scholarly journals Influence of the Ovine Genital Tract Microbiota on the Species Artificial Insemination Outcome. A Pilot Study in Commercial Sheep Farms

2020 ◽  
Vol 9 (3) ◽  
pp. 16
Author(s):  
Malena Serrano ◽  
Eric Climent ◽  
Fernando Freire ◽  
Juan F. Martínez-Blanch ◽  
Carmen González ◽  
...  
Keyword(s):  
Pilot Study ◽  
16S Rrna ◽  
Antibiotic Treatment ◽  
Artificial Insemination ◽  
Genital Tract ◽  
Reproductive Disorders ◽  
Hypervariable Regions ◽  
Histophilus Somni ◽  
Outcome Differences ◽  
First Time

To date, there is a lack of research into the vaginal and sperm microbiome and its bearing on artificial insemination (AI) success in the ovine species. Using hypervariable regions V3–V4 of the 16S rRNA, we describe, for the first time, the combined effect of the ovine microbiome of both females (50 ewes belonging to five herds) and males (five AI rams from an AI center) on AI outcome. Differences in microbiota abundance between pregnant and non-pregnant ewes and between ewes carrying progesterone-releasing intravaginal devices (PRID) with or without antibiotic were tested at different taxonomic levels. The antibiotic treatment applied with the PRID only altered Streptobacillus genus abundance, which was significantly lower in ewes carrying PRID with antibiotic. Mageebacillus, Histophilus, Actinobacilllus and Sneathia genera were significantly less abundant in pregnant ewes. In addition, these genera were more abundant in two farms with higher AI failure. Species of these genera such as Actinobacillus seminis and Histophilus somni have been associated with reproductive disorders in the ovine species. These genera were not present in the sperm samples of AI rams, but were found in the foreskin samples of rams belonging to herd 2 (with high AI failure rate) indicating that their presence in ewes’ vagina could be due to prior transmission by natural mating with rams reared in the herd.

scholarly journals Microbiome of the lower genital tract in Chinese women with endometriosis by 16s-rRNA sequencing technique: a pilot study

2020 ◽  
Vol 8 (21) ◽  
pp. 1440-1440
Author(s):  
Sikai Chen ◽  
Zhiyue Gu ◽  
Wen Zhang ◽  
Shuangzheng Jia ◽  
Yushi Wu ◽  
...  
Keyword(s):  
Pilot Study ◽  
16S Rrna ◽  
Genital Tract ◽  
Chinese Women ◽  
16S Rrna Sequencing ◽  
Lower Genital Tract ◽  
Sequencing Technique ◽  
Rrna Sequencing

Mother's MILK Messaging: A PILOT Study of an APP to Support Breastfeeding in First Time Mothers

PEDIATRICS ◽  
2016 ◽  
Vol 137 (Supplement 3) ◽  
pp. 218A-218A ◽  
Author(s):  
Maya Bunik ◽  
Jennifer Leifermann ◽  
Jessica R. Ryan ◽  
Anna Furniss ◽  
Sheana Bull
Keyword(s):  
Pilot Study ◽  
First Time Mothers ◽  
Mother's Milk ◽  
Mother’S Milk ◽  
First Time

scholarly journals Molecular Detection and Phylogeny of Anaplasmaphagocytophilum and Related Variants in Small Ruminants from Turkey

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 814
Author(s):  
Münir Aktaş ◽  
Sezayi Özübek ◽  
Mehmet Can Uluçeşme
Keyword(s):  
16S Rrna ◽  
Small Ruminants ◽  
Rrna Gene ◽  
Infection Rates ◽  
Sheep And Goats ◽  
Significant Difference ◽  
Pcr Rflp ◽  
First Time ◽  
Japan And China ◽  
Apparently Healthy

Anaplasma phagocytophilum causes tick-borne fever in small ruminants. Recently, novel Anaplasma variants related to A. phagocytophilum have been reported in ruminants from Tunisia, Italy, South Korea, Japan, and China. Based on 16S rRNA and groEL genes and sequencing, we screened the frequency of A. phagocytophilum and related variants in 433 apparently healthy small ruminants in Turkey. Anaplasma spp. overall infection rates were 27.9% (121/433 analyzed samples). The frequency of A. phagocytophilum and A. phagocytophilum-like 1 infections was 1.4% and 26.5%, respectively. No A. phagocytophilum-like 2 was detected in the tested animals. The prevalence of Anaplasma spp. was comparable in species, and no significant difference was detected between sheep and goats, whereas the prevalence significantly increased with tick infestation. Sequencing confirmed PCR-RFLP data and showed the presence of A. phagocytophilum and A. phagocytophilum-like-1 variant in the sampled animals. Phylogeny-based on 16S rRNA gene revealed the A. phagocytophilum-like 1 in a separate clade together with the previous isolates detected in small ruminants and ticks. In this work, A. phagocytophilum-like 1 has been detected for the first time in sheep and goats from Turkey. This finding revealed that the variant should be considered in the diagnosis of caprine and ovine anaplasmosis.

scholarly journals A pilot study on the impact of a first-time central heating intervention on resident mental wellbeing

2020 ◽  
pp. 1420326X2097546
Author(s):  
Richard A Sharpe ◽  
Andrew J Williams ◽  
Ben Simpson ◽  
Gemma Finnegan ◽  
Tim Jones
Keyword(s):  
Pilot Study ◽  
Indoor Environment ◽  
Intervention Group ◽  
Well Being ◽  
Waiting List ◽  
Mental Wellbeing ◽  
Central Heating ◽  
The Impact ◽  
First Time ◽  
Mental Well Being

Fuel poverty affects around 34% of European homes, representing a considerable burden to society and healthcare systems. This pilot study assesses the impact of an intervention to install a new first time central heating system in order to reduce fuel poverty on household satisfaction with indoor temperatures/environment, ability to pay bills and mental well-being. In Cornwall, 183 households received the intervention and a further 374 went onto a waiting list control. A post-intervention postal questionnaires and follow-up phone calls were undertaken ( n = 557) to collect data on household demographics, resident satisfaction with indoor environment, finances and mental well-being (using the Short Warwick-Edinburgh Mental Wellbeing scale). We compared responses between the waiting list control and intervention group to assess the effectiveness of the intervention. A total of 31% of participants responded, 83 from the waiting list control and 71 from the intervention group. The intervention group reported improvements in the indoor environment, finances and mental well-being. However, these benefits were not expressed by all participants, which may result from diverse resident behaviours, lifestyles and housing characteristics. Future policies need to consider whole house approaches alongside resident training and other behaviour change techniques that can account for complex interactions between behaviours and the built environment.

scholarly journals First record of Ornithodoros faccinii (Acari: Argasidae) on toads of genus Rhinella (Anura: Bufonidae) in Brazil

2018 ◽  
Author(s):  
Hermes Ribeiro Luz ◽  
Bruna Barboza Bezerra ◽  
Walter Flausino ◽  
Arlei Marcili ◽  
Sebastián Muñoz-Leal ◽  
...  
Keyword(s):  
16S Rrna ◽  
Tick Species ◽  
Rio De Janeiro ◽  
Bird Species ◽  
First Record ◽  
Molecular Approach ◽  
Phylogenetic Relatedness ◽  
Mitochondrial Sequence ◽  
First Time

Abstract Although a group of soft ticks (Argasidae) associated with amphibians was recently discovered in Brazilian rainforests, parasitism by these ticks on cold-blooded animals remains less common than on mammal and bird species. In this study, we identified ticks that were collected from toads that had been caught in December 2016 and January 2017, at Itinguçú waterfall (22°54’05” S; 43°53’30” W) in the municipality of Itaguaí, state of Rio de Janeiro. Tick specimens were identified using a morphological and molecular approach. In total, twelve larvae of Ornithodoros ticks were collected from three individuals of Rhinella ornata and were identified as Ornithodoros faccinii. Our results include a longer 16S rRNA mitochondrial sequence for O. faccinii that supports its phylogenetic relatedness to Ornithodoros saraivai, and we report this tick species parasitizing Rhinella toads for the first time in Brazil.

scholarly journals Microbial Diversity Profiling of Gut Microbiota of Macropus giganteus Using Three Hypervariable Regions of the Bacterial 16S rRNA

2021 ◽  
Vol 9 (8) ◽  
pp. 1721
Author(s):  
Christian O’Dea ◽  
Roger Huerlimann ◽  
Nicole Masters ◽  
Anna Kuballa ◽  
Cameron Veal ◽  
...  
Keyword(s):  
16S Rrna ◽  
Gut Microbiota ◽  
Microbial Diversity ◽  
Surface Waters ◽  
Bacterial Species ◽  
Rrna Gene ◽  
Faecal Contamination ◽  
Hypervariable Regions ◽  
Geographical Regions ◽  
V3 Region

Animal faecal contamination of surface waters poses a human health risk, as they may contain pathogenic bacteria or viruses. Of the numerous animal species residing along surface waterways in Australia, macropod species are a top contributor to wild animals’ faecal pollution load. We characterised the gut microbiota of 30 native Australian Eastern Grey Kangaroos from six geographical regions (five kangaroos from each region) within South East Queensland in order to establish their bacterial diversity and identify potential novel species-specific bacteria for the rapid detection of faecal contamination of surface waters by these animals. Using three hypervariable regions (HVRs) of the 16S rRNA gene (i.e., V1–V3, V3–V4, and V5–V6), for their effectiveness in delineating the gut microbial diversity, faecal samples from each region were pooled and microbial genomic DNA was extracted, sequenced, and analysed. Results indicated that V1-V3 yielded a higher taxa richness due to its larger target region (~480 bp); however, higher levels of unassigned taxa were observed using the V1-V3 region. In contrast, the V3–V4 HVR (~569 bp) attained a higher likelihood of a taxonomic hit identity to the bacterial species level, with a 5-fold decrease in unassigned taxa. There were distinct dissimilarities in beta diversity between the regions, with the V1-V3 region displaying the highest number of unique taxa (n = 42), followed by V3–V4 (n = 11) and V5–V6 (n = 8). Variations in the gut microbial diversity profiles of kangaroos from different regions were also observed, which indicates that environmental factors may impact the microbial development and, thus, the composition of the gut microbiome of these animals.

scholarly journals The Origin of Plasma-Derived Bacterial Extracellular Vesicles in Healthy Individuals and Patients with Inflammatory Bowel Disease: A Pilot Study

Genes ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1636
Author(s):  
Emily Jones ◽  
Régis Stentz ◽  
Andrea Telatin ◽  
George M. Savva ◽  
Catherine Booth ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
Pilot Study ◽  
16S Rrna ◽  
Gene Sequencing ◽  
16S Rrna Gene Sequencing ◽  
Rrna Gene ◽  
Healthy Individuals ◽  
Inflammatory Bowel ◽  
Rrna Gene Sequencing ◽  
Blood Microbiota

The gastrointestinal tract harbors the gut microbiota, structural alterations of which (dysbiosis) are linked with an increase in gut permeability (“leaky gut”), enabling luminal antigens and bacterial products such as nanosized bacterial extracellular vesicles (BEVs) to access the circulatory system. Blood-derived BEVs contain various cargoes and may be useful biomarkers for diagnosis and monitoring of disease status and relapse in conditions such as inflammatory bowel disease (IBD). To progress this concept, we developed a rapid, cost-effective protocol to isolate BEV-associated DNA and used 16S rRNA gene sequencing to identify bacterial origins of the blood microbiome of healthy individuals and patients with Crohn’s disease and ulcerative colitis. The 16S rRNA gene sequencing successfully identified the origin of plasma-derived BEV DNA. The analysis showed that the blood microbiota richness, diversity, or composition in IBD, healthy control, and protocol control groups were not significantly distinct, highlighting the issue of ‘kit-ome’ contamination in low-biomass studies. Our pilot study provides the basis for undertaking larger studies to determine the potential use of blood microbiota profiling as a diagnostic aid in IBD.

scholarly journals Morphologic aspects of the genital tract from Nellore cows undergoing gynecological screening to insertion in the fixed-time artificial insemination (TAI) program

2014 ◽  
Vol 31 (02) ◽  
pp. 118-122
Author(s):  
R. Mello ◽  
M. Mello ◽  
M. Abidu-Figueiredo ◽  
P. Scherer ◽  
H. Palhano
Keyword(s):  
Artificial Insemination ◽  
Genital Tract ◽  
Body Condition Score ◽  
Fixed Time ◽  
The Body ◽  
Morphological Aspects ◽  
Functional Aspects ◽  
Condition Score ◽  
The Right ◽  
Rectal Palpation

Abstract Introduction: This study aimed to evaluate the morphology of the genital tract from 98 Nellore cows by rectal palpation and combine them with the functional aspects for inclusion in a fixed-time artificial insemination (TAI) program. Methods: Were evaluated the ovaries, cervix and vulva, as well as the body condition score (BCS scale 1-5). Results: In relation to the ovaries, there were palpable structures found in 51 (17 CL and 34 FL) in the rights ovarian and 37 palpable structures (06 CL and 31 FL) in the left ovary. Asymmetry was found in 17.3% and uterine cervicitis in 20.4% of examined females. Changes were noted in vulva in 51.0% (40 to 10 papules and hyperemia) and the average of BCS was 3.15. On the basis of morphological aspects founded, 29 females with an average of BCS 2.7 and 01 with metritis were excluded and 07 (BCS-3, 5) inseminated immediately after the exam, with 61 included in the TAI program. These data support the conclusion that of all ovulations occurred, characterized by the presence of CL, most occurred in the right ovary (73.9%). The prevalence rate (51%) of vulvar aspects found, indicate a need for research of reproductive diseases in their flock. Conclusion: The BCS may impacts on the cyclicity and in the pregnancy rate of females included in the program.

scholarly journals A pilot study of the effect of deployment on the gut microbiome and traveler’s diarrhea susceptibility

2020 ◽  
Author(s):  
Blake W. Stamps ◽  
Wanda J. Lyon ◽  
Adam P. Irvin ◽  
Nancy Kelley-Loughnane ◽  
Michael S. Goodson
Keyword(s):  
Pilot Study ◽  
16S Rrna ◽  
Gut Microbiome ◽  
16S Rrna Gene Sequencing ◽  
Initial Study ◽  
Mitigation Strategies ◽  
Taxonomic Resolution ◽  
Rrna Gene ◽  
Traveler’S Diarrhea ◽  
Traveler's Diarrhea

AbstractTraveler’s diarrhea (TD) is a recurrent and significant issue for many travelers including the military. While many known enteric pathogens exist that are causative agents of diarrhea, our gut microbiome may also play a role in travelers’ diarrhea susceptibility. To this end we conducted a pilot study of the microbiome of warfighters prior to- and after deployment overseas to identify marker taxa relevant to traveler’s diarrhea. This initial study utilized full-length 16S rRNA gene sequencing to provide additional taxonomic resolution towards identifying predictive taxa.16S rRNA analyses of pre- and post-deployment fecal samples identified multiple marker taxa as significantly differentially abundant in subjects that reported diarrhea, including Weissella, Butyrivibrio, Corynebacterium, uncultivated Erysipelotrichaceae, Jeotgallibaca, unclassified Ktedonobacteriaceae, Leptolinea, and uncultivated Ruminiococcaceae. The ability to identify TD risk prior to travel will inform prevention and mitigation strategies to influence diarrhea susceptibility while traveling.

scholarly journals Non-specific Bacterial Profiles in Reproductive Tract of Dairy Cattle during Artificial Insemination

2021 ◽  
Vol 11 (1) ◽  
pp. 110-114
Author(s):  
Astin Islam Andriani ◽  
Sri Pantja Madyawati ◽  
Emy Koestanti Sabdoningrum
Keyword(s):  
Dairy Cattle ◽  
Bacterial Infection ◽  
Artificial Insemination ◽  
Reproductive Tract ◽  
The Other ◽  
Reproductive Efficiency ◽  
Reproductive Disorders ◽  
Bacterial Isolation ◽  
Isolation And Identification ◽  
Other Hand

One of the causes of low reproductive efficiency in dairy cattle is the presence of reproductive disorders caused by a non-specific bacterial infection. The aim of the present study was to isolate and identify the genus of non-specific bacteria in the reproductive tract of dairy cattle during artificial insemination. A total of 10 samples in the form of mucus attached to the plastic sheath used after artificial insemination in dairy cattle were collected in the study. The samples were subjected to bacterial isolation and identification. The obtained results of the study indicated that Staphylococcus was the dominant genus found (90%). On the other hand, other genera were Escherichia (60%) and Corynebacterium (20%).

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