineralized BCPs were evaluated. Osteogenic proteins and genes of USCs on the surface mineralized BCPs were texted by enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR) assay. Critical-sized segmental bone defects model in New Zealand white rabbits were established and randomly divided into 4 groups (surface mineralized BCPs loaded with USCs, BCPs loaded with USCs, surface mineralized BCPs, and BCPs) based on the implant they received. The therapeutic efficacy of the scaffolds in a large bone defect at post-implantation was evaluated by imaging and histological examination. Results USCs isolated in our study expressed stem cell-specific phenotypes and had a stable proliferative capacity and multipotential differentiation capability. Surface mineralized BCPs promoted osteogenic proteins and genes expression of USCs without affecting the proliferation of USCs. After 10 weeks, the amount of new bone formation was the highest in the group of surface mineralized BCPs loaded with USCs. Conclusion USCs, from non-invasive sources, have good application prospects in the field of bone tissue engineering. Surface mineralized BCPs can significantly enhance osteogenic potential of USCs without changing biological characteristics of BCPs. Surface mineralized BCPs loaded with USCs are effective in repairing of critical-sized segmental bone defects in rabbits.

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