scholarly journals The drnf1 Gene from the Drought-Adapted Cyanobacterium Nostoc flagelliforme Improved Salt Tolerance in Transgenic Synechocystis and Arabidopsis Plant

Genes ◽  
2018 ◽  
Vol 9 (9) ◽  
pp. 441 ◽  
Author(s):  
Lijuan Cui ◽  
Yinghui Liu ◽  
Yiwen Yang ◽  
Shuifeng Ye ◽  
Hongyi Luo ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Candidate Gene ◽  
Abiotic Stress Tolerance ◽  
Sugar Metabolism ◽  
Limiting Factors ◽  
Model Organisms ◽  
Nostoc Flagelliforme ◽  
Salt Tolerant ◽  
Transgenic Organisms ◽  
Biotic Resource

Environmental abiotic stresses are limiting factors for less tolerant organisms, including soil plants. Abiotic stress tolerance-associated genes from prokaryotic organisms are supposed to have a bright prospect for transgenic application. The drought-adapted cyanobacterium Nostoc flagelliforme is arising as a valuable prokaryotic biotic resource for gene excavation. In this study, we evaluated the salt-tolerant function and application potential of a candidate gene drnf1 from N. flagelliforme, which contains a P-loop NTPase (nucleoside-triphosphatase) domain, through heterologous expression in two model organisms Synechocystis sp. PCC 6803 and Arabidopsis thaliana. It was found that DRNF1 could confer significant salt tolerance in both transgenic organisms. In salt-stressed transgenic Synechocystis, DRNF1 could enhance the respiration rate; slow-down the accumulation of exopolysaccharides; up-regulate the expression of salt tolerance-related genes at a higher level, such as those related to glucosylglycerol synthesis, Na+/H+ antiport, and sugar metabolism; and maintain a better K+/Na+ homeostasis, as compared to the wild-type strain. These results imply that DRNF1 could facilitate salt tolerance by affecting the respiration metabolism and indirectly regulating the expression of important salt-tolerant genes. Arabidopsis was employed to evaluate the salt tolerance-conferring potential of DRNF1 in plants. The results show that it could enhance the seed germination and shoot growth of transgenic plants under saline conditions. In general, a novel prokaryotic salt-tolerant gene from N. flagelliforme was identified and characterized in this study, enriching the candidate gene pool for genetic engineering in plants.

scholarly journals Selection of sunflower genotypes for salt stress and mechanisms of salt tolerance in contrasting genotypes

2020 ◽  
Vol 44 ◽  
Author(s):  
André Dias de Azevedo Neto ◽  
Katia Núbia Azevedo Barros Mota ◽  
Petterson Costa Conceição Silva ◽  
Alide Mitsue Watanabe Cova ◽  
Rogério Ferreira Ribas ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Biomass Production ◽  
Limiting Factors ◽  
Salt Tolerant ◽  
Breeding Programs ◽  
High K ◽  
Species Characteristics ◽  
Mechanisms Of Salt Tolerance ◽  
Lower Biomass

ABSTRACT Salinity is one of the main limiting factors for crop growth. The metabolic responses to salt stress are variable and depend on species characteristics. This study aimed to select sunflower genotypes tolerant to salt stress and evaluate some mechanisms of salt tolerance in two contrasting (salt-tolerant and salt-sensitive) genotypes. In the first assay, the biomass production and the accumulation of Na+ and K+ in 26 sunflower genotypes were evaluated. Genotypes AG963, AG967, AG972, BRS321, BRS324, H251, H360 and H863 showed lower biomass production and were characterized as salt-sensitive and the genotypes BRS323, Catisol, EXP11-26, EXP44-49, EXP60050, EXP887, HLA860HO and Olisun 5 showed higher biomass production and were considered salt-tolerant. The high K+ content and the low Na+ content in the leaves were the ion traits related to salt tolerance and can be used in sunflower breeding programs for this purpose. In the second assay, the plants of salt-tolerant BRS323 had lower Na+ and Cl- contents and higher levels of K+ than plants of salt-sensitive AG967. A better homeostasis in the mechanisms of transport, distribution and accumulation of inorganic solutes in conjunction with a more efficient osmoregulation mechanism through the synthesis of organic solutes may, at least in part, explain the greater salt-tolerance of BRS323 genotype in comparison to AG967.

scholarly journals Identification and Characterization of Wheat Germplasm for Salt Tolerance

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 268
Author(s):  
Xiaoyan Quan ◽  
Xiaoli Liang ◽  
Hongmei Li ◽  
Chunjuan Xie ◽  
Wenxing He ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Redox Homeostasis ◽  
Soluble Sugars ◽  
Dry Weight ◽  
Limiting Factors ◽  
Salt Tolerant ◽  
Fresh Weight ◽  
Shoot Height ◽  
Wheat Genotypes

Salinity is one of the limiting factors of wheat production worldwide. A total of 334 internationally derived wheat genotypes were employed to identify new germplasm resources for salt tolerance breeding. Salt stress caused 39, 49, 58, 55, 21 and 39% reductions in shoot dry weight (SDW), root dry weight (RDW), shoot fresh weight (SFW), root fresh weight (RFW), shoot height (SH) and root length (RL) of wheat, respectively, compared with the control condition at the seedling stage. The wheat genotypes showed a wide genetic and tissue diversity for the determined characteristics in response to salt stress. Finally, 12 wheat genotypes were identified as salt-tolerant through a combination of one-factor (more emphasis on the biomass yield) and multifactor analysis. In general, greater accumulation of osmotic substances, efficient use of soluble sugars, lower Na+/K+ and a higher-efficiency antioxidative system contribute to better growth in the tolerant genotypes under salt stress. In other words, the tolerant genotypes are capable of maintaining stable osmotic potential and ion and redox homeostasis and providing more energy and materials for root growth. The identified genotypes with higher salt tolerance could be useful for developing new salt-tolerant wheat cultivars as well as in further studies to underline the genetic mechanisms of salt tolerance in wheat.

scholarly journals Identification and development of novel salt-responsive candidate gene based SSRs (cg-SSRs) and MIR gene based SSRs (mir-SSRs) in bread wheat (Triticum aestivum)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Geetika Mehta ◽  
Senthilkumar K. Muthusamy ◽  
G. P. Singh ◽  
Pradeep Sharma
Keyword(s):  
Genetic Diversity ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Candidate Gene ◽  
Stress Tolerance ◽  
Bread Wheat ◽  
Diversity Analysis ◽  
Salt Tolerant ◽  
Wheat Genotypes ◽  
Salt Tolerant Cultivars

AbstractSalt stress adversely affects the global wheat production and productivity. To improve salinity tolerance of crops, identification of robust molecular markers is highly imperative for development of salt-tolerant cultivars to mimic yield losses under saline conditions. In this study, we mined 171 salt-responsive genes (including 10 miRNAs) from bread wheat genome using the sequence information of functionally validated salt-responsive rice genes. Salt-stress, tissue and developmental stage-specific expression analysis of RNA-seq datasets revealed the constitutive as well as the inductive response of salt-responsive genes in different tissues of wheat. Fifty-four genotypes were phenotyped for salt stress tolerance. The stress tolerance index of the genotypes ranged from 0.30 to 3.18. In order to understand the genetic diversity, candidate gene based SSRs (cg-SSRs) and MIR gene based SSRs (miR-SSRs) were mined from 171 members of salt-responsive genes of wheat and validated among the contrasting panels of 54 tolerant as well as susceptible wheat genotypes. Among 53 SSR markers screened, 10 cg-SSRs and 8 miR-SSRs were found to be polymorphic. Polymorphic information content between the wheat genotypes ranged from 0.07 to 0.67, indicating the extant of wide genetic variation among the salt tolerant and susceptible genotypes at the DNA level. The genetic diversity analysis based on the allelic data grouped the wheat genotypes into three separate clusters of which single group encompassing most of the salt susceptible genotypes and two of them containing salt tolerance and moderately salt tolerance wheat genotypes were in congruence with penotypic data. Our study showed that both salt-responsive genes and miRNAs based SSRs were more diverse and can be effectively used for diversity analysis. This study reports the first extensive survey on genome-wide analysis, identification, development and validation of salt-responsive cg-SSRs and miR-SSRs in wheat. The information generated in the present study on genetic divergence among genotypes having a differential response to salt will help in the selection of suitable lines as parents for developing salt tolerant cultivars in wheat.

scholarly journals A NAC transcription factor OsNAC3 positively regulates ABA response and salt tolerance in rice

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Xiang Zhang ◽  
Yan Long ◽  
Xingxiang Chen ◽  
Baolei Zhang ◽  
Yafeng Xin ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Opposite Effect ◽  
Abiotic Stress Tolerance ◽  
Stress Sensitivity ◽  
Limiting Factors ◽  
Regulatory Factor ◽  
Root Cells ◽  
Qrt Pcr ◽  
Aba Response

Abstract Background NAC (NAM, ATAF and CUC) transcription factors (TFs) play vital roles in plant development and abiotic stress tolerance. Salt stress is one of the most limiting factors for rice growth and production. However, the mechanism underlying salt tolerance in rice is still poorly understood. Results In this study, we functionally characterized a rice NAC TF OsNAC3 for its involvement in ABA response and salt tolerance. ABA and NaCl treatment induced OsNAC3 expression in roots. Immunostaining showed that OsNAC3 was localized in all root cells. OsNAC3 knockout decreased rice plants’ sensitivity to ABA but increased salt stress sensitivity, while OsNAC3 overexpression showed an opposite effect. Loss of OsNAC3 also induced Na+ accumulation in the shoots. Furthermore, qRT-PCR and transcriptomic analysis were performed to identify the key OsNAC3 regulated genes related to ABA response and salt tolerance, such as OsHKT1;4, OsHKT1;5, OsLEA3–1, OsPM-1, OsPP2C68, and OsRAB-21. Conclusions This study shows that rice OsNAC3 is an important regulatory factor in ABA signal response and salt tolerance.

Application of SSR Technique for the Identification of Markers Linked to Salinity Tolerance in Rice

2013 ◽  
Vol 19 (2) ◽  
pp. 57-65
Author(s):  
MH Kabir ◽  
MM Islam ◽  
SN Begum ◽  
AC Manidas
Keyword(s):  
Salt Tolerance ◽  
Ssr Markers ◽  
Salinity Tolerance ◽  
Seedling Stage ◽  
Phenotypic Screening ◽  
Salt Tolerant ◽  
Marker Assisted Breeding ◽  
Hydroponic System ◽  
Rice Landrace ◽  
Salt Susceptible

A cross was made between high yielding salt susceptible BINA variety (Binadhan-5) with salt tolerant rice landrace (Harkuch) to identify salt tolerant rice lines. Thirty six F3 rice lines of Binadhan-5 x Harkuch were tested for salinity tolerance at the seedling stage in hydroponic system using nutrient solution. In F3 population, six lines were found as salt tolerant and 10 lines were moderately tolerant based on phenotypic screening at the seedling stage. Twelve SSR markers were used for parental survey and among them three polymorphic SSR markers viz., OSR34, RM443 and RM169 were selected to evaluate 26 F3 rice lines for salt tolerance. With respect to marker OSR34, 15 lines were identified as salt tolerant, 9 lines were susceptible and 2 lines were heterozygous. While RM443 identified 3 tolerant, 14 susceptible and 9 heterozygous rice lines. Eight tolerant, 11 susceptible and 7 heterozygous lines were identified with the marker RM169. Thus the tested markers could be efficiently used for tagging salt tolerant genes in marker-assisted breeding programme.DOI: http://dx.doi.org/10.3329/pa.v19i2.16929 Progress. Agric. 19(2): 57 - 65, 2008

Genetic Potential for Salt Tolerance During Germination in Lycopersicon Species

HortScience ◽  
1997 ◽  
Vol 32 (2) ◽  
pp. 296-300 ◽  
Author(s):  
M.R. Foolad ◽  
G.Y. Lin
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Genotypic Variation ◽  
Control Treatment ◽  
Molar Ratio ◽  
Wild Accession ◽  
Salt Tolerant ◽  
Plant Introductions ◽  
Genetic Potential ◽  
Stress Levels

Seed of 42 wild accessions (Plant Introductions) of Lycopersicon pimpinellifolium Jusl., 11 cultigens (cultivated accessions) of L. esculentum Mill., and three control genotypes [LA716 (a salt-tolerant wild accession of L. pennellii Corr.), PI 174263 (a salt-tolerant cultigen), and UCT5 (a salt-sensitive breeding line)] were evaluated for germination in either 0 mm (control) or 100 mm synthetic sea salt (SSS, Na+/Ca2+ molar ratio equal to 5). Germination time increased in response to salt-stress in all genotypes, however, genotypic variation was observed. One accession of L. pimpinellifolium, LA1578, germinated as rapidly as LA716, and both germinated more rapidly than any other genotype under salt-stress. Ten accessions of L. pimpinellifolium germinated more rapidly than PI 174263 and 35 accessions germinated more rapidly than UCT5 under salt-stress. The results indicate a strong genetic potential for salt tolerance during germination within L. pimpinellifolium. Across genotypes, germination under salt-stress was positively correlated (r = 0.62, P < 0.01) with germination in the control treatment. The stability of germination response at diverse salt-stress levels was determined by evaluating germination of a subset of wild, cultivated accessions and the three control genotypes at 75, 150, and 200 mm SSS. Seeds that germinated rapidly at 75 mm also germinated rapidly at 150 mm salt. A strong correlation (r = 0.90, P < 0.01) existed between the speed of germination at these two salt-stress levels. At 200 mm salt, most accessions (76%) did not reach 50% germination by 38 days, demonstrating limited genetic potential within Lycopersicon for salt tolerance during germination at this high salinity.

scholarly journals Evaluation of salinity tolerance indices in North African barley accessions at reproductive stage

2019 ◽  
Vol 55 (No. 2) ◽  
pp. 61-69 ◽  
Author(s):  
Dorsaf Allel ◽  
Anis BenAmar ◽  
Mounawer Badri ◽  
Chedly Abdelly
Keyword(s):  
Salt Tolerance ◽  
Grain Yield ◽  
Salinity Tolerance ◽  
Selection Criteria ◽  
Reproductive Stage ◽  
Shoot Biomass ◽  
Salt Tolerant ◽  
North African ◽  
Grain Number ◽  
Selection Indices

Soil salinity is one of the main factors limiting cereal productivity in worldwide agriculture. Exploitation of natural variation in local barley germplasm is an effective approach to overcome yield losses. Three gene pools of North African Hordeum vulgare L. grown in Tunisia, Algeria and Egypt were evaluated at the reproductive stage under control and saline conditions. Assessment of stress tolerance was monitored using morphological, yield-related traits and phenological parameters of reproductive organs showing significant genetic variation. High heritability and positive relationships were found suggesting that some traits associated with salt tolerance could be used as selection criteria. The phenotypic correlations revealed that vegetative traits including shoot biomass, tiller number and leaf number along with yield-related traits such as spike number, one spike dry weight, grain number/plant and grain number/spike were highly positively correlated with grain yield under saline conditions. Hence, these traits can be used as reliable selection criteria to improve barley grain yield. Keeping a higher shoot biomass and longer heading and maturity periods as well as privileged filling ability might contribute to higher grain production in barley and thus could be potential target traits in barley crop breeding toward improvement of salinity tolerance. Multiple selection indices revealed that salt tolerance trait index provided a better discrimination of barley landraces allowing selection of highly salt-tolerant and highly productive genotypes under severe salinity level. Effective evaluation of salt tolerance requires an integration of selection indices to successfully identify and characterize salt tolerant lines required for valuable exploitation in the management of salt-affected areas.  

scholarly journals Early Growth Stage Characterization and the Biochemical Responses for Salinity Stress in Tomato

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 712
Author(s):  
Md Sarowar Alam ◽  
Mark Tester ◽  
Gabriele Fiene ◽  
Magdi Ali Ahmed Mousa
Keyword(s):  
Salt Tolerance ◽  
Salinity Tolerance ◽  
Salinity Treatment ◽  
Salt Tolerant ◽  
Improvement Program ◽  
Biochemical Basis ◽  
Elevated Salinity ◽  
Tolerance Indices ◽  
Promising Source ◽  
Tomato Genotypes

Salinity is one of the most significant environmental stresses for sustainable crop production in major arable lands of the globe. Thus, we conducted experiments with 27 tomato genotypes to screen for salinity tolerance at seedling stage, which were treated with non-salinized (S1) control (18.2 mM NaCl) and salinized (S2) (200 mM NaCl) irrigation water. In all genotypes, the elevated salinity treatment contributed to a major depression in morphological and physiological characteristics; however, a smaller decrease was found in certain tolerant genotypes. Principal component analyses (PCA) and clustering with percentage reduction in growth parameters and different salt tolerance indices classified the tomato accessions into five key clusters. In particular, the tolerant genotypes were assembled into one cluster. The growth and tolerance indices PCA also showed the order of salt-tolerance of the studied genotypes, where Saniora was the most tolerant genotype and P.Guyu was the most susceptible genotype. To investigate the possible biochemical basis for salt stress tolerance, we further characterized six tomato genotypes with varying levels of salinity tolerance. A higher increase in proline content, and antioxidants activities were observed for the salt-tolerant genotypes in comparison to the susceptible genotypes. Salt-tolerant genotypes identified in this work herald a promising source in the tomato improvement program or for grafting as scions with improved salinity tolerance in tomato.

Short-term salt tolerance mechanisms in differentially salt tolerant tomato species

1999 ◽  
Vol 37 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Ana Santa-Cruz ◽  
Manuel Acosta ◽  
Ana Rus ◽  
Maria C. Bolarin
Keyword(s):  
Salt Tolerance ◽  
Salt Tolerant ◽  
Tolerance Mechanisms ◽  
Short Term ◽  
Tomato Species
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