scholarly journals Hybridization of Russian Sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833) and American Paddlefish (Polyodon spathula, Walbaum 1792) and Evaluation of Their Progeny

Genes ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 753 ◽  
Author(s):  
Jenő Káldy ◽  
Attila Mozsár ◽  
Gyöngyvér Fazekas ◽  
Móni Farkas ◽  
Dorottya Lilla Fazekas ◽  
...  
Keyword(s):  
Cell Lineage ◽  
Chromosome Analysis ◽  
Hybrid Origin ◽  
Russian Sturgeon ◽  
Ploidy Levels ◽  
Polyodon Spathula ◽  
Acipenser Gueldenstaedtii ◽  
Calculated Ratio ◽  
Intensive Rearing ◽  
One Year

Two species from the families Acipenseridae and Polyodontidae, Russian sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833; functional tetraploid) and American paddlefish (Polyodon spathula, Walbaum 1792, functional diploid) were hybridized. The hybridization was repeated using eggs from three sturgeon and sperm from four paddlefish individuals. Survival in all hybrid family groups ranged from 62% to 74% 30 days after hatching. This was the first successful hybridization between these two species and between members of the family Acipenseridae and Polyodontidae. Flow cytometry and chromosome analysis revealed two ploidy levels in hybrids. The chromosome numbers of the hybrids ranged between 156–184 and 300–310, in “functional” triploids and “functional” pentaploids, respectively. The hybrid origin and the ploidy levels were also confirmed by microsatellite analyses. In hybrids, the size and the number of dorsal and ventral scutes correlated with the ploidy levels as well as with the calculated ratio of the maternal and paternal chromosome sets. An extra haploid cell lineage was found in three hybrid individuals irrespective of the ploidy level, suggesting polyspermy. Although the growth performance showed high variance in hybrids (mean: 1.2 kg, SD: 0.55), many individuals reached a size of approximately 1 kg by the age of one year under intensive rearing conditions.

scholarly journals Male meiosis and pollen morphology in diploid Indonesian wild bananas and cultivars

The Nucleus ◽  
2021 ◽  
Author(s):  
Fajarudin Ahmad ◽  
Yuyu S. Poerba ◽  
Gert H. J. Kema ◽  
Hans de Jong
Keyword(s):  
Hybrid Sterility ◽  
Pollen Grains ◽  
Chromosome Analysis ◽  
Male Meiosis ◽  
Enzyme Treatment ◽  
Unreduced Gamete ◽  
Ploidy Levels ◽  
Scientific Disciplines ◽  
Sterile Pollen ◽  
Mother Cells

AbstractBreeding of banana is hampered by its genetic complexity, structural chromosome rearrangements and different ploidy levels. Various scientific disciplines, including cytogenetics, linkage mapping, and bioinformatics, are helpful tools in characterising cultivars and wild relatives used in crossing programs. Chromosome analysis still plays a pivotal role in studying hybrid sterility and structural and numerical variants. In this study, we describe the optimisation of the chromosome spreading protocol of pollen mother cells focusing on the effects of standard fixation methods, duration of the pectolytic enzyme treatment and advantages of fluorescence microscopy of DAPI stained cell spreads. We demonstrate the benefits of this protocol on meiotic features of five wild diploid Musa acuminata bananas and a diploid (AA) cultivar banana “Rejang”, with particular attention on pairing configurations and chromosome transmission that may be indicative for translocations and inversions. Pollen slides demonstrate regular-shaped spores except “Rejang”, which shows fertile pollen grains of different size and sterile pollen grains, suggesting partial sterility and unreduced gamete formation that likely resulted from restitutional meiotic divisions.

scholarly journals Substitution of Krill meal for Fish Meal in Feed for Russian Sturgeon, Acipenser gueldenstaedtii

2016 ◽  
Author(s):  
Yang-yang Gong ◽  
Yan-qing Huang ◽  
Lu-jiao Gao ◽  
Jian-xue Lu ◽  
Hong-liang Huang ◽  
...  
Keyword(s):  
Fish Meal ◽  
Russian Sturgeon ◽  
Acipenser Gueldenstaedtii

Functional state of the organism of the hybrid Russian sturgeon × Siberian sturgeon (Acipenser gueldenstaedtii × Acipenser baerii) after winter in cages in Volga Delta

2021 ◽  
pp. 60-69
Author(s):  
Svetlana Sergeevna Astafyeva ◽  
Alexander Robertovich Lozovskiy
Keyword(s):  
Blood Glucose ◽  
Carbohydrate Metabolism ◽  
Functional State ◽  
Total Protein ◽  
Siberian Sturgeon ◽  
Russian Sturgeon ◽  
Acipenser Baerii ◽  
Glucose Content ◽  
Volga Delta ◽  
Acipenser Gueldenstaedtii

Two-year-olds (15 individuals) and five-year-olds (15 individuals) of the Russian sturgeon × Siberian sturgeon (Acipenser gueldenstaedtii × Acipenser baerii) hybrid, kept in net cages in the Volga delta, were studied after wintering in order to establish the functional state of the organism according to the complex of indicators of the erythron system and metabolism substances. The results of the performed study found that the erythron system in five-year-olds is characterized by a higher content of erythrocytes and blood hemoglobin (0.58 ± 0.01×10 12 l –1 and 64.98 ± 1.29 g/l, respectively), compared with two-year-olds (0.49 ± 0.02×10 12 l –1 and 64.98 ± 1.29 g/l, respectively).The serum iron content in both groups was the same. A higher content of total protein and albumin in the blood, as well as a greater activity of ALT were found in five-year-olds, compared with two-year-olds. Thus, the content of total protein and albumin in blood serum in five-year-olds was at the level of 48.23 ± 1.54 and 21.58 ± 2.34 g/l, respectively, and in two-year-olds — 32.42 ± 0.92 and 13,84 ± 0.61 g/l, respectively (p < 0.05). The activity of serum ALT in five-year-olds was 1.07 ± 0.06 μkat/l, and in two-year-olds — 0.85 ± 0.03 μkat/l (p < 0.01). Serum cholesterol in five-year-olds (3.94 ± 0.04 mmol/l) and two-year-olds (4.4 ± 0.08 mmol/l) did not differ significantly (p > 0.05). The content of serum triglycerides in five-year-olds (4.50 ± 0.49 mmol/l) and in two-year-olds (5.36 ± 0.45 mmol/l) did not differ significantly (p > 0.05). The study of carbohydrate metabolism revealed a pronounced variability in the glycemic index in the younger age group (coefficient of variation 44.7 %) with an average blood glucose value of 2.69 ± 0.31 mmol/l. The blood glucose content of five-year-olds was higher than that of two-year-olds — 3.84 ± 0.24 mmol/l (p < 0.05). The established parameters of variation and the age specificity of the indicators of the erythron system, protein, lipid and carbohydrate metabolism can be used to assess the state of physiological adaptation of the hybrid after wintering in cages in the Volga delta.

Genetic analysis of developmental mechanisms in hydra. V. Cell lineage and development of chimera hydra

1978 ◽  
Vol 32 (1) ◽  
pp. 215-232
Author(s):  
T. Sugiyama ◽  
T. Fujisawa
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Interstitial Cell ◽  
Cell Lineage ◽  
Interstitial Cells ◽  
Nerve Cells ◽  
Polyp Size ◽  
Primary Determinant ◽  
One Year ◽  
Almost All

Chimeric hydra were produced by making use of a strain (nf-1) which lacks interstitial cells, nerve cells and nematocytes. This strain arises by spontaneous loss of interstitial cells from its parental strain (sf-1) (Sugiyama & Fujisawa, 1978). Reintroduction of interstitial cells from other strains into nf-1 leads to the creation of chimeric strains that consisted of epithelial cells derived from strain sf-1 and interstitial cells and their derivatives (nerves and nematocytes) from other strains. In chimeras, interstitial or epithelial cells apparently maintain very stable cell lineages; no indication was obtained that suggested interstitial cell differentiation into epithelial cells or dedifferentiation in the opposite direction during the long courses of chimera cultures (up to one year). Developmental characters of chimeras were examined and compared to those of the epithelial cell (sf-1) and the interstitial cell donors. Almost all of the chimera's characters examined (growth rate, budding rate, tentacle numbers, polyp size, regenerative capacity, etc.) closely resembled those of the epithelial cell donor, but not of the interstitial cell donors. This suggests that epithelial cells, rather than interstitial or nerve cells, are the primary determinant of most, if not all, of hydra developmental characters.

Reference Intervals for Blood Analytes of Adult Aquarium‐Housed Russian Sturgeon ( Acipenser gueldenstaedtii )

2020 ◽  
Author(s):  
Stephen E. Cassle ◽  
Roy P.E. Yanong ◽  
Deborah B. Pouder ◽  
Carlos Rodriguez ◽  
Natalie Mylniczenko ◽  
...  
Keyword(s):  
Reference Intervals ◽  
Russian Sturgeon ◽  
Acipenser Gueldenstaedtii ◽  
Blood Analytes
Sign in / Sign up

Export Citation Format

Share Document