scholarly journals Molecular Breeding for Improving Productivity of Oryza sativa L. cv. Pusa 44 under Reproductive Stage Drought Stress through Introgression of a Major QTL, qDTY12.1

Genes ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 967
Author(s):  
Kyaw Swar Oo ◽  
Subbaiyan Gopala Krishnan ◽  
Kunnummal Kurungara Vinod ◽  
Gaurav Dhawan ◽  
Priyanka Dwivedi ◽  
...  
Keyword(s):  
Drought Stress ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
Phenotypic Selection ◽  
Rice Production ◽  
Reproductive Stage ◽  
High Yield ◽  
Recurrent Parent ◽  
Global Food Security ◽  
Successful Recovery

Increasing rice production is quintessential to the task of sustaining global food security, as a majority of the global population is dependent on rice as its staple dietary cereal. Among the various constraints affecting rice production, reproductive stage drought stress (RSDS) is a major challenge, due to its direct impact on grain yield. Several quantitative trait loci (QTLs) conferring RSDS tolerance have been identified in rice, and qDTY12.1 is one of the major QTLs reported. We report the successful introgression of qDTY12.1 into Pusa 44, a drought sensitive mega rice variety of the northwestern Indian plains. Marker-assisted backcross breeding (MABB) was adopted to transfer qDTY12.1 into Pusa 44 in three backcrosses followed by four generations of pedigree selection, leading to development of improved near isogenic lines (NILs). Having a recurrent parent genome (RPG) recovery ranging from 94.7–98.7%, the improved NILs performed 6.5 times better than Pusa 44 under RSDS, coupled with high yield under normal irrigated conditions. The MABB program has been modified so as to defer background selection until BC3F4 to accelerate generational advancements. Deploying phenotypic selection alone in the early backcross generations could help in the successful recovery of RPG. In addition, the grain quality could be recovered in the improved NILs, leading to superior selections. Owing to their improved adaptation to drought, the release of improved NILs for regions prone to intermittent drought can help enhance rice productivity and production.

scholarly journals Identification of fgr gene and preliminary evaluation for agronomic traits of pandan sticky rice variety (Oryza sativa L.)

2019 ◽  
Vol 02 ◽  
pp. 39-48
Author(s):  
Toan D. Pham
Keyword(s):  
Oryza Sativa ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
Rice Production ◽  
Molecular Techniques ◽  
High Yield ◽  
Preliminary Evaluation ◽  
Rice Varieties ◽  
Fgr Gene

Rice (Oryza sativa L.) is the most important food crop in Vietnam particularly in the Mekong Delta. Screening of good quality and high yield rice varieties are needed for rice production in Vietnam. The purpose of this study was to use molecular techniques to identify fgr gene and to evaluate preliminarily agronomic traits of pandan sticky rice variety. The results showed that this variety contained fgr gene. The fragrant allele was amplified by ESP - IFAP primers with a product of 255 bp in size. Similarly, evaluation of agronomic traits showed that the pandan sticky rice variety displayed many desirable characteristics such as plant height of 108 cm, panicle length of 25.6 cm, seed/panicle 135, 100-grain weight 2.07 g, pandan smell, level 2 of alkali digestion, gel consistency 93 mm. These results were useful information and could be applied for improving and providing pandan sticky rice variety for rice production.

scholarly journals Introgression of qDTY1.1 Governing Reproductive Stage Drought Tolerance Into an Elite Rice Variety ‘Pusa Basmati 1’ Through Marker Assisted Backcross Breeding

2020 ◽  
Author(s):  
Gaurav Dhawan ◽  
Aruna Kumar ◽  
Priyanka Dwivedi ◽  
Subbaiyan Gopala Krishnan ◽  
Madan Pal ◽  
...  
Keyword(s):  
Drought Stress ◽  
Rice Variety ◽  
Reproductive Stage ◽  
Basmati Rice ◽  
Yield Potential ◽  
High Yield ◽  
Yield Reduction ◽  
Backcross Breeding ◽  
Selection For ◽  
Marker Assisted Backcross Breeding

Drought stress at the reproductive stage in rice is one of the most important cause for yield reduction, affecting both productivity and quality. All Basmati rice varieties, including the popular cultivar ‘Pusa Basmati 1 (PB1)’ is highly sensitive to reproductive stage drought stress (RSDS). We report for the first time, improvement of a Basmati cultivar for RSDS tolerance, with the introgression of a major quantitative trait locus (QTL), ‘qDTY1.1’ into PB1. The QTL donor was sourced from an aus variety, Nagina 22 (N22). A QTL linked microsatellite (SSR) marker ‘RM 431’ was employed for foreground selection for qDTY1.1 in the marker assisted backcross breeding process. A set of 113 SSR markers polymorphic between N22 and PB1 were utilized for background selection to ensure higher genome recovery. After three backcrosses followed by five generations of selfing, eighteen near isogenic lines (NILs) were developed, through combinatory selection for agro-morphological, grain and cooking superiority traits. The NILs were evaluated for three consecutive Kharif seasons, 2017, 2018 and 2019 under well-watered and drought stress conditions. RSDS tolerance and yield stability indicated that NIL3, NIL5, NIL6, NIL7, NIL12, NIL15 and NIL17 were best in terms of overall agronomic and grain quality under RSDS. Additionally, NILs exhibited high yield potential under normal condition as well. The RSDS tolerant Basmati NILs with high resilience to water stress, is a valuable resource for sustaining Basmati rice production under water limiting production environments.

scholarly journals Drought Tolerant near Isogenic Lines (NILs) of Pusa 44 Developed through Marker Assisted Introgression of qDTY2.1 and qDTY3.1 Enhances Yield under Reproductive Stage Drought Stress

Agriculture ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 64
Author(s):  
Priyanka Dwivedi ◽  
Naleeni Ramawat ◽  
Gaurav Dhawan ◽  
Subbaiyan Gopala Krishnan ◽  
Kunnummal Kurungara Vinod ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Grain Quality ◽  
Reproductive Stage ◽  
Recurrent Parent ◽  
Near Isogenic Lines ◽  
Improvement Project ◽  
Isogenic Lines ◽  
Drought Tolerant ◽  
Rice Improvement

Reproductive stage drought stress (RSDS) is detrimental for rice, which affects its productivity as well as grain quality. In the present study, we introgressed two major quantitative trait loci (QTLs), namely, qDTY2.1 and qDTY3.1, governing RSDS tolerance in a popular high yielding non-aromatic rice cultivar, Pusa 44, through marker-assisted backcross breeding (MABB). Pusa 44 is highly sensitive to RSDS, which restricts its cultivation across drought-prone environments. Foreground selection was carried out using markers, RM520 for qDTY3.1 and RM 521 for qDTY2.1. Background selection was achieved with 97 polymorphic SSR markers in tandem with phenotypic selection to achieve faster recurrent parent genome (RPG) recovery. Three successive backcrosses followed by three selfings aided RPG recoveries of 98.6% to 99.4% among 31 near isogenic lines (NILs). Fourteen NILs were found to be significantly superior in yield and grain quality under RSDS with higher drought tolerance efficiency (DTE) than Pusa 44. Among these, the evaluation of two promising NILs in the multilocational trial during Kharif 2019 showed that they were significantly superior to Pusa 44 under reproductive stage drought stress, while performing on par with Pusa 44 under normal irrigated conditions. These di-QTL pyramided drought-tolerant NILs are in the final stages of testing the All India Coordinated Rice Improvement Project varietal trials for cultivar release. Alternately, the elite drought-tolerant Pusa 44 NILs will serve as an invaluable source of drought tolerance in rice improvement.

scholarly journals Rice cold tolerance at the reproductive stage in a controlled environment

2006 ◽  
Vol 63 (3) ◽  
pp. 255-261 ◽  
Author(s):  
Renata Pereira da Cruz ◽  
Sandra Cristina Kothe Milach ◽  
Luiz Carlos Federizzi
Keyword(s):  
Cold Tolerance ◽  
Oryza Sativa L ◽  
Variable Temperature ◽  
Reproductive Stage ◽  
Spikelet Fertility ◽  
High Yield ◽  
Cold Temperatures ◽  
Cold Tolerant ◽  
Rice Genotypes ◽  
Highly Correlated

Cold tolerance of rice (Oryza sativa L.) during the reproductive stage is important to guarantee high yield under low temperature environments. Field selection, however, does not allow identification of adequate tolerance sources and limits selection of segregating lines due to variable temperature. The objective of this study was to devise methods for distinguishing rice genotypes as to their cold tolerance at the reproductive stage when evaluated under controlled temperature. The effect of cold temperatures was investigated in six rice genotypes at 17°C for varying length of time (three, five, seven and ten days) at two reproductive stages (microsporogenesis and anthesis). Cold tolerance was measured as the percentage of reduction in panicle exsertion and in spikelet fertility. Evaluating cold tolerance through the reduction in panicle exsertion did not allow for the distinction between cold tolerant from cold sensitive genotypes and, when the reduction in spikelet fertility was considered, a minimum of seven days was required to differentiate the genotypes for cold tolerance. Genotypes were more sensitive to cold at anthesis than at microsporogenesis and, as these stages were highly correlated, cold screening could be performed at anthesis only, since it is easier to determine. Rice cold tolerance at the reproductive stage may be characterized by the reduction in spikelet fertility due to cold temperature (17°C) applied for seven days at anthesis.

scholarly journals Marker assisted introgression of bacterial blight resistant gene into submergence tolerance rice variety BRRI dhan52

2017 ◽  
Vol 42 (3) ◽  
pp. 403-411 ◽  
Author(s):  
ME Kabir ◽  
KM Iftekharuddaula ◽  
MAI Khan ◽  
MAK Mian ◽  
NA Ivy
Keyword(s):  
Resistance Genes ◽  
Bacterial Blight ◽  
Flash Flood ◽  
Rice Variety ◽  
High Yield ◽  
Recurrent Parent ◽  
Breeding Lines ◽  
Resistant Gene ◽  
Rainfed Lowland ◽  
Elite Breeding Lines

BRRI dhan52 is a uniquire submergence tolerant rice variety containing prominant genetic background of BR11, a mega rainfed lowland rice (RLR) variety of Bangladesh, but is susceptible to bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo). The variety is considerably popular in the southern part of Bangladesh due to its high yield in flash flood condition and comparatvely medium slender grain. Molecular markers linked to BB resistance genes (Xa genes) and submergence QTL (SUB1) were utilized in a marker-aided selection program to develop elite breeding lines with broad-spectrum resistance to bacterial blight. Sequence tagged site (STS) and simple sequence repeat (SSR) markers were essentially used to detect the genes for BB and submergence as well. In backcross generation, markers closely linked to Xa21 and SUB1 QTL were used to select desirable plants possessing these resistance genes (foreground selection) and microsatellite markers polymorphic between donor and recurrent parent were also used to select plants that have maximum contribution from the recurrent parent genome (background selection). In BC1F1 generation, three best plants consiquently were selected from previously selected ten double heterozygous (Xa21 and SUB1 QTL) plants. The percentage of recipient genome recovery in the best plant 1, 2 and 3 were 78.7%, 75.83% and 75.4%, respectively. Eventually this work illustrates the successful application of marker-assisted breeding for introgression of bacterial blight resistant gene into a rice variety of Bangladesh.Bangladesh J. Agril. Res. 42(3): 403-411, September 2017

scholarly journals Heterosis-associated genes confer high yield in super hybrid rice

2020 ◽  
Vol 133 (12) ◽  
pp. 3287-3297
Author(s):  
Tianzi Lin ◽  
Cong Zhou ◽  
Gaoming Chen ◽  
Jun Yu ◽  
Wei Wu ◽  
...  
Keyword(s):  
Large Scale ◽  
Field Experiments ◽  
Hybrid Rice ◽  
Rice Variety ◽  
Female Parent ◽  
High Yield ◽  
Recurrent Parent ◽  
Sequencing Analysis ◽  
Substitution Lines ◽  
Chromosome Segment Substitution Lines

Abstract Key message Heterosis QTLs, including qSS7 and qHD8, with dominance effects were identified through GBS and large-scale phenotyping of CSSLs and hybrid F1 populations in a paddy field. Abstract Heterosis has contributed immensely to agricultural production, but its genetic basis is unclear. We evaluated dominance effects by creating two hybrid populations: a B-homo set with a homozygous background and heterozygous chromosomal segments and a B-heter set with a heterozygous background and homozygous segments. This was achieved by crossing a set of 156 backcrossed-derived chromosome segment substitution lines (CSSLs) with their recurrent parent (9311), the male parent of the first super-high-yield hybrid Liangyoupei9 (LYP9), and with the female parent (PA64s) of the hybrid. The CSSLs were subjected to a genotyping-by-sequencing analysis to develop a genetic map of segments introduced from the PA64s. We evaluated the heterotic effects on eight yield-related traits in the hybrid variety and F1 populations in large-scale field experiments over 2 years. Using a linkage map consisting of high-density SNPs, we identified heterosis-associated genes in LYP9. Five candidate genes contributed to the high yield of LYP9, with qSS7 and qHD8 repeatedly detected in both B-hybrid populations. The heterozygous segments harboring qSS7 and qHD8 showed dominance effects that contributed to the heterosis of yield components in the hybrid rice variety Liangyoupei9.

scholarly journals Association Mapping of Yield and Yield-related Traits Under Reproductive Stage Drought Stress in Rice (Oryza sativa L.)

Rice ◽  
2017 ◽  
Vol 10 (1) ◽  
Author(s):  
B. P. Mallikarjuna Swamy ◽  
Noraziyah Abd Aziz Shamsudin ◽  
Site Noorzuraini Abd Rahman ◽  
Ramil Mauleon ◽  
Wickneswari Ratnam ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Drought Stress ◽  
Association Mapping ◽  
Oryza Sativa L ◽  
Reproductive Stage

scholarly journals Introgression of qDTY1.1 Governing Reproductive Stage Drought Tolerance into an Elite Basmati Rice Variety “Pusa Basmati 1” through Marker Assisted Backcross Breeding

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 202
Author(s):  
Gaurav Dhawan ◽  
Aruna Kumar ◽  
Priyanka Dwivedi ◽  
Subbaiyan Gopala Krishnan ◽  
Madan Pal ◽  
...  
Keyword(s):  
Drought Stress ◽  
Reproductive Stage ◽  
Chromosome 1 ◽  
Basmati Rice ◽  
Yield Potential ◽  
Yield Reduction ◽  
Recurrent Parent ◽  
Backcross Breeding ◽  
Selection For ◽  
Marker Assisted Backcross Breeding

Drought stress at the reproductive stage in rice is one of the most important cause for yield reduction, affecting both productivity and quality. All Basmati rice varieties, including the popular cultivar “Pusa Basmati 1 (PB1)” is highly sensitive to reproductive stage drought stress (RSDS). We report for the first time, improvement of a Basmati cultivar for RSDS tolerance, with the introgression of a major quantitative trait locus (QTL), “qDTY1.1” into PB1. The QTL was sourced from an aus variety, Nagina 22 (N22). A microsatellite (simple sequence repeat (SSR)) marker “RM 431” located at telomeric end (38.89 mb) of chromosome 1, and located within a 1.04 mb QTL region was employed for foreground selection for qDTY1.1 in the marker assisted backcross breeding process. A set of 113 SSR markers polymorphic between N22 and PB1 were utilized for background selection to ensure higher recurrent parent genome recovery. After three backcrosses followed by five generations of selfing, eighteen near isogenic lines (NILs) were developed, through combinatory selection for agro-morphological, grain and cooking quality traits. The NILs were evaluated for three consecutive Kharif seasons, 2017, 2018 and 2019 under well-watered and drought stress conditions. RSDS tolerance and yield stability indicated that P1882-12-111-3, P1882-12-111-5, P1882-12-111-6, P1882-12-111-7, P1882-12-111-12, P1882-12-111-15 and P1882-12-111-17 were best in terms of overall agronomic and grain quality under RSDS. Additionally, NILs exhibited high yield potential under normal condition as well. The RSDS tolerant Basmati NILs with high resilience to water stress, is a valuable resource for sustaining Basmati rice production under water limiting production environments.

scholarly journals Differential Expression Proteins Contribute to Race-Specific Resistant Ability in Rice (Oryza sativa L.)

Plants ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 29 ◽  
Author(s):  
Shiwei Ma ◽  
Shoukai Lin ◽  
Menglin Wang ◽  
Yang Zou ◽  
Huan Tao ◽  
...  
Keyword(s):  
Magnaporthe Grisea ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
Differentially Expressed Proteins ◽  
Near Isogenic Lines ◽  
Rice Varieties ◽  
Global Food Security ◽  
Rice Yields ◽  
And Function ◽  
Global Food

Rice blast, caused by the fungus, Magnaporthe grisea (M. grisea), lead to the decrease of rice yields widely and destructively, threatening global food security. Although many resistant genes had been isolated and identified in various rice varieties, it is still not enough to clearly understand the mechanism of race-specific resistant ability in rice, especially on the protein level. In this research, proteomic methods were employed to analyze the differentially expressed proteins (DEPs) in susceptible rice variety CO39 and its two near isogenic lines (NILs), CN-4a and CN-4b, in response to the infection of two isolates with different pathogenicity, GUY11 and 81278ZB15. A total of 50 DEPs with more than 1.5-fold reproducible change were identified. At 24 and 48 hpi of GUY11, 32 and 16 proteins in CN-4b were up-regulated, among which 16 and five were paralleled with the expression of their corresponding RNAs. Moreover, 13 of 50 DEPs were reported to be induced by M. grisea in previous publications. Considering the phenotypes of the three tested rice varieties, we found that 21 and 23 up-regulated proteins were responsible for the rice resistant ability to the two different blast isolates, 81278ZB15 and GUY11, respectively. Two distinct branches corresponding to GUY11 and 81278ZB15 were observed in the expression and function of the module cluster of DEPs, illuminating that the DEPs could be responsible for race-specific resistant ability in rice. In other words, DEPs in rice are involved in different patterns and functional modules’ response to different pathogenic race infection, inducing race-specific resistant ability in rice.

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