scholarly journals Association Study of SLC6A4 (5-HTTLPR) Polymorphism and Its Promoter Methylation with Rehabilitation Outcome in Patients with Subacute Stroke

Genes ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 579
Author(s):  
Massimo Santoro ◽  
Mariacristina Siotto ◽  
Marco Germanotta ◽  
Alessia Mastrorosa ◽  
Dionysia Papadopoulou ◽  
...  
Keyword(s):  
Promoter Methylation ◽  
Cpg Islands ◽  
Biological Marker ◽  
Favorable Outcome ◽  
Stroke Recovery ◽  
Non Invasive ◽  
Cpg Sites ◽  
Subacute Stroke ◽  
Significant Difference ◽  
Before And After

Recently it has been suggested that serotonin transporter (SLC6A4) and its 5HTTLPR polymorphism could be involved in post stroke recovery. Here, we characterized the methylation profile of two different CpG islands within the SLC6A4 promoter region in the whole blood of 50 patients with subacute stroke before and after a six-week rehabilitation treatment. These patients were genotyped for 5HTTLPR polymorphism identifying patients on the basis of short (S) and L (L) alleles: 17 patients LL, 22 patients LS and 11 patients SS. At baseline, all CpG sites for both CpG islands displayed a heterogeneous methylation percentage that were not influenced by the different genotypes. After rehabilitation, we found a significant variation in the methylation levels (increase/decrease) in the specific CpG sites of both CpG islands. The statistical analysis showed a significant relationship between the LL, LS and SS alleles and the outcome of the rehabilitation intervention (χ2 (2,50) = 6.395, p = 0.041). Specifically, we found a significant difference between patients with or without a favorable outcome in the LL (11.1% with a favorable outcome) and in the SS (54.4% with a favorable outcome) groups. Our data suggest that 5-HTTLPR polymorphisms and SLC6A4 promoter methylation may be employed as a non-invasive biological marker of recovery in patients with stroke undergoing rehabilitation.

Abstract 36: FCGR2A Promoter Methylation and Risks for IVIG Unresponsiveness in Kawasaki Disease

Circulation ◽  
2015 ◽  
Vol 131 (suppl_2) ◽  
Author(s):  
Ho-Chang Kuo ◽  
Kai-Sheng Hsieh ◽  
Wei-Chiao Chang ◽  
Keyword(s):  
Kawasaki Disease ◽  
Promoter Methylation ◽  
Systemic Vasculitis ◽  
Methylation Status ◽  
Cpg Islands ◽  
Gene Promoter ◽  
Ivig Therapy ◽  
Ivig Treatment ◽  
Fc Fragment ◽  
Cpg Sites

Kawasaki disease (KD) is characterized by pediatric systemic vasculitis of an unknown cause and the Fc Fragment of IgG, Low Affinity IIa, Receptor ( FCGR2A ) gene was reported to involve in increasing susceptibility of KD. Because DNA methylation is one of the epigenetic mechanisms that control gene expression, we hypothesized that methylation status of CpG islands in FCGR2A promoter predisposes an individual to Kawasaki disease. We recruited 36 KD patients and 24 healthy subjects with informed consents. And eleven potential methylation loci within the targeted promoter region (chr1:161474603-161475102) of Fc Fragment of IgG, Low Affinity IIa, Receptor were selected for investigation. Methylation at the CpG sites G, H and J displayed a strongly associations with KD, whereas CpG sites B,C,E,F,H,J and K were found to be correlated with non-responsive to IVIG treatment. In addition, CpG sites G, J and K were predicted as the significant transcription factor binding site for NF-kB, Myc-Max and SP2 respectively. Our study reports a significant association between the promoter methylation of FCGR2A , susceptibility of Kawasaki disease and therapeutic outcomes of IVIG treatment. The methylation levels of CpG sites of FCGR2A gene promoter may be an important marker for optimizing IVIG therapy.

scholarly journals Promoter Hypomethylation of miR-124 Gene Is Associated With Major Depressive Disorder

2021 ◽  
Vol 14 ◽  
Author(s):  
Duan Zeng ◽  
Shen He ◽  
Nan Zhao ◽  
Manji Hu ◽  
Jie Gao ◽  
...  
Keyword(s):  
Major Depressive Disorder ◽  
Depressive Disorder ◽  
Antidepressant Treatment ◽  
Cpg Islands ◽  
Healthy Controls ◽  
Lasso Regression ◽  
Bonferroni Correction ◽  
Major Depressive ◽  
Cpg Sites ◽  
Significant Difference

Based on our previous studies and other evidence, miR-124 is an important biomarker and therapeutic target for major depressive disorder (MDD). The aim of this study was to clarify the role of miR-124 methylation in MDD and antidepressant effects from the perspective of epigenetics. MethylTarget™ was used to detect methylation levels of the three miR-124 precursor genes (MIR124-1, MIR124-2, and MIR124-3) in 33 pre- and post-treatment MDD patients and 33 healthy controls. A total of 11 cytosine-phosphate-guanine (CpG) islands in the three miR-124 precursor genes, including 222 CpG sites, were detected. All CpG islands were hypomethylated in MDD patients when compared to healthy controls and seven CpG regions were still identified with a statistically significant difference after Bonferroni correction. In addition, 137 of 222 CpG sites were found a statistical difference between MDD patients and controls, and 40 CpG sites were still statistically significant after Bonferroni correction. After performing the LASSO regression model, seven biomarkers with differential methylation among 40 CpG sites were identified. Mean methylation score was lower in MDD patients (z = −5.84, p = 5.16E-9). The AUC value reached 0.917 (95% CI: 0.854–0.981) to discriminate MDD and controls. No changes in methylation of the three miR-124 precursor genes were found in MDD patients following antidepressant treatment. The methylation of miR-124 could be a promising diagnostic biomarker for MDD.

scholarly journals Physical therapy combined with a laxative fruit drink for treatment of chagasic megacolon

2011 ◽  
Vol 48 (1) ◽  
pp. 52-57 ◽  
Author(s):  
Egle Pereira Leão ◽  
Carlos José Martins Pena ◽  
Silvana Marques de Araújo ◽  
Mônica Lúcia Gomes
Keyword(s):  
Physical Therapy ◽  
Low Cost ◽  
Chagasic Megacolon ◽  
Non Invasive ◽  
Daily Ingestion ◽  
Significant Difference ◽  
Before And After ◽  
Bowel Movements ◽  
Fruit Drink ◽  
Therapy Interventions

CONTEXT: The treatment of Chagas' disease colopathy is limited to clinical management in the initial of the process, and for patients for whom surgery is not indicated or is not possible, anti-constipation diets are used, along with judicious administration of laxatives and enemas. OBJECTIVE: To evaluate over time the effects of physical-therapy interventions combined with daily ingestion of a laxative fruit drink in the treatment of chagasic megacolon. METHOD: In a quantitative, prospective, and comparative study, 12 patients of both sexes and with a mean age of 67 ± 12 years were clinically evaluated to receive 12 sessions of physical therapy twice a week, along with fruit drink, and were evaluated for intestinal constipation before and after treatment. RESULTS: A significant difference (P<0.0022) was observed in the constipation scores before and after 6 weeks of intervention in 91.7% of the patients, and in 72.7% after 12 months, with reduction of laxative medications, softer stools, and increased number of bowel movements. With respect to gender, age, and whether or not the patient had received surgical treatment, there was no significant difference (P>0.05). CONCLUSION: The proposed protocol is easy to implement, safe, non-invasive, and low-cost, with the potential to be deployed in health care by providing benefits independent of gender, age, or whether the participant has undergone surgery, improving the condition of patients with chagasic megacolon.

The Impact of Artificial Tear Viscosity on the Results of Optical Coherence Tomography

2020 ◽  
Author(s):  
Elisa Hannemann ◽  
Carl Erb
Keyword(s):  
Optical Coherence Tomography ◽  
Hyaluronic Acid ◽  
Tear Film ◽  
Optical Coherence ◽  
Non Invasive ◽  
Significant Difference ◽  
Measurement Results ◽  
Before And After ◽  
Break Up ◽  
The Impact

Abstract Background In earlier studies, an irregular tear film could be responsible for artifacts in imaging procedures in ophthalmology. As a result, this study will investigate the influence of hyaluronic acid-containing tear substitutes of different viscosities on the measurement results of optical coherence tomography and on the non-invasive tear film break-up time. Methods The study included three sessions in which three hyaluronic acid tear substitutes of different viscosities (Hylo-Vision 0.1%, 0.2%, 0.3%, OmniVision GmbH, Puchheim, Germany) were applied to each subject. In 20 healthy volunteers, the non-invasive tear film break-up time was measured before and after the application of the tear substitutes using Keratograph 5M (Oculus GmbH, Wetzlar, Germany) and optical coherence tomography using 3D OCT-2000 (Topcon, Hamburg, Germany). Results The median age of the volunteers was 28.5 years. 11 women (55%) and 9 men (45%) were examined. The non-invasive tear film break-up time improved significantly (p = 0.027) after the application of 0.3% hyaluronic acid. In the study, no significant difference was found in all OCT parameters used before and after application of the different viscous hyaluronic acid-containing tear substitutes. Conclusion In this study, no influence of tear substitutes containing different viscosities of hyaluronic acid on the measurement results of optical coherence tomography could be determined. Therefore, 0.1%, 0.2% and 0.3% hyaluronic acid can be applied to the patient to improve the corneal surface before the examination with optical coherence tomography, without influencing the measurement results of optical coherence tomography.

scholarly journals Acupuncture with Rehabilitation in the Treatment of Ischemic Stroke Recovery: A Randomized Controlled Trial

2019 ◽  
Author(s):  
Lixia Li ◽  
Weifeng Zhu ◽  
Guohua Lin ◽  
Chuyun Chen ◽  
Donghui Tang ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Controlled Trial ◽  
Stroke Recovery ◽  
Control Group ◽  
Acupuncture Points ◽  
Modified Ashworth Scale ◽  
Significant Difference ◽  
Scale Scores ◽  
Before And After ◽  
Ashworth Scale

Abstract Background: Acupuncture is a well known treatment for ischemic stroke recovery, but evidence of its effectiveness remains limited. Methods: Rehabilitation training was provided to a control group. In Arm 1 and Arm 2, acupoint schemes were determined by a consensus of acupuncture experts and previous clinical research. Arm 1 was based on an acupoint summary of ancient literature, and Arm 2 was based on the summary of effective acupuncture points from modern RCT literature. Acupuncture was given on the acupoint. After acupuncture, the needles were left in for 30 minutes, and checked every 10 minutes. The three groups received treatment once a day, 5 times a week, for 2 weeks. The primary outcome was the National Institute of Health stroke scale (NIHSS), and the secondary outcomes were the Barthel index (BI) and the modified Ashworth scale (MAS). Outcomes were observed in patients both before and after treatment. Results: A total of 497 patients with ischemic stroke were randomized into either Arm 1 (159 cases), Arm 2 (173 cases) or a control group (165 cases). After 2 weeks of treatment, the NIHSS scores for Arm 1 were lower than those of the control group (P=0.017); the BI scores were higher in Arm 2 than they were in the control group (P=0.020). Additionally, there was no significant difference between Arm 1 and the control group for either the BI scores or the modified Ashworth scale scores (P>0.05). Conclusion: The clinical efficacy of Arm 1 and Arm 2 (acupuncture groups) was superior to that of the control group.

scholarly journals Molecular Genetics of Esophageal Cancer : Indian Perspective

2021 ◽  
Vol 6 (2) ◽  
pp. 155-160
Author(s):  
Suddhasattwa Ray ◽  
Mona Malekzadehmoghani ◽  
Sonia S Ray ◽  
Partha Sen ◽  
Sayan Chakraborty
Keyword(s):  
Promoter Methylation ◽  
Methylation Status ◽  
Cpg Islands ◽  
Stage Iv ◽  
Tissue Samples ◽  
Cpg Dinucleotides ◽  
Specific Pcr ◽  
Significant Difference ◽  
Indian Perspective

Background: RIZ1 is one of the tumor-suppressor genes that is silenced in many human cancers. Change in RIZ1 expression has not been reported in ESCC patients. Therefore, the aim of this study was to investigate the role of RIZ1 in ESCC in the Indian population. Methods: Twelve esophageal squamous-cell carcinoma (ESCC) patients in stage IV and 12 healthy individuals were used in this study. Tissue sampling was taken from individuals and total RNA was isolated and then cDNA was synthesized using PCR. RIZ1 primers were then designed, and RIZ1 expression was quantified by qRT-PCR. Mapping of CpG islands in RIZ1 promoter was performed using bioinformatics tools. The promoter methylation status of this gene was studied using u methylation-specific PCR (MSP). T-student test was used to analyze the data.Results: Decreased RIZ1 expression was observed in ESCC compared with healthy controls. The results showed a relatively higher density of CpG dinucleotides in the RIZ1 promoter. No significant difference in promoter methylation was observed in blood and tissue samples.Conclusion: The study showed a significant down-regulation of RIZ-1 gene in the blood and tissue samples of ESCC patients that did not related to the altered promoter methylation.

scholarly journals Short Term Effect of Virtual Reality on Tear Film Stability and Ocular Discomfort

2020 ◽  
Vol 4 (1) ◽  
pp. 40-46
Author(s):  
Mohd Zulfaezal Che Azemin ◽  
Keyword(s):  
Virtual Reality ◽  
Tear Film ◽  
Film Stability ◽  
The Public ◽  
Non Invasive ◽  
Significant Difference ◽  
Ocular Discomfort ◽  
Tear Film Stability ◽  
Before And After ◽  
Tear Meniscus Height

Virtual reality (VR) has been integrated and used with smartphones as one of the digital entertainments such as in gaming and movie streaming. With emergent of various VR brands in the market, it concerns the public on the possible side effects of VR on the ocular performance specifically on tear film stability and ocular discomfort. The purpose of this study was to compare the change on non-invasive keratograph tear break-up time (NIKBUT), tear meniscus height (TMH) before and after the use of VR for 30 minutes, and to measure the ocular discomfort after the use of VR quantitatively. Thirty-two subjects were recruited in this study and all the subjects were divided randomly into two groups; VR and laptop (used as a control) groups. Each subject needed to watch a movie for 30 minutes using the respective devices. The changes of tear film stability and ocular discomfort before and after the use of the devices were then compared and the results showed that there was no significant difference (p>0.05) after 30 minutes of the use. Comparison of the ocular parameters between the devices on post-watching activities was also not significant (p>0.05). Use of VR did not give any signs of dryness to the eyes and it can be used without any discomfort even after 30 minutes usage of the devices.

Correlation Between Optic Nerve Sheath Diameter and Intracranial Pressure Based on Ultrasonography

2021 ◽  
Vol 11 (1) ◽  
pp. 241-246
Author(s):  
Nifang Pan ◽  
Jie Xie ◽  
Jian He ◽  
Shun Wen ◽  
Chunyang Xu ◽  
...  
Keyword(s):  
Intracranial Pressure ◽  
Time Window ◽  
Optic Nerve Sheath Diameter ◽  
Ultrasound Measurement ◽  
Probe Type ◽  
Monitoring Technology ◽  
Non Invasive ◽  
Minute Period ◽  
Significant Difference ◽  
Before And After

Objective: This paper measures whether there is any difference between the non-invasive monitoring technology that shows the change in ICP value and the current "gold standard" intraventricular probe-type intracranial pressure monitoring technology. Methods: 61 critically ill patients with traumatic brain injury requiring invasive ICP monitoring in the intensive care unit of our hospital from April 2017 to September 2017 were selected. The study subjects were selected in strict accordance with the inclusion and exclusion criteria of experimental design. Each patient recorded the ICP values before and after intravenous drip of 20% mannitol 125 ml, and received ultrasound measurements at bedside by two different physicians before, after, 10 minutes, and 30 minutes after ICP changes. Results: The consistency of the ONSD values measured by different doctors at the same time was the same, and the repeatability of the bedside ultrasound measurement of the ONSD values was better. There was no significant difference between the ONSD value measured immediately after the ICP drop and the ONSD value before the ICP drop. There was a significant difference between the ONSD value measured 10 minutes after the ICP drop and the ONSD value before the ICP drop. However, there was no significant difference in the value of ONSD measured between 10 minutes and 30 minutes after the ICP decreased. Conclusion: ONSD reflects that the lag time window of ICP changes is within 10 minutes, but the measured values of ONSD within the 20-minute period from 10 minutes to 30 minutes after ICP changes can better reflect the current ICP values.

Neopterin Profile to Evaluate the Effectiveness of Treatment in Aggressive Periodontitis

Pteridines ◽  
2003 ◽  
Vol 14 (3) ◽  
pp. 77-81 ◽  
Author(s):  
Aysen Bodur ◽  
Terken Baydar ◽  
Nurdan Ozmeric ◽  
Ayse Basak Engin ◽  
Ahu Uraz ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Periodontal Diseases ◽  
Aggressive Periodontitis ◽  
Periodontal Treatment ◽  
Control Group ◽  
Non Invasive ◽  
Neopterin Concentration ◽  
Significant Difference ◽  
Before And After ◽  
Pre Treatment

Abstract Periodontal disease results from the interaction of the host defence mechanisms with the microbial dental plaque. Analysis of gingival crevicular Huid (GCF) provides a non-invasive means of evaluating the role of the host response in periodontal disease. Based on our previous study, demonstrating increased levels of neopterin in GCF and saliva from patients with aggressive periodontitis (AgP), the aim of this study was to evaluate the effect of periodontal treatment on the levels of neopterin in GCF, saliva and urine of patients with AgP.Pre-treatment values of neopterin in GCF were 4.04 ± 0.86 nmol/ml for the AgP group (n=8) and 2.68 ± 0.90 nmol/ml fyr the control group (n=8; difference not significant). After periodontal treatment, the level of ncoptei in was found 2.38 ± 0.72 nmol/ml in the patient group which did not differ f r om pre-treatment levels. The salivary neopterin concentration was higher in both AgP groups (14.14 ± 2.85 nmol/1 and 8.02 ± 3.12 nmol/1, before and after periodontal treatment) than in controls (2.58 ± 0.3 nmol/1; both p<0.05). No significant difference in salivary neopterin level was observed in patients before and after periodontal treatment.Concentrations of urine neopterin in patients before treatment were 188.5 ± 30.98 μηιοί neopterin/mol creatinine and 168.1 + 20.21 μηιοί in controls (difference not significant). Following periodontal treatment, the urinary neopterin levels (310.1 ± 39.82 μηιοί neopterin/mol creatinine) were higher when compared to the baseline levels and to controls (p<0.05).Our results suggest that neopterin in saliva and GCF might be associated with the periodontal diseases process.

The influence of osteopathic correction on the rate of various rhythms of the body

2019 ◽  
pp. 64-71 ◽  
Author(s):  
A. E. Chernikova ◽  
Yu. P. Potekhina
Keyword(s):  
Heart Rate ◽  
Respiratory Rate ◽  
Age Groups ◽  
Biomechanical Properties ◽  
Dispersion Analysis ◽  
The Body ◽  
Body Tissues ◽  
Age Related ◽  
Significant Difference ◽  
Before And After

Introduction. An osteopathic examination determines the rate, the amplitude and the strength of the main rhythms (cardiac, respiratory and cranial). However, there are relatively few studies in the available literature dedicated to the influence of osteopathic correction (OC) on the characteristics of these rhythms.Goal of research — to study the influence of OC on the rate characteristics of various rhythms of the human body.Materials and methods. 88 adult osteopathic patients aged from 18 to 81 years were examined, among them 30 men and 58 women. All patients received general osteopathic examination. The rate of the cranial rhythm (RCR), respiratory rate (RR) heart rate (HR), the mobility of the nervous processes (MNP) and the connective tissue mobility (CTM) were assessed before and after the OC session.Results. Since age varied greatly in the examined group, a correlation analysis of age-related changes of the assessed rhythms was carried out. Only the CTM correlated with age (r=–0,28; p<0,05) in a statistically significant way. The rank dispersion analysis of Kruskal–Wallis also showed statistically significant difference in this indicator in different age groups (p=0,043). With the increase of years, the CTM decreases gradually. After the OC, the CTM, increased in a statistically significant way (p<0,0001). The RCR varied from 5 to 12 cycles/min in the examined group, which corresponded to the norm. After the OC, the RCR has increased in a statistically significant way (p<0,0001), the MNP has also increased (p<0,0001). The initial heart rate in the subjects varied from 56 to 94 beats/min, and in 15 % it exceeded the norm. After the OC the heart rate corresponded to the norm in all patients. The heart rate and the respiratory rate significantly decreased after the OC (р<0,0001).Conclusion. The described biorhythm changes after the OC session may be indicative of the improvement of the nervous regulation, of the normalization of the autonomic balance, of the improvement of the biomechanical properties of body tissues and of the increase of their mobility. The assessed parameters can be measured quickly without any additional equipment and can be used in order to study the results of the OC.

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