scholarly journals Comparative Analysis of ANDE 6C Rapid DNA Analysis System and Traditional Methods

Genes ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 582 ◽  
Author(s):  
Michele Ragazzo ◽  
Stefano Melchiorri ◽  
Laura Manzo ◽  
Valeria Errichiello ◽  
Giulio Puleri ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Dna Extraction ◽  
Dna Analysis ◽  
Nuclear Dna ◽  
Forensic Genetics ◽  
Typing Method ◽  
Buccal Swabs ◽  
Pcr Multiplex ◽  
Genetic Profiles ◽  
Rapid Dna Analysis

Rapid DNA analysis is an ultrafast and fully automated DNA-typing system, which can produce interpretable genetic profiles from biological samples within 90 minutes. This “swab in—profile out” method comprises DNA extraction, amplification by PCR multiplex, separation and detection of DNA fragments by capillary electrophoresis. The aim of study was the validation of the Accelerated Nuclear DNA Equipment (ANDE) 6C system as a typing method for reference samples according to the ISO/IEC 17025 standard. Here, we report the evaluation of the validity and reproducibility of results by the comparison of the genetic profiles generated by the ANDE 6C System with those generated by standard technologies. A quantity of 104 buccal swabs were analyzed both through the ANDE 6C technology and the traditional method (DNA extraction and quantification, amplification and separation by capillary electrophoresis). Positive typing was observed in 97% of cases for ANDE 6C technology with only three buccal swabs failing to reveal interpretable signals. Concordance was determined by comparing the allele calls generated by ANDE 6C and conventional technology. Comparison of 2800 genotypes revealed a concordance rate of 99.96%. These results met the ISO/IEC 17025 requirements, enabling us to receive the accreditation for this method. Finally, rapid technology has certainly reached a level of reliability which has made its use in laboratories of forensic genetics a reality.

scholarly journals Genetic DNA Identification from Bone Remains in Kinship Analysis Using Automate Extraction System

2021 ◽  
Author(s):  
Raluca Dumache ◽  
Talida Cut ◽  
Camelia Muresan ◽  
Veronica Ciocan ◽  
Emanuela Stan ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Dna Analysis ◽  
Pcr Amplification ◽  
Human Identification ◽  
Kinship Analysis ◽  
Disaster Victims ◽  
Buccal Swabs ◽  
Bone Powder ◽  
Pcr Products ◽  
Bone Remains

The first ever human identification through DNA analysis was done in the year 1987. Since then, this test has been used, not only in the ruling of civil and juridical cases, but also for human identification of missing persons and mass disaster victims. In this chapter we will present the usefulness of genetic DNA testing of skeletonized remains for human identification, by using automate DNA extraction from three different human bone types: tooth, femur and petrous pyramid. For each case, we obtained saliva samples on buccal swabs from relatives. After the bones were washed and cleaned, Bead Balls Mill Mix 20 (Tehtnica Domel, Slovenia), was used to obtain the bone powder. The DNA extraction from bone samples was performed on the automate Maxwell RSC 48 Instrument (Promega, USA), using the Maxwell FSC DNA IQ Casework Kit (Promega, USA). Power Quant System (Promega, USA) was used for DNA quantification of the samples. The DNA samples were amplified on a Pro Flex PCR System (Thermo Fischer, USA), using the Global Filer PCR Amplification Kit (Applied Biosystems, USA). PCR products were run on a 3500 Genetic Analyzer (Thermo Fischer, USA). Data analysis was performed by Gene Mapper 1.4. Considering that these cases involved DNA extraction from teeth, bones and old human remains, automate system was felt to be the best option to reduce handling errors and increase the possibilities of obtaining good quality DNA.

A rapid salting out method for DNA extraction from buccal swabs

2012 ◽  
Vol 31 (12) ◽  
pp. 1370-1374
Author(s):  
Wei-feng ZHU ◽  
Da-ya LUO ◽  
Shuo TU ◽  
Xia-li ZHANG ◽  
Ke-min JIE ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Salting Out ◽  
Buccal Swabs

scholarly journals Analysis of ancient DNA from a prehistoric Amerindian cemetery

1999 ◽  
Vol 354 (1379) ◽  
pp. 153-159 ◽  
Author(s):  
Anne C. Stone ◽  
Mark Stoneking
Keyword(s):  
Ancient Dna ◽  
Dna Analysis ◽  
Nuclear Dna ◽  
Genetic Research ◽  
Hypervariable Region ◽  
Dna Preservation ◽  
European Contact ◽  
Novel Method ◽  
High Level ◽  
Central Illinois

The Norris Farms No. 36 cemetery in central Illinois has been the subject of considerable archaeological and genetic research. Both mitochondrial DNA (mtDNA) and nuclear DNA have been examined in this 700–year–old population. DNA preservation at the site was good, with about 70% of the samples producing mtDNA results and approximately 15% yielding nuclear DNA data. All four of the major Amerindian mtDNA haplogroups were found, in addition to a fifth haplogroup. Sequences of the first hypervariable region of the mtDNA control region revealed a high level of diversity in the Norris Farms population and confirmed that the fifth haplogroup associates with Mongolian sequences and hence is probably authentic. Other than a possible reduction in the number of rare mtDNA lineages in many populations, it does not appear as if European contact significantly altered patterns of Amerindian mtDNA variation, despite the large decrease in population size that occurred. For nuclear DNA analysis, a novel method for DNA–based sex identification that uses nucleotide differences between the X and Y copies of the amelogenin gene was developed and applied successfully in approximately 20 individuals. Despite the well–known problems of poor DNA preservation and the ever–present possibility of contamination with modern DNA, genetic analysis of the Norris Farms No. 36 population demonstrates that ancient DNA can be a fruitful source of new insights into prehistoric populations.

scholarly journals Maximization of injection volumes for DNA analysis in capillary electrophoresis

2001 ◽  
Vol 22 (20) ◽  
pp. 4328-4332 ◽  
Author(s):  
Chih-Ching Huang ◽  
Ming-Mu Hsieh ◽  
Tai-Chia Chiu ◽  
Yu-Cheng Lin ◽  
Huan-Tsung Chang
Keyword(s):  
Capillary Electrophoresis ◽  
Dna Analysis

scholarly journals Discordance between Mitochondrial and Nuclear DNA Genes Suggests the Possibility of Hybridization of Indian and Southeast Asian Types of Oecophylla smaragdina (Fabricius) (Hymenoptera, Formicidae) in Bangladesh

2020 ◽  
Author(s):  
M. M. Rahman ◽  
S. Hosoishi ◽  
K. Ogata
Keyword(s):  
Dna Analysis ◽  
Nuclear Dna ◽  
Nuclear Gene ◽  
Southeast Asian ◽  
Phylogenetic Study ◽  
Tropical Islands ◽  
Oecophylla Smaragdina ◽  
Mtdna Sequences ◽  
Mitochondrial Dna Analysis ◽  
Shed Light

Background: Oecophylla smaragdina is distributed from India, SE Asia and Australia including many tropical Islands. A recent phylogenetic study based on mitochondrial DNA analysis reveals that Bangladesh is the overlapping zone of both Indian and Southeast Asian type of O. smaragdina. These two different lineages of Indian and SE Asian type have the opportunities of creating the zone of contacts, but no such data was found. In this study, shed light was given to reveal the chance of hybridized colony of O. smaragdina in Bangladesh. Methods: To asses the hybridization scenario, 28 O. smaragdina colony from 27 localities in Bangladesh were analyzed using Longwave length Rhodopsin (LWRh) nuclear gene sequences and was compared with the mtDNA sequences, which was collected from the same localities and deposited into NCBI GenBank. Results: The inconsistency between mitochondrial and nuclear gene types was observed from two colonies of the overlapped zone of contact. These two colonies were identified as SE Asian type by mtDNA analysis however, by nuclear DNA analysis; it was identified as Indian type. These significant discrepancies within the colony suggested the possibility of hybridization of weaver ant in Bangladesh.

scholarly journals Micro-Spectrophotometric Nuclear DNA Analysis of Aspirated Cells from Thyroid Tumor

1981 ◽  
Vol 20 (1) ◽  
pp. 8-14
Author(s):  
Shosaku ABE ◽  
Shoichi INOUE ◽  
Yutaka OHSAKI ◽  
Makoto MURAO ◽  
Mikio ARAKAWA ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Nuclear Dna ◽  
Thyroid Tumor

Optimization of multiplexed PCR on an integrated microfluidic forensic platform for rapid DNA analysis

The Analyst ◽  
2012 ◽  
Vol 137 (23) ◽  
pp. 5510 ◽  
Author(s):  
Matthew D. Estes ◽  
Jianing Yang ◽  
Brett Duane ◽  
Stan Smith ◽  
Carla Brooks ◽  
...  
Keyword(s):  
Dna Analysis ◽  
Multiplexed Pcr ◽  
Rapid Dna Analysis
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