scholarly journals Genome-Wide Identification of the VQ Protein Gene Family of Tobacco (Nicotiana tabacum L.) and Analysis of Its Expression in Response to Phytohormones and Abiotic and Biotic Stresses

Genes ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 284
Author(s):  
Cuihua Liu ◽  
Hai Liu ◽  
Changyong Zhou ◽  
Michael P. Timko
Keyword(s):  
Amino Acid ◽  
Nicotiana Tabacum ◽  
Plant Growth ◽  
Gene Family ◽  
Control Plant ◽  
In Silico Analysis ◽  
Differentially Expressed ◽  
Main Element ◽  
Mechanical Wounding ◽  
Nicotiana Tabacum L

VQ motif-containing proteins (VQ proteins) are transcriptional regulators that work independently or in combination with other transcription factors (TFs) to control plant growth and development and responses to biotic and abiotic stresses. VQ proteins contain a conserved FxxhVQxhTG amino acid motif that is the main element of its interaction with WRKY TFs. We identified 59 members of the tobacco (Nicotiana tabacum L.) NtVQ gene family by in silico analysis and examined their differential expression in response to phytohormonal treatments and following exposure to biotic and abiotic stressors. NtVQ proteins clustered into eight groups based upon their amino acid sequence and presence of various conserved domains. Groups II, IV, V, VI, and VIII contained the largest proportion of NtVQ gene family members differentially expressed in response to one or more phytohormone, and NtVQ proteins with similar domain structures had similar patterns of response to different phytohormones. NtVQ genes differentially expressed in response to temperature alterations and mechanical wounding were also identified. Over half of the NtVQ genes were significantly induced in response to Ralstonia solanacearum infection. This first comprehensive characterization of the NtVQ genes in tobacco lays the foundation for further studies of the NtVQ-mediated regulatory network in plant growth, developmental, and stress-related processes.

Amino-acid-transport mutant of Nicotiana tabacum L.

Planta ◽  
1985 ◽  
Vol 166 (1) ◽  
pp. 141-144 ◽  
Author(s):  
A. C. Borstlap ◽  
J. Schuurmans ◽  
J.-P. Bourgin
Keyword(s):  
Amino Acid ◽  
Nicotiana Tabacum ◽  
Amino Acid Transport ◽  
Acid Transport ◽  
Nicotiana Tabacum L ◽  
Transport Mutant

CHANGES IN LEAF PIGMENTS DURING SENESCENCE AND CURING OF FLUE-CURED TOBACCO

1984 ◽  
Vol 64 (1) ◽  
pp. 229-232 ◽  
Author(s):  
WILLIAM A. COURT ◽  
JOHN G. HENDEL
Keyword(s):  
Nicotiana Tabacum ◽  
Plant Growth ◽  
Chlorophyll A ◽  
Chlorophyll B ◽  
Nicotiana Tabacum L ◽  
Pigment Concentrations ◽  
Oxygen Substitution ◽  
Β Carotene ◽  
Carotenoid Degradation

Neoxanthin, violaxanthin, lutein, β-carotene, chlorophyll a and chlorophyll b in the leaves of flue-cured tobacco (Nicotiana tabacum L.) were determined in samples collected at intervals from the middle of July through harvest. Harvested leaves were also sampled at intervals during flue curing for pigment determinations. Except where interrupted by rainfall or irrigation, pigment concentrations progressively declined during plant growth; this degradation was accelerated during flue curing. Carotenoid degradation during flue curing was proportional to the degree of oxygen substitution of the carotenoid. Chlorophyll a and chlorophyll b in cured tissue were typically less than 1% of the amounts present at harvest.Key words: Carotenoids, chlorophyll, tobacco (flue-cured), flue curing, senescence

scholarly journals Overexpression of chitinase gene with a GC-rich synthetic enhancer in tobacco plant (Nicotiana tabacum L.) Overekspresi gen kitinase dengan enhancer sintetis kaya GC pada tanaman tembakau (Nicotiana tabacum L.)

2016 ◽  
Vol 68 (2) ◽  
Author(s):  
D SANTOSO ◽  
T CHAIDAMSARI ◽  
A BUDIANI ◽  
H MINARSIH ◽  
S DWI UTOMO ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Nicotiana Tabacum ◽  
Transgenic Tobacco ◽  
Recombinant Dna ◽  
Tobacco Plant ◽  
Control Plant ◽  
Chitinase Activity ◽  
Dot Blot ◽  
Nicotiana Tabacum L ◽  
Hindiii Restriction Site

Ringkasan Perakitan tanaman perkebunan toleran terhadap serangan cendawan patogenik  dilakukan dengan mengoverekspresikan gen penyandi kitinase. Untuk itu elemen DNA peningkat ekspresi (enhancer E52) yang berupa oligonukleotida 52 pb dan kaya kandungan basa purin (GC) disisipkan di ujung 5’ konstruk 35S-chi. Penyisipan E52 tersebut dilakukan secara lebih terarah pada situs ganda HindIII-SalI dari MCS pCAMBIA2301. Melalui situs HindIII yang terletak tepat di ujung 3’ E52, konstruk 35S-chi kemudian disambungkan dengan E52 pada pCAMBIA tersebut. Transformasi DNA rekombinan ke dalam sel tembakau dikerjakan melalui perantaraan Agrobacterium tumefaciens LBA4404. Sel tanaman transgenik     diseleksi    dan  diregenrasi dalam media  yang    mengandung  3%  sukrosa, 0,5 mg/L diregenerasikan pada media   MS   padat  benzilaminopurin   (BAP)  dan  50 mg/L kanamisin. Pada media ini tunas transgenik    tembakau   mulai   terbentuk    setelah 5 minggu penanaman. Analisis  tingkat aktivitas enzimatis menunjukkan bahwa aktivitas kitinase pada tembakau transgenik 40 hingga 80 kali lebih tinggi daripada non-transgenik. Pengujian hibridisasi protein menggunakan antibodi anti-kitinase, dot blot dan western blot, membuktikan bahwa enhancer tersebut dapat meningkatkan ekspresi transgen chi pada tanaman tembakau.Summary Development of estate crops tolerant to pathogenic fungi is conducted by overexpressing chi gene. For this purpose, a synthetic enhancer consisting of 52 base pairs and GC-rich was inserted at immediate 5’ end of a 35S-chi cassette. Insertion of the E52 was directed at HindIII-SalI restriction sites of  the pCAMBIA2301 MCS.  With HindIII restriction site located just after the 3’ end of the E52 sequence, the 35S-chi construct was then ligated with the E52 of the pCAMBIA. Transformation of the resulting recombinant DNA into tobacco cells was mediated by Agrobacterium tumefaciens LBA4404. The transgenic cells were selected and regenerated on a solid MS medium supplemented with 3% sucrose, 0.5 mg/L benzylamino purine (BAP) and 50 mg/L kanamycin. Tobacco   shoots   were   initiated  after 5 weeks inoculation on the selection media. Enzymatic analysis demonstrated that chitinase activity of transgenic tobacco was 40 to 80 folds higher than that of the control plant. Analysis of  enzymatic activity using hybridization with anti-chitinase antibody indicated that the level of chitinase activity in the transgenic tobacco carrying the enhancer is higher than that  without enhancer. These data suggest that the enhancer improved the expression of chi transgene in tobacco. 

scholarly journals Overexpression of chitinase gene with a GC-rich synthetic enhancer in tobacco plant (Nicotiana tabacum L.) Overekspresi gen kitinase dengan enhancer sintetis kaya GC pada tanaman tembakau (Nicotiana tabacum L.)

2016 ◽  
Vol 68 (2) ◽  
Author(s):  
D SANTOSO ◽  
T CHAIDAMSARI ◽  
A BUDIANI ◽  
H MINARSIH ◽  
S DWI UTOMO ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Nicotiana Tabacum ◽  
Transgenic Tobacco ◽  
Recombinant Dna ◽  
Tobacco Plant ◽  
Control Plant ◽  
Chitinase Activity ◽  
Dot Blot ◽  
Nicotiana Tabacum L ◽  
Hindiii Restriction Site

Ringkasan Perakitan tanaman perkebunan toleran terhadap serangan cendawan patogenik  dilakukan dengan mengoverekspresikan gen penyandi kitinase. Untuk itu elemen DNA peningkat ekspresi (enhancer E52) yang berupa oligonukleotida 52 pb dan kaya kandungan basa purin (GC) disisipkan di ujung 5’ konstruk 35S-chi. Penyisipan E52 tersebut dilakukan secara lebih terarah pada situs ganda HindIII-SalI dari MCS pCAMBIA2301. Melalui situs HindIII yang terletak tepat di ujung 3’ E52, konstruk 35S-chi kemudian disambungkan dengan E52 pada pCAMBIA tersebut. Transformasi DNA rekombinan ke dalam sel tembakau dikerjakan melalui perantaraan Agrobacterium tumefaciens LBA4404. Sel tanaman transgenik     diseleksi    dan  diregenrasi dalam media  yang    mengandung  3%  sukrosa, 0,5 mg/L diregenerasikan pada media   MS   padat  benzilaminopurin   (BAP)  dan  50 mg/L kanamisin. Pada media ini tunas transgenik    tembakau   mulai   terbentuk    setelah 5 minggu penanaman. Analisis  tingkat aktivitas enzimatis menunjukkan bahwa aktivitas kitinase pada tembakau transgenik 40 hingga 80 kali lebih tinggi daripada non-transgenik. Pengujian hibridisasi protein menggunakan antibodi anti-kitinase, dot blot dan western blot, membuktikan bahwa enhancer tersebut dapat meningkatkan ekspresi transgen chi pada tanaman tembakau.Summary Development of estate crops tolerant to pathogenic fungi is conducted by overexpressing chi gene. For this purpose, a synthetic enhancer consisting of 52 base pairs and GC-rich was inserted at immediate 5’ end of a 35S-chi cassette. Insertion of the E52 was directed at HindIII-SalI restriction sites of  the pCAMBIA2301 MCS.  With HindIII restriction site located just after the 3’ end of the E52 sequence, the 35S-chi construct was then ligated with the E52 of the pCAMBIA. Transformation of the resulting recombinant DNA into tobacco cells was mediated by Agrobacterium tumefaciens LBA4404. The transgenic cells were selected and regenerated on a solid MS medium supplemented with 3% sucrose, 0.5 mg/L benzylamino purine (BAP) and 50 mg/L kanamycin. Tobacco   shoots   were   initiated  after 5 weeks inoculation on the selection media. Enzymatic analysis demonstrated that chitinase activity of transgenic tobacco was 40 to 80 folds higher than that of the control plant. Analysis of  enzymatic activity using hybridization with anti-chitinase antibody indicated that the level of chitinase activity in the transgenic tobacco carrying the enhancer is higher than that  without enhancer. These data suggest that the enhancer improved the expression of chi transgene in tobacco. 

scholarly journals Profiling of MicroRNAs Involved in Mepiquat Chloride-Mediated Inhibition of Internode Elongation in Cotton (Gossypium hirsutum L.) Seedlings

2021 ◽  
Vol 12 ◽  
Author(s):  
Li Wang ◽  
Ying Yin ◽  
Xiuxiu Jing ◽  
Menglei Wang ◽  
Miao Zhao ◽  
...  
Keyword(s):  
Plant Growth ◽  
Gossypium Hirsutum ◽  
Growth Inhibition ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Control Plant ◽  
Differentially Expressed ◽  
Internode Elongation ◽  
Mepiquat Chloride ◽  
Differentially Expressed Mirnas

Mepiquat chloride (MC) is the most important plant growth retardant that is widely used in cotton (Gossypium hirsutum L.) production to suppress excessive vegetative growth and improve plant architecture. MicroRNAs (miRNAs) are important gene expression regulators that control plant growth and development. However, miRNA-mediated post-transcriptional regulation in MC-induced growth inhibition remains unclear. In this study, the dynamic expression profiles of miRNAs responsive to MC in cotton internodes were investigated. A total of 508 known miRNAs belonging to 197 families and five novel miRNAs were identified. Among them, 104 miRNAs were differentially expressed at 48, 72, or 96 h post MC treatment compared with the control (0 h); majority of them were highly conserved miRNAs. The number of differentially expressed miRNAs increased with time after treatment. The expression of 14 known miRNAs was continuously suppressed, whereas 12 known miRNAs and one novel miRNA were continuously induced by MC. The expression patterns of the nine differentially expressed miRNAs were verified using qRT-PCR. The targets of the known and novel miRNAs were predicted. Four conserved and six novel targets were validated using the RLM-5′ RACE assay. This study revealed that miRNAs play crucial regulatory roles in the MC-induced inhibition of internode elongation. It can improve our understanding of post-transcriptional gene regulation in MC-mediated growth inhibition and could potentially facilitate the breeding of dwarf cotton.

scholarly journals Differential Induction of a Peroxidase Gene Family During Infection of Rice by Xanthomonas oryzae pv. oryzae

1997 ◽  
Vol 10 (7) ◽  
pp. 861-871 ◽  
Author(s):  
Jaishree M. Chittoor ◽  
Jan E. Leach ◽  
Frank F. White
Keyword(s):  
Amino Acid ◽  
Gene Family ◽  
Genomic Library ◽  
Rice Genome ◽  
Bacterial Pathogen ◽  
Amino Acid Sequences ◽  
Xanthomonas Oryzae ◽  
Mechanical Wounding ◽  
Peroxidase Gene

Induction of peroxidase has been correlated with resistant interactions between rice and Xanthomonas oryzae pv. oryzae. To assist in analysis of the role of rice peroxidases in plant defense against the bacterial pathogen, three peroxidase genes, POX22.3, POX8.1, and POX5.1, were identified from a rice cDNA library that was constructed from leaves of plants undergoing a resistant reaction. These genes were highly similar in nucleic acid and amino acid sequences and belonged to a gene family. The three genes showed differential expression in infiltrated rice leaves during pathogen interactions and mechanical stress. Only two peroxidase genes, POX8.1 and POX22.3, were predominantly expressed during resistant interactions. These two genes also were expressed during susceptible interactions, but induction was delayed compared with resistant interactions. POXgX9, a fourth peroxidase gene that was isolated from a genomic library, is adjacent to POX22.3 in the rice genome and has greater than 90% similarity in nucleotide and amino acid sequence identity to POX22.3. Interestingly, POXgX9 was expressed only in the roots of rice plants. While POX22.3 was expressed in both leaves and roots, POX8.1 and POX5.1 were not detected in roots but were induced in leaves by mechanical wounding at different times after treatment. POX22.3, POX8.1, and POX5.1 were estimated to be present in single copies in rice haploid genome. These results indicate that different members of the rice peroxidase gene family are distinctly regulated in response to various environmental cues.

scholarly journals First Report on the Transmission of ‘Candidatus Liberibacter americanus’ from Citrus to Nicotiana tabacum cv. Xanthi

Plant Disease ◽  
2007 ◽  
Vol 91 (5) ◽  
pp. 631-631 ◽  
Author(s):  
F. J. B. Francischini ◽  
K. D. S. Oliveira ◽  
G. Astúa-Monge ◽  
A. Novelli ◽  
R. Lorenzino ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Plant Species ◽  
Plant Pathogens ◽  
Control Plant ◽  
The United States ◽  
Pcr Product ◽  
Nicotiana Tabacum L ◽  
Electron Microscopy Analysis ◽  
Candidatus Liberibacter ◽  
Parasitic Effect

Huanglongbing (HLB), also known as greening, is one of the most important diseases of citrus worldwide. The causal agent is a gram-negative bacterium known to inhabit the phloem of infected plants. Three different candidate species infect citrus: ‘Candidatus Liberibacter africanus’ found in the African continent; ‘Ca. L. asiaticus’ found in Asia, Brazil, and the United States; and ‘Ca. L. americanus’ found in Brazil. (1). Tobacco is an easily transformable plant species that can be used as an experimental host system to quickly screen for candidate genes useful to control plant pathogens. However, no evidence exists on the ability of this plant species to sustain populations of ‘Ca. L. americanus’. With the purpose of transmitting ‘Ca. L. americanus’ from citrus to tobacco, fragments of healthy stems of Cuscuta spp. (dodder) were used to connect an HLB-infected sweet orange plant to each of 10 healthy plants of Nicotiana tabacum L. cv. Xanthi and allowed to remain connected for 30, 45, and 50 days. Three different HLB-infected orange plants and 30 tobacco plants were used in three independent experiments. Most HLB-exposed Xanthi plants exhibited chlorotic leaves after 50 days of exposure probably because of the parasitic effect of dodder; however, an average of 6, 1, and 3 Xanthi plants exhibited a unique blotchy mottle symptom after 30, 45, and 50 days of exposure, respectively. Symptomatic and asymptomatic leaves were collected and analyzed by PCR. The results consistently confirmed the presence of ‘Ca. L. americanus’ only in symptomatic leaves. Sequencing of the PCR product and comparison to the NCBI database also confirmed the identity of the pathogen as ‘Ca. L. americanus’. Electron microscopy analysis of four symptomatic leaves indicated the presence of bacterium-like bodies with round to elongated bacilliform shapes and surrounded by two membranes. These bodies resembled those already described in HLB-infected citrus in Brazil (1). The evidence presented above confirms the successful transmission of ‘Ca. L. americanus’ from citrus to Xanthi using the parasitic plant Cuscuta spp. Reference: (1) F. A. O. Tanaka et al. Fitopatol. Bras. 31:99, 2006.

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