scholarly journals Ecological Processes Affecting Long-Term Eukaryote and Prokaryote Biofilm Persistence in Nitrogen Removal from Sewage

Genes ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 449 ◽  
Author(s):  
Inga Leena Angell ◽  
Linda Bergaust ◽  
Jon Fredrik Hanssen ◽  
Else Marie Aasen ◽  
Knut Rudi

The factors affecting long-term biofilm stability in sewage treatment remain largely unexplored. We therefore analyzed moving bed bioreactors (MBBRs) biofilm composition and function two years apart from four reactors in a nitrogen-removal sewage treatment plant. Multivariate ANOVA revealed a similar prokaryote microbiota composition on biofilm carriers from the same reactors, where reactor explained 84.6% of the variance, and year only explained 1.5%. Eukaryotes showed a less similar composition with reactor explaining 56.8% of the variance and year 9.4%. Downstream effects were also more pronounced for eukaryotes than prokaryotes. For prokaryotes, carbon source emerged as a potential factor for deterministic assembly. In the two reactors with methanol as a carbon source, the bacterial genus Methylotenera dominated, with M. versatilis as the most abundant species. M. versatilis showed large lineage diversity. The lineages mainly differed with respect to potential terminal electron acceptor usage (nitrogen oxides and oxygen). Searches in the Sequence Read Archive (SRA) database indicate a global distribution of the M. versatilis strains, with methane-containing sediments as the main habitat. Taken together, our results support long-term prokaryote biofilm persistence, while eukaryotes were less persistent.

1999 ◽  
Vol 50 (7) ◽  
pp. 689 ◽  
Author(s):  
T. H. Donnelly ◽  
P. W. Ford ◽  
D. McGregor ◽  
D. Allen

Anthropogenic changes detected in the sediment of a shallow (~1.6 m) billabong (Horseshoe Lagoon) are the result of a Sewage Treatment Plant (STP) connection in 1958 and catchment urbanization around 1974. Nutrient-rich water caused the collapse of macrophyte populations, algal blooms became common, and urbanization increased sediment deposition (~1 cm year −1 ). Changes in P retention were investigated by using dated cores, pore-water element profiles, and water quality records. The lagoon is the last of three STP holding ponds. Ponds 1 and 2 were sand-mining pits; they receive no sediment input and have negligible P adsorption. The Fe:P ratios in sediments from three Australian rivers have a common slope and this relationship was used to examine Fe:P ratios in the anoxic bottom sediments of the lagoon. A potential for effluent P adsorption developed in the lagoon through Fe mobilization and enrichment in the upper sediments and a strong clay–Fe–P association. The mean long- term P adsorption in the lagoon (35±18%) was the result of maintaining clay input to an oxic waterbody. Bacterial sulfate reduction is the main process decreasing available Fe for effluent P adsorption. Appropriately designed systems could expect to maintain effluent P adsorption efficiencies of around 70%.


2013 ◽  
Vol 67 (10) ◽  
pp. 2273-2280 ◽  
Author(s):  
Y. Dai ◽  
A. Constantinou ◽  
P. Griffiths

The Beaudesert Sewage Treatment Plant (STP), originally built in 1966 and augmented in 1977, is a typical biological trickling filter (TF) STP comprising primary sedimentation tanks (PSTs), TFs and humus tanks. The plant, despite not originally being designed for nitrogen removal, has been consistently achieving over 60% total nitrogen reduction and low effluent ammonium concentration of less than 5 mg NH3-N/L. Through the return of a NO3−-rich stream from the humus tanks to the PSTs and maintaining an adequate sludge age within the PSTs, the current plant is achieving a substantial degree of denitrification. Further enhanced denitrification has been achieved by raising the recycle flows and maintaining an adequate solids retention time (SRT) within the PSTs. This paper describes the approach to operating a TF plant to achieve a high degree of nitrification and denitrification. The effectiveness of this approach is demonstrated through the pilot plant trial. The results from the pilot trial demonstrate a significant improvement in nitrogen removal performance whilst maximising the asset life of the existing infrastructure. This shows great potential as a retrofit option for small and rural communities with pre-existing TFs that require improvements in terms of nitrogen removal.


Energies ◽  
2019 ◽  
Vol 12 (12) ◽  
pp. 2424 ◽  
Author(s):  
Chia-Chi Chang ◽  
Manh Van Do ◽  
Wei-Li Hsu ◽  
Bo-Liang Liu ◽  
Ching-Yuan Chang ◽  
...  

Combined heat and power production from biogas is now playing an important role in energy and resource utilization as well as pollution control in waste water treatment. This research used biogas from the Bali Sewage Treatment Plant in New Taipei City, Taiwan, as a major source of fuel for the electricity generation. A micro gas turbine electricity generator, Capstone CR-30, which possesses a maximum rated power load (PWL) of 30 kW, was equipped to convert biogas into electricity. The biogas is mainly composed of CH4 (56.1 ± 8.0 vol.%), CO2 (25.5 ± 9.8 vol.%), H2 (0.5 vol.%), and H2S (0.99 ± 0.07 ppmv). During the test operation period of the generator, it was found that the thermal efficiency increases from 19.8% to 23.4% kWhe/kWhth, while the electricity generation efficiency (ηEB) also rises from 0.93 to 1.09 kWhe/m3 biogas as the PWL increases from 10 kW to 30 kW. The results indicated that the generator has a better performance with higher PWL. At PWL = 30 kW, the average adjusted concentrations of CO and NOx (adjusted to 15 vol.% O2) emitted from the generator are 86 ppmv and 17 ppmv, respectively. Both are much lower than the emission standards of stationary sources in Taiwan of 2000 ppmv and 150 ppmv, respectively. Thus, PWL of 30 kW was selected in cooperation with biogas inflow = 0.412 m3/min and air/fuel ratio (i.e., air/biogas ratio) = 76.0 vol./vol. for the long-term regular operation. At the above setting conditions for long-term operation, the generator continuously consumed the biogas and provided stable electricity generation at a rate of 19.64 kWhe/h for a 2-year running period. Moreover, the greenhouse gas can be cut off with a rate of 10.78 kg CO2e/h when using biogas as fuel for electricity generation. Overall, this research proves that the application of a micro gas turbine electricity generator not only has promising performance for using biogas but also gives a significant reduction of greenhouse gas emission, which fits the concepts of the circular economy and environmental protection.


1992 ◽  
Vol 26 (5-6) ◽  
pp. 1315-1323 ◽  
Author(s):  
H. Aspegren ◽  
B. Andersson ◽  
U. Nyberg ◽  
J. la C. Jansen

Optimization of wastewater treatment plants with extensive phosphorus and nitrogen removal is complicated. The Klagshamn wastewater treatment plant in Sweden is operated with pre-precipitation of phosphorus with ferric chloride and denitrification with methanol as carbon source. An activated sludge process, operated with pre-precipitation and denitrification with external carbon source in a compartmentalized plant, requires only small tank volumes but increases the need for proper operation and optimization. On-line nitrogen, ammonia, and TOC sensors are used for a day-to-day control and optimization while mathematical modelling is used for long term strategic planning. The on-line measurements are further used as the basis for the modelling. TOC and ammonia sensors at the influent clearly identify typical and extreme loading variations and nitrate measurements in the activated sludge tanks and the effluent shows the dynamics of the processes. These measurements provide a basis for model calibration. In combination low residuals of nitrogen, phosphorus and organic matter can be achieved.


Author(s):  
Kyunghoi Kim ◽  
Kyunghoi Kim ◽  
Oh Seok Jin ◽  
Oh Seok Jin ◽  
In-Cheol Lee ◽  
...  

For the better understanding of long-term variations of water quality in Jinhae Bay after establishment of special law, we analyzed the archive data monitored in Jinhae Bay during the last 17 years (1997-2013). And change on fish catch due to the variations of water quality was investigated. A marked decrease in the number of red tide occurrence is due to the effectiveness of the law and sewage treatment plant that has targeted the reduction of COD in the effluent water since early 2000. Although the improvement of water quality, increase in fishery production was not observed in Jinhae Bay. For the recovery of fishery production, processes for restoration of entire ecosystem such as restoration of artificial intertidal flat and seaweed bed and remediation of organic-rich sea bed should be accompanied with improvement of water quality.


1990 ◽  
Vol 22 (7-8) ◽  
pp. 77-84
Author(s):  
J. Hultgren ◽  
L.-G. Reinius ◽  
M. Tendaj

The purification requirements for the Stockholm sewage treatment plants will become more stringent in the future. The expected limit values for the effluent, expressed as annual mean values, are for BOD7, Tot-P, and Tot-N, 10, 0.3 and 15 mg/l respectively. If these contents are multiplied by the design flow values for the three plants, we obtain the maximum quantities which may be released. If the relevant authorities permit the municipality to distribute these total quantities as desired between the three plants, future necessary extensions can be optimized. The following main principles apply to an extension of the three plants: Loudden sewage treatment plant: This comparatively small treatment plant could, if the requirements are lower than in the other two plants, continue in operation with no other extensions than the inclusion of anoxic zones. It would, however, be necessary to refurbish the plant after a number of years of neglected maintenance. Bromma sewage treatment plant: The biological stage was extended during the 1982-84 period. For this reason, the municipality suggests that no further extensions of the aeration tanks be required, before 1995 at the earliest. A nitrogen removal with outgoing contents of Tot-N of 15-17 mg/l is expected to be achieved by measures taken to reduce the load on the biological stage instead. These measures consist of centrifuging the excess sludge and pumping it directly to the digesters instead of returning it to the inlet. Furthermore, separate treatment of the reject water from the sludge centrifuges is planned. A third measure could be changing over to a more efficient precipitation chemical to permit a further reduction of the load on the biological stage with regard to, inter alia, BOD7, Tot-N etc. To meet the requirements for phosphorus removal (0.3 mg/l), the plant will be extended with a filter stage after the existing biological stage. Henriksdal sewage treatment plant: At this plant, which is the largest of the three, the largest extensions are planned. To meet the requirements for nitrogen removal, the present volumes in the aeration tanks will be tripled and will be utilized as anoxic and aerated zones as required. Three new lines with aeration tanks and secondary sedimentation tanks will be constructed. The existing aeration tanks will also be deepened from 5 to 12 m. The requirements for low phosphorus contents in the effluent will be met by installing a filter stage, as in the Bromma plant.


1990 ◽  
Vol 22 (1-2) ◽  
pp. 239-250 ◽  
Author(s):  
B. Andersson

A test program for the use of fixed bed processes in systems for nitrogen removal at an advanced sewage treatment plant is described. Results from studies on nitrification in a full scale trickling filter plant with different filter depths and at different wastewater temperatures are presented. Results from full scale experiments with denitrification/nitrification in a retrofitted activated sludge plant are also presented. The effect of an aerated submerged fixed bed in the aeration basin on nitrification was investigated. Observations of the biofilm formed on the fixed bed were made in microscope.


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