scholarly journals Cytosolic/Plastid Glyceraldehyde-3-Phosphate Dehydrogenase Is a Negative Regulator of Strawberry Fruit Ripening

Genes ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 580 ◽  
Author(s):  
Ya Luo ◽  
Cong Ge ◽  
Min Yang ◽  
Yu Long ◽  
Mengyao Li ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Reduced Glutathione ◽  
Expression Patterns ◽  
Plant Stress ◽  
Negative Regulator ◽  
Strawberry Fruit ◽  
Catalytic Function ◽  
Strawberry Fruit Ripening ◽  
Transient Overexpression ◽  
H2o2 Level

Cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPC) and plastid glyceraldehyde-3-phosphate dehydrogenase (GAPCp) are key enzymes in glycolysis. Besides their catalytic function, GAPC/GAPCp participates in the regulation of plant stress response and growth and development. However, the involvement of GAPC/GAPCp in the regulation of fruit ripening is unclear. In this study, FaGAPC2 and FaGAPCp1 in strawberries were isolated and analyzed. FaGAPC2 and FaGAPCp1 transcripts showed high transcript levels in the fruit. Transient overexpression of FaGAPC2 and FaGAPCp1 delayed fruit ripening, whereas RNA interference promoted fruit ripening and affected fruit anthocyanins and sucrose levels. Change in the expression patterns of FaGAPC2 and FaGAPCp1 also influenced the expression of several glycolysis-related and ripening-related genes such as CEL1, CEL2, SS, ANS, MYB5, NCED1, ABI1, ALDO, PK, and G6PDH, and H2O2 level and reduced glutathione (GSH)/glutathione disulfide (GSSG) redox potential. Meanwhile, metabolomics experiments showed that transient overexpression of FaGAPCp1 resulted in a decrease in anthocyanins, flavonoids, organic acid, amino acids, and their derivatives. In addition, abscisic acid (ABA) and sucrose treatment induced the production of large amounts of H2O2 and inhibited the expression of FaGAPC2/FaGAPCp1 in strawberry fruit. These results revealed that FaGAPC2/FaGAPCp1 is a negative regulator of ABA and sucrose mediated fruit ripening which can be regulated by oxidative stress.

scholarly journals The long noncoding RNA FRILAIR regulates strawberry fruit ripening by functioning as a noncanonical target mimic

PLoS Genetics ◽  
2021 ◽  
Vol 17 (3) ◽  
pp. e1009461
Author(s):  
Yajun Tang ◽  
Zhipeng Qu ◽  
Jiajun Lei ◽  
Reqing He ◽  
David L. Adelson ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Noncoding Rna ◽  
Expression Patterns ◽  
Noncoding Rnas ◽  
Mrna Levels ◽  
Strawberry Fruit ◽  
Rna Seq ◽  
Fruit Maturation ◽  
Temporal Expression ◽  
Strawberry Fruit Ripening

Long noncoding RNAs (lncRNAs) are emerging as important regulators in plant development, but few of them have been functionally characterized in fruit ripening. Here, we have identified 25,613 lncRNAs from strawberry ripening fruits based on RNA-seq data from poly(A)-depleted libraries and rRNA-depleted libraries, most of which exhibited distinct temporal expression patterns. A novel lncRNA, FRILAIR harbours the miR397 binding site that is highly conserved in diverse strawberry species. FRILAIR overexpression promoted fruit maturation in the Falandi strawberry, which was consistent with the finding from knocking down miR397, which can guide the mRNA cleavage of both FRILAIR and LAC11a (encoding a putative laccase-11-like protein). Moreover, LAC11a mRNA levels were increased in both FRILAIR overexpressing and miR397 knockdown fruits, and accelerated fruit maturation was also found in LAC11a overexpressing fruits. Overall, our study demonstrates that FRILAIR can act as a noncanonical target mimic of miR397 to modulate the expression of LAC11a in the strawberry fruit ripening process.

scholarly journals Type 2C protein phosphatase ABI1 is a negative regulator of strawberry fruit ripening

2013 ◽  
Vol 64 (6) ◽  
pp. 1677-1687 ◽  
Author(s):  
Hai-feng Jia ◽  
Dong Lu ◽  
Jing-hua Sun ◽  
Chun-li Li ◽  
Yu Xing ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Protein Phosphatase ◽  
Negative Regulator ◽  
Strawberry Fruit ◽  
Strawberry Fruit Ripening

scholarly journals A Method for Assaying of Protein Kinase Activity In Vivo and Its Use in Studies of Signal Transduction in Strawberry Fruit Ripening

2021 ◽  
Vol 22 (19) ◽  
pp. 10495
Author(s):  
Wei Wang ◽  
Zhengrong Dai ◽  
Jie Li ◽  
Jinyao Ouyang ◽  
Tianyu Li ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Protein Kinase ◽  
Fruit Ripening ◽  
Kinase Activity ◽  
Expression Patterns ◽  
Protein Kinase Activity ◽  
Strawberry Fruit ◽  
Simple Method ◽  
Strawberry Fruit Ripening

Strawberry (Fragaria × ananassa) fruit ripening is regulated by a complex of cellular signal transduction networks, in which protein kinases are key components. Here, we report a relatively simple method for assaying protein kinase activity in vivo and specifically its application to study the kinase, FaMPK6, signaling in strawberry fruit. Green fluorescent protein (GFP)-tagged FaMPK6 was transiently expressed in strawberry fruit and after stimuli were applied to the fruit it was precipitated using an anti-GFP antibody. The precipitated kinase activity was measured in vitro using 32P-ATP and myelin basic protein (MBP) as substrates. We also report that FaMPK6 is not involved in the abscisic acid (ABA) signaling cascade, which is closely associated with FaMPK6 signaling in other plant species. However, methyl jasmonate (MeJA), low temperature, and high salt treatments were all found to activate FaMPK6. Transient manipulation of FaMPK6 expression was observed to cause significant changes in the expression patterns of 2749 genes, of which 264 were associated with MeJA signaling. The data also suggest a role for FaMPK6 in modulating cell wall metabolism during fruit ripening. Taken together, the presented method is powerful and its use will contribute to a profound exploration to the signaling mechanism of strawberry fruit ripening.

scholarly journals Downregulation of RdDM during strawberry fruit ripening

2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Jingfei Cheng ◽  
Qingfeng Niu ◽  
Bo Zhang ◽  
Kunsong Chen ◽  
Ruihua Yang ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Strawberry Fruit ◽  
Strawberry Fruit Ripening

Melatonin accelerates strawberry fruit ripening by triggering GAMYB gene expression and promoting ABA accumulation

2021 ◽  
Vol 281 ◽  
pp. 109919
Author(s):  
Sirvan Mansouri ◽  
Hassan Sarikhani ◽  
Mohammad Sayyari ◽  
Morteza Soleimani Aghdam
Keyword(s):  
Gene Expression ◽  
Fruit Ripening ◽  
Strawberry Fruit ◽  
Strawberry Fruit Ripening

MAPK5 and MAPK10 overexpression influences strawberry fruit ripening, antioxidant capacity and resistance to Botrytis cinerea

Planta ◽  
2021 ◽  
Vol 255 (1) ◽  
Author(s):  
Yunting Zhang ◽  
Yu Long ◽  
Yiting Liu ◽  
Min Yang ◽  
Liangxin Wang ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Botrytis Cinerea ◽  
Fruit Ripening ◽  
Strawberry Fruit ◽  
Strawberry Fruit Ripening

scholarly journals Ethylene is involved in strawberry fruit ripening in an organ-specific manner

2013 ◽  
Vol 64 (14) ◽  
pp. 4421-4439 ◽  
Author(s):  
Catharina Merchante ◽  
José G. Vallarino ◽  
Sonia Osorio ◽  
Irene Aragüez ◽  
Natalia Villarreal ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Strawberry Fruit ◽  
Specific Manner ◽  
Organ Specific ◽  
Strawberry Fruit Ripening

scholarly journals Brassinosteroid is involved in strawberry fruit ripening

2012 ◽  
Vol 69 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Ye-mao Chai ◽  
Qing Zhang ◽  
Lin Tian ◽  
Chun-Li Li ◽  
Yu Xing ◽  
...  
Keyword(s):  
Fruit Ripening ◽  
Strawberry Fruit ◽  
Strawberry Fruit Ripening
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