scholarly journals Use of a Sampling Area-Adjusted Adenosine Triphosphate Bioluminescence Assay Based on Digital Image Quantification to Assess the Cleanliness of Hospital Surfaces

2016 ◽  
Vol 13 (6) ◽  
pp. 576 ◽  
Author(s):  
Yu-Huai Ho ◽  
Lih-Shinn Wang ◽  
Hui-Li Jiang ◽  
Chih-Hui Chang ◽  
Chia-Jung Hsieh ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Digital Image ◽  
Image Quantification ◽  
Sampling Area ◽  
Bioluminescence Assay

scholarly journals Effectiveness of Surface Cleaning and Disinfection in a Brazilian Healthcare Facility

2018 ◽  
Vol 12 (1) ◽  
pp. 36-44 ◽  
Author(s):  
Aires G. Santos-Junior ◽  
Adriano M. Ferreira ◽  
Oleci P. Frota ◽  
Marcelo A. Rigotti ◽  
Larissa da S. Barcelos ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Adenosine Triphosphate ◽  
Healthcare Facility ◽  
Surface Cleaning ◽  
Point Of View ◽  
Colony Count ◽  
Before And After ◽  
Bioluminescence Assay ◽  
Cleaning And Disinfection ◽  
Hospital Cleaning

Background: Failures in the processes of cleaning and disinfecting health service surfaces may result in the spread and transfer of pathogens that are often associated with healthcare-related infections and outbreaks. Aims: To assess the effectiveness of environmental surface cleaning and disinfection in a hospital clinic. Method: The study was conducted in a nursing ward with 45 beds. A total of 80 samples from five high-touch surfaces were evaluated before and after cleaning and disinfection, using the following methods: visual inspection, adenosine triphosphate bioluminescence assay, aerobic colony count, Staphylococcus aureus colony count, and evaluation of resistance to methicillin. The data analysis used nonparametric comparative and correlative tests to observe any differences in the pre- and post- cleaning and disinfection results for the surfaces assessed. Results: Effective cleaning and disinfection had a significant effect on only two surfaces when measured for the presence of adenosine triphosphate, the inner bathroom door handle (p=0.007) and the toilet bowl (p=0.01). When evaluated for Staphylococcus aureus colony count, the toilet flush handle also demonstrated a significant effect (p=0.04). Conclusion: The effectiveness of cleaning and disinfection of the surfaces tested was not satisfactory. An educational intervention is recommended for the cleaning and disinfection staff and the nursing team at the healthcare facility. Relevance to Clinical Practice: The data in the study revealed that daily hospital cleaning and disinfection in the sampled sites are not sufficient in medical and surgical wards. Hospital cleanliness must be reevaluated from the point of view of materials, such as an adequate supply of clean cloths, in addition to establishing more precise cleanliness protocols and accurate monitoring systems.

scholarly journals Evaluating the efficacy of environmental cleanliness by adenosine triphosphate bioluminescence assay at a medical center

2015 ◽  
Vol 48 (2) ◽  
pp. S55
Author(s):  
Yu-Shan Huang ◽  
I-Chen Hung ◽  
Mei-Ling Chen ◽  
Jann-Tay Wang ◽  
Wang-Huei Sheng ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Medical Center ◽  
Bioluminescence Assay

Assessment of an innovative antimicrobial surface disinfectant in the operating room environment using adenosine triphosphate bioluminescence assay

2015 ◽  
Vol 43 (3) ◽  
pp. 283-285 ◽  
Author(s):  
Brian D. Lewis ◽  
Maureen Spencer ◽  
Peter J. Rossi ◽  
Cheong J. Lee ◽  
Kellie R. Brown ◽  
...  
Keyword(s):  
Operating Room ◽  
Adenosine Triphosphate ◽  
Bioluminescence Assay

Comparison of Fluorescent Marker Systems with 2 Quantitative Methods of Assessing Terminal Cleaning Practices

2011 ◽  
Vol 32 (12) ◽  
pp. 1187-1193 ◽  
Author(s):  
John M. Boyce ◽  
Nancy L. Havill ◽  
Heather L. Havill ◽  
Elise Mangione ◽  
Diane G. Dumigan ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Quantitative Methods ◽  
Convenience Sample ◽  
Fluorescent Marker ◽  
Fluorescent Markers ◽  
Before And After ◽  
Atp Bioluminescence ◽  
Bioluminescence Assay ◽  
Atp Bioluminescence Assay ◽  
Community Teaching

Objective.To compare fluorescent markers with aerobic colony counts (ACCs) and an adenosine triphosphate (ATP) bioluminescence assay system for assessing terminal cleaning practices.Design.A prospective observational survey.Setting.A 500-bed university-affiliated community teaching hospital.Methods.In a convenience sample of 100 hospital rooms, 5 high-touch surfaces were marked with fluorescent markers before terminal cleaning and checked after cleaning to see whether the marker had been entirely or partially removed. ACC and ATP readings were performed on the same surfaces before and after terminal cleaning.Results.Overall, 378 (76%) of 500 surfaces were classified as having been cleaned according to fluorescent markers, compared with 384 (77%) according to ACC criteria and 225 (45%) according to ATP criteria. Of 382 surfaces classified as not clean according to ATP criteria before terminal cleaning, those with the marker removed were significantly more likely than those with the marker partially removed to be classified as clean according to ATP criteria (P = .003).Conclusions.Fluorescent markers are useful in determining how frequently high-touch surfaces are wiped during terminal cleaning. However, contaminated surfaces classified as clean according to fluorescent marker criteria after terminal cleaning were significantly less likely to be classified as clean according to ACC and ATP assays.

scholarly journals Variability of Adenosine Triphosphate–Based Bioluminescence Assay Readings among Drug-Resistant Pathogens

2012 ◽  
Vol 33 (12) ◽  
pp. 1286-1288 ◽  
Author(s):  
Michaela Heller ◽  
Paul A. Thompson ◽  
Mark H. Loock ◽  
Ander Sawchuk ◽  
Dubert M. Guerrero
Keyword(s):  
Adenosine Triphosphate ◽  
Drug Resistant ◽  
Bioluminescence Assay

scholarly journals Investigation of the Cleanliness of Hospital Environmental Surfaces by Adenosine Triphosphate Bioluminescence Assay

2014 ◽  
Vol 29 (6) ◽  
pp. 417-423 ◽  
Author(s):  
Hirofumi SHIBATA ◽  
Kazuyoshi KAWAZOE ◽  
Takahiro SHIBATA ◽  
Shuji FUSHITANI ◽  
Miho WATANABE ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Environmental Surfaces ◽  
Bioluminescence Assay
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