scholarly journals High Density Single Nucleotide Polymorphism (SNP) Mapping and Quantitative Trait Loci (QTL) Analysis in a Biparental Spring Triticale Population Localized Major and Minor Effect Fusarium Head Blight Resistance and Associated Traits QTL

Genes ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 19 ◽  
Author(s):  
Raman Dhariwal ◽  
George Fedak ◽  
Yves Dion ◽  
Curtis Pozniak ◽  
André Laroche ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Qtl Analysis ◽  
Fusarium Head Blight Resistance ◽  
Blight Resistance ◽  
Minor Effect ◽  
Nucleotide Polymorphism ◽  
Head Blight ◽  
Single Nucleotide ◽  
Spring Triticale ◽  
Snp Mapping

scholarly journals A single-nucleotide-polymorphism-based genotyping assay for simultaneous detection of different carbendazim-resistant genotypes in the Fusarium graminearum species complex

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2609 ◽  
Author(s):  
Hao Zhang ◽  
Balázs Brankovics ◽  
Theo A.J. van der Lee ◽  
Cees Waalwijk ◽  
Anne A.D. van Diepeningen ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Fusarium Graminearum ◽  
Species Complex ◽  
Simultaneous Detection ◽  
Point Mutations ◽  
Nucleotide Polymorphism ◽  
Head Blight ◽  
Single Nucleotide ◽  
Allele Specific ◽  
Resistant Genotypes

The occurrence resistance to methyl benzimidazole carbamates (MBC)-fungicides in the Fusarium graminearum species complex (FGSC) is becoming a serious problem in the control of Fusarium head blight in China. The resistance is caused by point mutations in the β2-tubulingene. So far, five resistant genotypes (F167Y, E198Q, E198L, E198K and F200Y) have been reported in the field. To establish a high-throughput method for rapid detection of all the five mutations simultaneously, an efficient single-nucleotide-polymorphism-based genotyping method was developed based on the Luminex xMAP system. One pair of amplification primers and five allele specific primer extension probes were designed and optimized to specially distinguish the different genotypes within one single reaction. This method has good extensibility and can be combined with previous reported probes to form a highly integrated tool for species, trichothecene chemotype and MBC resistance detection. Using this method, carbendazim resistant FGSC isolates from Jiangsu, Anhui and Sichuan Province in China were identified. High and moderate frequencies of resistance were observed in Jiangsu and Anhui Province, respectively. Carbendazim resistance in F. asiaticum is only observed in the 3ADON genotype. Overall, our method proved to be useful for early detection of MBC resistance in the field and the result aids in the choice of fungicide type.

Genetic Map and QTL Analysis of Agronomic Traits in a Diploid Potato Population using Single Nucleotide Polymorphism Markers

Crop Science ◽  
2015 ◽  
Vol 55 (6) ◽  
pp. 2566-2579 ◽  
Author(s):  
Norma C. Manrique-Carpintero ◽  
Joseph J. Coombs ◽  
Yuehua Cui ◽  
Richard E. Veilleux ◽  
C. Robin Buell ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Genetic Map ◽  
Qtl Analysis ◽  
Agronomic Traits ◽  
Diploid Potato ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide
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