scholarly journals Chasing the Apomictic Factors in the Ranunculus auricomus Complex: Exploring Gene Expression Patterns in Microdissected Sexual and Apomictic Ovules

Genes ◽  
2020 ◽  
Vol 11 (7) ◽  
pp. 728 ◽  
Author(s):  
Marco Pellino ◽  
Diego Hojsgaard ◽  
Elvira Hörandl ◽  
Timothy F. Sharbel
Keyword(s):  
Gene Expression ◽  
Asexual Reproduction ◽  
Developmental Stages ◽  
Expression Patterns ◽  
Dominant Factor ◽  
Gene Expression Patterns ◽  
Future Studies ◽  
Dosage Effects ◽  
Differential Gene ◽  
Parent Of Origin

Apomixis, the asexual reproduction via seeds, is associated to polyploidy and hybridization. To identify possible signatures of apomixis, and possible candidate genes underlying the shift from sex to apomixis, microarray-based gene expression patterns of live microdissected ovules at four different developmental stages were compared between apomictic and sexual individuals of the Ranunculus auricomus complex. Following predictions from previous work on mechanisms underlying apomixis penetrance and expressivity in the genus, gene expression patterns were classified into three categories based on their relative expression in apomicts compared to their sexual parental ancestors. We found evidence of misregulation and differential gene expression between apomicts and sexuals, with the highest number of differences detected during meiosis progression and emergence of aposporous initial (AI) cells, a key developmental stage in the ovule of apomicts where a decision between divergent reproductive pathways takes place. While most of the differentially expressed genes (DEGs) could not be annotated, gene expression was classified into transgressive, parent of origin and ploidy effects. Genes related to gametogenesis and meiosis demonstrated patterns reflective of transgressive and genome dosage effects, which support the hypothesis of a dominant factor controlling apomixis in Ranunculus and modulated by secondary modifiers. Three genes with probable functions in sporogenesis and gametogenesis development are identified and characterized for future studies.

scholarly journals Carbohydrate-induced Differential Gene Expression Patterns in the Hyperthermophilic BacteriumThermotoga maritima

2002 ◽  
Vol 278 (9) ◽  
pp. 7540-7552 ◽  
Author(s):  
Swapnil R. Chhabra ◽  
Keith R. Shockley ◽  
Shannon B. Conners ◽  
Kevin L. Scott ◽  
Russell D. Wolfinger ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Differential Gene

Differential gene expression patterns and colocalization of ATP-gated P2X6/P2X4 ion channels during rat small intestine ontogeny

2016 ◽  
Vol 21 (2) ◽  
pp. 81-88 ◽  
Author(s):  
Karla Padilla ◽  
David Gonzalez-Mendoza ◽  
Laura C. Berumen ◽  
Jesica E. Escobar ◽  
Ricardo Miledi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Small Intestine ◽  
Ion Channels ◽  
Differential Gene Expression ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Rat Small Intestine ◽  
Differential Gene

Microarray analysis of gene expression patterns in the leaf during potato tuberization in the potato somatic hybrid Solanum tuberosum and Solanum etuberosum

Genome ◽  
2015 ◽  
Vol 58 (6) ◽  
pp. 305-313 ◽  
Author(s):  
Jagesh Kumar Tiwari ◽  
Sapna Devi ◽  
S. Sundaresha ◽  
Poonam Chandel ◽  
Nilofer Ali ◽  
...  
Keyword(s):  
Gene Expression ◽  
Candidate Genes ◽  
Microarray Analysis ◽  
Somatic Hybrid ◽  
Developmental Stages ◽  
Expression Patterns ◽  
Pcr Analysis ◽  
Gene Expression Patterns ◽  
Potato Tuberization ◽  
Solanum Etuberosum

Genes involved in photoassimilate partitioning and changes in hormonal balance are important for potato tuberization. In the present study, we investigated gene expression patterns in the tuber-bearing potato somatic hybrid (E1-3) and control non-tuberous wild species Solanum etuberosum (Etb) by microarray. Plants were grown under controlled conditions and leaves were collected at eight tuber developmental stages for microarray analysis. A t-test analysis identified a total of 468 genes (94 up-regulated and 374 down-regulated) that were statistically significant (p ≤ 0.05) and differentially expressed in E1-3 and Etb. Gene Ontology (GO) characterization of the 468 genes revealed that 145 were annotated and 323 were of unknown function. Further, these 145 genes were grouped based on GO biological processes followed by molecular function and (or) PGSC description into 15 gene sets, namely (1) transport, (2) metabolic process, (3) biological process, (4) photosynthesis, (5) oxidation-reduction, (6) transcription, (7) translation, (8) binding, (9) protein phosphorylation, (10) protein folding, (11) ubiquitin-dependent protein catabolic process, (12) RNA processing, (13) negative regulation of protein, (14) methylation, and (15) mitosis. RT-PCR analysis of 10 selected highly significant genes (p ≤ 0.01) confirmed the microarray results. Overall, we show that candidate genes induced in leaves of E1-3 were implicated in tuberization processes such as transport, carbohydrate metabolism, phytohormones, and transcription/translation/binding functions. Hence, our results provide an insight into the candidate genes induced in leaf tissues during tuberization in E1-3.

scholarly journals Differential Gene Expression Patterns of EBV Infected EBNA-3A Positive and Negative Human B Lymphocytes

2009 ◽  
Vol 5 (7) ◽  
pp. e1000506 ◽  
Author(s):  
Marie L. Hertle ◽  
Claudia Popp ◽  
Sabine Petermann ◽  
Sabine Maier ◽  
Elisabeth Kremmer ◽  
...  
Keyword(s):  
Gene Expression ◽  
B Lymphocytes ◽  
Differential Gene Expression ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Differential Gene

Photosynthetic Gene Expression in Amaranth, an NAD-ME Type C4 Dicot

1997 ◽  
Vol 24 (4) ◽  
pp. 423 ◽  
Author(s):  
James O. Berry ◽  
Dennis J. McCormac ◽  
John J. Long ◽  
Joseph Boinski ◽  
Amy C. Corey
Keyword(s):  
Gene Expression ◽  
Developmental Stages ◽  
Expression Patterns ◽  
Metabolic Enzyme ◽  
Gene Expression Patterns ◽  
Control Mechanisms ◽  
Multiple Control ◽  
Amaranthus Hypochondriacus ◽  
Specific Accumulation ◽  
Post Transcriptional Regulation

Post-transcriptional regulation determines initial C4 gene expression patterns in developing leaves of Amaranthus hypochondriacus, an NAD-ME type C4 dicot. RuBPCase, PEPCase, and PPdK mRNAs are abundant in meristems and in leaf primordia, but are utilised only during specific developmental stages. While each enzyme shows independent patterns of initial mRNA and polypeptide accumulation, cell-specific localisation of the polypeptides occurs prior to cell-specific localisation of the mRNAs. In mature three-coloured leaves of A. tricolor, loss of photosynthetic activity correlates with reductions in the transcription rates of some plastid-encoded genes, reduction and loss of coordination in the translation of RuBPCase polypeptides, and loss of cell-specific accumulation of RuBPCase mRNAs (but not the polypeptides). The mitochondrial NAD-dependent malic enzyme (NAD-ME) provides an example of a basic metabolic enzyme that has acquired specialised gene expression patterns allowing it to function in the C4 pathway. NAD-ME occurs preferentially in photosynthetic tissues, and is specific to bundle sheath cells throughout development. NAD-ME synthesis is regulated by light and development at transcriptional and post-transcriptional levels. The findings summarised here indicate that C4 genes are independently regulated by multiple control mechanisms in response to developmental, environmental, and metabolic signals.

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