scholarly journals Knockdown of a Novel Gene OsTBP2.2 Increases Sensitivity to Drought Stress in Rice

Genes ◽  
2020 ◽  
Vol 11 (6) ◽  
pp. 629
Author(s):  
Yong Zhang ◽  
Limei Zhao ◽  
Hong Xiao ◽  
Jinkiat Chew ◽  
Jinxia Xiang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Adenosine Diphosphate ◽  
Mitogen Activated Protein Kinase ◽  
Drought Tolerant ◽  
Mitogen Activated Protein ◽  
Phenylpropanoid Biosynthesis ◽  
Peg Treatment ◽  
Use Efficiency ◽  
Protein Kinase Signaling

Drought stress is a major environmental stress, which adversely affects the biological and molecular processes of plants, thereby impairing their growth and development. In the present study, we found that the expression level of OsTBP2.2 which encodes for a nucleus-localized protein member belonging to transcription factor IID (TFIID) family, was significantly induced by polyethylene glycol (PEG) treatment. Therefore, knockdown mutants of OsTBP2.2 gene were generated to investigate the role of OsTBP2.2 in rice response to drought stress. Under the condition of drought stress, the photosynthetic rate, transpiration rate, water use efficiency, and stomatal conductance were significantly reduced in ostbp2.2 lines compared with wild type, Dongjin (WT-DJ). Furthermore, the RNA-seq results showed that several main pathways involved in “MAPK (mitogen-activated protein kinase) signaling pathway”, “phenylpropanoid biosynthesis”, “defense response” and “ADP (adenosine diphosphate) binding” were altered significantly in ostbp2.2. We also found that OsPIP2;6, OsPAO and OsRCCR1 genes were down-regulated in ostbp2.2 compared with WT-DJ, which may be one of the reasons that inhibit photosynthesis. Our findings suggest that OsTBP2.2 may play a key role in rice growth and the regulation of photosynthesis under drought stress and it may possess high potential usefulness in molecular breeding of drought-tolerant rice.

scholarly journals Integrated de novo Analysis of Transcriptional and Metabolic Variations in Salt-Treated Solenostemma argel Desert Plants

2021 ◽  
Vol 12 ◽  
Author(s):  
Hasan Ahmad ◽  
Mohamed Maher ◽  
Eslam M. Abdel-Salam ◽  
Yufei Li ◽  
Chenkun Yang ◽  
...  
Keyword(s):  
Salt Stress ◽  
De Novo ◽  
Mitogen Activated Protein Kinase ◽  
Desert Plant ◽  
Desert Plants ◽  
Mitogen Activated Protein ◽  
Phenylpropanoid Biosynthesis ◽  
Liquid Chromatography Tandem Mass ◽  
Hormonal Signal ◽  
Protein Kinase Signaling

Solenostemma argel (Delile) Hayne is a desert plant that survives harsh environmental conditions with several vital medicinal properties. Salt stress is a major constraint limiting agricultural production around the globe. However, response mechanisms behind the adaptation of S. argel plants to salt stress are still poorly understood. In the current study, we applied an omics approach to explore how this plant adapts to salt stress by integrating transcriptomic and metabolomic changes in the roots and leaves of S. argel plants under salt stress. De novo assembly of transcriptome produced 57,796 unigenes represented by 165,147 transcripts/isoforms. A total of 730 differentially expressed genes (DEGs) were identified in the roots (396 and 334 were up- and down-regulated, respectively). In the leaves, 927 DEGs were identified (601 and 326 were up- and down-regulated, respectively). Gene ontology and Kyoto Encyclopedia of Genes And Genomes pathway enrichment analyses revealed that several defense-related biological processes, such as response to osmotic and oxidative stress, hormonal signal transduction, mitogen-activated protein kinase signaling, and phenylpropanoid biosynthesis pathways are the potential mechanisms involved in the tolerance of S. argel plants to salt stress. Furthermore, liquid chromatography-tandem mass spectrometry was used to detect the metabolic variations of the leaves and roots of S. argel under control and salt stress. 45 and 56 critical metabolites showed changes in their levels in the stressed roots and leaves, respectively; there were 20 metabolites in common between the roots and leaves. Differentially accumulated metabolites included amino acids, polyamines, hydroxycinnamic acids, monolignols, flavonoids, and saccharides that improve antioxidant ability and osmotic adjustment of S. argel plants under salt stress. The results present insights into potential salt response mechanisms in S. argel desert plants and increase the knowledge in order to generate more tolerant crops to salt stress.

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