scholarly journals Identification of Candidate Genes for Seed Glucosinolate Content Using Association Mapping in Brassica napus L.

Genes ◽  
2015 ◽  
Vol 6 (4) ◽  
pp. 1215-1229 ◽  
Author(s):  
Cun-Min Qu ◽  
Shi-Meng Li ◽  
Xiu-Jian Duan ◽  
Jin-Hua Fan ◽  
Le-Dong Jia ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Association Mapping ◽  
Candidate Genes ◽  
Glucosinolate Content ◽  
Brassica Napus L

scholarly journals Integrating GWAS, linkage mapping and gene expression analyses reveals the genetic control of growth period traits in rapeseed (Brassica napus L.)

2020 ◽  
Author(s):  
tengyue wang ◽  
Lijuan Wei ◽  
Jia wang ◽  
Ling Xie ◽  
Yangyang Li ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Association Mapping ◽  
Candidate Genes ◽  
Linkage Mapping ◽  
Growth Period ◽  
Phenotypic Traits ◽  
Multiple Traits ◽  
Brassica Napus L ◽  
Genome Wide ◽  
Ril Population

Abstract Background: Brassica napus is one of the most important oilseed crops, and also an important biofuel plant due to its low air pollution and renewability. Growth period are important traits that affect yield and are crucial for its adaptation to different environments in B. napus.Results: To elucidate the genetic basis of growth period traits, genome-wide association analysis (GWAS) and linkage mapping were employed to detect the quantitative trait loci (QTL) for days to initial flowering (DIF), days to final flowering (DFF), days from initial flowering to final flowering (FP), days from final flowering to maturity (MT), and whole growth period (GP). A total of 146 SNPs were identified by association mapping, and 83 QTLs were identified by linkage mapping using the RIL population. Among these QTLs, 19 were pleiotropic SNPs related to multiple traits, and six (q18DFF.A03-2, q18MT.A03-2, q17DFF.A05-1, q18FP.C04, q17DIF.C05 and q17GP.C09) were consistently detected using both mapping methods. Additionally, we performed RNA sequencing to analyze the differential expression of gene (DEG) transcripts between early- and late-flowering lines selected from the RIL population, and the DEGs were integrated with association mapping and linkage analysis to confirm their roles in the growth period. Consequently, twelve candidate genes associated with growth period traits were identified in B. napus. Among these genes, seven have polymorphic sites in the coding sequence and the upstream 2-kb sequence based on the resequencing data. The haplotype BnaSOC1.A05-Haplb and BnaLNK2.C06-Hapla showed more favorable phenotypic traits. Conclusions: The candidate genes identified in this study will contribute to our genetic understanding of growth period traits and can be used as targets for target mutations or marker assisted breeding for rapeseed adapted to different environments.

scholarly journals Integrating GWAS , linkage mapping and gene expression analyses reveals the genetic control of growth period traits in rapeseed (Brassica napus L.)

2020 ◽  
Author(s):  
tengyue wang ◽  
Lijuan Wei ◽  
Jia wang ◽  
Ling Xie ◽  
Yangyang Li ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Association Mapping ◽  
Candidate Genes ◽  
Linkage Mapping ◽  
Growth Period ◽  
Phenotypic Traits ◽  
Multiple Traits ◽  
Brassica Napus L ◽  
Genome Wide ◽  
Ril Population

Abstract Background: Brassica napus is one of the most important oilseed crops, and also an important biofuel plant due to its low air pollution and renewability. Growth period are important traits that affect yield and are crucial for its adaptation to different environments in B. napus . Results: To elucidate the genetic basis of growth period traits, genome-wide association analysis (GWAS) and linkage mapping were employed to detect the quantitative trait loci (QTL) for days to initial flowering (DIF), days to final flowering (DFF), days from initial flowering to final flowering (FP), days from final flowering to maturity (MT), and whole growth period (GP). A total of 146 SNPs were identified by association mapping, and 83 QTLs were identified by linkage mapping using the RIL population. Among these QTLs, 19 were pleiotropic SNPs related to multiple traits, and six (q18DFF.A03-2, q18MT.A03-2, q17DFF.A05-1, q18FP.C04, q17DIF.C05 and q17GP.C09) were consistently detected using both mapping methods. Additionally, we performed RNA sequencing to analyze the differential expression of gene (DEG) transcripts between early- and late-flowering lines selected from the RIL population, and the DEGs were integrated with association mapping and linkage analysis to confirm their roles in the growth period. Consequently, twelve candidate genes associated with growth period traits were identified in B. napus . Among these genes, seven have polymorphic sites in the coding sequence and the upstream 2-kb sequence based on the resequencing data. The haplotype BnaSOC1.A05-Haplb and BnaLNK2.C06-Hapla showed more favorable phenotypic traits. Conclusions: The candidate genes identified in this study will contribute to our genetic understanding of growth period traits and can be used as targets for target mutations or marker assisted breeding for rapeseed adapted to different environments.

NUTRITIONAL VALUE OF LOW GLUCOSINOLATE RAPESEED MEAL FOR SWINE

1975 ◽  
Vol 55 (1) ◽  
pp. 61-70 ◽  
Author(s):  
J. M. BELL
Keyword(s):  
Brassica Napus ◽  
Soybean Meal ◽  
Nutritional Value ◽  
Control Diet ◽  
Rapeseed Meal ◽  
Glucosinolate Content ◽  
Protein Digestion ◽  
Brassica Napus L ◽  
Ad Libitum

Five swine experiments were conducted to evaluate rapeseed meal (RSM) of low glucosinolate content (Brassica napus L. cv. Bronowski). Two experiments involved 0, 25, 50, 75 and 100% substitution of either Bronowski meal or regular (B. campestris) meal for soybean meal or fishmeal used in the control diet. One experiment compared ad libitum-fed and partially restricted pigs. Another experiment involved digestibility studies, and the final one involved methionine and lysine supplementation. As the dietary levels of either Bronowski or regular RSM increased in the ration, protein digestion coefficients decreased from 79 and 80% to 76 and 78%, respectively, and energy coefficients decreased from 82% to 79 and 78%, respectively. The protein and energy digestibility coefficients for Bronowski RSM were estimated to be 68 and 59%; for regular RSM, 65 and 54%. With barley–wheat–RSM diets, pigs responded to 0.1% methionine, but not to lysine (P > 0.05). Pigs fed ad libitum consumed more Bronowski than regular RSM diet and performed as well as pigs fed soybean meal diets.

Variation in the glucosinolate content of vegetative tissues of Chinese lines of Brassica napus L

1999 ◽  
Vol 134 (1) ◽  
pp. 131-136 ◽  
Author(s):  
YUNCHANG LI ◽  
GUY KIDDLE ◽  
RICHARD BENNETT ◽  
KEVIN DOUGHTY ◽  
ROGER WALLSGROVE
Keyword(s):  
Brassica Napus ◽  
Glucosinolate Content ◽  
Brassica Napus L ◽  
Vegetative Tissues
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